Physiological evaluation of the responses of Larix olgensis families to drought stress and proteomic analysis of the superior family

Drought is a major adversity that limits crop yields. Further exploration of wheat drought tolerance-related genes is critical for the genetic improvement of drought tolerance in this crop. Here, comparative proteomic analysis of two wheat varieties, XN979 and LA379, with contrasting drought tolerance was conducted to screen for drought tolerance-related proteins/genes. Virus-induced gene silencing (VIGS) technology was used to verify the functions of candidate proteins. A total of 335 differentially abundant proteins (DAPs) were exclusively identified in the drought-tolerant variety XN979. Most DAPs were mainly involved in photosynthesis, carbon fixation, glyoxylate and dicarboxylate metabolism, and several other pathways. Two DAPs (W5DYH0 and W5ERN8), dubbed TaDrSR1 and TaDrSR2, respectively, were selected for further functional analysis using VIGS. The relative electrolyte leakage rate and malonaldehyde content increased significantly, while the relative water content and proline content significantly decreased in the TaDrSR1- and TaDrSR2-knock-down plants compared to that in non-knocked-down plants under drought stress conditions. TaDrSR1- and TaDrSR2-knock-down plants exhibited more severe drooping and wilting phenotypes than non-knocked-down plants under drought stress conditions, suggesting that the former were more sensitive to drought stress. These results indicate that TaDrSR1 and TaDrSR2 potentially play vital roles in conferring drought tolerance in common wheat.

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Comparative physiological and proteomic analysis indicates lower shock response to drought stress conditions in a self-pollinating perennial ryegrass

PLoS ONE ◽

10.1371/journal.pone.0234317 ◽

2020 ◽

Vol 15 (6) ◽

pp. e0234317 ◽

Cited By ~ 2

Author(s):

Fatemeh Raeisi Vanani ◽

Leila Shabani ◽

Mohammad R. Sabzalian ◽

Fariba Dehghanian ◽

Lisa Winner

Keyword(s):

Drought Stress ◽

Perennial Ryegrass ◽

Proteomic Analysis ◽

Stress Conditions ◽

Shock Response

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Spatiotemporal Expression and Bioinformatic Analyses of the HD-Zip Transcription Factor Family in Larix olgensis

Plant Molecular Biology Reporter ◽

10.1007/s11105-020-01244-9 ◽

2020 ◽

Author(s):

Peiqi An ◽

Qing Cao ◽

Chen Wang ◽

Junhun Wang ◽

Hanguo Zhang ◽

...

Keyword(s):

Transcription Factor ◽

Drought Stress ◽

Stress Responses ◽

Secondary Growth ◽

Larix Olgensis ◽

Transcription Factor Family ◽

Real Time Quantitative Pcr ◽

Spatiotemporal Expression ◽

Zip Family ◽

Stems And Leaves

Abstract Larix olgensis is one of the main coniferous tree species in northeastern China and has excellent timber properties and strong tolerance to stress. Thirteen HD-Zip family genes with a complete CDS region were identified on the basis of cambium transcriptome data from Larix olgensis. All 13 genes were analyzed via bioinformatics by their conserved domain protein sequence and amino acid composition, including their physicochemical properties and protein structure. The spatiotemporal expression and abiotic stress responses of these genes were analyzed by real-time quantitative PCR. The results showed that the 13 HD-Zip genes of Larix olgensis were expressed in the roots, stems, and leaves at different stages. The expression of three of these genes (LoHDZ2, LoHDZ11, LoHDZ13) was highest in nonlignified roots, indicating that they might be related to the secondary growth of Larix olgensis; in addition, three genes (LoHDZ5, LoHDZ9, LoHDZ10) were highly expressed in partially and completely lignified stems and leaves. These 13 genes were expressed specifically under drought stress. The expression of two of them (LoHDZ1, LoHDZ5) was obviously upregulated, and the expression of 6 genes (LoHDZ2, LoHDZ3, LoHDZ4, LoHDZ8, LoHDZ10, LoHDZ13) was significantly downregulated. The expression trends indicate that these genes could be involved in drought stress. The expression of all 13 genes was downregulated when the plants were treated with 0.2 M NaCl for 96 h, indicating that these genes are inhibited by salt stress. Overall, the results have significant implications for the study of the gene function of members of the LoHD-Zip transcription factor family.

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