Development and characterization of a complete set of Triticum aestivum–Roegneria ciliaris disomic addition lines

2018 ◽  
Vol 131 (8) ◽  
pp. 1793-1806 ◽  
Author(s):  
Lingna Kong ◽  
Xinying Song ◽  
Jin Xiao ◽  
Haojie Sun ◽  
Keli Dai ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Addition Lines ◽  
Complete Set ◽  
Disomic Addition Lines

Cytogenetic identification of Triticum peregrinum chromosomes added to common wheat

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 272-276 ◽  
Author(s):  
B. Friebe ◽  
E. D. Badaeva ◽  
B. S. Gill ◽  
N. A. Tuleen
Keyword(s):  
Triticum Aestivum ◽  
Key Words ◽  
Common Wheat ◽  
Addition Lines ◽  
Karyotypic Analysis ◽  
Chromosome Addition ◽  
C Banding ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Donor Species

C-banded karyotypes of a complete set of 14 Triticum peregrinum whole chromosome addition lines and 25 telosomic addition lines are reported. The added T. peregrinum chromosomes were not structurally rearranged compared with the corresponding chromosomes of the donor accession. Comprehensive karyotypic analysis confirmed Triticum umbellulatum as the donor species of the Uv genome and identified Triticum longissimum as the donor species of the Sv genome of T. peregrinum. Neither the Uv nor Sv genome chromosomes of the T. peregrinum accession showed large modifications when compared with the ancestral U and S1 genomes. Key words : Triticum aestivum, Triticum peregrinum, Triticum umbellulatum, Triticum longissimum, chromosome addition lines, C-banding.

Development of a complete set of Triticum aestivum-Aegilops speltoides chromosome addition lines

2000 ◽  
Vol 101 (1-2) ◽  
pp. 51-58 ◽  
Author(s):  
B. Friebe ◽  
L. L. Qi ◽  
S. Nasuda ◽  
P. Zhang ◽  
N. A. Tuleen ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Aegilops Speltoides ◽  
Addition Lines ◽  
Chromosome Addition ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Speltoides Chromosome

Development of a specific SCAR marker for the Ns genome of Psathyrostachys huashanica Keng

2014 ◽  
Vol 94 (8) ◽  
pp. 1441-1447
Author(s):  
Jing Wang ◽  
Wanli Du ◽  
Jun Wu ◽  
Xinhong Chen ◽  
Chanjuan Liu ◽  
...  
Keyword(s):  
Southern Hybridization ◽  
Scar Marker ◽  
Rapd Marker ◽  
Addition Lines ◽  
Psathyrostachys Huashanica ◽  
Base Pairs ◽  
Sequence Characterized Amplified Region ◽  
Complete Set ◽  
Low Costs ◽  
Disomic Addition Lines

Wang, J., Du, W., Wu, J., Chen, X., Liu, C., Zhao, J., Yang, Q. and Li, F. 2014. Development of a specific SCAR marker for the Ns genome of Psathyrostachys huashanica Keng. Can. J. Plant Sci. 94: 1441–1447. Psathyrostachys huashanica Keng (2n=2x=14, NsNs) possesses many agronomically desirable traits that could be used in wheat improvement. We have previously produced a complete set of wheat–P. huashanica disomic addition lines (1Ns–7Ns, 2n=44=22 II). To track the addition of P. huashanica chromatin in wheat rapidly and effectively, a repetitive sequence of 1665 base pairs, designated pHs8, was isolated based on 21 different Triticeae species, including the parents’ common wheat cv. 7182 and P. huashanica, by RAPD analysis. The diagnostic fragments of the RAPD marker OPF151665 were cloned, sequenced, and converted into a sequence-characterized amplified region (SCAR) marker, known as RHS12. Southern hybridization using labeled pHs8 as probe showed intense hybridization signals on P. huashanica, but not on the other 20 species at all. RHS12 was validated using 21 different plant species and a complete set of wheat–P. huashanica disomic addition lines. Our results indicated that the SCAR marker targeted the Ns genome of P. huashanica and it was present in all seven P. huashanica chromosomes. The newly developed SCAR marker should help wheat breeders to screen for genotypes containing P. huashanica chromatin with low costs and high throughput.

DISOMIC AND DITELOSOMIC ADDITIONS OF DIPLOID AGROPYRON ELONGATUM CHROMOSOMES TO TRITICUM AESTIVUM

1974 ◽  
Vol 16 (2) ◽  
pp. 399-417 ◽  
Author(s):  
J. Dvorak ◽  
D. R. Knott
Keyword(s):  
Triticum Aestivum ◽  
Chinese Spring ◽  
Gene Dosage ◽  
Plant Morphology ◽  
Addition Line ◽  
Addition Lines ◽  
Agropyron Elongatum ◽  
Monosomic Addition ◽  
Monosomic Addition Lines ◽  
Disomic Addition Lines

