Development of a specific SCAR marker for the Ns genome of Psathyrostachys huashanica Keng

2014 ◽  
Vol 94 (8) ◽  
pp. 1441-1447
Author(s):  
Jing Wang ◽  
Wanli Du ◽  
Jun Wu ◽  
Xinhong Chen ◽  
Chanjuan Liu ◽  
...  
Keyword(s):  
Southern Hybridization ◽  
Scar Marker ◽  
Rapd Marker ◽  
Addition Lines ◽  
Psathyrostachys Huashanica ◽  
Base Pairs ◽  
Sequence Characterized Amplified Region ◽  
Complete Set ◽  
Low Costs ◽  
Disomic Addition Lines

Wang, J., Du, W., Wu, J., Chen, X., Liu, C., Zhao, J., Yang, Q. and Li, F. 2014. Development of a specific SCAR marker for the Ns genome of Psathyrostachys huashanica Keng. Can. J. Plant Sci. 94: 1441–1447. Psathyrostachys huashanica Keng (2n=2x=14, NsNs) possesses many agronomically desirable traits that could be used in wheat improvement. We have previously produced a complete set of wheat–P. huashanica disomic addition lines (1Ns–7Ns, 2n=44=22 II). To track the addition of P. huashanica chromatin in wheat rapidly and effectively, a repetitive sequence of 1665 base pairs, designated pHs8, was isolated based on 21 different Triticeae species, including the parents’ common wheat cv. 7182 and P. huashanica, by RAPD analysis. The diagnostic fragments of the RAPD marker OPF151665 were cloned, sequenced, and converted into a sequence-characterized amplified region (SCAR) marker, known as RHS12. Southern hybridization using labeled pHs8 as probe showed intense hybridization signals on P. huashanica, but not on the other 20 species at all. RHS12 was validated using 21 different plant species and a complete set of wheat–P. huashanica disomic addition lines. Our results indicated that the SCAR marker targeted the Ns genome of P. huashanica and it was present in all seven P. huashanica chromosomes. The newly developed SCAR marker should help wheat breeders to screen for genotypes containing P. huashanica chromatin with low costs and high throughput.

scholarly journals A DNA-Based Procedure for In Planta Detection of Fusarium oxysporum f. sp. phaseoli

2002 ◽  
Vol 92 (3) ◽  
pp. 237-244 ◽  
Author(s):  
Fernando M. Alves-Santos ◽  
Brisa Ramos ◽  
M. Asunción García-Sánchez ◽  
Arturo P. Eslava ◽  
José María Díaz-Mínguez
Keyword(s):  
Common Bean ◽  
Fusarium Oxysporum ◽  
Scar Marker ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
In Planta ◽  
Sequence Characterized Amplified Region ◽  
Polymerase Chain ◽  
Highly Virulent

We have characterized strains of Fusarium oxysporum from common bean fields in Spain that were nonpathogenic on common bean, as well as F. oxysporum strains (F. oxysporum f. sp. phaseoli) pathogenic to common bean by random amplified polymorphic DNA (RAPD) analysis. We identified a RAPD marker (RAPD 4.12) specific for the highly virulent pathogenic strains of the seven races of F. oxysporum f. sp. phaseoli. Sequence analysis of RAPD 4.12 allowed the design of oligonucleotides that amplify a 609-bp sequence characterized amplified region (SCAR) marker (SCAR-B310A280). Under controlled environmental and greenhouse conditions, detection of the pathogen by polymerase chain reaction was 100% successful in root samples of infected but still symptomless plants and in stem samples of plants with disease severity of ≥4 in the Centro Internacional de Agricultura Tropical (CIAT; Cali, Colombia) scale. The diagnostic procedure can be completed in 5 h and allows the detection of all known races of the pathogen in plant samples at early stages of the disease with no visible symptoms.

Development and characterization of a complete set of Triticum aestivum–Roegneria ciliaris disomic addition lines

2018 ◽  
Vol 131 (8) ◽  
pp. 1793-1806 ◽  
Author(s):  
Lingna Kong ◽  
Xinying Song ◽  
Jin Xiao ◽  
Haojie Sun ◽  
Keli Dai ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Addition Lines ◽  
Complete Set ◽  
Disomic Addition Lines

scholarly journals Characterization of a Resistance Locus (Pfs-1) to the Spinach Downy Mildew Pathogen (Peronospora farinosa f. sp. spinaciae) and Development of a Molecular Marker Linked to Pfs-1

2008 ◽  
Vol 98 (8) ◽  
pp. 894-900 ◽  
Author(s):  
B. M. Irish ◽  
J. C. Correll ◽  
C. Feng ◽  
T. Bentley ◽  
B. G. de los Reyes
Keyword(s):  
Disease Resistance ◽  
Downy Mildew ◽  
Scar Marker ◽  
Mapping Population ◽  
Dominant Gene ◽  
Resistance Locus ◽  
Sequence Characterized Amplified Region ◽  
Wide Range ◽  
Spinach Downy Mildew ◽  
Homozygous Resistant

Downy mildew is a destructive disease of spinach worldwide. There have been 10 races described since 1824, six of which have been identified in the past 10 years. Race identification is based on qualitative disease reactions on a set of diverse host differentials which include open-pollinated cultivars, contemporary hybrid cultivars, and older hybrid cultivars that are no longer produced. The development of a set of near-isogenic open-pollinated spinach lines (NILs), having different resistance loci in a susceptible and otherwise common genetic background, would facilitate identification of races of the downy mildew pathogen, provide a tool to better understand the genetics of resistance, and expedite the development of molecular markers linked to these disease resistance loci. To achieve this objective, the spinach cv. Viroflay, susceptible to race 6 of Peronospora farinosa f. sp. spinaciae, was used as the recurrent susceptible parent in crosses with the hybrid spinach cv. Lion, resistant to race 6. Resistant F1 progeny were subsequently backcrossed to Viroflay four times with selection for race 6 resistance each time. Analysis of the segregation data showed that resistance was controlled by a single dominant gene, and the resistance locus was designated Pfs-1. By bulk segregant analysis, an amplified fragment length polymorphism (AFLP) marker (E-ACT/M-CTG) linked to Pfs-1 was identified and used to develop a co-dominant Sequence characterized amplified region (SCAR) marker. This SCAR marker, designated Dm-1, was closely linked (≈1.7 cM) to the Pfs-1 locus and could discriminate among spinach genotypes that were homozygous resistant (Pfs-1Pfs-1), heterozygous resistant (Pfs-1pfs-1), or homozygous susceptible (pfs-1pfs-1) to race 6 within the original mapping population. Evaluation of a wide range of commercial spinach lines outside of the mapping population indicated that Dm-1 could effectively identify Pfs-1 resistant genotypes; the Dm-1 marker correctly predicted the disease resistance phenotype in 120 out of 123 lines tested. In addition, the NIL containing the Pfs-1 locus (Pfs-1Pfs-1) was resistant to multiple races of the downy mildew pathogen indicating Pfs-1 locus may contain a cluster of resistance genes.

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