scholarly journals Quantitative real-time PCR assay with immunohistochemical evaluation of HER2/neu oncogene in breast cancer patients and its correlation with clinicopathological findings

2020 ◽  
Vol 63 (5) ◽  
pp. 123
Author(s):  
Nadia Gheni ◽  
David Westenberg
Keyword(s):  
Breast Cancer ◽  
Real Time ◽  
Cancer Patients ◽  
Real Time Pcr ◽  
Breast Cancer Patients ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Clinicopathological Findings ◽  
Neu Oncogene

scholarly journals P4: CIRCULATING MIRNA PROFILES POINT TO DYSREGULATION IN TGFBETA/SMAD3 TUMOR SUPPRESSOR SIGNATURE IN BRCA POSITIVE BREAST CANCER PATIENTS

2016 ◽  
Vol 64 (3) ◽  
pp. 818.2-818
Author(s):  
I Shapira ◽  
T Bhuiya ◽  
S Arora ◽  
N Mukhi ◽  
S Datla ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tumor Suppressor ◽  
Real Time ◽  
Cancer Patients ◽  
Survival Data ◽  
Real Time Pcr ◽  
Germ Line ◽  
Germline Mutations ◽  
Breast Cancer Patients ◽  
Mean Differences

Purpose of StudyOver 240,000 individuals are diagnosed with breast cancer (BrCa) of which 12,000 individuals carry BRCA germline mutations. MicroRNA dysregulation is common in malignancy and may correlate with germline mutations.Aims:1. Analyze microRNAs in patients with breast cancer with or without BRCA germ line mutations, with and without cancer.2. Identify molecular BRCA mutant patients to deduct reasons for accelerated malignancy.Methods UsedWe analyzed plasma miR expression from 94 br cancer patients (41 BRCA positive) relative to 24 normal controls. All samples were collected between 2010 and 2014 and survival data was known for all cancer patients. TaqMan Open Array panel was used to simultaneously run hundreds of microRNA assays in the Applied Biosystem Open array real time PCR. Using AB open array real time PCR, 756 miRNA species were detected. Two-sample t-test was used for all 2-sample comparison and ANOVA followed by Tukey HSD post-hoc test to compare the miRs mean differences. All tests were 2-tailed and results with a p<0.05 were considered statistically significant.Summary of ResultsBRCA+underexpressed hsa-mir-10a and hsa-mir-376c and over-expressed Hsa- mir- 326 and Hsa-mir-143 relative to BRCA-; p<0.05.Using Coremine data mining linking genes and diseases differentially expressed circulating miRs are linked to tumor suppressor TGFbeta/SMAD3.ConclusionsThe early onset of breast cancer in BRCA mutant patients may recapitulate the pro-oncogenic effects of TGF-β. The context dependent SMAD3 binding & tumor suppression TGF-β effects are abrogated in BRCA mutant patients. TGF-β/Smad3 tumor-suppressor signature suppresses local inflammation in the tumor microenvironment.

Expression of hTERT in obese and normal body weight breast cancer patients by Real-Time PCR

2011 ◽  
Vol 44 (13) ◽  
pp. S71-S72
Author(s):  
Rahmati Mohammad ◽  
Zarghami Nosratallah ◽  
Pourhasan Mohammad ◽  
Nejati Kazem ◽  
Barkhordari Amin
Keyword(s):  
Breast Cancer ◽  
Body Weight ◽  
Real Time ◽  
Cancer Patients ◽  
Real Time Pcr ◽  
Breast Cancer Patients ◽  
Normal Body ◽  
Normal Body Weight

scholarly journals Quantitation of circulating DNA in the serum of breast cancer patients by real-time PCR

2004 ◽  
Vol 90 (6) ◽  
pp. 1211-1215 ◽  
Author(s):  
S Gal ◽  
C Fidler ◽  
Y M D Lo ◽  
M Taylor ◽  
C Han ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Real Time ◽  
Cancer Patients ◽  
Real Time Pcr ◽  
Circulating Dna ◽  
Breast Cancer Patients
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