Heat stress resistance effect of flavonoids from Penthorum chinense Pursh on Caenorhabditis elegans

2019 ◽  
Vol 15 (63) ◽  
pp. 514
Author(s):  
Chunbang Ding ◽  
Shiling Feng ◽  
Liang Fu ◽  
Yujie Wang ◽  
Handong Wang ◽  
...  
Keyword(s):  
Heat Stress ◽  
Caenorhabditis Elegans ◽  
Stress Resistance ◽  
Penthorum Chinense Pursh ◽  
Heat Stress Resistance ◽  
Penthorum Chinense

scholarly journals Genes implicated in Caenorhabditis elegans and human health regulate stress resistance and physical abilities in aged Caenorhabditis elegans

2021 ◽  
Vol 17 (6) ◽  
pp. 20200916
Author(s):  
Jessica Dysarz ◽  
Georg Fuellen ◽  
Steffen Möller ◽  
Walter Luyten ◽  
Christian Schmitz-Linneweber ◽  
...  
Keyword(s):  
Heat Stress ◽  
Caenorhabditis Elegans ◽  
Stress Resistance ◽  
Bioinformatics Analyses ◽  
C Elegans ◽  
Swim Performance ◽  
Health Related ◽  
Physical Abilities ◽  
Heat Stress Resistance ◽  
Literature Curation

Recently, nine Caenorhabditis elegans genes, grouped into two pathways/clusters, were found to be implicated in healthspan in C. elegans and their homologues in humans, based on literature curation, WormBase data mining and bioinformatics analyses. Here, we further validated these genes experimentally in C. elegans . We downregulated the nine genes via RNA interference (RNAi), and their effects on physical function (locomotion in a swim assay) and on physiological function (survival after heat stress) were analysed in aged nematodes. Swim performance was negatively affected by the downregulation of acox-1.1 , pept-1 , pak-2 , gsk-3 and C25G6.3 in worms with advanced age (twelfth day of adulthood) and heat stress resistance was decreased by RNAi targeting of acox-1.1 , daf-22 , cat-4 , pig-1 , pak-2 , gsk-3 and C25G6.3 in moderately (seventh day of adulthood) or advanced aged nematodes. Only one gene, sad-1 , could not be linked to a health-related function in C. elegans with the bioassays we selected. Thus, most of the healthspan genes could be re-confirmed by health measurements in old worms.

scholarly journals Mitochondrial Oxidative Stress Impairs Energy Metabolism and Reduces Stress Resistance and Longevity of C. elegans

2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Benjamin Dilberger ◽  
Stefan Baumanns ◽  
Fabian Schmitt ◽  
Tommy Schmiedl ◽  
Martin Hardt ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heat Stress ◽  
Caenorhabditis Elegans ◽  
Energy Metabolism ◽  
Mitochondrial Dysfunction ◽  
Respiratory Chain ◽  
Stress Resistance ◽  
Marker Genes ◽  
Heat Stress Resistance ◽  
Response Expression

Introduction. Mitochondria supply cellular energy and are key regulators of intrinsic cell death and consequently affect longevity. The nematode Caenorhabditis elegans is frequently used for lifespan assays. Using paraquat (PQ) as a generator of reactive oxygen species, we here describe its effects on the acceleration of aging and the associated dysfunctions at the level of mitochondria. Methods. Nematodes were incubated with various concentrations of paraquat in a heat-stress resistance assay (37°C) using nucleic staining. The most effective concentration was validated under physiological conditions, and chemotaxis was assayed. Mitochondrial membrane potential (ΔΨm) was measured using rhodamine 123, and activity of respiratory chain complexes determined using a Clark-type electrode in isolated mitochondria. Energetic metabolites in the form of pyruvate, lactate, and ATP were determined using commercial kits. Mitochondrial integrity and structure was investigated using transmission electron microscopy. Live imaging after staining with fluorescent dyes was used to measure mitochondrial and cytosolic ROS. Expression of longevity- and mitogenesis-related genes were evaluated using qRT-PCR. Results. PQ (5 mM) significantly increased ROS formation in nematodes and reduced the chemotaxis, the physiological lifespan, and the survival in assays for heat-stress resistance. The number of fragmented mitochondria significantly increased. The ∆Ψm, the activities of complexes I-IV of the mitochondrial respiratory chain, and the levels of pyruvate and lactate were significantly reduced, whereas ATP production was not affected. Transcript levels of genetic marker genes, atfs-1, atp-2, skn-1, and sir-2.1, were significantly upregulated after PQ incubation, which implicates a close connection between mitochondrial dysfunction and oxidative stress response. Expression levels of aak-2 and daf-16 were unchanged. Conclusion. Using paraquat as a stressor, we here describe the association of oxidative stress, restricted energy metabolism, and reduced stress resistance and longevity in the nematode Caenorhabditis elegans making it a readily accessible in vivo model for mitochondrial dysfunction.

