Incurred sample reanalysis comparison of dried blood spots and plasma samples on the measurement of lopinavir in clinical samples

Bioanalysis ◽  
2012 ◽  
Vol 4 (3) ◽  
pp. 237-240 ◽  
Author(s):  
Roland JW Meesters ◽  
Gero P Hooff ◽  
Rob Gruters ◽  
Jeroen JA van Kampen ◽  
Theo M Luider
Keyword(s):  
Dried Blood Spots ◽  
Clinical Samples ◽  
Plasma Samples ◽  
Blood Spots ◽  
Incurred Sample Reanalysis

scholarly journals Assessment of Plasmodium antigens and CRP in dried blood spots with multiplex malaria array

2021 ◽  
Author(s):  
Ihn Kyung Jang ◽  
Sara Aranda ◽  
Rebecca Barney ◽  
Andrew Rashid ◽  
Muhammad Helwany ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Whole Blood ◽  
Dried Blood Spots ◽  
Careful Consideration ◽  
Clinical Samples ◽  
Sample Type ◽  
Sample Collection ◽  
Malaria Surveillance ◽  
Inflammatory Biomarker ◽  
Blood Spots

AbstractDried blood spots (DBS) typically prepared on filter papers are an ideal sample type for malaria surveillance by offering easy and cost-effective methods in terms of sample collection, storage, and transport. The objective of this study was to evaluate the applicability of DBS with a commercial multiplex malaria assay, developed to concurrently measure Plasmodium antigens, histidine-rich protein 2 (HRP2), Plasmodium lactate dehydrogenase (pLDH), and a host inflammatory biomarker, C-reactive protein (CRP), in whole blood. The assay conditions were optimized for DBS, and thermal stability for measurement of Plasmodium antigens and CRP in dried blood were determined. Performance of the multiplex assay on matched DBS and whole blood pellet samples was also evaluated using the clinical samples. The results indicate the acceptable performance in multiplex antigen detection using DBS samples. At cutoff levels for DBS, with a diagnostic specificity with a lower 95% confidence bound > 92%, diagnostic sensitivities against polymerase chain reaction (PCR)–confirmed malaria for HRP2, Pf LDH, Pv LDH, and Pan LDH were 93.5%, 80.4%, 21.3%, and 55.6%, respectively. The half-life of pLDH was significantly less than that of HRP2 in thermal stability studies. Results with DBS samples collected from Peru indicate that the uncontrolled storage conditions of DBS can result in inaccurate reporting for infection with P. falciparum parasites with hrp2/3 deletions. With careful consideration that minimizing the unfavorable DBS storage environment is essential for ensuring integrity of heat-labile Plasmodium antigens, DBS samples can be used as an alternative to liquid whole blood to detect P. falciparum with hrp2/3 deletions in malaria surveillance.

Measurement of Theophylline in Dried Blood Spots by Spectrophotometric Enzyme Immunoassay

1988 ◽  
Vol 25 (6) ◽  
pp. 650-653 ◽  
Author(s):  
J M Rattenbury ◽  
Tracy Taylor
Keyword(s):  
Enzyme Immunoassay ◽  
Dried Blood Spots ◽  
Plasma Samples ◽  
Blood Spots

A widely-used enzyme immunoassay for the measurement of theophylline in plasma (EMIT) has been adapted for use with dried blood spots. Potential interference from haemoglobin in eluates of the spots was avoided by autoclaving them prior to analysis. The method was investigated in terms of the recovery of theophylline added to samples, precision, and the correlation of results in DBS with those in plasma samples. The recoveries of some other drugs from eluates of autoclaved DBS were also investigated.

scholarly journals Development and validation of a LC-MS/MS method for ivermectin quantification in dried blood spots: application to a pharmacokinetic study inTrichuris trichiura-infected adults

2018 ◽  
Vol 10 (24) ◽  
pp. 2901-2909 ◽  
Author(s):  
Jessica D. Schulz ◽  
Anna Neodo ◽  
Jean T. Coulibaly ◽  
Jennifer Keiser
Keyword(s):  
Pharmacokinetic Study ◽  
Dried Blood Spots ◽  
Trichuris Trichiura ◽  
Dried Blood Spot ◽  
Plasma Samples ◽  
Blood Spots ◽  
Development And Validation ◽  
Blood Spot

Ivermectin was quantified in dried blood spot and plasma samples derived fromTrichuris trichiura-infected adults with a validated LC-MS/MS method.

