scholarly journals Rice Salt-Tolerance Enhancement by Expression of 1-Aminocyclopropane-1- Carboxylic Acid Oxidase Gene from Salt Tolerant Barley

2018 ◽  
Vol 06 (04) ◽  
Author(s):  
Sugimoto M ◽  
Houjyo Y ◽  
Maekawa M ◽  
Terada R
2013 ◽  
pp. 337-343
Author(s):  
B.G. Defilippi ◽  
P. Muñoz-Robredo ◽  
M. Madariaga ◽  
O. Gudenschwager ◽  
M. González-Agüero

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Rogayah Sekeli ◽  
Janna Ong Abdullah ◽  
Parameswari Namasivayam ◽  
Pauziah Muda ◽  
Umi Kalsom Abu Bakar

A high survival rate for transformed papaya plants when transferred to the field is useful in the quest for improving the commercial quality traits. We report in this paper an improved rooting method for the production of transformed Malaysian Eksotika papaya with high survival rate when transferred to the field. Shoots were regenerated from embryogenic calli transformed with antisense and RNAi constructs of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) genes using the Agrobacterium tumefaciens-mediated transformation method. Regenerated transformed shoots, each measuring approximately 3-4 cm in height, were cultured in liquid half-strength Murashige and Skoog (MS) medium or sterile distilled water, and with either perlite or vermiculite supplementation. All the culturing processes were conducted either under sterile or nonsterile condition. The results showed that rooting under sterile condition was better. Shoots cultured in half-strength MS medium supplemented with vermiculite exhibited a 92.5% rooting efficiency while perlite showed 77.5%. The survival rate of the vermiculite-grown transformed papaya plantlets after transfer into soil, contained in polybags, was 94%, and the rate after transfer into the ground was 92%. Morpho-histological analyses revealed that the tap roots were more compact, which might have contributed to the high survival rates of the plantlets.


1999 ◽  
Vol 26 (2) ◽  
pp. 179 ◽  
Author(s):  
Maria X. Henzi ◽  
David L. McNeil ◽  
Mary C. Christey ◽  
Ross E. Lill

In this paper 11 transgenic broccoli (Brassica oleracea L. var. italica) lines containing a tomato antisense 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene from pTOM13 were evaluated. Changes in respiration, ethylene production and ACC oxidase activity were studied in mature flowers. Averaged across all ACC oxidase transgenic lines, there was an initial increase followed by a substantial decrease in ethylene production compared with the controls. Of the 11 transgenic lines, 10 lines showed a significant reduction in fethylene production relative to the controls from 50 h after harvest. Green Beauty flowers showed a significant reduction in respiration between the transgenics and control and demonstrated how ethylene levels could control the stable, or climacteric-like increase in respiration. ACC oxidase activity was higher in transgenic plants, consistent with the initially higher ethylene production. ACC oxidase activity did not, however, reflect the increase in ethylene production found after 50 h for the controls. These results suggest that two ethylene production systems may operate with only the second being inhibited by the antisense ACC oxidase used and that the later system was not detected by the ACC oxidase assay used. The results do show that post-harvest ethylene synthesis and therefore possibly broccoli senescence can be regulated by using an antisense ACC oxidase gene.


Plant Science ◽  
2010 ◽  
Vol 178 (3) ◽  
pp. 251-257 ◽  
Author(s):  
Shengzhu Huang ◽  
Toshiko Sawaki ◽  
Ayumi Takahashi ◽  
Shinji Mizuno ◽  
Kanako Takezawa ◽  
...  

2013 ◽  
Vol 19 (2) ◽  
pp. 57-65
Author(s):  
MH Kabir ◽  
MM Islam ◽  
SN Begum ◽  
AC Manidas

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008


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