Effect of dried distillers’ grains and solubles when replacing corn or soybean meal on rumen microbial growth in vitro as measured using DNA as a microbial marker

2014 ◽  
Vol 94 (2) ◽  
pp. 349-356 ◽  
Author(s):  
E. Castillo-Lopez ◽  
T. J. Klopfenstein ◽  
S. C. Fernando ◽  
P. J. Kononoff

Castillo-Lopez, E., Klopfenstein, T. J., Fernando, S. C. and Kononoff, P. J. 2014. Effect of dried distillers’ grains and solubles when replacing corn or soybean meal on rumen microbial growth in vitro as measured using DNA as a microbial marker. Can. J. Anim. Sci. 94: 349–356. The objectives were to evaluate the use of rDNA markers to measure the effects of dried distillers’ grains with solubles (DDGS) and the potential treatment×time interaction on microbial crude protein (MCP) synthesis in vitro and secondly to measure the contribution of yeast based protein originating from DDGS. Treatments were: (1) CONT, control with no DDGS, but with alfalfa hay, corn silage, ground corn (GC) and soybean meal (SBM) included at 25% (DM basis); (2) LOWCORN, 20% DDGS (DM basis) replacing GC; (3) LOWSBM, 20% DDGS (DM basis) replacing SBM; and (4) LOWCORNSBM, 20% DDGS (DM basis) replacing 10% GC and 10% SBM. Treatments (0.5 g) were incubated in 50 mL of inoculum in duplicate. At 0, 4, 16, 32, 48 and 96 h of fermentation total DNA was extracted from each treatment and MCP was measured using rDNA markers. The sum of bacterial crude protein (BCP) and protozoal crude protein (PCP) was considered as MCP. Data were analyzed as a completely randomized design. The treatment×time interaction was tested and the SLICE option was included to evaluate the effect of treatment at each fermentation time point. There was a tendency to a treatment×time interaction (P=0.07) for MCP. Specifically, at 16 h, LOWCORNSBM yielded greater (P<0.05) MCP compared to either CONT or LOWCORN with estimates of 68.5, 33.8 and 23.3±8.9 mg g–1DM, for LOWCORNSBM, CONT and LOWCORN, respectively. At 48 h, however, LOWCORN yielded greater MCP (P<0.05) compared with LOWSBM with estimates of 72.2 and 32.5±8.9 mg g–1DM, for LOWCORN and LOWSBM, respectively. Yeast crude protein (YCP) was not affected (P=0.21) and averaged 0.04±0.02 mg g–1of substrate (DM basis). Overall, rDNA markers were effective for quantifying MCP, but further research on the methodology is needed. With DDGS inclusion, MCP was maintained; however, yeast cells were extensively degraded during fermentation.

2012 ◽  
Vol 55 (5) ◽  
pp. 496-505 ◽  
Author(s):  
M. Łukasiewicz ◽  
D. Pietrzak ◽  
J. Niemiec ◽  
J. Mroczek ◽  
M. Michalczuk

Abstract. Contemporarily, the production of high-grade ethyl alcohol enables obtaining dried distillers decoction that contains post-fermentation residues of cereal grains, solubles as well as yeast cells and their metabolites multiplied in the fermentation process, which makes that product applicable also in poultry feeding. Experiments were conducted on 540 broiler chickens of COBB 500 line. One-day chicks were randomly allocated to 3 nutritional groups: K, D1 and D2, each group consisted of 6 replicates of 30 birds. The factor that differentiated the groups was the content of dried wheat decoction in the starter type feed mixture (5% and 7%). Production results (individual body weight, feed intake and mortality) of the birds were controlled in a 42-day rearing period. On the 42nd day of rearing, 6 male and 6 female chickens from each group were chosen for slaughter that had body weights similar to the average of each group according to gender. The aim of this study was to determine experimentally whether the by-product of ethanol production is suitable for replacing soybean meal in feeding broiler chickens. The application of the wheat decoction had no negative effect on production results of the chickens. The birds fed a mixture with a higher content of dried distillers grains with solubles (DDGS) were characterised by a similar body weight and better feed conversion ratio compared to the control birds. A properly-balanced (fibre, energy, amino acids) nutritional dose of the dried wheat decoction may be used as a good energy-protein component in feed mixtures for broilers. It is a rational means of DDGS management which is, simultaneously, a cheaper substitute for soybean meal.


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