ISOLATION AND IDENTIFICATION OF STAPHYLOCOCCUS BACTERIA FROM FISH OF FRESH WATER AND ITS ANTIBIOTICS SENSITIVITY IN MOSUL CITY

2014 ◽  
Vol 13 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Haifaa Hussein Ali
Keyword(s):  
Fresh Water ◽  
Isolation And Identification ◽  
Antibiotics Sensitivity

scholarly journals First Detection of Pathogenic Escherichia coli Isolates Associated With Donkey Foals’ Diarrhea in Northern China

2020 ◽  
Author(s):  
Liu Wen-qiang ◽  
Xia Nan ◽  
Zhang Jing-wen ◽  
Wang Ren-hu ◽  
Jiang Gui-miao
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Virulence Factors ◽  
Influence Factor ◽  
Genetic Relationships ◽  
Northern China ◽  
Multi Drug Resistance ◽  
Rrna Gene ◽  
Isolation And Identification ◽  
Antibiotics Sensitivity

ABSTRACTObjectiveThe aim of this study was to identify the biological features, influence factor and Genome-wide properties of pathogenic donkey Escherichia coli (DEC) isolates associated with severe diarrhea in Northern China.MethodsThe isolation and identification of DEC isolates were carried out by the conventional isolation、automatic biochemical analysis system、serotype identification、16S rRNA test、animal challenge and antibiotics sensitivity examination. The main virulence factors were identified by PCR. The complete genomic re-sequence and frame-sequence were analyzed.Results216 strains of DEC were isolated from diarrhea samples, conforming to the bacterial morphology and biochemical characteristics of E.coli. The average size of the pure culture was 329.4 nm×223.5 nm. Agglutination test showed that O78 (117/179, 65.4%) was the dominant serotype and ETEC(130/216, 60.1%) was the dominant pathogenic type. Noticeable pathogenic were observed in 9 of 10 (90%) randomly selected DEC isolates caused the death of test mice (100%, 5/5) within 6h∼48h, 1 of 10 (10%) isolates caused the death of test mice (40%, 2/5) within 72h. Our data confirmed that DEC plays an etiology role in dirarrea/death case of donkey foal. Antibiotics sensitivity test showed significant susceptibility to DEC isolates were concentrated in Nor、EFT、ENR、CIP and AMK,while the isolates with severe antibiotic resistance was AM、TE、APR、FFC、RL and CN. Multi-drug resistance was also observed. A total of 15 virulence gene fragments were determined from DEC(n=30) including OMPA (73%), safD (77%), traTa (73%), STa(67%), EAST1 (67%), astA (63%), kspII (60%), irp2 (73%), iucD (57%), eaeA (57%), VAT (47%), iss (33%), cva (27%), ETT2 (73%) and K88 (60%) respectively. More than 10 virulence genes from 9 of 30(30%) DEC strains were detected, while 6 of 30(20%) DEC strains detected 6 virulence factors. phylogenetic evolutionary tree of 16S rRNA gene from different isolates shows some variability. The original data volume obtained from the genome re-sequencing of DEC La18 was 2.55G and Genome framework sequencing was carried out to demonstrate the predicted functions and evolutionary direction and genetic relationships with other animal E.coli.ConclusionsThese findings provide firstly fundamental data that might be useful in further study of the role of DEC and provide a new understanding of the hazards of traditional colibacillosis due to the appear of new production models.

scholarly journals Detection of Antibiotics Sensitivity at Different PH Levels for Proteus Mirabilis Isolated from Patients with Urinary Tract Infections

2018 ◽  
Vol 26 (5) ◽  
pp. 132-144
Author(s):  
Ehsan F. Hussein
Keyword(s):  
High Activity ◽  
Proteus Mirabilis ◽  
Penicillin G ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Neutral Ph ◽  
Antibiotics Susceptibility ◽  
Antibiotics Sensitivity ◽  
Tract Infections

One hundred and sixty-nine samples of urine have been collected through the period from December/2016 to May/2017. Were for isolation and identification of Proteus mirabilis. Isolated from urine in patients with UTI. Identification was done by grow-  ing on different media and biochemical tests as well as the antibiotics susceptibility were studied by using twenty types of antibiotics at acidic pH, neutral pH and alkaline or basic pH by disc diffusion method. These antibiotics were involved Ciprofloxacin, Amikcin, Meromenem, Imipenem, Ampicillin, Rifampin, Gentamicin, Trimethoprim, Tetracycline, Amoxicillin, Sulfamethoxazole, Carbnicillin, Rifaximin, Penicillin-G, Ox- olinic acid, Bacitracin, Clindamycin, Erthromycin, Novamicin and Aztreomycin. The Ciprofloxacin and Aztreomycin have high activity at both acidic and neutral pH, while the Amikcin, Meromenem, Imipenem and Ciprofloxacin have high activity at basicity pH against these bacteria.

