Human Visceral Leishmaniasis: Analysis of the Specificity of Humoral Immune Response to Polypeptides of Leishmania Donovani Chagasi

40 blood sample were taken from children infected with kala-azar at age less than ten years, who were admitted to El-Eskan child hospital, and central health laboratory in Baghdad. At the same time 15 sample collected from healthy child for comparing. During research estimated immunoglobulins level ( IgG, IgM, IgA) and (IFAT) IFAT test recorded a higher indicate antibodies level witch was synergistic with infected severity, the stander level of antibodies arrange between 1/16, 1/32 and 1/64 that means it graduate from the lowest one 1/16 into the highest one 1/64 within groups. During infectious the IgG ,IgM level recorded the higher level (2012.9 mg/dl) and (154.3 mg/dl) compared to the control group (874.3 mg/dl), (91.4 mg/dl) while there no significant differences in IgA level between patients group( 143.8 mg/dl ) And healthy control group (116.2 mg/dl) .

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Immunoblot Analysis of the Humoral Immune Response to Leishmania donovani Polypeptides in Cases of Human Visceral Leishmaniasis: Its Usefulness in Prognosis

Clinical and Vaccine Immunology ◽

10.1128/cdli.9.5.1119-1123.2002 ◽

2002 ◽

Vol 9 (5) ◽

pp. 1119-1123 ◽

Cited By ~ 2

Author(s):

Promod Kumar ◽

Kalpana Pai ◽

Kiran Tripathi ◽

H. P. Pandey ◽

Shyam Sundar

Keyword(s):

Visceral Leishmaniasis ◽

Leishmania Donovani ◽

Immunoblot Analysis ◽

Specific Pattern ◽

Sensitive Test ◽

Soluble Extract ◽

Serum Samples ◽

Humoral Immune ◽

Human Visceral Leishmaniasis ◽

Sodium Antimony Gluconate

ABSTRACT Sera from Indian patients with parasitologically confirmed visceral leishmaniasis were studied by immunoblot analysis in order to identify a specific pattern for Leishmania infection. A soluble extract of Leishmania donovani was used as antigen. At diagnosis the sera from patients with visceral leishmaniasis specifically recognized fractions represented by bands of 201 kDa (50% of serum samples), 193 kDa (60%), 147 kDa (50%), 120 kDa (60%), 100 kDa (50%), 80 kDa (80%), 70 kDa (70%), 65 kDa (100%), 50 kDa (50%), 36 kDa (50%), 20 kDa (70%), and 18 kDa (50%). The 65-kDa band, common to all patients infected with Leishmania parasites, was found at the time of diagnosis. However, the immunoblot pattern changed after patients were treated and cured with sodium antimony gluconate (SAG; n =10) or miltefosine (n =10), as was evident from blots of sera obtained pretreatment and at 1, 3, and 6 months posttreatment. At 6 months posttreatment, immunoblots of sera from patients on the SAG regimen showed the disappearance of all bands except the 70-kDa band. Similarly, sera from those on the miltefosine regimen showed the disappearance of all bands except the 65- and 70-kDa bands. This study shows that Western blot analysis is a sensitive test for detection of anti-Leishmania antibodies. Moreover, the persistence of reactivity with the 65- and 70-kDa bands in the sera of all groups shows its promise as a diagnostic and prognostic tool.

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