scholarly journals MICROPROPAGATION OF MUSA ON A STATIONARY LIQUID MEDIUM

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 696g-696
Author(s):  
Joan P. Harris
Keyword(s):  
Liquid Medium ◽  
Mutation Rate ◽  
Multiplication Rate ◽  
In Vitro Growth ◽  
Liquid Media ◽  
Stationary Liquid ◽  
One Year

In vitro growth of banana, Musa sp., using a modified Murashige and Skoog liquid medium was compared with the same formulation solidified by the addition of agar. Plants grown on the stationary liquid weighed more and had a higher multiplication rate. The best volume of liquid and number of plants per vessel were determined. Four hundred plants grown in liquid media were studied for one year after planting in the field. Flowering occurred at 7 months and harvesting 3 months later. The mutation rate observed was below 5%.

scholarly journals Evaluation of Staphylococcal Activity of Garcinia kola Almonds

2019 ◽  
pp. 1-7
Author(s):  
Ouattara Karamoko ◽  
Dibi Koffi Saint Didier ◽  
Kone Monon ◽  
Ouattara Abou ◽  
Bagre Issa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Reference Strain ◽  
Susceptibility Testing ◽  
Traditional Society ◽  
In Vitro Growth ◽  
Liquid Media ◽  
Cost Of Treatment ◽  
Garcinia Kola

The emergence of infectious diseases, particularly staphylococcal infections, treatment failures and the more high cost of treatment of infections caused by resistant staphylococci called to find other care alternatives. This study was initiated to evaluate the antibacterial activity of the aqueous extract from Garcinia kola almonds on the in vitro growth of Staphylococcus aureus strains. The methods of diffusion in agar and liquid media were used for susceptibility testing and MIC and MBC determination. The tests were performed on four strains of S. aureus and one reference strain. The minimum inhibitory concentrations of the extracts ranged from 3.12 mg/mL and 12.5 mg/mL and the minimum bactericidal concentrations between 6.25 mg/mL and 25 mg/mL. The lowest value of MIC and MBC was observed with S. aureus ATCC 29213 while the greatest value of these same parameters was obtained on S. aureus 993C/18 and S. aureus 1075C/18. The aqueous almonds extract of Garcinia kola had a bactericidal activity on all the strains of S. aureus studied. This could justify the use of Garcinia kola almonds in the treatment of various diseases in traditional society.

scholarly journals Static Liquid Culture of Doritaenopsis Seedlings

HortScience ◽  
2008 ◽  
Vol 43 (1) ◽  
pp. 206-210 ◽  
Author(s):  
Wei-Ting Tsai ◽  
Chien-Young Chu
Keyword(s):  
Liquid Medium ◽  
Liquid Culture ◽  
Petri Dish ◽  
Liquid Media ◽  
Solid Culture ◽  
Sealing Material ◽  
Phase Liquid ◽  
Sealing Materials ◽  
Growth Of Seedlings

Methods for static liquid culture are described to improve the growth of Doritaenopsis (commercially known as Phalaenopsis) seedlings in vitro. The results showed that seeds not only germinated, but also grew faster in liquid medium. No hyperhydric seedlings were observed in liquid culture when liquid level was accurately controlled by culture density, medium volume, and sealing materials. Although the germination percent was unaffected by medium phase (liquid or solid), sowing density, medium volume, or sealing material, the growth of seedlings decreased as density increased or medium volume decreased. Seeds of 1.5 mg mixed with 20 mL of liquid medium per 9-cm petri dish sealed with two layers of parafilm prompted optimal results. Shoot growth also was enhanced while 75-day-old seedlings were subcultured in liquid media with or without support. Seedling growth was enhanced by adding 20 mL liquid media to 36 seedlings without support after 45 days of culture. It was expected that by static liquid culture, the period from sowing to ex vitro would be 1.5 months shorter than the traditional solid culture.

In vitro growth and differentiation of Gladiolus plants from callus cultures

1971 ◽  
Vol 49 (10) ◽  
pp. 1817-1819 ◽  
Author(s):  
J. Simonsen ◽  
A. C. Hildebrandt
Keyword(s):  
Vascular Tissue ◽  
Callus Cultures ◽  
In Vitro Growth ◽  
Liquid Media ◽  
Callus Production

Callus was induced from isolated gladiolus cormel stem tips on modified Murashige and Skoog media. The callus was then induced to differentiate virus-symptomless plants with corms. Morphogenesis from the callus included meristematic zones on or within the callus, organoids with vascular tissue, stems, leaves, and plants with roots. Callus production and differentiation of plants were more frequent on agar than in liquid media.

scholarly journals IN VITRO GROWTH RATE OF Kappaphycus alvarezii MICROPROPAGULE AND EMBRYO BY ENRICHMENT MEDIUM WITH SEAWEED EXTRACT

