scholarly journals Evaluation of Chemical Preparation on Insect Wing Shape for Geometric Morphometrics

2013 ◽  
Vol 89 (5) ◽  
pp. 928-931 ◽  
Author(s):  
Camila Lorenz ◽  
Lincoln Suesdek
Keyword(s):  
Geometric Morphometrics ◽  
Wing Shape ◽  
Chemical Preparation ◽  
Insect Wing

scholarly journals Bmmp influences wing shape by regulating anterior-posterior and proximal-distal axis development

2021 ◽  
Author(s):  
Yunlong Zou ◽  
Xin Ding ◽  
Li Zhang ◽  
Lifeng Xu ◽  
Shubo Liang ◽  
...  
Keyword(s):  
Molecular Mapping ◽  
Wing Shape ◽  
Wing Development ◽  
Mrna Levels ◽  
Nucleotide Polymorphisms ◽  
Insect Wing ◽  
Expression Of Genes ◽  
Flight Capacity ◽  
Null Mutants ◽  
Anterior Posterior

Insect wings are subject to strong selective pressure, resulting in the evolution of remarkably diverse wing shapes that largely determine flight capacity. However, the genetic basis and regulatory mechanisms underlying wing shape development are not well understood. The silkworm Bombyx mori micropterous ( mp ) mutant exhibits shortened wing length and enlarged vein spacings , albeit without changes in total wing area. Thus, the mp mutant comprises a valuable genetic resource for studying wing shape development. In this study, we used molecular mapping to identify the gene responsible for the mp phenotype and designated it Bmmp . Phenotype-causing mutations were identified as indels and single nucleotide polymorphisms in non-coding regions. These mutations resulted in decreased Bmmp mRNA levels and changes in transcript isoform composition. Bmmp null mutants were generated by CRISPR/Cas9 and exhibited significantly smaller wings. By examining the expression of genes critical to wing development in wildtype and Bmmp null mutants, we found that Bm mp exerts its function by coordinately modulating anterior-posterior and proximal-distal axis development. We also studied a Drosophila mp mutant and found that Bmmp is functionally conserved in Drosophila . The Drosophila mp mutant strain exhibits curly wings of reduced size and a complete loss of flight capacity. Our results increase our understanding of the mechanisms underpinning insect wing development and reveal potential targets for pest control.

Intraspecific variation in wing shape of Poecilobothrus regalis (Meigen, 1824) (Diptera, Dolichopodidae)

2016 ◽  
Vol 4 (16) ◽  
pp. 1
Author(s):  
Mariya A. Chursina ◽  
Oleg P. Negrobov
Keyword(s):  
Geometric Morphometrics ◽  
Flow Velocity ◽  
Intraspecific Variability ◽  
Wing Shape ◽  
Canonical Variate ◽  
Wind Flow ◽  
Allometric Relationships ◽  
Shape Variation ◽  
Average Temperature ◽  
Average Wind

A study of 186 specimens of Poecilobothrus regalis was conducted in order to examine intraspecific variability of wing shape. The wing shape variation was analyzed using geometric morphometrics analyses. Significant differences in the structure of wing were found both between sexes and between populations. Differences between sexes were observed in the structure of the medium portion of wing. The first extracted canonical variate of geographic variation showed a moderately linear association with latitude and average temperature of February and March. The second canonical variate was correlated with longitude and values of average wind flow velocity. Allometric relationships were weak both between populations and sexes.

scholarly journals Quantifying Wing Shape and Size of Saturniid Moths with Geometric Morphometrics

2016 ◽  
Vol 70 (2) ◽  
pp. 99-107 ◽  
Author(s):  
Minjia Zhong ◽  
Geena M. Hill ◽  
Juan P. Gomez ◽  
David Plotkin ◽  
Jesse R. Barber ◽  
...  
Keyword(s):  
Geometric Morphometrics ◽  
Wing Shape ◽  
Shape And Size

scholarly journals Differentiation of Five Species of Megachile (Hymenoptera: Megachilidae), based on Wing Shape

2016 ◽  
Vol 60 (2) ◽  
pp. 41-50 ◽  
Author(s):  
Shahram Falamarzi ◽  
Behzad Habibpour ◽  
Mohammad S. Mossadegh ◽  
Alireza Monfared
Keyword(s):  
Geometric Morphometrics ◽  
Cross Validation ◽  
Principal Component ◽  
Wing Shape ◽  
Canonical Variate ◽  
Effective Technique ◽  
Validation Test ◽  
Variate Analysis ◽  
Clear Differentiation ◽  
Respective Species