A set of disomic addition lines was produced in which each chromosome of Agropyron elongatum (2n = 14) was added to the chromosome complement of Triticum aestivum cv. Chinese Spring. In addition a complete set of ditelosomic addition lines involving Agropyron chromosome arms IS, IIα, IIIα, IVS, IVL, VS, VL, VIS and VIIα, was developed. Except for disomic addition line VII which is similar to Chinese Spring, each added alien chromosome pair was found to have a specific effect on plant morphology. Five disomic addition lines had reduced fertility. Agropyron chromosome arms carrying genes for anthocyanin production, waxless foliage, tenacious glumes, and several other traits were identified. The single Agropyron chromosomes in the monosomic addition lines usually have either minor effects or no effect on plant morphology and fertility.The disomic addition lines are similar in many characters to tetrasomics of the corresponding homoeologous wheat chromosomes and show characters that are not present in either Ag. elongatum or the amphiploid, T. aestivum × Ag. elongatum. Thus many of the effects are due to gene dosage. If individual disomic addition lines are compared with the amphiploid a significant role of interchromosomal gene interactions is apparent.In combination with knowledge of the homoeologous relationships among wheat and alien chromosomes, sets of disomic addition lines can provide valuable information on the evolutionary past of genomes in Triticinae. In monosomic addition lines only Agropyron chromosome IV paired with a wheat chromosome and it is concluded that it is improbable that Agropyron genomes played any role in the evolution of the polyploid series of Aegilops and Triticum.

Development and identification of a complete set of Triticum aestivum - Aegilops geniculata chromosome addition lines

Genome ◽  
1999 ◽  
Vol 42 (3) ◽  
pp. 374-380 ◽  
Author(s):  
Bernd R Friebe ◽  
Neal A Tuleen ◽  
Bikram S Gill
Keyword(s):  
Triticum Aestivum ◽  
Chromosome Breakage ◽  
Meiotic Pairing ◽  
Addition Lines ◽  
Aegilops Geniculata ◽  
Chromosome Addition ◽  
C Banding ◽  
Chromosome Addition Lines ◽  
Complete Set ◽  
Gametocidal Gene

The production and identification of a complete set of intact Aegilops geniculata chromosome and telosome additions to common wheat is described. All Ug and Mg genome chromosomes were tentatively assigned to their homoeologous groups based on C-banding, meiotic metaphase I pairing analyses and plant morphologies. Thirteen disomic and one monosomic wheat-Ae. geniculata chromosome additions were identified. Furthermore, two monotelosomic (MtA7UgL, MtA7MgL) and nine ditelosomic (DtA1UgS, DtA1UgL, DtA2UgS, DtA1MgL, DtA2MgL, DtA3MgS, DtA5MgS, DtA6MgL, DtA7MgS) wheat-Ae. geniculata additions were recovered. C-banding and meiotic pairing analyses revealed that all added Ug and Mg genome chromosomes are structurally unaltered compared to the Ae. geniculata parent accession. Chromosome 4Mg has a strong gametocidal gene that, when transferred to wheat, causes extensive chromosome breakage mainly in gametes lacking it. The relationships of Ae. geniculata chromosomes with those of the diploid progenitor species and derived polyploids is discussed.Key words: Triticum aestivum, Aegilops geniculata, chromosome addition lines, C-banding, genome evolution.

Molecular cytogenetic characterization ofAegilops biuncialisand its use for the identification of 5 derived wheat – Aegilops biuncialisdisomic addition lines

Genome ◽  
2005 ◽  
Vol 48 (6) ◽  
pp. 1070-1082 ◽  
Author(s):  
Annamária Schneider ◽  
Gabriella Linc ◽  
István Molnár ◽  
Márta Molnár-Láng
Keyword(s):  
Triticum Aestivum ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Genomic In Situ Hybridization ◽  
Addition Lines ◽  
Aegilops Umbellulata ◽  
Aegilops Comosa ◽  
Disomic Addition Lines ◽  
M Genome

The aim of the experiments was to produce and identify different Triticum aestivum – Aegilops biuncialis disomic addition lines. To facilitate the exact identification of the Ae. biuncialis chromosomes in these Triticum aestivum – Ae. biuncialis disomic additions, it was necessary to analyze the fluorescence in situ hybridization (FISH) pattern of Ae. biuncialis (2n = 4x = 28, UbUbMbMb), comparing it with the diploid progenitors (Aegilops umbellulata, 2n = 2x = 14, UU and Aegilops comosa, 2n = 2x = 14, MM). To identify the Ae. biuncialis chromosomes, FISH was carried out using 2 DNA clones (pSc119.2 and pAs1) on Ae. biuncialis and its 2 diploid progenitor species. Differences in the hybridization patterns of all chromosomes were observed among the 4 Ae. umbellulata accessions, the 4 Ae. comosa accessions, and the 3 Ae. biuncialis accessions analyzed. The hybridization pattern of the M genome was more variable than that of the U genome. Five different wheat – Ae. biuncialis addition lines were produced from the wheat – Ae. biuncialis amphiploids produced earlier in Martonvásár. The 2M, 3M, 7M, 3U, and 5U chromosome pairs were identified with FISH using 3 repetitive DNA clones (pSc119.2, pAs1, and pTa71) in the disomic chromosome additions produced. Genomic in situ hybridization (GISH) was used to differentiate the Ae. biuncialis chromosomes from wheat, but no chromosome rearrangements between wheat and Ae. biuncialis were detected in the addition lines.Key words: Triticum aestivum, Aegilops biuncialis, fluorescence in situ hybridization, genomic in situ hybridization, wheat – Aegilops biuncialis addition lines.

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