2015 ◽  
Vol 10 (1) ◽  
pp. 13
Author(s):  
Emma Suryati ◽  
Rosmiati Rosmiati ◽  
Andi Parenrengi ◽  
Andi Tenriulo
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Liquid Medium ◽  
Callus Induction ◽  
Kappaphycus Alvarezii ◽  
Seaweed Extract ◽  
In Vitro Growth ◽  
Seaweed Cultivation ◽  
Enrichment Medium

The development of micropropagule and embryo of seaweed depend on nutrient and fertilizer used. Seaweed has been reported contain hormone regulators such as auxine, cytokinine, gibbereline, and various minerals applied in stimulating the growth ocra plant and wheat culture. The objectives of this study were to determine the potential of Kappaphycus alvarezii extract and its optimal concentration in accelerating of Kappaphycus alvarezii micropropagule and embryo growth. Micropropagule and embryo produced through callus induction were planted into PES 1/20 liquid medium supplemented with seaweed extract at the concentrations of 0 (control), 25, 50, 75, and 100 μL in 10 mL of medium. The results showed that medium enrichment with 50 μL of seaweed extract had the highest survival rate and growth of thallus. In addition, this concentration was also resulted in a good performance of K. alvarezii thallus with the lighter color. The advantage of this study for seaweed cultivation in Indonesia, among others, seaweed can be used as fertilizer, especially in the maintenance of seaweed seed, so that cultivation can be better develop.

scholarly journals An Improved liquid Culture System for Efficient Shoot Multiplication in Aerva lanata (L.) Juss. Ex Schult.

2021 ◽  
Vol 31 (1) ◽  
pp. 35-42
Author(s):  
Kandhan Varutharaju ◽  
Chandrasekaran Thilip ◽  
Palusamy Raja ◽  
Ganesan Thiagu ◽  
Abubakker Aslam ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Solid Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiplication Rate ◽  
Mass Propagation ◽  
Liquid Culture System ◽  
Aerva Lanata ◽  
Better Than

An improved in vitro mass propagation protocol was developed for Aerva lanata using MS liquid medium. The influence of MS medium (solid and liquid) with cytokinin (TDZ and BAP, respectively) were studied for shoot proliferation and growth. The liquid medium perfomed better than solid medium in shoot multiplication. The maximum shoot multiplication rate was (29.37 ± 0.64 shoots per explant), obtained in MS liquid medium which is containing 0.6 mg/l TDZ, 0.3 mg/l NAA and 0.2 mg/l IBA. Different volumes of liquid medium have been used, 30 ml of medium flask showed the maximum number of shoots. Liquid medium is better suited for in vitro propagation of A. lanata since the enhanced multiplication rate was observed with shorter subculture intervals. Plant Tissue Cult. & Biotech. 31(1): 35-42, 2021 (June)

scholarly journals Perbanyakan Massal Embrio Kalamondin Melalui Teknologi Somatik Embriogenesis Menggunakan Bioreaktor

2013 ◽  
Vol 22 (1) ◽  
pp. 1
Author(s):  
Nirmala Frianti Devy ◽  
Farida Yulianti ◽  
Hardiyanto Hardiyanto
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Multiplication Rate ◽  
Mass Propagation ◽  
Liquid Media ◽  
Embryogenic Calli ◽  
Research Results ◽  
The Real ◽  
Subtropical Fruit

<p>Sejauh ini, penelitian perbanyakan somatik embriogenesis baik untuk penyediaan semaian batang bawah maupun varietas komersial jeruk menghasilkan laju multiplikasi yang relatif lambat. Kombinasi antara perbanyakan melalui metode somatik embriogenesis dengan penggunaan bioreaktor, diharapkan mampu meningkatkan laju produksi kalus embrionik menjadi planlet.  Kajian awal dilakukan menggunakan nuselus Kalamondin (Citrus mitis Blanco) sebagai sumber kalus. Kalus yang dihasilkan diinduksi dan diperbanyak menjadi kalus embrionik dan embrio dengan cara dikulturkan pada shaker (100 rpm) serta bulb bioreactor. Tujuan penelitian ini ialah membandingkan produksi embrio Kalamondin melalui teknologi somatik embriogenesis pada kultur cair menggunakan shaker dan bioreaktor. Penelitian dilakukan di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Jeruk dan Buah Subtropika, dari September 2008 sampai dengan Desember 2009.  Pada tahapan perbanyakan embrio dengan metode shaker, diperoleh bahwa rerata kemampuan kalus menghasilkan embrio dalam kultur selama 10 minggu ialah 18,12 embrio/g kalus. Dengan kisaran waktu yang sama, total embrio yang dihasilkan 3 g kalus/300 cc media cair di dalam bioreaktor menghasilkan 46 embrio/g kalus atau setara 2,53 kali dibandingkan metode shaker. Embrio yang tumbuh pada bioreaktor dapat berkembang hampir 100% menjadi planlet. Hasil penelitian ini membuktikan bahwa aplikasi bioreaktor untuk tujuan perbanyakan massal embrio Kalamondin memiliki pengaruh yang signifikan terhadap laju multiplikasinya.</p><p>ABSTRAK</p><p>So far, research on somatic embryogenesis for rootstock and citrus commercial varieties has been faced by low multiplication rate of embryos. Combination of somatic embryogenesis method and bioreactor hypothezed can increase multiplication rate of embryos and improve regeneration of embryogenic calli to produce plantlets.  Kalamondin explants were inducted and proliferated to be embryonic calli and embryos using both shaker (100 rpm) and bulb bioreactor. The aimed of this research was to compare the production of Kalamondin embryos through somatic embryogenesis method on liquid media using shaker and bulb bioreactor. Research was conducted at Tissue Culture Laboratory of Indonesian Citrus and Subtropical Fruit Research Institute from September 2008 to December 2009.  Kalamondin nucelus as a callus source was used in this research. Results of the study indicated that the average of embryos production through shaker technique within 10 weeks of culture incubation was 18.12 embryos/g callus, while application of bioreactor imrpoved embryo productivity up to  46 embryos/g calli (3 g/300 cc media). The multiplication rate using the bioreactor increased up to 2.53 fold compare to shaker method. Results of the study give the real evidence that application of biorector for in vitro mass propagation of Kalamondin embryos had high significant effect on embryo multiplication rate.</p>

