Adventitious shoot regeneration from leaves of apple rootstock ‘Pingyitiancha’ (Malus hupehensis var. pinyiensis) and genetic fidelity of regenerated plantlets using SSR markers

2014 ◽  
Vol 94 (8) ◽  
pp. 1345-1354 ◽  
Author(s):  
Wanmei Jin ◽  
Yuanhua Wang ◽  
Hua Wang
Keyword(s):  
Ssr Markers ◽  
Shoot Regeneration ◽  
Adventitious Shoots ◽  
Genetic Fidelity ◽  
Adventitious Shoot ◽  
Ms Medium ◽  
Adventitious Shoot Regeneration ◽  
Apple Rootstock ◽  
Malus Hupehensis ◽  
Regenerated Plantlets

Jin, W., Wang, Y. and Wang, H. 2014. Adventitious shoot regeneration from leaves of apple rootstock ‘Pingyitiancha’ (Malus hupehensis var. pinyiensis) and genetic fidelity of regenerated plantlets using SSR markers. Can. J. Plant Sci. 94: 1345–1354. Apple is one of the major fruit tree species in China, its cultivation area and total output rank first in the world. ‘Pingyitiancha’ (Malus hupehensis var. pinyiensis) is a widely used rootstock for apple cultivation in China. Several factors affecting leaf regeneration were investigated. In this study, a successful adventitious shoot regeneration protocol for this cultivar was established. ‘Pingyitiancha’ leaves were a suitable source of explants for regeneration of adventitious shoots. The optimal adventitious shoot regeneration protocol involved subculturing seedling leaves for 30–35 d. The optimum medium was Murashige and Skoog (MS) medium containing 2.0 mg L−1 thidiazuron and 0.2 mg L−1 indole-3-butyric acid. Explants with the abaxial surface in contact with the medium kept for 14 d in the dark showed the highest regeneration percentage of adventitious shoots of explants (100%), and produced an average of 3.6 shoots per regenerating explant. Shoots regenerated from leaves were rooted on half-strength MS medium containing 0.4 mg L−1 1-naphthalene acetic acid. The rooting percentage was 94.4%. Using SSR markers, all banding profiles from regenerated plantlets were monomorphic and same to those of the mother plant. It showed that the uniformity of the in vitro regenerated plantlets was maintained.

scholarly journals Adventitious Shoot Regeneration from Leaf Explant of Dwarf Hygro (Hygrophila polysperma(Roxb.) T. Anderson)

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Mehmet Karataş ◽  
Muhammad Aasim ◽  
Ayşegül Çınar ◽  
Muhammet Dogan
Keyword(s):  
Medicinal Plant ◽  
Shoot Regeneration ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Ms Medium ◽  
Adventitious Shoot Regeneration ◽  
Indian States ◽  
And Control ◽  
Regenerated Plantlets

Dwarf hygro (Hygrophila polysperma) is an ornamental aquatic plant that changes its leaf colours to pinkish in high light. It is listed as a medicinal plant in medicinal plant lists of Indian states of West Bengal and Karnataka. It is also used as a screening tool for toxicities and a bioindicator to detect and control algae. The study reportedin vitroadventitious shoot regeneration from leaf explants cultured on MS medium containing 0.10–1.60 mg/L Kin/TDZ with or without 0.10 mg/L IBA and 500 mg/L Amoklavin to eradicate endogenic bacterial contamination. Direct adventitious shoot regeneration started within one week from both culture mediums followed by late callus induction which was more prominent on TDZ containing media compared to Kin containing media. Addition of 0.10 mg/L IBA with both Kin and TDZ increased shoot regeneration frequency, mean number of shoots per explant, and mean shoot length. Maximum number of 16.33 and 20.55 shoots per explant was obtained on MS medium containing0.80+0.10 mg/L Kin-IBA and0.10+0.10 mg/L TDZ-IBA, respectively. Regenerated shoots were rooted on MS medium containing 0.20–1.00 mg/L IBA followed by successfull acclimatization in aquariums. Regenerated plantlets were also tested in jars containing distilled water that showed the pH 6–9 for the best plant growth and development.

scholarly journals Interaction between Explant Size and Cultivar Affects Shoot Organogenic Competence of Watermelon Cotyledons

HortScience ◽  
2000 ◽  
Vol 35 (4) ◽  
pp. 749-750 ◽  
Author(s):  
Michael E. Compton
Keyword(s):  
Shoot Regeneration ◽  
Significant Interaction ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Proximal Region ◽  
Specific Region ◽  
Adventitious Shoot Regeneration ◽  
Cotyledon Explants

