scholarly journals Transcriptome Analysis of Immune Response Process in Yellow Catfish (Pelteobagrus fulvidraco) During Edwardsiella tarda Exposure

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Ying Yang ◽  
Junhao Zhou ◽  
Pengfei Miao ◽  
Jiafang Xie ◽  
Hua Liu ◽  
...  
Keyword(s):  
Immune Response ◽  
Antigen Processing ◽  
Edwardsiella Tarda ◽  
Economic Losses ◽  
Pelteobagrus Fulvidraco ◽  
Yellow Catfish ◽  
Response Process ◽  
Apoptosis Pathway ◽  
Presentation Pathway ◽  
Immune Pathways

Edwardsiella tarda, the causative agent of ascites disease, is a major fish pathogen and has caused significant economic losses in aquaculture. To decipher the immune response process challenged by E. tarda in yellow catfish (Pelteobagrus fulvidraco), the transcriptomic profiles of the spleens infected with bacteria at 6 h, 24 h, and 72 h were obtained using the Illumina sequencing platform. After de novo assembly, a total of 158,124 unigenes were detected. To further investigate the immune-related DEGs, gene ontology (GO) enrichment and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis were performed. Immune pathways about antigen processing and presentation pathway, complement and coagulation cascades pathway, and apoptosis pathway were combined to discussed in this study. Additionally, 10 immune-related DEGs in these three immune pathways were randomly selected for expression verification by quantitative Real-time PCR (qRT-PCR). The data generated in this study provides a valuable resource for further immune response research and offers efficient strategies against E. tarda infection in yellow catfish.

scholarly journals Transcriptome Analysis of Paralichthys olivaceus Erythrocytes Reveals Profound Immune Responses Induced by Edwardsiella tarda Infection

2020 ◽  
Vol 21 (9) ◽  
pp. 3094
Author(s):  
Bin Sun ◽  
Xuepeng Li ◽  
Xianhui Ning ◽  
Li Sun
Keyword(s):  
Immune Response ◽  
Transcriptome Analysis ◽  
Antigen Processing ◽  
Paralichthys Olivaceus ◽  
Bacterial Pathogen ◽  
Infected Fish ◽  
Edwardsiella Tarda ◽  
Kegg Pathways

Unlike mammalian red blood cells (RBCs), fish RBCs are nucleated and thus capable of gene expression. Japanese flounder (Paralichthys olivaceus) is a species of marine fish with important economic values. Flounder are susceptible to Edwardsiella tarda, a severe bacterial pathogen that is able to infect and survive in flounder phagocytes. However, the infectivity of and the immune response induced by E. tarda in flounder RBCs are unclear. In the present research, we found that E. tarda was able to invade and replicate inside flounder RBCs in both in vitro and in vivo infections. To investigate the immune response induced by E. tarda in RBCs, transcriptome analysis of the spleen RBCs of flounder challenged with E. tarda was performed. Six sequencing libraries were constructed, and an average of 43 million clean reads per library were obtained, with 85% of the reads being successfully mapped to the genome of flounder. A total of 1720 differentially expressed genes (DEGs) were identified in E. tarda-infected fish. The DEGs were significantly enriched in diverse Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, especially those associated with immunity, disease, and infection. Ninety-one key DEGs involved in 12 immune-related pathways were found to form extensive interaction networks. Twenty-one genes that constituted the hub of the networks were further identified, which were highly regulated by E. tarda and involved in a number of immune processes, notably pathogen recognition and signal transduction, antigen processing and presentation, inflammation, and splicing. These results provide new insights into the immune role of flounder RBCs during bacterial infection.

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