Preliminary study of the effects of β-elemene on MCF-7/ADM breast cancer stem cells

Breast cancer stem cells (CSCs) are highly tumorigenic and possess the capacity to self-renew. Recent studies indicated that pluripotent geneNANOGinvolves in regulating self-renewal of breast CSCs, and expression of NANOG is correlated with aggressiveness of poorly differentiated breast cancer. We initially confirmed that breast cancer MCF-7 cells expressed NANOG, and overexpression of NANOG enhanced the tumorigenicity of MCF-7 cells and promoted the self-renewal expansion of CD24−/lowCD44+CSC subpopulation. In contrast, knockdown of NANOG significantly affected the growth of breast CSCs. Utilizing flow cytometry, we identified five cyclohexylmethyl flavonoids that can inhibit propagation of NANOG-positive cells in both breast cancer MCF-7 and MDA-MB231 cells. Among these flavonoids, ugonins J and K were found to be able to induce apoptosis in non-CSC populations and to reduce self-renewal growth of CD24−/lowCD44+CSC population. Treatment with ugonin J significantly reduced the tumorigenicity of MCF-7 cells and efficiently suppressed formation of mammospheres. This suppression was possibly due to p53 activation and NANOG reduction as either addition of p53 inhibitor or overexpression of NANOG can counteract the suppressive effect of ugonin J. We therefore conclude that cyclohexylmethyl flavonoids can possibly be utilized to suppress the propagation of breast CSCs via reduction of NANOG.

Download Full-text

Monobenzyltin Complex C1 Induces Apoptosis in MCF-7 Breast Cancer Cells through the Intrinsic Signaling Pathway and through the Targeting of MCF-7-Derived Breast Cancer Stem Cells via the Wnt/β-Catenin Signaling Pathway

PLoS ONE ◽

10.1371/journal.pone.0160836 ◽

2016 ◽

Vol 11 (8) ◽

pp. e0160836 ◽

Cited By ~ 10

Author(s):

Somayeh Fani ◽

Firouzeh Dehghan ◽

Hamed Karimian ◽

Kong Mun Lo ◽

Siyamak Ebrahimi Nigjeh ◽

...

Keyword(s):

Breast Cancer ◽

Stem Cells ◽

Cancer Stem Cells ◽

Cancer Cells ◽

Signaling Pathway ◽

Breast Cancer Cells ◽

Breast Cancer Stem Cells ◽

Mcf 7

Download Full-text

Curcumin and Glu-GNPs Induce Radiosensitivity against Breast Cancer Stem-Like Cells

BioMed Research International ◽

10.1155/2020/3189217 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Ke Yang ◽

Zhiwei Liao ◽

Yujian Wu ◽

Mengjie Li ◽

Tingting Guo ◽

...

Keyword(s):

Breast Cancer ◽

Stem Cells ◽

Cancer Stem Cells ◽

High Efficiency ◽

Tumor Hypoxia ◽

Breast Cancer Stem Cells ◽

Breast Cancer Patients ◽

Breast Cancer Radiotherapy ◽

Radiotherapy Resistance ◽

Mcf 7

Breast cancer stem cells are an important cause of radiotherapy resistance in the clinical treatment of breast cancer patients. How to target breast cancer stem cells is the key to improving the efficacy of breast cancer radiotherapy. We proposed for the first time that curcumin combined with glucose nanogold particles (Glu-GNPs) targeted breast cancer stem cells to reduce radiotherapy resistance, which can significantly enhance the apoptosis level of MCF-7 and MDA-MB-231 breast cancer stem-like cells (BCSCs) after radiotherapy and antiproliferation and colony-forming. Under simulated hypoxic conditions, curcumin combined with Glu-GNPs can significantly improve the ROS level of MCF-7 and MDA-MB-231 mammospheres; reduce the expression of HIF-1α and HSP90, thereby inhibiting the tumor cells’ own stress ability; promote the apoptosis of tumor stem cells; and enhance the sensitivity of radiotherapy. The current results indicate that the combination of curcumin and Glu-GNPs has great potential to relieve tumor hypoxia and increase radiosensitivity on BCSCs, providing scientific research data for developing a novel radiosensitizer with high efficiency and low toxicity.

Download Full-text

Peer Review #2 of "Breast fibroblasts in both cancer and normal tissues induce phenotypic transformation of breast cancer stem cells: a preliminary study (v0.2)"

10.7287/peerj.4805v0.2/reviews/2 ◽

2018 ◽

Keyword(s):

Breast Cancer ◽

Stem Cells ◽

Cancer Stem Cells ◽

Peer Review ◽

Breast Cancer Stem Cells ◽

Normal Tissues ◽

Phenotypic Transformation ◽

Preliminary Study ◽

Breast Fibroblasts

Download Full-text

Role of E1B protein-dificient oncolytic virus in breast cancer stem cells

Journal of Clinical Oncology ◽

10.1200/jco.2009.27.15_suppl.e22131 ◽

2009 ◽

Vol 27 (15_suppl) ◽

pp. e22131-e22131

Author(s):

F. Zhang ◽

Y. Ma ◽

Y. Xu ◽

M. Huang ◽

C. Song ◽

...

Keyword(s):

Breast Cancer ◽

Stem Cells ◽

Cancer Stem Cells ◽

Oncolytic Adenovirus ◽

Infected Group ◽

Control Group ◽

Breast Cancer Stem Cells ◽

Differentiated Cells ◽

Mcf 7

e22131 Background: Cancer stem cells have been indicated in the initiation of tumors and are even found to be responsible for relapses after apparently curative therapies have been undertaken. In breast cancer, they may reside in the CD44+CD24−/low population. Oncolytic adenoviruses enter cells through infection and can kill both proliferating and quiescent cells. We investigated the role of E1B protein- dificient oncolytic adenovirus in breast cancer stem cells. Methods: MCF-7 cells were infected by E1B protein-dificient oncolytic adenovirus as infected group (MOI=100) and cultured routinely as control group simultaneously. The proportion of CD44+CD24- cells was assessed by flow cytometry (FCM) in two groups respectively. Meanwhile, mammosphere culture was done in two groups' cells to observe the size and number of mammospheres, calculate the mammosphere- forming efficiency (MFE). The proportion of CD44+CD24- cells in two groups' mammospheres was assessed by FCM. Results: The percentages of CD24-,CD44+, CD44+CD24- in the infected gruop were 43.9%, 63.26%, 22.19%, respectively. While in the control group, the percentages were 6.74%, 88.30%, 2.30%. In the infected group, the time of mammosphere's formation was earlier, the volumes of mammospheres were bigger and the MFE was higher than the control group (1.26%:0.9%). In two mammospheres' groups, the proportion of CD44+CD24- cells in experiment group and control group was 38.08% and 23.35%, respectively. Conclusions: E1B protein-dificient oncolytic adenovirus can kill MCF-7 cells in short time, mainly breast cancer differentiated cells. It maybe promote the growth of the breast cancer stem cells. It maybe accelerate the speed of self-renewed and differentiation of the breast cancer stem cells. No significant financial relationships to disclose.

Download Full-text