Chromatographic analysis of serotonin, 5-hydroxyindolacetic acid and homovanillic acid in dried blood spots and platelet poor and rich plasma samples

2010 ◽  
Vol 1217 (29) ◽  
pp. 4808-4814 ◽  
Author(s):  
Maria Addolorata Saracino ◽  
Gilberto Gerra ◽  
Lorenzo Somaini ◽  
Marco Colombati ◽  
Maria Augusta Raggi
Keyword(s):  
Chromatographic Analysis ◽  
Homovanillic Acid ◽  
Dried Blood Spots ◽  
Plasma Samples ◽  
Blood Spots

Multiplex Analysis of 230 Medications and 30 Illicit Compounds in Dried Blood Spots and Urine

2020 ◽  
Author(s):  
Christian Tagwerker ◽  
Irfan Baig ◽  
Eric J Brunson ◽  
Davan Dutra-Smith ◽  
Mary-Jane Carias ◽  
...  
Keyword(s):  
Medication Reconciliation ◽  
Dried Blood Spots ◽  
Mass Spectrometry Analysis ◽  
Clinical Samples ◽  
Medication List ◽  
Predictive Values ◽  
Multiplex Analysis ◽  
Blood Spots ◽  
Positive Results ◽  
Urine Specimens

Abstract Drugs of abuse and medication reconciliation testing can benefit from analysis methods capable of detecting a broader range of drug classes and analytes. Mass spectrometry analysis of a wide variety of commonly prescribed medications and over-the-counter drugs per sample also allows for application of a drug–drug interaction (DDI) algorithm to detect adverse drug reactions. In order to prevent adulteration of commonly collected clinical samples such as urine, dried blood spots (DBS) present a reliable alternative. A novel method is described for qualitative and quantitative multiplex analysis of 230 parent drugs, 30 illicit drugs and 43 confirmatory metabolites by HPLC–MS-MS This method is applicable to DBS specimens collected by volumetric absorptive microsamplers and confirmable in urine specimens. A patient cohort (n = 67) providing simultaneous urine specimens and DBS resulted in 100% positive predictive values of medications or illicits confirmed by detection of a parent drug and/or its metabolite during routine medication adherence analysis. An additional 5,508 DBS specimens screened (n = 5,575) showed 5,428 (97%) with an inconsistent positive compared to the provided medication list (including caffeine, cotinine or ethanol metabolites), 29 (0.5%) with no medication list and no unexpected positive results (consistent negative) and 22 (0.4%) showed all positive results matching the provided medication list (consistent positive). A DDI algorithm applied to all positive results revealed 17% with serious and 56% with moderate DDI warnings. Comprehensive DBS analysis proves a reliable alternative to urine drug testing for extended medication reconciliation, with the added advantage of detecting DDIs.

scholarly journals Use of Dried Blood Spots to Elucidate Full-Length Transmitted/Founder HIV-1 Genomes

2016 ◽  
Vol 1 (1) ◽  
pp. 129 ◽  
Author(s):  
Jesus F. Salazar-Gonzalez ◽  
Maria G. Salazar ◽  
Damien C. Tully ◽  
Colin B. Ogilvie ◽  
Gerald H. Learn ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Full Length ◽  
Cold Chain ◽  
Clinical Samples ◽  
Envelope Gene ◽  
Acid Extraction ◽  
Resource Limited Settings ◽  
Blood Spots ◽  
Resource Limited ◽  
Hiv 1

Background: Identification of HIV-1 genomes responsible for establishing clinical infection in newly infected individuals is fundamental to prevention and pathogenesis research. Processing, storage, and transportation of the clinical samples required to perform these virologic assays in resource-limited settings requires challenging venipuncture and cold chain logistics. Here, we validate the use of dried-blood spots (DBS) as a simple and convenient alternative to collecting and storing frozen plasma.Methods: We performed parallel nucleic acid extraction, single genome amplification (SGA), next generation sequencing (NGS), and phylogenetic analyses on plasma and DBS.Results: We demonstrated the capacity to extract viral RNA from DBS and perform SGA to infer the complete nucleotide sequence of the transmitted/founder (TF) HIV-1 envelope gene and full-length genome in two acutely infected individuals. Using both SGA and NGS methodologies, we showed that sequences generated from DBS and plasma display comparable phylogenetic patterns in both acute and chronic infection. SGA was successful on samples with a range of plasma viremia, including samples as low as 1,700 copies/ml and an estimated ~50 viral copies per blood spot. Further, we demonstrated reproducible efficiency in gp160 env sequencing in DBS stored at ambient temperature for up to three weeks or at -20ºC for up to five months.Conclusions: These findings support the use of DBS as a practical and cost-effective alternative to frozen plasma for clinical trials and translational research conducted in resource-limited settings.

scholarly journals Validation of a combined ELISA to detect IgG, IgA and IgM antibody responses to SARS-CoV-2 in mild or moderate non-hospitalised patients

2020 ◽  
Author(s):  
A M Cook ◽  
S E Faustini ◽  
L J Williams ◽  
A F Cunningham ◽  
M T Drayson ◽  
...  
Keyword(s):  
Dried Blood Spots ◽  
Antibody Responses ◽  
Clinical Samples ◽  
Serum Samples ◽  
Blood Spots ◽  
Moderate Disease ◽  
Hospitalised Patients ◽  
Supplementary Material ◽  
Human Viruses ◽  
Targeted Detection

