scholarly journals Inoculum production and inoculation of Gnetum africanum rooted cuttings using a range of mycorrhizal fungi

Author(s):  
EE Tambe Bechem ◽  
IJ Alexander
1998 ◽  
Vol 28 (1) ◽  
pp. 150-153
Author(s):  
J N Gemma ◽  
R E Koske ◽  
E M Roberts ◽  
S Hester

Rooted cuttings of Taxus times media var. densiformis Rehd. were inoculated with the arbuscular mycorrhizal fungi Gigaspora gigantea (Nicol. & Gerd.) Gerd. & Trappe or Glomus intraradices Schenck and Smith and grown for 9-15 months in a greenhouse. At the completion of the experiments, leaves of inoculated plants contained significantly more chlorophyll (1.3-4.1 times as much) than did noninoculated plants. In addition, mycorrhizal plants had root systems that were significantly larger (1.3-1.4 times) and longer (1.7-2.1 times) than nonmycorrhizal plants, and they possessed significantly more branch roots (1.3-2.9 times). No differences in stem diameter and height or shoot dry weight were evident at the end of the experiments, although the number of buds was significantly greater in the cuttings inoculated with G. intraradices after 15 months.


2017 ◽  
Vol 69 (2) ◽  
pp. 291-298 ◽  
Author(s):  
Abdellatif Essahibi ◽  
Laila Benhiba ◽  
Fouad Oussouf ◽  
Mohamed Babram ◽  
Cherki Ghoulam ◽  
...  

The present investigation was undertaken to improve the performance of carob cuttings in terms of adventitious roots formation and hardening using arbuscular mycorrhizal fungi (AMF). Softwood cuttings were treated with 5000 mg L-1 of indole-3-butyric acid (IBA) and kept noninoculated (Non-AM) or inoculated with Funneliformis mosseae (Fmo) alone or combined with Rhizophagus fasciculatus (Fmo+Rfa) or R. intraradices (Fmo+Rin) or both (Fmo+Rfa+Rin) and then maintained under mist conditions. After two months, rooted cuttings were transplanted on sterilized substrate and transferred to a hardening greenhouse for five months. Obtained results showed that inoculation of the rooting substrate with AMF substantially improved the percentage of rooted cuttings and the number of roots per cutting. The highest rooting (63.33%) and number of roots per cutting (11.67) were recorded in the presence of the complex of the three AMF strains (Fmo+Rfa+Rin). Moreover, all mycorrhizal-rooted cuttings survived transplantation and hardening shocks and showed the highest growth and physiological performances. Indeed, in the Fmo-Rfa-Rin-plantlets the gains in plant height and shoot and root dry weights were 95.6%, 55.1% and 76.9% respectively. Furthermore, stomatal conductance, total chlorophyll content, photochemical efficiency of PSII (Fv/Fm) and nutrient concentrations were higher in mycorrhizal plantlets than in non-AM ones. Thus, AMF substantially improved carob cuttings? performance in terms of rooting capacity and hardening efficiency, thereby increasing the potential of carob propagation by cuttings.


2001 ◽  
Vol 19 (1) ◽  
pp. 15-20 ◽  
Author(s):  
C.F. Scagel

Abstract The benefits from root colonization by mycorrhizal fungi are thought to be highest when colonization occurs as early as possible during plant growth. We assessed whether addition of VA mycorrhizal fungi (VAMF) inoculum into rooting medium during cutting propagation would increase the quantity of rooting and the quality of rooted cuttings for five different cultivars of miniature roses (Rosa spp.). Four weeks after cuttings were stuck, the number of cuttings with roots for two cultivars that normally take longer to root, increased with addition of VAMF inoculum into the rooting medium. The combination of hormone treatment (IBA and NAA) and VAMF inoculum in the rooting medium increased the number of rooted cuttings and the number of roots per cutting for three cultivars when compared to cuttings that only received hormone treatment. Increases in root initiation and root growth of cuttings rooted in medium containing VAMF inoculum were not always associated with increased levels of root colonization by VAM fungi. Our results indicate that although adding VAMF inoculum into the rooting medium does not always increase root initiation, in some cultivars the combination of VAMF inoculum and rooting hormones can increase root initiation and potentially increase the quality of rooted cutting produced.


2021 ◽  
Vol 10 (4) ◽  
pp. 343-353
Author(s):  
Anju TANWAR ◽  
Ajay SİNGH ◽  
Ashok AGGARWAL ◽  
Esha JANGRA ◽  
Sergio T. PİCHARDO

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