scholarly journals Multilineage Differentiation of Dental Pulp Stem Cells from Green Fluorescent Protein Transgenic Mice

2010 ◽  
Vol 2 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Brian E Grottkau ◽  
P Prasad Purudappa ◽  
Yun‐feng Lin
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Dental Pulp ◽  
Fluorescent Protein ◽  
Dental Pulp Stem Cells ◽  
Multilineage Differentiation ◽  
Green Fluorescent

Characterisation of the deleted in azoospermia like (Dazl)–green fluorescent protein mouse model generated by a two-step embryonic stem cell-based strategy to identify pluripotent and germ cells

2016 ◽  
Vol 28 (11) ◽  
pp. 1741 ◽  
Author(s):  
Priscila Ramos-Ibeas ◽  
Eva Pericuesta ◽  
Raúl Fernández-González ◽  
Alfonso Gutiérrez-Adán ◽  
Miguel Ángel Ramírez
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Germ Cells ◽  
Fluorescent Protein ◽  
Preimplantation Embryo ◽  
Embryonic Stem ◽  
Deleted In Azoospermia ◽  
Green Fluorescent

The deleted in azoospermia like (Dazl) gene is preferentially expressed in germ cells; however, recent studies indicate that it may have pluripotency-related functions. We generated Dazl–green fluorescent protein (GFP) transgenic mice and assayed the ability of Dazl-driven GFP to mark preimplantation embryo development, fetal, neonatal and adult tissues, and in vitro differentiation from embryonic stem cells (ESCs) to embryoid bodies (EBs) and to primordial germ cell (PGC)-like cells. The Dazl-GFP mice were generated by a two-step ESC-based strategy, which enabled primary and secondary screening of stably transfected clones before embryo injection. During preimplantation embryo stages, GFP was detected from the zygote to blastocyst stage. At Embryonic Day (E) 12.5, GFP was expressed in gonadal ridges and in neonatal gonads of both sexes. In adult mice, GFP expression was found during spermatogenesis from spermatogonia to elongating spermatids and in the cytoplasm of oocytes. However, GFP mRNA was also detected in other tissues harbouring multipotent cells, such as the intestine and bone marrow. Fluorescence was maintained along in vitro Dazl-GFP ESC differentiation to EBs, and in PGC-like cells. In addition to its largely known function in germ cell development, Dazl could have an additional role in pluripotency, supporting these transgenic mice as a valuable tool for the prospective identification of stem cells from several tissues.

scholarly journals Embryonic Stem Cells and Transgenic Mice Ubiquitously Expressing a Tau-Tagged Green Fluorescent Protein

2000 ◽  
Vol 228 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Thomas Pratt ◽  
Linda Sharp ◽  
Jenny Nichols ◽  
David J. Price ◽  
John O. Mason
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Embryonic Stem Cells ◽  
Transgenic Mice ◽  
Fluorescent Protein ◽  
Embryonic Stem ◽  
Green Fluorescent

Expression of green fluorescent protein under the regulation of human locus control region elements HS2 and HS3 in transgenic mice

2008 ◽  
Vol 88 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Jingbin Yan ◽  
Yanping Xiao ◽  
Shu Wang ◽  
Zhijuan Gong ◽  
Shuzheng Huang ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Control Region ◽  
Fluorescent Protein ◽  
Locus Control Region ◽  
Green Fluorescent

Heterogeneity in histone 2B-green fluorescent protein-retaining putative small intestinal stem cells at cell position 4 and their absence in the colon

2012 ◽  
Vol 303 (11) ◽  
pp. G1188-G1201 ◽  
Author(s):  
Kevin R. Hughes ◽  
Ricardo M. C. Gândara ◽  
Tanvi Javkar ◽  
Fred Sablitzky ◽  
Hanno Hock ◽  
...  
Keyword(s):  
Stem Cells ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Paneth Cells ◽  
Epithelial Stem Cells ◽  
Epithelial Regeneration ◽  
Cell Position ◽  
Small Intestinal ◽  
Green Fluorescent ◽  
Dose Radiation

Stem cells have been identified in two locations in small intestinal crypts; those intercalated between Paneth cells and another population (which retains DNA label) are located above the Paneth cell zone, at cell position 4. Because of disadvantages associated with the use of DNA label, doxycycline-induced transient transgenic expression of histone 2B (H2B)-green fluorescent protein (GFP) was investigated. H2B-GFP-retaining putative stem cells were consistently seen, with a peak at cell position 4, over chase periods of up to 112 days. After a 28-day chase, a subpopulation of the H2B-GFP-retaining cells was cycling, but the slow cycling status of the majority was illustrated by lack of expression of pHistone H3 and Ki67. Although some H2B-GFP-retaining cells were sensitive to low-dose radiation, the majority was resistant to low- and high-dose radiation-induced cell death, and a proportion of the surviving cells proliferated during subsequent epithelial regeneration. Long-term retention of H2B-GFP in a subpopulation of small intestinal Paneth cells was also seen, implying that they are long lived. In contrast to the small intestine, H2B-GFP-retaining epithelial cells were not seen in the colon from 28-day chase onward. This implies important differences in stem cell function between these two regions of the gastrointestinal tract, which may have implications for region-specific susceptibility to diseases (such as cancer and ulcerative colitis), in which epithelial stem cells and their progeny are involved.

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