Effect of Aloe vera Hydrogel Application on Increasing the Number of Fibroblasts in Socket Wounds Post-Tooth Extraction: An In Vivo Study

Background. Aloe vera has been used as an anti-inflammatory, antimicrobial, and immune-boosting agent since ancient time. Hydrogel is a polymeric material that can retain a significant amount of water component and can last as long as possible in the gingival sulcus because the hydrogel is hydrophilic. This study is the first study that seeks to explore the potential of Aloe vera in healing socket wounds after tooth extraction. Methods. Twenty-five white rats of the Wistar strain were acclimatized for seven days before being included in the study. After acclimatization, the experimental animals were grouped into five groups. Evaluation of fibroblast response process with HE staining. Results. There were differences in the mean number of fibroblasts in all groups. From the results of this study, it can be stated that there is an effect of the application of hydrogel Aloe vera 1%, 2.5%, 5%, 10%, and xylazine on the increase in the number of fibroblasts in socket wounds after tooth extraction in Rattus novergicus. Conclusion. There is an effect of Aloe vera hydrogel application on increasing the number of fibroblasts in socket wounds after tooth extraction in Rattus novergicus.

MATHEMATICAL MODEL OF UNDERWATER DEMINING BY DIPPER DIVERS OF THE SESU

It is shown that the problem of improving the effectiveness of prevention of emergencies related to the underwater location of explosive objects is relevant. An important and unresolved part of the problem is the lack of a mathematical model of the emergency response process associated with the underwater location of an explosive device in general. Based on this, the object of the study was the elimination of an emergency situation related to the underwater location of explosive objects, and the subject of the study – the process of operational activities of personnel of the underwater demining department of a group of special diving rescue team. The aim of the work is to develop a mathematical model of the emergency response process related to the underwater location of an explosive object as a process of functioning of the system "emergency – special means of underwater demining – diver-sapper", which should be the basis for substantiation of operational and technical recommendations. increasing the efficiency of underwater demining by diver sappers without reducing their level of safety. It is shown that the mathematical model of underwater demining by a diver-sapper is a system of three analytical dependences. The first is a functional that describes the process of underwater demining in the form of a three-factor polynomial model. The second allows us to present this functionality as a set of one-factor models. The third provides the definition of weights in solving a multifactor problem. It is noted that such a model allows to proceed to the substantiation of operational and technical recommendations to the management of the group of special diving works. The advantage of the new scientific result is the ability to obtain both quantitative estimates of the impact of the direct components of the system "diver-sapper – special means of underwater demining – underwater location of an explosive object" and their relationship. The disadvantage is the large number of experimental results that must be obtained to implement the selected plan.

Transcriptome Analysis of Immune Response Process in Yellow Catfish (Pelteobagrus fulvidraco) During Edwardsiella tarda Exposure

Edwardsiella tarda, the causative agent of ascites disease, is a major fish pathogen and has caused significant economic losses in aquaculture. To decipher the immune response process challenged by E. tarda in yellow catfish (Pelteobagrus fulvidraco), the transcriptomic profiles of the spleens infected with bacteria at 6 h, 24 h, and 72 h were obtained using the Illumina sequencing platform. After de novo assembly, a total of 158,124 unigenes were detected. To further investigate the immune-related DEGs, gene ontology (GO) enrichment and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis were performed. Immune pathways about antigen processing and presentation pathway, complement and coagulation cascades pathway, and apoptosis pathway were combined to discussed in this study. Additionally, 10 immune-related DEGs in these three immune pathways were randomly selected for expression verification by quantitative Real-time PCR (qRT-PCR). The data generated in this study provides a valuable resource for further immune response research and offers efficient strategies against E. tarda infection in yellow catfish.