Organic competence of different explant sizes and locations on watermelon seedlings was determined by calculating the percentage of cotyledon explants that produced adventitious shoots. About 52% (214/412) of explants prepared from the proximal region of cotyledons formed shoots, whereas only ≈6% (24/411) of distal explants did so. Shoot formation was limited to the proximal end of basal explants but was not restricted to any specific region on distal ones. The percentage of explants that produced harvestable shoots was greater from basal halves than basal quarters in `Sweet Gem', `Crimson Sweet', and `Minilee', but explant size did not affect adventitious shoot regeneration of `Yellow Doll', resulting in significant interaction between cultivar and explant size. This study indicates that cultivars that respond poorly to in vitro procedures may have fewer cells competent for shoot regeneration, requiring special care during explant preparation.

scholarly journals Tissue culture of Cecropia glaziovii Sneth (urticaceae): vegetative micropropagation and plant regeneration from callus

2010 ◽  
Vol 34 (5) ◽  
pp. 1245-1252 ◽  
Author(s):  
Marcos Nopper Alves
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Shoot Regeneration ◽  
Culture Media ◽  
Adventitious Shoot ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Adventitious Shoot Regeneration ◽  
Popular Medicine ◽  
Superior Genotype

Cecropia glaziovii is a tree with used in Brazilian popular medicine. Methods allowing the clonal propagation of this species are of great interest for superior genotype multiplication and perpetuation. For this reason, we examined the effect of different culture media and different types of explants on adventitious shoot regeneration from callus and buds of C. glaziovii. Leaves, petioles and stipules obtained from aseptically grown seedlings or from pre-sterilized plants were used to initiate cultures. Adventitious shoot regeneration was achieved when apical and axillary buds were inoculated on gelled Murashige & Skoog (MS) medium supplemented with 6-benzylaminopurine alone (BAP) (1.0, 5.0 or 10.0 mg L-1) or combined with -naphthalene acetic acid (NAA) (1.0 or 2.0 mg L-1), after 40 days of culture. Best callus production was obtained after 30 days of petioles' culture on gelled MS medium with 2,4 dichlorophenoxyacetic acid (2,4-D) (5.0 mg L-1) combined with BAP (1.0 mg L-1). Successful shoot regeneration from callus was achieved when MS medium supplemented with zeatin (ZEA) (0.1 mg L-1) alone or combined with 2,4-D (1.0 or 5.0 mg L-1) was inoculated with friable callus obtained from petioles. All shoots were rooted by inoculation on MS medium supplemented with indole-3-acetic acid (IAA) (1.0 mg L-1). Rooted plants transferred to potting soil were successfully established. All in vitro regenerated plantlets showed to be normal, without morphological variations, being also identical to the source plant. Our study has shown that C. glaziovii can be propagated by tissue culture methods, allowing large scale multiplication of superior plants for pharmacological purposes.

Adventitious shoot regeneration from dormant buds of persimmon (Diospyros kaki Thunb.) cv. Hachiya

2011 ◽  
Vol 28 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Zuzana Kochanová ◽  
Naci Onus ◽  
Ján Brindza
Keyword(s):  
Shoot Regeneration ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Hormone Treatment ◽  
Mediterranean Area ◽  
Shoot Length ◽  
Diospyros Kaki ◽  
Adventitious Shoot Regeneration ◽  
Significant Difference

Adventitious shoot regeneration from dormant buds of persimmon (Diospyros kakiThunb.) cv. HachiyaThe effects of plant hormones 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) on adventitious shoot regeneration from dormant persimmon buds were studied. The object of the study was the persimmon cultivar Hachiya, one of the most important persimmon cultivars in the Mediterranean area and Asia. Shoot regeneration was evaluated 4, 6 and 8 weeks after initiating the hormone treatment. Average shoot length was measured after 8 weeks and was evaluated by LSD test. Except for the media without hormone supplement, there was a statistically significant difference among average values of shoot length of plants, grown on the tested media. The increase in BAP had an effect on shoot regeneration that was significant and more pronounced with the addition of IBA, especially to the MS (1/2 N) medium. The highest value of shoot regeneration (98%) was obtained on medium MS (1/2 N), supplemented with 5 μmol l-1BAP and 1 μmol l-1IBA, with the highest average shoot length 23.69 mm, measured 8 weeks after the experiment initiation. The results indicate that adventitious shoots can be successfully produced in persimmon cv. Hachiya, especially with the supplement of hormone BAP, which, according to our results, plays an important role in persimmonin vitroregeneration.

scholarly journals Sodium Nitroprusside Stimulates Micropropagation and TDZ Induces Adventitious Shoots Regeneration in Red Flesh Apple Malus niedzwetzkyana Koehne Dieck ex

2019 ◽  
Vol 27 (2) ◽  
pp. 23-30
Author(s):  
Nooshin Kazemi ◽  
Maryam Jafarkhani Kermani ◽  
Ali Akbar Habashi
Keyword(s):  
Sodium Nitroprusside ◽  
Shoot Regeneration ◽  
Exposure Time ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Interactive Effects ◽  
Naphthalene Acetic Acid ◽  
Adventitious Shoot Regeneration ◽  
Basal Section