scholarly journals Interactions with a Complex Microbiota Mediate a Trade-Off between the Host Development Rate and Heat Stress Resistance

2020 ◽  
Vol 8 (11) ◽  
pp. 1781
Author(s):  
Samuel Slowinski ◽  
Isabella Ramirez ◽  
Vivek Narayan ◽  
Medha Somayaji ◽  
Maya Para ◽  
...  
Keyword(s):  
Life History ◽  
Heat Stress ◽  
Stress Resistance ◽  
Life History Traits ◽  
Soil Microbes ◽  
Development Rate ◽  
Heat Stress Resistance ◽  
Host Life ◽  
Host Development ◽  
Resistance To Heat

Animals and plants host diverse communities of microorganisms, and these microbiotas have been shown to influence host life history traits. Much has been said about the benefits that host-associated microbiotas bestow on the host. However, life history traits often demonstrate tradeoffs among one another. Raising Caenorhabditis elegans nematodes in compost microcosms emulating their natural environment, we examined how complex microbiotas affect host life history traits. We show that soil microbes usually increase the host development rate but decrease host resistance to heat stress, suggesting that interactions with complex microbiotas may mediate a tradeoff between host development and stress resistance. What element in these interactions is responsible for these effects is yet unknown, but experiments with live versus dead bacteria suggest that such effects may depend on bacterially provided signals.

scholarly journals Proteomic analysis of heat stress resistance of cucumber leaves when grafted onto Momordica rootstock

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Ye Xu ◽  
Yinghui Yuan ◽  
Nanshan Du ◽  
Yu Wang ◽  
Sheng Shu ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Resistance ◽  
Proteomic Analysis ◽  
Heat Stress Resistance

Change in heat stress resistance in wheat due to soil nitrogen and atmospheric CO2levels

2007 ◽  
Vol 35 (2) ◽  
pp. 229-232 ◽  
Author(s):  
Szilvia Bencze ◽  
Eszter Keresztényi ◽  
Ottó Veisz
Keyword(s):  
Heat Stress ◽  
Soil Nitrogen ◽  
Stress Resistance ◽  
Heat Stress Resistance

scholarly journals Antiaging Effects of Vicatia thibetica de Boiss Root Extract on Caenorhabditis elegans and Doxorubicin-Induced Premature Aging in Adult Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Wenwen Liu ◽  
Yunhui Guan ◽  
Sicong Qiao ◽  
Jiqun Wang ◽  
Keting Bao ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Stress Resistance ◽  
Chinese Herb ◽  
Premature Aging ◽  
Lifespan Extension ◽  
Root Extract ◽  
Antioxidant Ability ◽  
C Elegans ◽  
Adult Mice ◽  
Heat Stress Resistance

The roots of Vicatia thibetica de Boiss are a kind of Chinese herb with homology of medicine and food. This is the first report showing the property of the extract of Vicatia thibetica de Boiss roots (HLB01) to extend the lifespan as well as promote the healthy parameters in Caenorhabditis elegans (C. elegans). For doxorubicin- (Doxo-) induced premature aging in adult mice, HLB01 counteracted the senescence-associated biomarkers, including P21 and γH2AX. Interestingly, HLB01 promoted the expression of collagen in C. elegans and mammalian cell systemically, which might be one of the essential factors to exert the antiaging effects. In addition, HLB01 was also found as a scavenger of free radicals, thereby performing the antioxidant ability. Lifespan extension by HLB01 was also dependent on DAF-16 and HSF-1 via oxidative stress resistance and heat stress resistance. Taken together, overall data suggested that HLB01 could extend the lifespan and healthspan of C. elegans and resist Doxo-induced senescence in mice via promoting the expression of collagen, antioxidant potential, and stress resistance.

SES1 positively regulates heat stress resistance in Arabidopsis

2019 ◽  
Vol 513 (3) ◽  
pp. 582-588 ◽  
Author(s):  
Peiyan Guan ◽  
Jun Wang ◽  
Chen Xie ◽  
Changai Wu ◽  
Guodong Yang ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Resistance ◽  
Heat Stress Resistance
Sign in / Sign up

Export Citation Format

Share Document