scholarly journals Does Photofrin II Combined with a Radio-Adaptive Dose Lead to a Synergistic or Additive Effect after Ionising Irradiation <i>In Vitro</i>?

2011 ◽  
Vol 02 (04) ◽  
pp. 595-600
Author(s):  
Moshe Schaffer ◽  
Alina Balandin ◽  
Birgit Ertl-Wagner ◽  
Pamela Schaffer ◽  
Luigi Bonavina ◽  
...  
Keyword(s):  
Additive Effect ◽  
Photofrin Ii

Radiofrequency impairs viability of bladder cancer cells and has an additive effect when combined with chemotherapeutics in vitro

2019 ◽  
Vol 18 (8) ◽  
pp. e3152-e3153
Author(s):  
I.S.G. Brummelhuis ◽  
G.M. Lev ◽  
J.W. Van Hattum ◽  
J.A. Witjes ◽  
E. Oosterwijk
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Additive Effect ◽  
Bladder Cancer Cells

Study of the in vivo and in vitro photosensitizing capabilities of uroporphyrin I compared to photofrin II

1986 ◽  
Vol 6 (2) ◽  
pp. 131-136 ◽  
Author(s):  
J. Stuart Nelson ◽  
Chung-Ho C. Sun ◽  
Michael W. Berns
Keyword(s):  
Photofrin Ii

scholarly journals Putative Inv Is Essential for Basolateral Invasion of Caco-2 Cells and Acts Synergistically with OmpA To AffectIn VitroandIn VivoVirulence of Cronobacter sakazakii ATCC 29544

2014 ◽  
Vol 82 (5) ◽  
pp. 1755-1765 ◽  
Author(s):  
Dilini Chandrapala ◽  
Kyumson Kim ◽  
Younho Choi ◽  
Amal Senevirathne ◽  
Dong-Hyun Kang ◽  
...  
Keyword(s):  
Outer Membrane Proteins ◽  
Opportunistic Pathogen ◽  
Additive Effect ◽  
Neonatal Meningitis ◽  
Cronobacter Sakazakii ◽  
Content Type ◽  
Enteric Infections ◽  
Culture Models

ABSTRACTCronobacter sakazakiiis an opportunistic pathogen that causes neonatal meningitis and necrotizing enterocolitis. Its interaction with intestinal epithelium is important in the pathogenesis of enteric infections. In this study, we investigated the involvement of theinvgene in the virulence ofC. sakazakiiATCC 29544in vitroandin vivo. Sequence analysis ofC. sakazakiiATCC 29544invrevealed that it is different from otherC. sakazakiiisolates. In various cell culture models, an Δinvdeletion mutant showed significantly lowered invasion efficiency, which was restored upon genetic complementation. Studying invasion potentials using tight-junction-disrupted Caco-2 cells suggested that theinvgene product mediates basolateral invasion ofC. sakazakiiATCC 29544. In addition, comparison of invasion potentials of double mutant (ΔompA Δinv) and single mutants (ΔompAand Δinv) provided evidence for an additive effect of the two putative outer membrane proteins. Finally, the importance ofinvand the additive effect of putative Inv and OmpA were also proven in anin vivorat pup model. This report is the first to demonstrate two proteins working synergisticallyin vitro, as well asin vivoinC. sakazakiipathogenesis.

The influence of oxygen on the radiosensitizing activity of Photofrin II and hypericin

2007 ◽  
Vol 11 (10) ◽  
pp. 736-741 ◽  
Author(s):  
Pamela Schaffer ◽  
Ulrike Kulka ◽  
Birgit Ertl-Wagner ◽  
Roswita Hell ◽  
Alina Balandin ◽  
...  
Keyword(s):  
Carcinoma Cell ◽  
Cell Damage ◽  
Human Bladder ◽  
Photofrin Ii ◽  
Carcinoma Cell Lines ◽  
Radiation Induced ◽  
Low Levels ◽  
Sufficient Oxygen

Several clinical studies, as well as investigations performed on tissue cultures and murine tumor models, have demonstrated the in vitro and in vivo efficacy of Photofrin II and hypericin as radiosensitizing agents. The mechanisms involved in the radiosensitizing action of Photofrin II and hypericin are partially understood; the recognition of the major role performed by oxygen regarding the modulation of cellular radiosensitivity has prompted the present investigations on the relevance of oxygenation for the success of Photofrin II or hypericin-based radiation therapy of tumors. RT4 human bladder carcinoma cell lines were seeded and incubated with various concentrations of Photofrin II or hypericin under ambient and 5% oxygen levels. The cells were irradiated with ionizing radiation between 1 and 6 Gy. The same experiments were repeated with Photofrin II and hypericin alone, without radiation. The cell survival was evaluated. The results demonstrated an increase of radiation-induced cell damage in the presence of Photofrin II and hypericin, respectively, when sufficient oxygen was available. Low levels of oxygen reduced the activity of Photofrin II as well as of hypericin as a radiosensitizer, with minimal tumor damage ( p < 0.05 in a Student t-test). The mechanism of action of Photofrin II and hypericin as radiosensitizers requires the presence of sufficiently high oxygen concentrations.