Ancymidol Increases In Vitro Multiplication Rate of Hosta in Liquid Media

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 506c-506
Author(s):  
J. Adelberg ◽  
M. Kroggel
Keyword(s):  
Interactive Effect ◽  
High Rate ◽  
Interactive Effects ◽  
Growth Media ◽  
Multiplication Rate ◽  
Liquid Media ◽  
Culture Morphology ◽  
Shake Flasks ◽  
Root Inhibition

The main and interactive effects of plant growth regulators were assayed to optimize multiplication rate for Hosta in liquid media. Two varieties of Hosta were run through three 4-week subculture cycles in shake flasks. When Hosta `Blue Cadet' was grown on three concentrations of cytokinin (1, 2.25, and 5 μM BA), three concentrations of auxin (0.1, 1.0, and 10 μM IAA), and four concentrations of an anti-gibberellin growth regulator (0.0, 0.1, 0.32, and 1.0 μM ancymidol), multiplication rates improved markedly during the first two subculture cycles in liquid, (1.7 and 3.6x, respectively) before stabilizing at third subculture. BA and ancymidol both increased multiplication rate. IAA interacted antagonistically with BA. An optimal multiplication medium with 2.25 μM BA, 1.0 μM IAA, and 1.0 μM ancymidol, provided a high rate of multiplication with well-defined shoot morphology and minimal root growth. Media consisting of 1.0 μM BA, 10.0 μM IAA, and 0.32 μM ancymidol was effective in producing leafy plantlets, with root initials, ready for transfer to mist bed acclimatization. When Hosta `Stilletto' was grown on concentrations of cytokinin (1, 2.25, and 5 μM BA), auxin (0.1, 1.0, and 10 μM IAA) and ancymidol (0.0, 1.0, and 3.2 μM), multiplication rates improved markedly during the first two subculture cycles in liquid (1.9 and 2.4x, respectively) before stabilizing at third subculture. Ancymidol increased multiplication rate at either level; the interactive effect with BA showed highest multiplication rates at lowest BA levels in the presence of ancymidol. Lower BA concentrations promoted better culture morphology and lesser BA induced root inhibition.

scholarly journals The effects of different strength of MS media in solid and liquid media on in vitro growth of Typhonium flagelliforme

2017 ◽  
Vol 7 (2) ◽  
pp. 151-156 ◽  
Author(s):  
Nur Inani Rezali ◽  
Norrizah Jaafar Sidik ◽  
Azani Saleh ◽  
Nurul Izzati Osman ◽  
Nurul Athirah Mohd Adam
Keyword(s):  
In Vitro Growth ◽  
Liquid Media

scholarly journals IMPACT OF GROWTH REGULATORS ON IN VITRO GROWTH OF BANANA (MUSA SPP) CULTURED: A REVIEW

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Katkam Priyanka
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Multiplication Rate ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Musa Spp ◽  
Micro Propagation

Banana is important fruit crop in horticulture where it propagates through suckers which takes immense time for production and with low multiplication rate, to overcome these type of situation some protocols have been made such as the micro propagation where the tissue culture of banana is applied with help of plant growth regulators such as auxins and cytokinins are used at different concentration to attain the definite good results till now many studies had been done in the tissue culture of banana. In the present study it was observed that the explants cultured in MS medium containing 4 mg/l BAP + 0.5 mg/l IAA had highest number of shoot buds and number of shoots. Similar result was studied by Muhammad et al. (2007) where the highest multiplication ratio was observed at 4 mg/l BAP along with 1 mg/l IAA. Habiba et al. (2002) and Ahmed et al. (2014) reported that 4 mg/l BAP in combination with 2 mg/l IAA shown remarkable results.

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