scholarly journals Retail chicken meats as potential sources of Clostridioides difficile in Al-Jouf, Saudi Arabia

2021 ◽  
Vol 15 (07) ◽  
pp. 972-978
Author(s):  
Ahmed Elsayed Taha Attia
Keyword(s):  
Saudi Arabia ◽  
Wide Spectrum ◽  
Human Infection ◽  
Chicken Meat ◽  
Isolation And Identification ◽  
Clostridioides Difficile ◽  
Potential Sources ◽  
Toxigenic Strains ◽  
Antibiotics Sensitivity ◽  
Meat Samples

Introduction: Presence of Clostridioides difficile in stool of food birds and animals is a risk for contamination of their meats to become potential sources of human infection. The main virulence factors of C. difficile are its resistance to antibiotics, production of toxins and spores. As far as I know, this is the first study to evaluate C. difficile prevalence in chicken meats, its toxigenic activities and antibiotics sensitivity patterns in Al-Jouf, Saudi Arabia. Methodology: Totally, 250 raw chicken meat samples were examined. Standard microbiological and biochemical procedures were used for C. difficile isolation and identification. The suspected colonies were tested by L-proline and C. difficile test kits then confirmed by Vitek 2 compact system. Xpect C. difficile toxin A/B test was used to detect A/B toxins production. Antibiotics susceptibility patterns were detected by Epsilon tests. Results: C. difficile was isolated from 11/250 (4.40%) chicken meat samples; 5/65 (7.69%) legs, 3/65 (4.61%) thighs, 2/60 (3.33%) wings and 1/60 (1.67%) breasts (p = 0.4). All isolates were non-toxigenic. Although all isolates were vancomycin sensitive, some isolates were intermediate/resistant to metronidazole, tetracycline, clindamycin or moxifloxacin antibiotics with variable degrees. Conclusions: C. difficile might contaminate retail chicken meats. Although low level of contamination by non-toxigenic strains was detected, chicken meats should be investigated as C. difficile infection sources for humans especially elders, immune-compromised and long terms wide spectrum antibiotics-used persons. Decreased sensitivity of C. difficile to antibiotics is emerging.

Isolation and Identification of Main Microorganisms in Different Water Systems in Northern Hangzhou, China

2014 ◽  
Vol 522-524 ◽  
pp. 445-449
Author(s):  
De Yong Zhang ◽  
Xiao Lu Xu ◽  
Yin Lu ◽  
Xiu Ying Shen ◽  
Hui Ying Xu ◽  
...  
Keyword(s):  
Sensitivity Analysis ◽  
Water Purification ◽  
Aquatic Ecosystems ◽  
Water Systems ◽  
Human Diseases ◽  
Industrial District ◽  
Isolation And Identification ◽  
Grand Canal ◽  
Antibiotics Sensitivity

Microorganisms in aquatic ecosystems play an important role in the processes like water purification, nutrition regeneration and biochemical circulation. Some microorganisms are also related to human diseases. To analyze the main microorganisms in the water systems in northern Hangzhou, water was sampled from three different rivers for isolation and identification of microorganisms. The highest diversity and quantity of bacterium was found in the rivers in Banshan industrial district, with an average 127 clones in each plate. For the Beijing-Hangzhou Grand Canal and Longshan reservoir, the number was 20 and 7, respectively. Similar trends were also observed for actinomycetes and molds. CCA analysis showed that the microorganism abundance was mostly related to the NO3-N, TP and TN values of water. Totally, 7 bacterium strains were identified, includingBacilluscereus,Escherichiacoli.,Klebsiellapneumoniae, and so on, which belong to 7 different genera. In an antibiotics sensitivity analysis, all of the 7 bacteria showed sensitivity to tetracycline.

scholarly journals Studies on glycolipids. Part VIII. Isolation and Identification of Anti-tumor-Promoting Principles from the Fresh-Water Cyanobacterium Phormidium tenue.

