Prevention of shoot vitrification of strawberry micropropagated shoots proliferated on liquid media by new antivitrifying agents

1993 ◽  
Vol 73 (1) ◽  
pp. 231-235 ◽  
Author(s):  
Chafik Hdider ◽  
Yves Desjardins
Keyword(s):  
Liquid Medium ◽  
Key Words ◽  
Agar Medium ◽  
High Rate ◽  
Fragaria Ananassa ◽  
Liquid Media ◽  
Cellulose Plugs

Micropropagated strawberry (Fragaria × ananassa Duch. ’Kent.’) cultured for 4 wk in liquid proliferation media supported on cellulose plugs (sorbarods) developed a high rate of vitrification. This high percentage of abnormal plantlets was significantly reduced by the inclusion of two antivitrifying agents, EM1 and EM2, to the liquid medium. Virtually no vitrification occurred at 5 g L−1of EM1 or EM2. Shoots multiplied on liquid medium supplemented with 5 g L−1 of either EM1 or EM2 had significantly lower fresh weights and lower multiplication rates than on solidified agar medium (0.7%). EM2 produced better growth and proliferation than EM1 or liquid medium alone and was easier to use than EM1. Key words: Vitrification, antivitrifying agent, liquid medium

THE SIGNIFICANCE OF THE NUMBER OF PRECIPITIN BANDS IN AGAR GEL MEDIUM FOR THE DETERMINATION OF THE COMPLEXITY OF ANTIGENS

1958 ◽  
Vol 36 (11) ◽  
pp. 1105-1113 ◽  
Author(s):  
M. Richter ◽  
B. Rose ◽  
A. H. Sehon
Keyword(s):  
Liquid Medium ◽  
Agar Medium ◽  
Model System ◽  
Aminobenzoic Acid ◽  
Liquid Media ◽  
Agar Gel ◽  
Precipitin Test ◽  
Gel Technique ◽  
New Interpretation

A new interpretation is presented to account for inconsistancies which may arise when comparing data obtained with antibody–antigen systems in agar and in liquid media. For a model system, different haptens (p-aminobenzoic acid and p-sulphanilic acid) coupled to proteins were used. It has been demonstrated that the number of precipitin bands formed in agar medium by these antibody–antigen systems may be smaller than the number of optimal proportion zones detected for these systems in liquid medium, provided the different haptens are coupled to the same carrier. This effect is not due to a lesser sensitivity of the reaction in agar but rather to the inherent limitations of this method. Thus, by the agar technique, not more than one antigenic moiety (present on a molecule) is detected, whereas by the precipitin test in liquid medium optimal proportion zones with respect to each of the antigenic moieties (on the molecule) may be detected. This limitation in agar is not considered to detract from the value of the agar gel technique when it is used for the detection of independent antigens.

THE SIGNIFICANCE OF THE NUMBER OF PRECIPITIN BANDS IN AGAR GEL MEDIUM FOR THE DETERMINATION OF THE COMPLEXITY OF ANTIGENS

1958 ◽  
Vol 36 (1) ◽  
pp. 1105-1113
Author(s):  
M. Richter ◽  
B. Rose ◽  
A. H. Sehon
Keyword(s):  
Liquid Medium ◽  
Agar Medium ◽  
Model System ◽  
Aminobenzoic Acid ◽  
Liquid Media ◽  
Agar Gel ◽  
Precipitin Test ◽  
Gel Technique ◽  
New Interpretation

A new interpretation is presented to account for inconsistancies which may arise when comparing data obtained with antibody–antigen systems in agar and in liquid media. For a model system, different haptens (p-aminobenzoic acid and p-sulphanilic acid) coupled to proteins were used. It has been demonstrated that the number of precipitin bands formed in agar medium by these antibody–antigen systems may be smaller than the number of optimal proportion zones detected for these systems in liquid medium, provided the different haptens are coupled to the same carrier. This effect is not due to a lesser sensitivity of the reaction in agar but rather to the inherent limitations of this method. Thus, by the agar technique, not more than one antigenic moiety (present on a molecule) is detected, whereas by the precipitin test in liquid medium optimal proportion zones with respect to each of the antigenic moieties (on the molecule) may be detected. This limitation in agar is not considered to detract from the value of the agar gel technique when it is used for the detection of independent antigens.

