scholarly journals Agrobacterium tumefaciens Mediated Transformation of Symbiodinium spp.

2021 ◽  
Vol 21 (06) ◽  
pp. 291-298
Author(s):  
Buntora Pasaribu ◽  
Pei-Luen Jiang
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Light Microscopy ◽  
Binary Vector ◽  
Marine Invertebrate ◽  
Gene Transformation ◽  
Free Living ◽  
Histochemical Assay ◽  
Gus Histochemical Assay ◽  
Foreign Genes

Symbiodinium spp conducts symbiosis mutualism within a wide phyletic range of marine invertebrate hosts including corals and anemones. The present study investigates the transformation of foreign genes into the free living cultured Symbiodinium spp by co-cultured Symbiodinium cells with A. tumefaciens. Ti-plasmidbased binary vector harboring the GUS and GFP genes were transformed into Symbiodinium cells by co-cultivation. GUS histochemical assay was monitored in Symbiodinium cells under light microscopy. Putative GFP in transformed Symbiodinium cells was detected by immunoassaying with antibodies against GFP protein. These results suggest that A. tumefaciens could provide efficient tools for gene transformation of Symbiodinium cells.

scholarly journals GENETIC TRANSFORMATION AND REGENERATION OF TRANSGENIC SWEETPOTATO PLANTS.

HortScience ◽  
1995 ◽  
Vol 30 (3) ◽  
pp. 435f-435 ◽  
Author(s):  
Marceline Egnin ◽  
C.S. Prakash
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Transgenic Plants ◽  
Genetic Transformation ◽  
Binary Vector ◽  
Blot Hybridization ◽  
Pcr Amplification ◽  
Ms Medium ◽  
Petiole Explants ◽  
Efficient Delivery ◽  
Foreign Genes

This study aimed to optimize factors for the efficient delivery of foreign genes into sweetpotato using Agrobacterium tumefaciens and develop transgenic plants. Disarmed Agrobacterium C58 carrying a binary vector pBI 121C2H with gusA, nptll, and the nutritional protein asp-l genes was used to cocultivate (4 days) petiole explants of the sweetpotato genotype P1318846-3. Pre-incubation of petioles for 3 days on MS medium with 2,4-D (0.2 mg·liter–1) before infection resulted in higher transformation. Putative transgenic shoots were obtained by transfer of petioles to MS medium with TDZ (0.2 mg·liter–1) and kanamycin (80 to 140 mg·liter–1). The PCR amplification of gusA, nptll, and asp-1 genes in the 37 putative transgenic shoots showed that six plants contained the three genes. However, none of these plants showed histochemical expression of the gusA gene. The introduced gene may have been methylated resulting in the lack of its expression. DNA blot hybridization studies are underway to verify the presence and integration of the transgenes.

Introduction of foreign genes into potato cultivars Bintje and D�sir�e using an Agrobacterium tumefaciens binary vector

1988 ◽  
Vol 7 (1) ◽  
pp. 47-50 ◽  
Author(s):  
Willem J. Stiekema ◽  
Freek Heidekamp ◽  
Jeanine D. Louwerse ◽  
Harrie A. Verhoeven ◽  
Paul Dijkhuis
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Binary Vector ◽  
Potato Cultivars ◽  
Foreign Genes

Ultrastructures of Epibiotic Suctorian, Tokophrya huangmeiensis 

Zootaxa ◽  
2018 ◽  
Vol 4521 (1) ◽  
pp. 145
Author(s):  
URFA BIN TAHIR ◽  
DENG QIONG ◽  
WANG ZHE ◽  
LI SEN ◽  
LIU YANG ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Structural Changes ◽  
Small Subunit ◽  
Free Living ◽  
Transmission Electron ◽  
Small Subunit Ribosomal Dna ◽  
Ribosomal Dna Sequence ◽  
Freshwater Ciliates

Tokophrya species are either free-living or facultative ectosymbiotic suctorians associated with copepods, isopods, mysids, decapods and amphipods. Tokophrya huangmeiensis in particular is found to be epizoic with the redclaw crayfish Cherax quadricarinatus Von Martens, 1868, which has been observed as part of an ongoing investigation of freshwater ciliates biodiversity in Huanggang, Hubei, China (Tahir et al. 2017). This first study on T. huangmeiensis based on morphological features using light microscopy and small subunit ribosomal DNA sequence (Tahir et al. 2017), suggested that more detailed descriptions on the physiological and structural changes of this species should be done. Thus, in this study, we looked at the ultrastructures of T. huangmeiensis using electron microscopy, including both scanning (SEM) and transmission electron microscopy (TEM). 

scholarly journals An Improved Binary Vector and Escherichia coli Strain for Agrobacterium tumefaciens-Mediated Plant Transformation