1993 ◽  
Vol 41 (9) ◽  
pp. 1664-1666 ◽  
Author(s):  
Hideaki SHIRAHASHI ◽  
Nobutoshi MURAKAMI ◽  
Miyako WATANABE ◽  
Akito NAGATSU ◽  
Jinsaku SAKAKIBARA ◽  
...  
Keyword(s):  
Fresh Water ◽  
Isolation And Identification

scholarly journals Isolation and Identification of bacterial isolates from ear infection and their sensitivity to usual antibiotics in human and dogs

2011 ◽  
Vol 35 (1) ◽  
pp. 159-166
Author(s):  
AL- Nassry B. S.
Keyword(s):  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Ear Infection ◽  
E Coli ◽  
Antibiotics Sensitivity ◽  
Zoonotic Bacteria ◽  
Klebsiella Spp

Bacterial examination was done on 50 dogs suffering from ear infection. Dogs examined include both sexes and their ages were between 5 months to 5 years, In the other hand 35 ear smears from male and female human in ages between 7-40 years were also taken . Isolation and identification of bacterial isolates were done to determine species of zoonotic bacteria in human and dogs, also study of antibiotics sensitivity to these isolates. We identified 137 bacterial isolates from dogs ear belonge to 9 bacterial spp. which are : S. aureus( 27.7%) , Klebsiella spp. ( 13.8%) , E. coli ( 13.1%) , Streptococcus spp. ( 12.4%) ,P. haemolytica ( 8.2%) , P. vulgaris ( 7.2%) , S. epidermidis ( 6.5%) , Salmonella spp. ( 5.8%) , P. mirabilis ( 5.1%) . In human ear smears 95 isolates were identified to 11 bacterial spp. as follows : S. aureus( 26.3%) , Streptococcus spp. ( 15.7%) , S. epidermidis ( 13.6%) , Citrobacter spp. ( 9.4%) , P. mirabilis ( 8.4%) , Klebsiella spp. ( 6.3%) , P. vulgaris ( 5.2%) , Salmonella spp. ( 5.2%) , P. aeruginosa ( 4.2%) , Shigella spp. ( 3.1%) , E. coli ( 2.1%) . Antibiotic sensitivity to dog isolates show high sensitivity to Imipenem (IPM) then Streptomycin ( S) then Rifampin ( RA) then Clindamycin ( DA) and lowest to Erythromycin ( E) respectively . but most isolates were resistant to Cefotaxime( CTX) then Ampicillin ( AM) then Vancomycin ( VA) and lowest to Trimethoprim ( TMP) respectively . The human isolates show high sensitivity to Imipenem ( IPM ) then Streptomycin ( S ) then Erythromycin ( E ) then Rifampin ( RA) then Clindamycin ( DA) finally Vancomycin ( VA) . but most isolates were resistant to Cefotaxime ( CTX ) then Ampicillin ( AM) finally Trimethoprim (TMP). P. aeruginosa was resistant to all antibiotics except Imipenem ( IPM) , Streptomycin ( S) and Erythromycin ( E)

scholarly journals IN VITRO SENSITIVITY PROFILES OF ENTERIC BACTERIA ASSOCIATED WITH DIARRHEIC PATIENTS WITHIN KATSINA METROPOLIS, KATSINA STATE, NIGERIA

2020 ◽  
Vol 4 (2) ◽  
pp. 178-182
Author(s):  
Joseph Odewade ◽  
Albert Fasogbon ◽  
Felix Onyekachi
Keyword(s):  
Escherichia Coli ◽  
Diarrheal Disease ◽  
Enteric Bacteria ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Isolation And Identification ◽  
Sensitivity Testing ◽  
Stool Samples ◽  
Antibiotics Sensitivity

Diarrheal disease is a leading cause of mortality and morbidity across the globe. However, there is little information on the prevalence and antimicrobial sensitivity patterns of microbes associated with diarrheic patients within Katsina Metropolis, Nigeria, hence, the need for this study. Forty (40) stool samples were collected from Federal Medical Center, Katsina, Katsina State, Nigeria. Isolation and identification of the bacteria from the stool samples were carried out using standard microbiological techniques. Antibiotics sensitivity testing was carried out on all the bacterial isolates using disk diffusion method. Seventeen (17) bacterial isolates were isolated from three different genera. These include: Escherichia coli (58.82%), Salmonella typhi (29.41%) and Shigella dysenteriae (11.77%). The results of antibiotics sensitivity test showed a high (100%) resistance to amoxicillin, 70.59% resistance to streptomycin, 41.18% resistance to nalixidic acid and least (17.65%) resistance to imipenem. Multi drug resistant Escherichia coli and Shigella species were also detected. The high level of antibiotic resistance among bacterial isolates obtained from stool samples of diarrheic patients is quite alarming and requires urgent public health attention. Hence, further studies are required for the molecular detection of the resistant genes in these bacteria.

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