AbstractThe aim of the present investigation was to optimize protocols for micropropagation and adventitious shoot regeneration from leaf explants of two wild ecotypes of red flesh apple Malus niedzwetzkyana for future breeding programs. At the proliferation stage, different concentrations of sodium nitroprusside (SNP) and triacontanol (TRIA) were compared. To optimize shoot regeneration from leaf explants, interactive effects of 1-phenyl-3-(1,2,3-thidiazol-5-yl)-urea – thidiazuron (TDZ), indole-3-butyric acid (IBA) and two explant types were investigated. At rooting stage, the effect of exposure time of microshoots to darkness and exposure time to different concentrations of IBA and α-naphthalene acetic acid (NAA) were compared. The results showed that SNP affected the growth rate significantly and the maximum multiplication rates per explant (9.6 in the first ecotype and 8.8 in the second) were produced in the Quoirin and Lepoivre medium containing 17 SNP µM, in addition to 4 µm 6-benzylaminopurine (BAP) and 3 µm gibberellic acid (GA3). IBA and TDZ affected the adventitious shoot regeneration from leaf explants significantly, the highest number of regenerated shoots (18.3 per explant) was obtained from the basal section of the leaves cultured on the medium containing 2 μM IBA and 15 μM TDZ. At rooting stage, the maximum rooting (88.6%) was obtained in the result of one weak exposure to darkness on medium containing 3 μM IBA.

scholarly journals Adventitious Shoot Regeneration and Plant Production from Explants of Apios americana

HortScience ◽  
1990 ◽  
Vol 25 (11) ◽  
pp. 1436-1439 ◽  
Author(s):  
E.R.M. Wickremesinhe ◽  
W.J. Blackmon ◽  
B.D. Reynolds
Keyword(s):  
Shoot Regeneration ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Plant Production ◽  
Ms Medium ◽  
Adventitious Shoot Regeneration ◽  
Butanoic Acid ◽  
Apios Americana ◽  
Efficient Regeneration

Shoots were regenerated from callus of Apios americana Medikus (apios, groundnut) using internodal explants from in vitro-germinated seedlings and from sprouted tubers on a modified Murashige and Skoog (MS) basal medium. Shoot regeneration was observed over a range of 2iP and IBA combinations. GA3 increased the number of shoots regenerated per epicotyl explant. The most efficient regeneration (≈90%) was with internodal epicotyl explants on 100 μm 2iP, 0.5 μm IBA, and 1.5 μm GA3. Regenerated shoots were rooted on liquid and solid MS medium with 0.5 μm IBA; however, rooting was more successful on the liquid medium. About 60% of rooted plants were successfully established in pots. Chemical names used: N-(3-methyl2-butenyl)-1 H-purin-6-amine (2iP), 1 H-indole-3-butanoic acid (IBA), gibberellic acid (G A3).

scholarly journals Reinvigoration of diploid strawberry (Fragaria vesca) during adventitious shoot regeneration

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hua Wang ◽  
Yuan Yang ◽  
Maofu Li ◽  
Jiashen Liu ◽  
Wanmei Jin
Keyword(s):  
Functional Genomics ◽  
Shoot Regeneration ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Fragaria Vesca ◽  
Adventitious Shoot Regeneration ◽  
Hormone Synthesis ◽  
Dark Culture ◽  
Diploid Strawberry

Abstract Diploid strawberry (Fragaria vesca ‘Baiguo’) is a model plant for studying functional genomics in Rosaceae. Adventitious shoot regeneration is essential for functional genomics by Agrobacterium tumefaciens-mediated transformation. An efficient shoot regeneration method using diploid strawberry leaf explants was conducted on 1/2MS + 1/2B5 medium that contained 2.0 mg L−1 TDZ over 14 days of dark culture; this induced the maximum percentage of shoot regeneration (96.44 ± 1.60%) and the highest number of shoots per explant (23.46 ± 2.14) after 11 weeks of culture. The explants considerably enlarged after 12 days; then, turned greenish brown after 30 days, yellowish brown after 36 days, and completely brown and necrotic after 48 days. Large numbers of adventitious shoots were produced from 48 to 66 days, and the shoots elongated from 66 to 78 days; this represents a critical period of reinvigoration, which included 30 days for leaf explant chlorosis, 36 days for adventitious shoot appearance, and 48 days for generation of numerous shoots. During the reinvigoration process, higher expressions of the hormone synthesis-related genes Ciszog1, CKX2, CKX3, CKX7, YUC2, YUC6, YUC10, YUC9, and GA2ox were detected from 30 to 48 days. Our results indicate that these genes may regulate reinvigoration of shoot regeneration.

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