Abstract In the present work we used landmark-based geometric morphometrics to compare the wing shapes of five species of Megachile (belonging to three subgenera) to confirm whether this technique may be used reliably for differentiation of this group. Analyses of wing shape by the use of principal component analysis (PCA), and canonical variate analysis (CVA) led to a clear differentiation among species. We found a close phenotypic similarity in wing shape between M. albisecta (belonging to the subgenus Creightonella) and M. picicornis (belonging to the subgenus Eutricharaea). According to the results of UPGMA, a higher degree of divergence between M. farinosa (belonging to the subgenus Pseudomegachile) and species belonging to other subgenera, was detected. The results of a cross-validation test indicated that geometric morphometrics is an effective technique to use for distinguishing between Megachile species. The reliability rate of this technique was between 85.71-100%. Using only two submarginal cell landmarks for generating shape variables, the cross-validation test correctly assigned individuals to their respective species, with a 92.85-100% reliability rate. Significant differences in wing size were obtained among the analysed species.

scholarly journals Molecular Identification and Geometric Morphometric Analysis of Haematobosca aberrans (Diptera: Muscidae)

Insects ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 451 ◽  
Author(s):  
Tanasak Changbunjong ◽  
Jiraporn Ruangsittichai ◽  
Gerard Duvallet ◽  
Adrian C. Pont
Keyword(s):  
Sexual Dimorphism ◽  
Geometric Morphometrics ◽  
Morphometric Analysis ◽  
Morphological Characters ◽  
Wing Shape ◽  
Coi Gene ◽  
Morphological Identification ◽  
Data Systems ◽  
Geometric Morphometric ◽  
Geometric Morphometric Analysis

The genus Haematobosca Bezzi, 1907 (Diptera: Muscidae) contains haematophagous flies of veterinary importance. A new fly species of this genus was recognised from northern Thailand based on morphological characters and described as Haematobosca aberrans Pont, Duvallet & Changbunjong, 2020. In the present study, the mitochondrial cytochrome c oxidase I (COI) gene was used to confirm the morphological identification of H. aberrans. In addition, landmark-based geometric morphometrics was used to determine sexual dimorphism. The molecular analysis was conducted with 10 COI sequences. The results showed that all sequences were 100% identical. The sequence was not highly similar to reference sequences from GenBank and did not match any identified species from Barcode of Life Data Systems (BOLD). Phylogenetic analysis clearly differentiated this species from other species within the subfamily Stomoxyinae. For geometric morphometric analysis, a total of 16 wing pictures were analysed using the landmark-based approach. The results showed significant differences in wing shape between males and females, with a cross-validated classification score of 100%. The allometric analysis showed that wing shape has no correlation with size. Therefore, the COI gene is effective in species identification of H. aberrans, and geometric morphometrics is also effective in determining sexual dimorphism.

scholarly journals Identifying of Homoneura (Diptera: Lauxaniidae) females by means of geometric morphometrics

2017 ◽  
Vol 66 (1) ◽  
pp. 75-86
Author(s):  
Marek Semelbauer
Keyword(s):  
Geometric Morphometrics ◽  
Principal Component ◽  
Wing Shape ◽  
Procrustes Analysis ◽  
Distribution Data ◽  
Multivariate Methods ◽  
Linear Discriminant ◽  
European Species ◽  
Generalized Procrustes Analysis ◽  
Males And Females

Abstract The females of the European species Homoneura consobrina, H. patelliformis and H. thalhammeri are not easy to discern. A possible method of identifying females is to apply geometric morphometrics to the wing shape, when we expect females of certain species to have wing shape more similar to conspecific males, than to other species. For this purpose, 94 specimens of the three species were collected mainly by means of Malaise trap in western and central Slovakia and Montenegro. The combination of morphological and distribution data allowed for the females to be divided into three groups. In next step, wings of both males and females were digitized, aligned by Generalized Procrustes Analysis and analyzed via multivariate methods (principal component analysis, multivariate analysis of variance, linear discriminant analysis). Results of the analysis clearly suggest that females with large and convex 8th sternite belong to H. patelliformis, while females with reduced 8th sternite belong to either H. thalhammeri or H. consobrina.

