Isolation and Identification of Saprolegnia Sp from Fresh Water Aquarium Fishes and the Hemolymph Immune Response of Dactylopus coccus Costa de 1835 (Homoptera: Coccoidea: Dactylopidae) against this Oomycete

The research work was performed for the isolation and identification of Pasteurella multocida from field cases, preparation of oil adjuvanted vaccine from isolated strain and determination of its efficacy. Samples were collected from suspected dead birds of three poultry farms of Bangladesh (Code name: M and R). The P. multocida isolates were Gram negative, non-motile, non- spore forming rod occurring singly or pairs and occasionally as chains or filaments. Biochemically P. multocida ferment basic sugar and consistently produced acid except from maltose and lactose. After isolation formalin killed oil adjuvanted Fowl cholera vaccine was prepared in Laboratory of the Department of Microbiology and Hygiene, BAU and this experimental vaccine (3.2x108 CFU/ml) was administered in nine weeks old White Leg Horn chickens at the different dose rate through intramuscular (IM) route in each selected group A (1ml alum precipitated vaccine), B (0.5ml alum precipitated vaccine), C (1ml oil adjuvanted vaccine) and D (0.5ml oil adjuvanted vaccine). Pre-vaccinated sera were collected from all groups of birds. The mean of Passive Hemagglutination (PHA) titers of post-vaccination were 51±17.8, 76.8±17, 89.6±17, and 115±17.81 in group A, B, C and D respectively which consist of 5 birds in each. The vaccine produced better immune response when boostering with the similar dose and route at 15 days after primary vaccination. The mean PHA titers were higher at group D than other groups after boostering. Challenge infection was conducted on all the vaccinated and control group (n=5) of birds after 15 days of vaccination which protect 93.75% of birds and the PHA titers from different groups analyzed to determine the protective capacity of vaccinated chickens against challenge exposure. It was demonstrated that experimental oil adjuvanted fowl cholera vaccine with 0.5ml dose produce higher immune response against challenge infection and found to be safe. Microbes and Health, June 2013, 2(1): 1-4DOI: http://dx.doi.org/10.3329/mh.v2i1.17253

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Isolation and Identification of Some Probiotic Bacteria and Their Potential Role in Improving Immune Response and Resistance of Nile Tilapia (Oreochromis niloticus) in Comparison with a Commercial Product

International Journal of Microbiology ◽

10.1155/2020/8865456 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Mosleh M. Abomughaid

Keyword(s):

Immune Response ◽

Oreochromis Niloticus ◽

Nile Tilapia ◽

Management Tool ◽

Phagocytic Index ◽

Control Group ◽

Second Phase ◽

Feeding Experiment ◽

Isolation And Identification

This work aimed to retrieve a field isolate of probiotic from Nile tilapia (Oreochromis niloticus) and compare the obtained results with a commercial probiotic product through experimental studies. The study was conducted on 250 Nile tilapia. Ten fish were used to isolate the probiotic strain. Two isolates showed an in vitro inhibitory effect against pathogenic A. hydrophila. The isolate with the largest zone was identified by PCR. Sixty fish were used to test the safety of a potential probiotic. One hundred and eighty fish were used in a two-month feeding experiment. Fish were divided into 3 groups, group (1): the control, group (2): fed on potential probiotics, and group (3): fed on commercial probiotic (Organic Green™). The effects of tested products on the immune response were recorded in all groups. After one and two months of feeding experiment, blood and nonspecific immune parameters were evaluated. Disease resistance against Aeromonas hydrophila was evaluated through challenge experiment. The histopathology of the treated groups was fully recorded in comparison with the control group. The potential probiotic based on the in vitro antimicrobial activity test was identified as P. putida using routine and gel electrophoresis and 16S rRNA sequencing. During the first and the second month of experiment, there was a highly significant increase in the survival percent of the experimental fish in both treated groups with probiotics. In the first phase of the experiment, a significant increase in the haematocrit values and NBT, lysozyme activity, and phagocytic activity was seen in all treated groups in comparison with the control. The increase in the TLC was significant in the group fed with P. putida in comparison with the control group. In the second phase, a nonsignificant increase in the hematocrit values and significant increases in the NBT and phagocytic index were seen in P. putida and organic green groups in comparison with the control group. The TLC and DLC revealed nonsignificant changes in the treated groups in comparison with the control. The RLP in the groups treated with P. putida was higher than that in those treated with organic green. Although probiotics are an important management tool in aquaculture, it should be subjected to scientific laboratory tests and field measurements.

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Isolation And Identification Of Some Chemical Constituents In Two Different Types Of Fresh Water Macro Algae In Bestansur Village In Suleiman City Kurdistan Region (North Iraq) By Hplc Technique

IOSR Journal of Applied Chemistry ◽

10.9790/5736-0434555 ◽

2013 ◽

Vol 4 (3) ◽

pp. 45-55 ◽

Cited By ~ 2

Author(s):

Trifa Attar Omer Trifa Attar Omer

Keyword(s):

Fresh Water ◽

Chemical Constituents ◽

Kurdistan Region ◽

Isolation And Identification ◽

Different Types ◽

North Iraq ◽

Hplc Technique ◽

Macro Algae

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Toll-Like Receptors (TLRs) Play Role in Adaptive Immunity in Rabbits Immunized by Sarcoptes scabiei Proteins

KnE Life Sciences ◽

10.18502/kls.v3i6.1100 ◽

2017 ◽

Vol 3 (6) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

Nunuk Dyah Retno Lastuti ◽

Fedik Abdul Rantam ◽

Poedji Hastutiek ◽

Dony Chrismanto

Keyword(s):

Immune Response ◽

Innate Immunity ◽

Adaptive Immunity ◽

Cellular Immune Response ◽

Sarcoptes Scabiei ◽

Toll Like Receptors ◽

Isolation And Identification ◽

Ssrna Virus ◽

Significant Difference ◽

Cellular Immune

Sarcoptic mange is one of the most economically important diseases in goats in Indonesia, and increasing number of cases of treatment failure is being reported because of drug resistance. Nowadays, it is considered as an emerging/re-emerging parasitic disease that threatens human and animal health globally. Toll-Like Receptors (TLR) is a receptor which plays role in innate immunity due to microbial infection. TLR-5 is a receptor that can recognize ligand produced by bacterial component, and TLR-7 is involved in the recognition of ligand that similar to ssRNA virus. This research aims are to detect Sarcoptes scabiei antigenic protein which can induce cellular immune response in rabbit as adaptive immunity with TLR-5 and TLR-7 as marker.This research was performed in several stages: isolation and identification S. scabiei from scabies infected goats; extraction of soluble protein S.scabiei mite, the rabbit immunization by inoculating protein antigen S. scabiei with dosage of 500µg, repeated five times as booster in two weeks, the examination of TLR-5 and TLR-7 expression using direct immunofluorescence technique. The result of cellular immune response is shown by TLR-5 and TLR-7 expression in rabbit T limphocytes which appear yellow to green fluorescence color using fluorescence microscope. The amount of fluoresnce T lymphocytes showed a significant difference (p<0.05) between control and various boosters, and significantly increased in 3rd booster or 42 days post immunization. The conclusion showed protein from S. scabiei mites taken from goat contains ligands, acting as receptors which involve in pathogen associated molecular pattern (PAMP) that can induce adaptive immunity and recognized by TLR-5 and TLR-7. It shows that TLR is not only involved in innate immunity but also in adaptive immunity, and can be used as alternative adjuvant development. Keywords: Sarcoptes scabiei, TLR- 5, TLR-7, goats, rabbits.

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