Generation of Reactive Oxygen Species (Ros) and Pro-Inflammatory Signaling in Human Brain Cells in Primary Culture

2013 ◽  
Vol 03 (03) ◽  
Author(s):  
Walter J. Lukiw Surjyadipta Bjattacharjee
Keyword(s):  
Reactive Oxygen Species ◽  
Human Brain ◽  
Primary Culture ◽  
Reactive Oxygen ◽  
Brain Cells ◽  
Oxygen Species ◽  
Inflammatory Signaling

Pro-and Antioxidative Properties of Cortical Tissue Preparations from Human Brain Exhibiting NMDA-Receptor Characteristics

1999 ◽  
Vol 54 (5-6) ◽  
pp. 438-445
Author(s):  
Matthias Elstner ◽  
Andrea Denke ◽  
Wieland Gsell ◽  
Erich F. Elstner ◽  
Peter Riederer ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Human Brain ◽  
Reactive Oxygen ◽  
Oh Radical ◽  
Oxygen Species ◽  
Cortical Tissue ◽  
Oxidoreductase Activity ◽  
Receptor Sites ◽  
Radical Type

Abstract The effects of cortical tissue preparations (CTP) from human brain on the production of reactive oxygen species (ROS) has been investigated with several biochemical model reactions. As indicators for ROS, fragmentation of the methionine derivatives, α-keto-γ-methyl-thiobutyric acid (KMB) or 1-amino-cyclopropane-1-carboxylic acid (ACC), yielding ethene have been used. With these systems we have shown that production of OH-radical-type oxidants by the xanthine oxidase (XOD)-system is strongly stimulated by CTP. This activity is due to intrinsic iron ions since ethene formation from KMB is stimulated by EDTA , inhibited by desferrioxamine (DesferalR) and also visible with heat-denatured CTP. CTP by themselves have no XOD activity. 3-Hydroxykynurenine (3HK) is another possible substrate for XOD but produces H2O2 without XOD-catalysis, whereas allopurinol is not inhibiting. CTP contain measurable NAD(P)H oxidoreductase activity, producing OH-radical-type oxidants at the expense of NADPH and (to a lesser extent) NADH as electron donors , shown as redox-cycling of 2-methyl-5-hydroxy-1.4-naphthoquinone, plumbagin. Ethene formation from KMB is also driven by both morpholinosydnonimine (SIN) or ONOOH. The reaction driven by SIN is stimulated by CTP and inhibited by catalase, SOD and hemoglobin. Since ethene release from KMB driven by ONOOH is inhibited by CTP the mechanisms driving KMB fragmentation are different for SIN and ONOOH. Furthermore CTP contain approx. 4 U catalase activity per mg protein and very weak peroxidase (POD) activity shown as ACC fragmentation yielding ethene in the presence of both H2O2 and KBr or NaCl. Since ACC binds to CTP and both compounds, ACC and KMB are natural products, present in food (ACC) or synthesized from methionine in vivo (KMB), these compounds may represent protecting agents in systems where reactive oxygen species are formed. One might even speculate that the production of ethene at these membrane receptor sites may have biological functions, since ethene is known to possess anaesthetic activities.

scholarly journals Lactobacillus rhamnosus blocks inflammatory signaling in vivo via reactive oxygen species generation

2009 ◽  
Vol 47 (8) ◽  
pp. 1205-1211 ◽  
Author(s):  
Patricia W. Lin ◽  
Loren E.S. Myers ◽  
Laurie Ray ◽  
Shuh-Chyung Song ◽  
Tala R. Nasr ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen Species Generation ◽  
Lactobacillus Rhamnosus ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Inflammatory Signaling

P3-359: Mitochondrial reactive oxygen species preconditioning protects brain cells against hyperglycemia

2010 ◽  
Vol 6 ◽  
pp. S557-S557
Author(s):  
Sónia C. Correia ◽  
Renato X. Santos ◽  
Maria S Santos ◽  
Paula I. Moreira
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Brain Cells ◽  
Oxygen Species ◽  
Mitochondrial Reactive Oxygen Species

scholarly journals Reactive Oxygen Species Are Involved in the Development of Gastric Cancer and Gastric Cancer-Related Depression through ABL1-Mediated Inflammation Signaling Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Tianhe Huang ◽  
Fuling Zhou ◽  
Xiaohan Yuan ◽  
Tian Yang ◽  
Xuan Liang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Reactive Oxygen Species ◽  
Cell Lines ◽  
Reactive Oxygen ◽  
The Cancer Genome Atlas ◽  
Mild Stress ◽  
Oxygen Species ◽  
Mice Model ◽  
Inflammatory Signaling

