Preventive effect of Catharanthus roseus (Linn.) against high-fructose diet-induced insulin resistance and oxida-tive stress in male Wistar rats

Purpose: To examine the potential of extracts from selected herbs used in African traditional medicine in diabetes patients, and to determine their effect on traits of metabolic syndrome in rats fed a high-fat and high-fructose diet.Methods: Ethanol and aqueous extracts were prepared from Mangifera indica (MI), Lonchocarpus eriocalyx (LE), Urtica massaica (UM), Schkuhria pinnata (SP) and Launaea cornuta (LC). Ethanol extracts (1:100 dilution) were examined for inhibition of pancreatic lipase and α-glucosidase activity invitro. Furthermore, aqueous extracts were administered for 74 days to male Wistar rats fed a high-fat and high-fructose diet to assess their effect on traits of metabolic syndrome.Results: Ethanol extracts showed at least 30 % inhibition of pancreatic lipase in vitro but no effect on α- glucosidase activity. Administration of the aqueous extracts caused significant reduction in liver triglycerides (except for LE). Muscle triglycerides and fat were also reduced, with the most pronounced effect elicited by LE. Urinary glucose excretion and plasma triglycerides, but not hyperinsulinemia and insulin resistance, were reduced by UM compared to control.Conclusion: This exploratory study indicates that UM may be considered a candidate for the prevention and management of type 2 diabetes. Keywords: Kenyan traditional medicine, High-fat diet, High fructose, Insulin resistance, Triglycerides, Diabetes, Liver steatosis

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Endothelin-1 blockade prevents COX2 induction and TXA2 production in the fructose hypertensive ratThis paper is one of a selection of papers published in this Special Issue, entitled The Cellular and Molecular Basis of Cardiovascular Dysfunction, Dhalla 70th Birthday Tribute.

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y06-088 ◽

2007 ◽

Vol 85 (3-4) ◽

pp. 422-429 ◽

Cited By ~ 11

Author(s):

Jihong Jiang ◽

Linda Tran ◽

Harish Vasudevan ◽

Zhengyuan Xia ◽

Violet G. Yuen ◽

...

Keyword(s):

Insulin Resistance ◽

Control Level ◽

Total Duration ◽

Endothelin 1 ◽

High Fructose Diet ◽

Thromboxane Synthase Inhibitor ◽

High Fructose ◽

Male Wistar Rats ◽

Synthase Inhibitor

Feeding rats with a high fructose diet results in insulin resistance and hypertension. Fructose-hypertensive rats (FHR) have increased vascular levels of endothelin-1 (ET-1) and thromboxane (TXA2). We have previously shown that chronic treatment with either the dual endothelin receptor blocker, bosentan, or the thromboxane synthase inhibitor, dazmegrel, prevented fructose-induced increases in blood pressure, suggesting that both ET-1 and TXA2 play important roles in the development of hyperinsulinemia/insulin resistance-associated hypertension. In this study, we investigated the potential interrelationship between ET-1 and TXA2 in the development of fructose-induced hypertension in vivo. Male Wistar rats were fed on a high fructose diet for 9 weeks. Either bosentan or dazmegrel treatment (daily by oral gavage) was initiated 3 weeks after the start of fructose feeding for a total duration of 6 weeks. At the end of drug treatment, blood and aorta were collected from each animal. Plasma thromboxane B2 (TXB2), a stable TXA2 metabolite, increased significantly in FHR and was reduced to control level by both chronic bosentan and dazmegrel treatment. Protein expression of cyclooxygenase 2 (COX2) was elevated significantly in FHR aortas and treatment with bosentan and dazmegrel corrected these changes. These results indicate that the actions of ET-1 in the aorta of FHR may be mediated through COX2-derived TXA2. Bosentan may prevent the development of hypertension in fructose-fed rats through inhibition of COX2 induction and subsequently the reduction in plasma TXA2.

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Insulin resistance induced by post-weaning high fructose diet in Wistar rats: reversal by metformin

Reproduction Nutrition Development ◽

10.1051/rnd:19940635 ◽

1994 ◽

Vol 34 (6) ◽

pp. 629-630

Author(s):

S. Halimi ◽

E. Rossini ◽

PY Benhamou ◽

P. Faure ◽

P. André

Keyword(s):

Insulin Resistance ◽

Wistar Rats ◽

High Fructose Diet ◽

High Fructose

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High-fructose diet during puberty alters the sperm parameters, testosterone concentration, and histopathology of testes and epididymis in adult Wistar rats

Journal of Developmental Origins of Health and Disease ◽

10.1017/s2040174420001385 ◽

2021 ◽

pp. 1-8

Author(s):

Daniele Sapede Alvarenga Medaglia ◽

Henrique Rodrigues Vieira ◽

Sandra da Silva Silveira ◽

Gláucia Eloisa Munhoz de L. Siervo ◽

Monique Suellen da Silva Marcon ◽

...

Keyword(s):

Reproductive System ◽

Wistar Rats ◽

Metabolic Diseases ◽

Serum Testosterone ◽

Seminiferous Tubules ◽

Adult Rats ◽

High Fructose Diet ◽

Fructose Intake ◽

High Fructose ◽

Male Wistar Rats

Abstract The consumption of fructose has increased in children and adolescents and is partially responsible for the high incidence of metabolic diseases. The lifestyle during postnatal development can result in altered metabolic programming, thereby impairing the reproductive system and fertility during adulthood. Therefore, the aim of this study was to evaluate the effect of a high-fructose diet in the male reproductive system of pubertal and adult rats. Male Wistar rats (30 d old) were assigned to four different groups: Fr30, which received fructose (20%) in water for 30 d and were euthanized at postnatal day (PND) 60; Re-Fr30, which received fructose (20%) for 30 d and were euthanized at PND 120; and two control groups C30 and Re-C30, which received water ad libitum and were euthanized at PND 60 and 120, respectively. Fructose induced an increase in abnormal seminiferous tubules with epithelial vacuoles, degeneration, and immature cells in the lumen. Moreover, Fr30 rats showed altered spermatogenesis and daily sperm production (DSP), as well as increased serum testosterone concentrations. After discontinuing high-fructose consumption, DSP and sperm number decreased significantly. We observed tissue remodeling in the epididymis, with a reduction in stromal and epithelial compartments that might have influenced sperm motility. Therefore, we concluded that fructose intake in peripubertal rats led to changes in the reproductive system observed both during puberty and adulthood.

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Germinated millet flour (Pennisetum Glaucum (L.) R. BR.) improves adipogenesis and glucose metabolism and maintains thyroid function in vivo

Food & Function ◽

10.1039/d0fo03388j ◽

2021 ◽

Author(s):

Jaqueline Maciel Vieira Theodoro ◽

Oscar David Medina Martinez ◽

Mariana Grancieri ◽

Renata Celi Lopes Toledo ◽

Mirella Lima Binoti ◽

...

Keyword(s):

Insulin Resistance ◽

Glucose Metabolism ◽

Glucose Tolerance ◽

Thyroid Function ◽

Wistar Rats ◽

Pennisetum Glaucum ◽

High Fat ◽

High Fructose Diet ◽

High Fructose

This study investigated the effects of germinated millet flour on adipogenesis, insulin resistance, glucose tolerance and thyroid function in Wistar rats fed with a high-fat high-fructose diet (HFHF). The experiment...

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