scholarly journals Modifying and adapting a plant-based DNA extraction protocol for human genomic DNA extraction: a cost effective approach

2017 ◽  
Vol 23 (1) ◽  
pp. 1
Author(s):  
M.E. Kooffreh ◽  
O.U. Udensi ◽  
A.J. Umoeyen
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Cost Effective ◽  
Genomic Dna Extraction ◽  
Extraction Protocol ◽  
Cost Effective Approach ◽  
Human Genomic ◽  
Human Genomic Dna

scholarly journals An Efficiency Human Genomic DNA Extraction from Dried Blood Spots

2011 ◽  
Vol 8 ◽  
pp. 179-185 ◽  
Author(s):  
Nguyen Thi Hue ◽  
Phan Tuan Phong ◽  
Nguyen Dieu Hoai Chan ◽  
Nguyen Khac Han Hoan ◽  
Huynh Thi Thu Thuy
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Dried Blood Spots ◽  
Genomic Dna Extraction ◽  
Blood Spots ◽  
Human Genomic ◽  
Human Genomic Dna

scholarly journals A simplified genomic DNA extraction protocol for pre-germination genotyping in rice

2015 ◽  
Vol 14 (2) ◽  
pp. 6369-6375 ◽  
Author(s):  
Y.B. Duan ◽  
F.L. Zhao ◽  
H.D. Chen ◽  
H. Li ◽  
D.H. Ni ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Genomic Dna Extraction ◽  
Extraction Protocol

A systematic evaluation of stool DNA preparation protocols for colorectal cancer screening via analysis of DNA methylation biomarkers

2021 ◽  
Vol 59 (1) ◽  
pp. 91-99
Author(s):  
Shengnan Jin ◽  
Qian Ye ◽  
Yanping Hong ◽  
Wenqing Dai ◽  
Chengliang Zhang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Dna Methylation ◽  
Dna Extraction ◽  
Genomic Dna ◽  
Magnetic Beads ◽  
Systematic Evaluation ◽  
Stool Samples ◽  
Human Genomic ◽  
Tumor Dna ◽  
Human Genomic Dna

AbstractObjectivesColorectal cancer (CRC) screening using stool samples is now in routine use where tumor DNA methylation analysis for leading markers such as NDRG4 and SDC2 is an integral part of the test. However, processing stool samples for reproducible and efficient extraction of human genomic DNA remains a bottleneck for further research into better biomarkers and assays.MethodsWe systematically evaluated several factors involved in the processing of stool samples and extraction of DNA. These factors include: stool processing (solid and homogenized samples), preparation of DNA from supernatant and pellets, and DNA extraction with column and magnetic beads-based methods. Furthermore, SDC2 and NDRG4 methylation levels were used to evaluate the clinical performance of the optimal protocol.ResultsThe yield of total and human genomic DNA (hgDNA) was not reproducible when solid stool scraping is used, possibly due to sampling variations. More reproducible results were obtained from homogenized stool samples. Magnetic beads-based DNA extraction using the supernatant from the homogenized stool was chosen for further analysis due to better reproducibility, higher hgDNA yield, lower non-hgDNA background, and the potential for automation. With this protocol, a combination of SDC2 and NDRG4 methylation signals with a linear regression model achieved a sensitivity and specificity of 81.82 and 93.75%, respectively.ConclusionsThrough the systematic evaluation of different stool processing and DNA extraction methods, we established a reproducible protocol for analyzing tumor DNA methylation markers in stool samples for colorectal cancer screening.

scholarly journals Establishment of a genomic DNA extraction protocol for bamboo species

2018 ◽  
Vol 41 (3) ◽  
pp. 825-831
Author(s):  
Rudieli M. Silva ◽  
Nathalia P. Ribeiro
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Bamboo Species ◽  
Genomic Dna Extraction ◽  
Extraction Protocol

scholarly journals Optimization of genomic DNA extraction protocol for black wattle

Agrarian ◽  
2020 ◽  
Vol 13 (49) ◽  
pp. 323-329
Author(s):  
Yasmin Imparato Maximo ◽  
Angela Cristina Ikeda ◽  
Paulo César Flôres Júnior ◽  
Giovana Bomfim De Alcantara ◽  
Antonio Higa
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Proteinase K ◽  
Genomic Dna Extraction ◽  
Extraction Protocol ◽  
Black Wattle

Considerando-se que a atual tendência do melhoramento florestal é a integração das técnicas clássicas com as de análise genética molecular, faz-se necessária a obtenção de protocolos de extração de DNA genômico ajustados a cada espécie estudada. O objetivo do trabalho foi determinar o efeito de diferentes adaptações no protocolo de extração de DNA genômico CTAB para acácia-negra. Foram testados diferentes componentes na fase de extração orgânica: clorofórmio, fenol e proteinase K, além da aplicação de RNase após a fase de precipitação e limpeza do DNA. Também, foi investigada a eficiência destes tratamentos em amostras de folíolos frescas ou armazenadas em baixa temperatura durante sete dias. Foi verificada a presença de DNA de todas as amostras submetidas à extração pelo protocolo de CTAB com os diferentes tratamentos. O tempo de armazenamento das amostras não influenciou na integridade do DNA, entretanto, foi possível observar que a adição de RNase melhorou a qualidade do DNA extraído. Deste modo, sugere-se a utilização do protocolo CTAB com uso de clorofórmio e RNase, com amostras frescas ou armazenadas em baixas temperaturas.

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