AbstractBackgroundFrequently SARS-CoV-2 results in mild or moderate disease with potentially lower concentrations of antibodies compared to those that are hospitalised. Here, we validated an ELISA using SARS-CoV-2 trimeric spike glycoprotein, with targeted detection of IgG, IgA and IgM (IgGAM) using serum and dried blood spots (DBS) from adults with mild or moderate disease.MethodsTargeting the SARS-CoV-2 trimeric spike, a combined anti-IgG, IgA and IgM serology ELISA assay was developed using 62 PCR-confirmed non-hospitalised, mild or moderate COVID-19 samples, ≥14 days post symptom onset and 624 COVID-19 negative samples. The assay was validated using 73 PCR-confirmed non-hospitalised COVID-19 and 359 COVID-19 negative serum samples with an additional 81 DBSs, and further validated in 226 PCR-confirmed non-hospitalised COVID-19 and 426 COVID-19 negative clinical samples.ResultsA sensitivity and specificity of 98.6% (95% CI, 92.6–100.0), 98.3% (95% CI, 96.4–99.4), respectively, was observed following validation of the SARS-CoV-2 ELISA. No cross-reactivities with endemic coronaviruses or other human viruses were observed, and no change in results were recorded for interfering substances. The assay was stable at temperature extremes and components were stable for 15 days once opened. A matrix comparison showed DBS to correlate with serum results. Clinical validation of the assay reported a sensitivity of 94.7% (95% CI, 90.9-97.2%) and a specificity of 98.4% (95% CI, 96.6-99.3%).ConclusionsThe human anti-IgGAM SARS-CoV-2 ELISA provides accurate and sensitive detection of SARS-CoV-2 antibodies in non-hospitalised adults with mild or moderate disease. The use of dried blood spots makes the assay accessible to the wider community.Supplementary MaterialNo

scholarly journals Oral Testosterone Administration Detected by Testosterone Glucuronidation Measured in Blood Spots Dried on Filter Paper

2000 ◽  
Vol 46 (4) ◽  
pp. 515-522 ◽  
Author(s):  
Shi-Hua Peng ◽  
Jordi Segura ◽  
Magí Farré ◽  
Xavier de la Torre
Keyword(s):  
Filter Paper ◽  
Drug Testing ◽  
Dried Blood Spots ◽  
Gas Chromatography Mass Spectrometry ◽  
Specific Marker ◽  
Plasma Samples ◽  
Suitable Alternative ◽  
Testosterone Undecanoate ◽  
Blood Spots ◽  
Sports Drug Testing

Abstract Background: Blood sampling is not a common practice for sports drug testing. Our aim was to investigate whether dried blood spots on filter paper could be an alternative to plasma samples for monitoring steroid profiles in dope testing. Methods: We collected dried blood spots and plasma from six healthy Caucasian subjects after an oral 120-mg dose of testosterone undecanoate (TU). Nonconjugated testosterone, testosterone glucuronide (TG), androsterone glucuronide (AG), and etiocholanolone glucuronide (EtG) were measured by gas chromatography–mass spectrometry in both matrices. 17α-Hydroxyprogesterone (17αOHP) and luteinizing hormone (LH) also were measured in the plasma samples. For comparison, similar measurements were done on samples obtained from the same subjects given 25 mg of testosterone propionate (TP) plus 110 mg of testosterone enanthate (TE) intramuscularly after a wash-out period. Results: After oral TU intake, TG, AG, and EtG increased sharply, whereas nonconjugated testosterone did not change significantly. Results on dried blood spots correlated well with those on plasma. The TG/testosterone ratio in blood or plasma was verified to be a sensitive and specific marker (significantly increased for up to 8 h after intake; P <0.05) for oral TU intake but not for intramuscular administration of TP plus TE. Little suppression of plasma LH and 17αOHP was observed after a single oral dose of TU. One subject did not show a significant increase of blood TG after oral TU intake. Conclusions: The measurement of glucuronide conjugates in blood and plasma samples is relevant for sports drug testing when analyzing the steroid profile. Dried blood spots collected on filter paper are a suitable alternative to plasma for detecting testosterone abuse.

A Phenytoin Assay Using Dried Blood Spot Samples Suitable for Domiciliary Therapeutic Drug Monitoring

1984 ◽  
Vol 21 (6) ◽  
pp. 519-522 ◽  
Author(s):  
E J Coombes ◽  
T R Gamlen ◽  
G F Batstone ◽  
P N Leigh
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Convenient Method ◽  
Filter Paper ◽  
Drug Monitoring ◽  
Dried Blood Spots ◽  
Therapeutic Drug ◽  
Plasma Samples ◽  
Blood Spots ◽  
Blood Spot

SUMMARY. A commercially available substrate-labelled fluorescent immunoassay procedure has been modified to create a simple rapid method for the determination of capillary phenytoin concentration in dried filter paper blood spots of patients on phenytoin therapy. The specimens are collected and despatched to the laboratory by the patient, thus domiciliary monitoring of phenytoin therapy can take place. The technique was validated by comparing the phenytoin results of simultaneously collected capillary dried blood spots and conventional plasma samples. The technique offers a convenient method for overcoming many of the practical problems of monitoring phenytoin therapy in epilepsy.

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