scholarly journals Coagulase negative staphylococci in Anti-Cancer Center, Batna, Algeria: antibiotic resistance pattern, biofilm formation, and detection of mecA and icaAD genes

2019 ◽  
Vol 21 (1) ◽  
pp. 21
Author(s):  
A. Zatout ◽  
R. Djibaoui ◽  
A. Kassah-Laouar ◽  
C. Benbrahim
Keyword(s):  
Antibiotic Resistance ◽  
Biofilm Formation ◽  
Resistance Pattern ◽  
Cancer Center ◽  
Coagulase Negative Staphylococci ◽  
Antibiotic Resistance Pattern ◽  
Anti Cancer

scholarly journals Plasmid analysis as an epidemiological tool in neurosurgical infections with coagulase-negative staphylococci

1987 ◽  
Vol 98 (3) ◽  
pp. 231-239 ◽  
Author(s):  
Åke Örtqvist ◽  
Ulrika Ransjö ◽  
Bengt Wretlind
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Pattern ◽  
Enzyme Analysis ◽  
Restriction Enzyme Analysis ◽  
Coagulase Negative Staphylococci ◽  
Antibiotic Resistance Pattern ◽  
Plasmid Analysis ◽  
Epidemiological Tool ◽  
Resistance To Penicillin ◽  
Different Strains

SUMMARYCoagulase-negative staphylococci isolated from blood or spinal fluid during a period of 1 year in a department of neurosurgery, were analysed by biotyping, antibiotic resistance pattern and plasmid profiles. Altogether 41 isolates from 19 patients were studied. About 90% of the isolates wereStaphylococcus epidermidis. The antibiotic resistance pattern seemed to be closely related to antibiotic usage in the unit. Most common was resistance to penicillin (63%), trimethoprimsulphamethoxazole (49%) and cloxacillin (39%) while resistance to gentamicin was seen in only one strain.In several cases species and antibiograms were identical in isolates from different patients. Plasmid pattern analysis could then be used for identification of different strains. In one instance, plasmid pattern and restriction enzyme analysis confirmed that two patients probably were infected by the same strain.

Biofilm formation and antibiotic resistance pattern of dominantStaphylococcus aureusclonal lineages in China

2016 ◽  
Vol 37 (2) ◽  
pp. e12304 ◽  
Author(s):  
Minghui Song ◽  
Qiongqiong Li ◽  
Yi Zhang ◽  
Jinxia Song ◽  
Xianming Shi ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Biofilm Formation ◽  
Resistance Pattern ◽  
Antibiotic Resistance Pattern

scholarly journals Study of Antibiotic Resistance Pattern in Coagulase-negative Staphylococci strains Isolated from Clinical Specimens

2018 ◽  
Vol 25 (2) ◽  
pp. 85-91
Author(s):  
Yousef Nami ◽  
Mehdi Ghiami Rad ◽  
Hamid Farah Bakhsh ◽  
Nazila Imeni ◽  
◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Pattern ◽  
Coagulase Negative Staphylococci ◽  
Clinical Specimens ◽  
Antibiotic Resistance Pattern

scholarly journals A Cross-Sectional Study on Association between Antibiotic Resistance Pattern and Biofilm Production of E. coli in NonCatheterised UTI Patients at a Tertiary Care Hospital in Kolkata

2021 ◽  
Vol 8 (10) ◽  
pp. 542-546
Author(s):  
Debalina Das ◽  
Parthasarathi Chakrabarty ◽  
Sipra Saha ◽  
Nandita Pal ◽  
Susmita Bhattacharya
Keyword(s):  
Antibiotic Resistance ◽  
Congo Red ◽  
Biofilm Formation ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Resistance Pattern ◽  
Care Hospital ◽  
E Coli ◽  
Antibiotic Resistance Pattern ◽  
Biofilm Production

BACKGROUND Urinary tract infections are some of the most common community-acquired as well as nosocomial infections with E. coli being the most common pathogen. There is increased antimicrobial resistance among bacteria worldwide. One of the important mechanisms of resistance and virulence of bacteria is biofilm formation. This study was conducted to find out the association between antibiotic resistance pattern and biofilm formation in E. coli in non-catheterised patients of UTI in a tertiary care hospital. We further wanted to determine the association between the ability of E. coli to form biofilm and their ability to produce extended-spectrum beta-lactamases (ESBLs) and carbapenemase in non-catheterised patients. METHODS Urine samples collected from 300 non-catheterised patients who had symptoms of UTI were inoculated into MacConkey’s agar and blood agar media. Then identification and antibiotic susceptibility tests were done. Phenotypic detection of ESBL production was done by double disc diffusion test and carbapenemase production was done by mCIM (modified carbapenem inactivation method) and eCIM (EDTA carbapenem inactivation method) tests according to Clinical and Laboratory Standards Institute (CLSI) 2019 guideline. Biofilm detection was done by Congo red agar (CRA) method. RESULTS Out of 78 isolates E. coli were the commonest (61.5 %) isolate. Out of 48 E. coli isolates from non-catheterised UTI patients, 26 (54.1%) were biofilm producers. Antibiotic sensitivity pattern among the E. coli isolates showed the highest susceptibility of the strains to amikacin, whereas the least susceptibility was for amoxicillin. Out of 48 E. coli, 20 (41.6 %) were ESBL producers, 16 (33.33 %) E. coli were carbapenemase producers. Significant association was found between ESBL and biofilm production. However, no statistical significance was found between the association of carbapenemase production and biofilm formation. CONCLUSIONS Uropathogenic E. coli is not an uncommon pathogen for biofilm formation even in non-catheterised patients. The antibiotic-resistance rate was higher among biofilm producing E. coli isolates. The biofilm forming ability was found to be significantly higher among ESBL producing strains but was not statistically significant for carbapenemase producing strains of E. coli. KEYWORDS Biofilm, Uropathogen, Congo Red Agar (CRA) Method, UTI, ESBL, Carbapenemase

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