PROGNOSTIC VALUE OF COMBINATION AIMS65 AND MELD SCORES IN CIRRHOTIC PATIENTS WITH ACUTE VARICEAL BLEEDING

2019 ◽  
pp. 35-40
Author(s):  
Thi Nhung Nguyen ◽  
Trung Nam Phan ◽  
Van Huy Tran
Keyword(s):  
Clinical Practice ◽  
Portal Hypertension ◽  
Key Words ◽  
Variceal Bleeding ◽  
Prognostic Value ◽  
High Rate ◽  
Central Hospital ◽  
Acute Variceal Bleeding ◽  
Cirrhotic Patients ◽  
Sensitivity Specificity

Bacground: Variceal bleeding is a severe complication of portal hypertension due to cirrhosis with high rate of motality, hence, predicting early rebleeding and mortality in cirrhotic patients with acute variceal bleeding is vital in clinical practice. Objectives: To evaluate the prognostic value of the combination of AIMS65 and MELD scores in predicting first 5 days in-hospital rebleeding and mortality in cirrhotic patients with acute variceal bleeding. Materials and Methods: 44 cirrhotic patients with acute variceal bleeding hospitalized at Hue Central Hospital. MELD and AIMS65 scores were calculated within the first 24 hours and monitoring rebleeding and mortality in the first 5 days in these patients. Results: AIMS65, MELD scores can predict first 5 days rebleeding and mortality with AUROC are 0.81, 0.69 and 0.92, 0.95, respectively. Combination of AIMS65 and MELD scores can predict first 5 days in hospital rebleeding with AUROC is 0.84, sensitivity 83.3%, specificity 81.6% (p<0.001) and mortality with AUROC is 0.96, sensitivity 100%, specificity 92.7% (p<0.001). Conclusions: The combination of AIMS65 and MELD scores increased the sensitivity, specificity and prognostic value in predicting first 5 days in-hospital rebleeding and mortality in cirrhotic patients with acute variceal bleeding in compare to each single scores. Key words: AiMS65 score, MELd, acute variceal bleeding

Glooscap and Blomidon strawberries

1991 ◽  
Vol 71 (3) ◽  
pp. 937-941
Author(s):  
D. L. Craig ◽  
A. R. Jamieson ◽  
K. A. Sanford
Keyword(s):  
Key Words ◽  
Fragaria Ananassa ◽  
Fruit Breeding ◽  
Fresh Market ◽  
Late Season ◽  
Short Day

Glooscap and Blomidon are short-day strawberry (Fragaria × ananassa Duch.) cultivars which produce large fruit suited to the fresh market. Glooscap, which is particularly high yielding and winter hardy, ripens in midseason whereas Blomidon, which is particularly glossy and attractive, ripens mid- to late season. Key words: Fragaria × ananassa, fruit breeding, strawberry

Evaluation of low temperature hardiness of strawberry plants under field and controlled conditions

1993 ◽  
Vol 73 (4) ◽  
pp. 1123-1125 ◽  
Author(s):  
J. M. Turner ◽  
K. K. Tanino ◽  
C. Stushnoff
Keyword(s):  
Low Temperature ◽  
Key Words ◽  
Scoring System ◽  
Growth Habit ◽  
Field Conditions ◽  
Mother Plant ◽  
Fragaria Ananassa ◽  
Plant Survival ◽  
Field Growth ◽  
Mother Plants

When acclimated strawberry plants (Fragaria × ananassa Duch. ’Redcoat’) from the field were placed in a programmable freezer, mother strawberry crowns were as hardy or hardier than daughter crowns when frozen to −11 °C. However, under field conditions, daughter plants expressed consistently greater hardiness than mother plants. Mulching had no effect on daughter plant survival, whereas enhanced mother plant survival was observed. Field-growth habit of mother crowns may be the important factor for consistent winter survival under prairie conditions. A simple scoring system was developed to predict survival of strawberry plants. Key words. Fragaria × ananassa Duch., strawberry, crowns, low temperature hardiness

Enumeration of petroleum-degrading marine and estuarine microorganisms by the most probable number method

1978 ◽  
Vol 24 (5) ◽  
pp. 552-557 ◽  
Author(s):  
A. L. Mills ◽  
C. Breuil ◽  
R. R. Colwell
Keyword(s):  
Crude Oil ◽  
Liquid Medium ◽  
Silica Gel ◽  
Agar Medium ◽  
Most Probable Number ◽  
Probable Number ◽  
Petroleum Degraders

Several media designed for use in a most probable number (MPN) determination of petroleum-degrading microorganisms were compared. The best results, i.e., largest numbers, were obtained using a buffered (32 mM PO4≡) liquid medium containing 1% hydrocarbon substrate. Of 104 presumptive oil degraders tested, 20 grew on oil agar medium but did not utilize oil or a mixture of pure paraffinic hydrocarbons (C10 to C16n-alkanes) in liquid (MPN) medium. Visible turbidity in the liquid medium was correlated with hydrocarbon utilization. Counts of petroleum degraders obtained using liquid medium (MPN) were in most cases higher than those obtained on an oil-amended silica gel medium. Both procedures yield an estimation of oil degraders, and the oil-amended agar permits growth of organisms which do not degrade crude oil. All strains of oil-degrading microorganisms examined in this study were lipolytic, but the converse was not always true.