2016 ◽  
Vol 6 (7) ◽  
pp. 2195-2201 ◽  
Author(s):  
Michael R. Watson ◽  
Yu-fei Lin ◽  
Elizabeth Hollwey ◽  
Rachel E. Dodds ◽  
Peter Meyer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Agrobacterium Tumefaciens ◽  
Plant Transformation ◽  
Binary Vector ◽  
Coli Strain

GUS Gene Transformation in Rice (Oryza sativa L.) Variety BRRI Dhan-30 Mediated by Agrobacterium tumefaciens

2008 ◽  
Vol 7 (3) ◽  
pp. 530-536 ◽  
Author(s):  
M. Asaduzzama ◽  
M.A. Bari ◽  
M. Rahman ◽  
M. Minami ◽  
K. Matsushima ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Agrobacterium Tumefaciens ◽  
Oryza Sativa L ◽  
Gene Transformation ◽  
Gus Gene

scholarly journals Life cycle of Craspedacusta sowerbyi xinyangensis

2009 ◽  
Vol 55 (3) ◽  
pp. 227-234 ◽  
Author(s):  
Shanliang Xu ◽  
Danli Wang
Keyword(s):  
Life Cycle ◽  
Light Microscopy ◽  
Digital Camera ◽  
Zhejiang Province ◽  
The Other ◽  
Free Living ◽  
Cell Stage ◽  
Germ Layers ◽  
Male And Female ◽  
Gastrovascular Cavity

Abstract To explore the life cycle of Craspedacusta, the authors collected male and female specimens of the Craspedacusta sowerbyi xinyangensis in a small fire-fighting pond in Ningbo, Zhejiang Province in July, 2005 and 2006. The development of C. sowerbyi xinyangensis was studied from zygote to medusa by means of light microscopy and digital camera. The zygotes of C. sowerbyi xinyangensis are globular and smooth (90 - 105 μm diameter) and have an equal, total cleavage to the two-cell stage 15 min after fertilization. The embryos enter the four-cell stage after another 15 min and become multicellular embryos after 3h 15 min. At this stage the embryos have a diameter similar to fertilized eggs but have uneven surfaces that are distinct from the smooth surfaces of the uncleaved zygotes. Solid gastrulae are formed 7 h after fertilization. These are spherical planulae with short surface cilia that begin to swim in slow clockwise circles. After 12 h, they lose their cilia, cease swimming and become elongated planulae with one end larger than the other. Rod-like planulae, similar in thickness at both ends, are formed after an additional 7 h. After 4 days, the planulae develop into tiny polyps having two germ layers and a gastrovascular cavity. The polyp mouth is 50 - 62 µm in diameter, lacking tentacles but having nematocysts around the mouth. Planulae become mature polyps after 10 days (15 days after fertilization). Medusa buds (45 - 88 μm diameter) are formed by polyp budding, which soon become free-living medusae with 8 tentacles (380 - 620 μm diameters). Sometimes, the movement of frustules, which are formed by the polyps and similar to planulae in morphology can also be observed.

scholarly journals Agrobacterium-mediated genetic transformation of two varieties of Brassica: optimization of protocol

1970 ◽  
Vol 34 (2) ◽  
pp. 287-301 ◽  
Author(s):  
MMA Khan ◽  
ABMAHK Robin ◽  
MAN Nazim-Ud-Dowla ◽  
SK Talukder ◽  
L Hassan
Keyword(s):  
Brassica Napus ◽  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Brassica Rapa ◽  
Binary Vector ◽  
Gus Assay ◽  
Key Words Transformation ◽  
Gus Reporter ◽  
Best Response ◽  
Transformation Experiment

 Two rapeseed varieties, namely Tori-7 and BARI Sarisha-8, respectively, from Brassica rapa and Brassica napus were selected to observe the transformation ability. Petioles were inoculated in Agrobacterium tumefaciens strain LBA 4404 carrying a binary vector pBl2l with GUS (reporter) and nptII (kanamycin resistant) gene. The transformation experiment was performed by optimizing two important factors: preculture time and co-cultivation time and also selected out the best variety. Infection was most effective when explants were pre-cultured for 72 hours (80% GUS positive). and co-cultivated for 72 hours (72% GUS positive). The variety Tori-7 showed the best response to GUS assay (65% GUS positive). Callus induction was the highest in Tori-7, which were 6% with 72 hours of preculture period and 9% in 48 hours of co-cultivation. Number of putative transformed plantlets were highest in Tori-7 (7 plants) followed by BARI Sarisha-8 (3 plants). Key words: Transformation; Brassica; GUS; Agrobacterium. DOI: 10.3329/bjar.v34i2.5802Bangladesh J. Agril. Res. 34(2): 287-301, June 2009

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