Engineered resistance in Aedes aegypti to a West African and a South American strain of yellow fever virus.

1998 ◽  
Vol 58 (5) ◽  
pp. 663-670 ◽  
Author(s):  
S Higgs ◽  
K E Olson ◽  
B S Davis ◽  
B J Beaty ◽  
C D Blair ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Yellow Fever Virus ◽  
West African ◽  
Fever Virus ◽  
South American ◽  
American Strain ◽  
Engineered Resistance

scholarly journals Genetic Relationships and Evolution of Genotypes of Yellow Fever Virus and Other Members of the Yellow Fever Virus Group within the Flavivirus Genus Based on the 3′ Noncoding Region

2004 ◽  
Vol 78 (18) ◽  
pp. 9652-9665 ◽  
Author(s):  
John-Paul Mutebi ◽  
René C. A. Rijnbrand ◽  
Heiman Wang ◽  
Kate D. Ryman ◽  
Eryu Wang ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Yellow Fever ◽  
Yellow Fever Virus ◽  
Genetic Relationships ◽  
West African ◽  
Fever Virus ◽  
Genetic Group ◽  
Noncoding Region ◽  
South American ◽  
Sequence Elements

ABSTRACT Genetic relationships among flaviviruses within the yellow fever (YF) virus genetic group were investigated by comparing nucleotide sequences of the 3′ noncoding region (3′NCR). Size heterogeneity was observed between members and even among strains of the same viral species. Size variation between YF strains was due to duplications and/or deletions of repeated nucleotide sequence elements (RYF). West African genotypes had three copies of the RYF (RYF1, RYF2, and RYF3); the Angola and the East and Central African genotypes had two copies (RYF1 and RYF3); and South American genotypes had only a single copy (RYF3). Nucleotide sequence analyses suggest a deletion within the 3′NCR of South American genotypes, including RYF1 and RYF2. Based on studies with the French neurotropic vaccine strain, passage of a YF virus strain in cell culture can result in deletion of RYF1 and RYF2. Taken together, these observations suggest that South American genotypes of YF virus evolved from West African genotypes and that the South American genotypes lost RYF1 and RYF2, possibly in a single event. Repeated sequence elements were found within the 3′NCR of other members of the YF virus genetic group, suggesting that it is probably characteristic for members of the YF virus genetic group. A core sequence of 15 nucleotides, containing two stem-loops, was found within the 3′NCR of all members of the YF genetic group and may represent the progenitor repeat sequence. Secondary structure predictions of the 3′NCR showed very similar structures for viruses that were closely related phylogenetically.

scholarly journals Genome analysis and phylogenetic relationships between east, central and west African isolates of Yellow fever virus

2006 ◽  
Vol 87 (4) ◽  
pp. 895-907 ◽  
Author(s):  
Jana J. von Lindern ◽  
Sarah Aroner ◽  
Nicholas D. Barrett ◽  
Jason A. Wicker ◽  
C. Todd Davis ◽  
...  
Keyword(s):  
Amino Acid ◽  
Yellow Fever ◽  
Genome Analysis ◽  
Yellow Fever Virus ◽  
Prototype Strain ◽  
West African ◽  
Fever Virus ◽  
Full Genome ◽  
Genome Database ◽  
East Central

Yellow fever virus (YFV), a reemerging disease agent in Africa and South America, is the prototype member of the genus Flavivirus. Based on examination of the prM/M, E and 3′ non-coding regions of the YFV genome, previous studies have identified seven genotypes of YFV, including the Angolan, east/central African and east African genotypes, which are highly divergent from the prototype strain Asibi. In this study, full genome analysis was used to expand upon these genetic relationships as well as on the very limited full genome database for YFV. This study was the first to investigate genomic sequences of YFV strains from east and central Africa (Angola71, Uganda48a and Ethiopia61b). All three viruses had genomes of 10 823 nt in length. Compared with the prototype strain Asibi (from west Africa) they were approximately 25 % divergent in nucleotide sequence and 7 % divergent in amino acid sequence. Comparison of multiple flaviviruses in the N-terminal region of NS4B showed that amino acid sequences were variable and that west African strains of YFV had an amino acid deletion at residue 21. Additionally, N-linked glycosylation sites were conserved between viral genotypes, while codon usage varied between strains.

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