Photosensitization by the Near-IR-absorbing Photosensitizer Lutetium Texaphyrin: Spectroscopic, In Vitro and In Vivo Studies

1998 ◽  
Vol 02 (05) ◽  
pp. 383-390 ◽  
Author(s):  
GENADY KOSTENICH ◽  
TANYA BABUSHKINA ◽  
ADINA LAVI ◽  
YAKOV LANGZAM ◽  
ZVI MALIK ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Structural Damage ◽  
Biological Properties ◽  
In Vivo Studies ◽  
Outer Cell ◽  
Lipid Bilayer Membranes ◽  
Photofrin Ii ◽  
Ionic Imbalance

The spectroscopic and biological properties of the new photosensitizer lutetium texaphyrin (Lu-Tex) were assessed in vitro and in vivo on a C26 colon carcinoma model, in comparison with hematoporphyrin (Hp), photofrin II (PII) and chlorin e 6( Chl ). Strong binding of Lu-Tex to lipid bilayer membranes was observed. The results of confocal fluorescence microscopy on C26 cells showed that Lu-Tex was localized in small vesicles in the cytoplasm, possibly in the lysosomes, while Chl and Hp were distributed in larger cytoplasmic vesicles attributed to mitochondria. Scanning electron microscopy and X-ray microanalysis revealed that photodynamic therapy with Lu-Tex induced only slight damage to the cell membrane, leading to a delayed cell response. Chl and Hp caused significant structural damage to the outer cell membrane, resulting in ionic imbalance and fast cell death. The in vitro quantitative assessment of the relative efficiency per absorbed photon of the sensitizers revealed that Lu-Tex was less effective than Chl and Hp . However, the results of our in vivo study showed that at the same light and drug doses the anti-tumor efficiency of the agents was in the following order: Lu-Tex > Chl > PII . The strong in vivo anti-tumor effect of Lu-Tex can be explained by its higher integrated absorption in the long-wavelength range.

scholarly journals Additive Effect of Dornase Alfa and Nacystelyn on Transportability and Viscoelasticity of Cystic Fibrosis Sputum

2002 ◽  
Vol 9 (6) ◽  
pp. 401-406 ◽  
Author(s):  
Feng Sun ◽  
Shusheng Tai ◽  
Thomas Lim ◽  
Ulrich Baumann ◽  
Malcolm King
Keyword(s):  
Cystic Fibrosis ◽  
Normal Saline ◽  
Additive Effect ◽  
Extracellular Dna ◽  
Additional Increase ◽  
Dornase Alfa ◽  
Additive Combination ◽  
Drug Solution

OBJECTIVE: To investigate the effect of dornase alfa (DA), Nacystelyn (NAL) and their combination on mucociliary transportability and mucus viscoelasticity of cystic fibrosis (CF) sputum, and to assess whether the combination possesses an additive effect.DESIGN: Determination of transportability in frog palate and viscoelasticity in vitro.SETTING: Research laboratory at a medical centre. Patients: Sputa from 15 patients with CF, chronically infected with Pseudomonas aeruginosa, were studied.INTERVENTIONS: Sputum samples were incubated without any drug solution as a control, and with normal saline, DA, NAL and a mixture of DA and NAL in concentrations approximating those achieved in clinical practice.RESULTS: Normal saline (10% volume) by itself had a small effect on CF sputum transportability with a mean increase of 9%, and on viscoelasticity with a mean of decrease of 0.22 log units, respectively, compared with control (incubation without saline). DA (200 nM) further increased the transportability by a mean of 35% versus saline and decreased viscoelasticity by a mean of 0.30 log units. NAL (100 µM) increased the transportability by a mean of 32% and decreased viscoelasticity by a mean of 0.22 log units from the levels achieved with saline. The mixture of DA plus NAL at one-half of the above concentration of each agent produced an additional increase in the transportability, by a mean of 18%, and a further decrease in viscoelasticity, by a mean of 0.25 log units, compared with DA or NAL as a single treatment.CONCLUSIONS: The combination of DA and NAL exhibits an additive effect for both the viscoelasticity and transportability of CF sputum samples. The two agents appear to act well together in breaking down the bonding due to extracellular DNA and mucins. Clinical studies should be undertaken to see whether the additive combination at lower concentration produces the anticipated benefits of improved airway clearance and fewer side effects.

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