scholarly journals Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Malaysian Rice (cv. MR219) for Lethal Dose Determination

2012 ◽  
Vol 03 (12) ◽  
pp. 1661-1665 ◽  
Author(s):  
Ali Benjavad Talebi ◽  
Amin Benjavad Talebi ◽  
Behzad Shahrokhifar
Keyword(s):  
Lethal Dose ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Dose Determination ◽  
Methane Sulphonate ◽  
Induced Mutagenesis

scholarly journals Induced mutagenesis in Chickpea (Cicer arietinum (L.) with special reference to the frequency and spectrum of chlorophyll mutations

2016 ◽  
Vol 1 (1) ◽  
pp. 49 ◽  
Author(s):  
S. Umavathi ◽  
L. Mullainathan
Keyword(s):  
Cicer Arietinum ◽  
Gamma Rays ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Cicer Arietinum L ◽  
Chlorophyll Mutations ◽  
Methane Sulphonate ◽  
Chlorophyll Mutants ◽  
Induced Mutagenesis ◽  
Different Doses

A relative study of frequency and spectrum of chlorophyll mutations induced by mutagens in M2 generation was made with chickpea (Cicer arietinum (L). Variety ‘CO-4’. The treatments include different doses/concentrations of Gamma rays (20, 30, 40, 50 and 60kR) and Ethyl Methane Sulphonate (10, 20, 30, 40 and 50 mM). From the study, the overall frequencies and spectrum of five types of induced chlorophyll mutants Viridis (0.55), Xantha (0.46), Chlorina (0.45), Albina (0.43) and Tigrina (0.35) were observed. The frequency of chlorophyll mutation was increased with increasing concentrations up to a level, beyond it declined in both the mutagens. And the chlorophyll frequency was found in the order of viridis > xantha >chlorina> Albina >tigrina. The chemical mutagen, EMS was found to be more effective in inducing chlorophyll mutations than gamma rays in Chick pea.

scholarly journals Effects of gamma irradiation and ethyl methane sulphonate on morphometric traits and prevalence of common viral diseases and whiteflies in cassava.

1970 ◽  
Vol 29 (3) ◽  
pp. 355-371
Author(s):  
J.K. Baguma ◽  
E. Ogwok ◽  
W. Elegba ◽  
A. Sarkodie ◽  
S. Otu ◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Cassava Mosaic Disease ◽  
Viral Diseases ◽  
Ethyl Methane Sulphonate ◽  
Morphometric Traits ◽  
Shoot Height ◽  
Disease Symptoms ◽  
Ethyl Methane ◽  
Methane Sulphonate ◽  
Induced Mutagenesis

Cassava (Manihot esculenta Crantz) is an important staple and food security crop for millions of people in Africa. However, its nutritional value is limited; yet its productivity is constrained by several pests and diseases. Induced mutagenesis is one approach with the potential to overcome such biotic stresses. The objective of this study was to assess the variability in morphometric traits and prevalence of common viral diseases and whiteflies in cassava to different doses and concentrations of gamma irradiation and ethyl methane sulphonate (ems) treatments. The effects were assessed on seed germination and growth of stakes, as well as foliar viral disease symptoms and whitefly counts. Radio-sensitivity tests revealed LD50 for sprouting as 37.6Gy for g-irradiation and 0.08% for EMS treatment. There was notable decrease in sprout, epicotyl length, shoot height, petiole length and number of leaf lobes, with increasing g-ray doses and EMS concentrations. Contrastingly, total chlorophyll content increased with increasing doses of g-rays and EMS concentrations. Basing on foliar whitefly counts and disease symptoms, there was a general increase in susceptibility to whitefly infestation and cassava mosaic disease (CMD) incidence. Significantly varying levels of resistance or tolerance to whiteflies and CMD were observed among plantlets derived from irradiated and EMS treated stems, compared to the controls. These findings lay a foundation for more future research on breeding for various traits, including disease resistance in cassava using induced mutagenesis approach.

Evaluation of Yield Attributing Variants Developed Through Ethyl Methane Sulphonate in an Important Proteinaceous Crop-Vicia faba

2017 ◽  
Vol 9 (2) ◽  
pp. 20-27 ◽  
Author(s):  
Durre Shahwar ◽  
Mohammad Yunus Khali Ansari ◽  
Sana Chaudhary ◽  
Rumana Aslam
Keyword(s):  
Vicia Faba ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Methane Sulphonate

scholarly journals Induced Mutagenesis Enhances Lodging Resistance and Photosynthetic Efficiency of Kodomillet (Paspalum Scrobiculatum)

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 227 ◽  
Author(s):  
James Poornima Jency ◽  
Ravikesavan Rajasekaran ◽  
Roshan Kumar Singh ◽  
Raveendran Muthurajan ◽  
Jeyakumar Prabhakaran ◽  
...  
Keyword(s):  
Expression Profiling ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Ethyl Methane Sulphonate ◽  
Lodging Resistance ◽  
Pyruvate Phosphate Dikinase ◽  
Photosynthetic System ◽  
Methane Sulphonate ◽  
Induced Mutagenesis

The present research was focused in the development of photosynthetically efficient (PhE) and non-lodging mutants by utilizing ethyl methane sulphonate (EMS) and gamma radiation in the kodomillet variety CO 3, prone to lodging. Striking variations in a number of anatomical characteristics of leaf anatomy for PhE and culm thickness for lodging resistance was recorded in M2 (second mutant) generation. The identified mutants were subjected to transcriptomic studies to understand their molecular basis. Expression profiling was undertaken for pyruvate phosphate dikinase (PPDK), Nicotinamide Adenine Dinucleotide Phosphate Hydrogen—(NADPH) and NADP-dependent malate dehydrogenase (NADP-MDH) in the mutants CO 3-100-7-12 (photosynthetically efficient) and in CO 3-200-13-4 (less efficient). For lodging trait, two mutants CO 3-100-18-22 (lodged) and CO 3-300-7-4 (non-lodged) were selected for expression profiling using genes GA2ox6 and Rht-B. The studies confirmed the expression of PPDK increased 30-fold, NADP-ME2 ~1-fold and NADP-MDH10 was also highly expressed in the mutant CO 3-100-7-12. These expression profiles suggest that kodomillet uses an NADP-malic enzyme subtype C4 photosynthetic system. The expression of Rht-B was significantly up regulated in CO 3-300-7-4. The study highlights the differential expression patterns of the same gene in different lines at different time points of stress as well as non-stress conditions. This infers that the mutation has some effect on their expression; otherwise the expression levels will be unaltered. Enhancement in grain yield could be best achieved by developing a phenotype with high PhE and culm with thick sclerenchyma cells.

Effect of seed treatment by ethyl methane sulphonate on growth, flowering and yield of papaya cv. Pusa Dwarf

2016 ◽  
Vol 7 (1) ◽  
pp. 64
Author(s):  
Mahesh Kumar ◽  
Mukesh Kumar ◽  
Satya Prakash ◽  
DK Gautam ◽  
Sanjeev Rao
Keyword(s):  
Seed Treatment ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Methane Sulphonate
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