Background. The mechanisms of crosstalk between depression and gastric cancer (GC) remain ill defined. Given that reactive oxygen species (ROS) is involved in the pathophysiology of both GC and depression, we try to explore the activities of ROS in the development of GC and GC-related depression. Methods. 110 patients with newly diagnosed GC were recruited in our study. The clinical characteristics of these patients were recorded. Inflammation and oxidative stress markers were detected by ELISA. The depression status of patients with GC was assessed during follow-up. The association between ROS, ABL1, and inflammation factors was evaluated in H2O2-treated GC cell lines and The Cancer Genome Atlas (TCGA) database. The effect of ABL1 on inflammation was detected with Imatinib/Nilotinib-treated GC cell lines. A chronic mild stress- (CMS-) induced patient-derived xenograft (PDX) mice model was established to assess the crosstalk between depression and GC. Results. Depression was correlated with poor prognosis of patients with GC. GC patients with depression were under a high level of oxidative status as well as dysregulated inflammation. In the CMS-induced GC PDX mice model, CMS could facilitate the development of GC. Additionally, tumor bearing could induce depressive-like behaviors of mice. With the treatment of ROS, the activities of ABL1 and inflammatory signaling were enhanced both in vitro and in vivo, and blocking the activities of ABL1 inhibited inflammatory signaling. Conclusions. ROS-activated ABL1 mediates inflammation through regulating NF-κB1 and STAT3, which subsequently leads to the development of GC and GC-related depression.

Glucocorticoid receptor agonist compound K regulates dectin-1-dependent inflammatory signaling through inhibition of reactive oxygen species

Life Sciences ◽  
2009 ◽  
Vol 85 (17-18) ◽  
pp. 625-633 ◽  
Author(s):  
Trinh Tat Cuong ◽  
Chul-Su Yang ◽  
Jae-Min Yuk ◽  
Hye-Mi Lee ◽  
Sung-Ryong Ko ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Glucocorticoid Receptor ◽  
Receptor Agonist ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Compound K ◽  
Inflammatory Signaling

scholarly journals Reactive Oxygen Species in Metabolic and Inflammatory Signaling

2018 ◽  
Vol 122 (6) ◽  
pp. 877-902 ◽  
Author(s):  
Steven J. Forrester ◽  
Daniel S. Kikuchi ◽  
Marina S. Hernandes ◽  
Qian Xu ◽  
Kathy K. Griendling
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Inflammatory Signaling

scholarly journals The extracellular reactive oxygen species levels in primary in vitro culture of human ovarian granulosa and cumulus cells

2020 ◽  
Vol 8 (3) ◽  
pp. 112-117
Author(s):  
Ievgeniia Kocherova ◽  
Rut Bryl ◽  
Igor Crha ◽  
Pavel Ventruba ◽  
Jana Zakova ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Primary Culture ◽  
Atmospheric Oxygen ◽  
Ovarian Follicle ◽  
Reactive Oxygen ◽  
Cumulus Cells ◽  
Oxygen Metabolism ◽  
Female Reproductive Tract ◽  
Oxygen Species

AbstractIn the female reproductive tract, reactive oxygen species (ROS) may exert physiological and pathophysio-logical effects. Although ROS play an essential role as the signaling molecules, their excessive accumulation contributes to the pathogenesis of many reproductive processes. In the ovarian follicle, ROS affect multiple physiological processes, including oocyte maturation and fertilization. However, a lack of studies showing to which extend ovarian granulosa and cumulus cells can contribute to the development of oxidative stress within the ovarian follicle. In the presented research, the extracellular ROS accumulation level was investigated using GCs and CCs primary in vitro cultures. The obtained results demonstrated a steady decrease in extracellular ROS level during GCs primary culture. By contrast, ROS concentration in CCs conditioned medium increased gradually between the first and the seventh days of culture. The observed changes may reflect the proliferation status and metabolic activity of GCs and CCs during in vitro culture. Additionally, the elevated ROS level at respective points of time could occur as a consequence of culture in atmospheric oxygen. The distinct function and localization within the ovarian follicle may explain the differences between GCs and CCs oxygen metabolism.Running title: Reactive oxygen species in primary culture of human follicular cells

scholarly journals Pecularities of reactive oxygen species accumulation in the primary culture of nerve cells of Lymnaea stagnalis

2021 ◽  
pp. 28-38
Author(s):  
Valentina R. Haritonova ◽  
Alexander V. Sidorov
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Primary Culture ◽  
Lymnaea Stagnalis ◽  
Unit Area ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Intracellular Space ◽  
The Neural Network ◽  
Neuronal Functions

A fluorescent dye 2′,7′-dichlorodihydrofluorescein diacetate was used to characterize an accumulation of reactive oxygen species (ROS) in the primary culture (72 and 144 h) of neurons of the central ganglia of the mollusc Lymnaea stagnalis under conditions of acute, induced oxidative stress. It was found that larger cells accumulate a greater amount of ROS. High values of the relative (per unit area) fluorescence, indicating an increase in the amount of ROS per unit of intracellular space, are characteristic of smaller neurons of 72-hour culture. Changes in relative fluorescence over time are different from each other in neurons of 72- and 144-hour cultures. For an older culture, a negative, statistically significant relationship was noted (R = – 0.31), and for cells of 72 h of cultivation, a statistically significant correlation was not found (R = 0.12). It is assumed that the discovered relationship between the size (shape) of a neuron and its ability to resist the accumulation of ROS in the cytosol indicates differences in the resistance of CNS neurons to oxidative damage depending on their place and position in the neural network, thereby ensuring the selective stability of neuronal functions under conditions of oxidative stress.

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