scholarly journals Approach to insect wing shape and deformation field measurement

2017 ◽  
Author(s):  
Duo Yin ◽  
Zhen Wei ◽  
Zeyu Wang
Keyword(s):  
Field Measurement ◽  
Local Deformation ◽  
Wing Shape ◽  
Deformation Field ◽  
Concentrated Force ◽  
Insect Wing ◽  
Summary Statement ◽  
Static Criterion ◽  
Stiffness Distribution ◽  
Better Than

Summary StatementA fine shape and deformation field measurement of insect wing is achieved by a self-developed setup. This measurement could foster investigation of insect wing stiffness distribution.AbstractFor measuring the shape and deformation of insect wing, a scanning setup adopting line laser and coaxial LED light is developed. Wing shape can be directly acquired from the line laser images by triangulation. Yet the wing deformation field can also be obtained by a self-devised algorithm that processes the images from line laser and coaxial LED simultaneously. During the experiment, three wing samples from termite and mosquito under concentrated force are scanned. The venation and corrugation could be significantly identified from shape measurement result. The deformation field is sufficiently accurate to demonstrate its variation from wing base to tip. The load conditions in experiments are also be discussed. For softer wings, local deformation is apparent if pinhead is employed to impose force. The similarity analysis is better than 5% deformation ratio as a static criterion, if the wing is simplified as a cantilever beam. The setup is proved to be effective and versatile. The shape and deformation fields would give enough details for the measurement of wing stiffness distribution.

Taxonomic affinity of halictid bee fossils (Hymenoptera: Anthophila) based on geometric morphometrics analyses of wing shape

2012 ◽  
Vol 10 (4) ◽  
pp. 755-764 ◽  
Author(s):  
Thibaut De Meulemeester ◽  
Denis Michez ◽  
Ahmet Murat Aytekin ◽  
Bryan Nicholas Danforth
Keyword(s):  
Geometric Morphometrics ◽  
Wing Shape ◽  
Taxonomic Affinity

scholarly journals Identification of the species of the Cheilosia variabilis group (Diptera, Syrphidae) from the Balkan Peninsula using wing geometric morphometrics, with the revision of status of C. melanopa redi Vujić, 1996

2009 ◽  
Vol 78 (3) ◽  
pp. 129-140 ◽  
Author(s):  
Lj. Francuski ◽  
A. Vujić ◽  
A. Kovačević ◽  
J. Ludoški ◽  
V. Milankov
Keyword(s):  
Geometric Morphometrics ◽  
Balkan Peninsula ◽  
Wing Shape ◽  
Canonical Variate ◽  
Species Boundaries ◽  
Wing Size ◽  
Mahalanobis Distances ◽  
Males And Females ◽  
Wing Geometric Morphometrics ◽  
Shape And Size

The present study investigates phenotypic differentiation patterns among four species of the Cheilosia variabilis group (Diptera, Syrphidae) using a landmark-based geometric morphometric approach. Herein, wing geometric morphometrics established species boundaries that confirm C. melanopa and C. redi stat. nov. as evolutionarily independent entities or separate species within the previously described C. melanopa species. Seventeen samples of C. lasiopa, C. variabilis, C. melanopa and C. redi from 11 localities on the Balkan Peninsula were analysed. Based on wing shape and size, both species boundaries and intraspecific divergent units were delineated indicating the possible presence of evolutionarily independent units within the analysed taxa. Significant differences in wing size were obtained among the analysed species and canonical variate analysis showed that wing shape was sufficiently different to allow the correct classification of 99.7% and 100% individuals of males and females, respectively. Considerable wing size variation was detected among males and females of conspecific populations of C. melanopa and males of C. variabilis. Contrary to partial discrimination based on the male wing shape among conspecific populations of C. redi and C. variabilis, clear separation of conspecific populations of C. melanopa (for both sexes) and females of C. redi and C. variabilis was observed. The UPGMA cluster analysis based on squared Mahalanobis distances revealed a close similarity between C. melanopa and C. redi, whereas C. lasiopa was the most divergent species. Results presented in this study utilize wing shape and size as new taxonomic characters in delimitating the closely related species and populations of the Cheilosia variabilis group.

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