Gene expression associated with light-induced conidiation in Colletotrichum graminicola

1991 ◽  
Vol 37 (2) ◽  
pp. 165-167 ◽  
Author(s):  
Zhenfan Yang ◽  
Daniel G. Panaccione ◽  
Robert M. Hanau
Keyword(s):  
Gene Expression ◽  
Key Words ◽  
Agar Medium ◽  
Cell Development ◽  
Conidiogenous Cell ◽  
In Vitro Translation ◽  
Asexual Development ◽  
Light Induction ◽  
Colletotrichum Graminicola

Light was used to induce conidiation in uniform populations of vegetative hyphae of Collectotrichum graminicola grown on agar medium. Differentiation of conidiogenous cells, the first detectable event in conidial morphogenesis, was rapid and highly synchronized. In vitro translation of poly(A)+ RNA from dark-grown (nondifferentiating) and light-induced (differentiating) hyphae demonstrated that conidiogenous cell development was accompanied by detectable changes in gene expression. Key words: Colletotrichum, conidia, asexual development, conidiogenous cell, light induction.

scholarly journals An Efficient Method of Pennisetum x Advena ‘Rubrum’ Seedlings Production Using Temporary Immersion Bioreactor and Agar Cultures

2021 ◽  
Author(s):  
Mariusz Pożoga ◽  
Dawid Olewnicki ◽  
Elżbieta Wójcik-Gront
Keyword(s):  
Liquid Medium ◽  
Efficient Method ◽  
Activated Charcoal ◽  
Agar Medium ◽  
Ms Medium ◽  
Temporary Immersion Bioreactor ◽  
Temporary Immersion ◽  
Bright Green ◽  
Bioreactor System

Abstract The aim of this study was to propose an efficient method of Pennisetum x advena ‘Rubrum’ micropropagation. Agar cultures with MS medium supplemented with BAP in various concentrations (0.5 mg/L-2 mg/L) and a temporary immersion bioreactor system (TIS) with liquid medium MS with an addition of 1 mg/L BAP were used. For rooting ½ MS medium with different auxin combinations (IBA, NAA) and activated charcoal was utilized. The most efficient method turned out to be TIS which produced 36.9 new plants in four weeks. The seedlings were slender in shape, bright green in colour with no signs of hyperhydricity. The most suitable agar medium produced 19.5 new plants in an eight week period. Rooting should be carried on ½ MS supplemented with 0.5 mg/L IBA and 0.5 mg/L NAA with an 84% rooting rate. The addition of activated charcoal inhibited rooting.

scholarly journals MICROPROPAGATION OF MUSA ON A STATIONARY LIQUID MEDIUM

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 696g-696
Author(s):  
Joan P. Harris
Keyword(s):  
Liquid Medium ◽  
Mutation Rate ◽  
Multiplication Rate ◽  
In Vitro Growth ◽  
Liquid Media ◽  
Stationary Liquid ◽  
One Year

In vitro growth of banana, Musa sp., using a modified Murashige and Skoog liquid medium was compared with the same formulation solidified by the addition of agar. Plants grown on the stationary liquid weighed more and had a higher multiplication rate. The best volume of liquid and number of plants per vessel were determined. Four hundred plants grown in liquid media were studied for one year after planting in the field. Flowering occurred at 7 months and harvesting 3 months later. The mutation rate observed was below 5%.

scholarly journals Cellulase activity of a Lentinula edodes (Berk.) Pegl. strain grown in media containing Carboximetilcellulose or microcrystalline cellulose

2003 ◽  
Vol 46 (3) ◽  
pp. 333-337 ◽  
Author(s):  
José Antônio de Sousa Pereira Júnior ◽  
Marcos José Correia ◽  
Neiva Tinti de Oliveira
Keyword(s):  
Enzyme Activity ◽  
Liquid Medium ◽  
Microcrystalline Cellulose ◽  
Lentinula Edodes ◽  
Cellulase Activity ◽  
Maximum Activity ◽  
Liquid Media

Endoglucanase and exocellobiohydrolase produced b Lentinula edodes (Berk.) Pegl. strain thatt was cultivated in carboxymetilcellulose (CMC) or microcrystalline cellulose (Avicel) liquid media. The concentration and type of cellulose influenced the enzyme activity and production. Extra-cellular cellobiase activity was not detected in CMC or Avicel media. This enzyme was detected in mycelial extracts only. With 1.7% Avicel liquid medium, the strain did not produce exocellobiohydrolase, but 74 µmol RBBR/mg protein/min was detected with 0.5% Avicel. The substitution of Avicel by 0.5% CMC reduced this activity. Endoglucanase also had maximum activity in 0.5% Avicel medium (approximately 820 UI/mg protein) after 96 h incubation. In supernatants from 0.5% CMC, the maximum activity attained was 200 UI/mg protein only.

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