scholarly journals The stability study of myristyl dimethyl amine oxide as an amphoteric surfactant in strong oxidant media containing 5 % m/m sodium hypochlorite through measurement of decomposing rate using high performance liquid chromatography and two phase titration

2017 ◽  
Vol 9 (1S) ◽  
pp. 235
Author(s):  
A Gholami ◽  
M.H. Taghriri
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Sodium Hypochlorite ◽  
High Performance ◽  
Amine Oxide ◽  
Stability Study ◽  
Amphoteric Surfactant ◽  
Two Phase ◽  
Dimethyl Amine ◽  
The Stability

scholarly journals Stability of Ceftiofur Sodium and Cefquinome Sulphate in Intravenous Solutions

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Agnieszka Dołhań ◽  
Anna Jelińska ◽  
Marcelina Bębenek
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
High Performance ◽  
Room Temperature ◽  
Stability Study ◽  
Test Conditions ◽  
Intravenous Solution ◽  
The Stability ◽  
Ceftiofur Sodium

Stability of ceftiofur sodium and cefquinome sulphate in intravenous solutions was studied. Chromatographic separation and quantitative determination were performed by using a high-performance liquid chromatography with UV-DAD detection. During the stability study, poly(vinylchloride) minibags were filled with a solution containing 5 mg of ceftiofur sodium or cefquinome sulphate and diluted to 0.2 mg/mL with suitable intravenous solution depending on the test conditions. The solutions for the study were protected from light and stored at room temperature (22°C), refrigerated (6°C), frozen (−20°C) for 30 days, and then thawed at room temperature. A comparison of results obtained at 22°C and 6°C for the same intravenous solutions showed that temperature as well as components of solutions and their concentration had an influence on the stability of ceftiofur sodium and cefquinome sulphate. It was found that ceftiofur sodium and cefquinome sulphate dissolved in intravenous solutions used in this study may be stored at room temperature and at 6°C for up to 48 h.

Differential intestinal deconjugation of taurine and glycine bile acid N-acyl amidates in rats

1992 ◽  
Vol 262 (2) ◽  
pp. G351-G358
Author(s):  
R. Zhang ◽  
S. Barnes ◽  
R. B. Diasio
Keyword(s):  
High Performance Liquid Chromatography ◽  
Small Intestine ◽  
Liquid Chromatography ◽  
Bile Acid ◽  
Chenodeoxycholic Acid ◽  
High Performance ◽  
Biliary Fistula ◽  
The Difference ◽  
The Stability ◽  
Rat Bile

Mechanisms responsible for the difference in the relative amounts of taurine- and glycine-conjugated bile acid N-acyl amidates (Tau/Gly ratio) are not fully understood. In the present study, the stability of taurine- and glycine-conjugated bile acid N-acyl amidates during intestinal transit and absorption was examined to investigate the contribution of intestinal deconjugation to the Tau/Gly ratio in rat bile. Radiolabeled chenodeoxycholic acid (CDC) and its N-acyl amidates with glycine (CDC-Gly) or taurine (CDC-Tau) were introduced into the lumen of the upper small intestine in the biliary fistula rats, and radioactive metabolites in bile, blood, urine, and tissues were identified and quantitated by high-performance liquid chromatography. Results indicated that 1) extensive deconjugation of CDC-Gly occurs during intestinal absorption; 2) CDC-Tau is recovered in bile largely intact; and 3) newly synthesized CDC-Tau and CDC-Gly are formed in a ratio of less than 2:1 after administration of [14C]-CDC. In summary, the present study demonstrates that resistance of taurine-conjugated bile acid N-acyl amidates to hydrolysis in the intestine, rather than a difference in synthesis of taurine- and glycine-conjugated N-acyl amidates in liver, may account for the high Tau/Gly ratio in rat bile.

scholarly journals Estimation of Some Useful Drugs in Water, Urine, and Medical Formulations Following Conventional and Modified Dispersive Microextraction Coupled with HPLC-UV

2021 ◽  
pp. 11-33
Author(s):  
Mitali Sarkar ◽  
Shanku Denrah
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Extraction Efficiency ◽  
Sodium Dodecyl ◽  
Two Phase ◽  
Preconcentration Factor ◽  
Dispersive Microextraction ◽  
Dispersive Liquid Liquid Microextraction ◽  
Thermodynamic Feasibility

Dispersive Liquid-Liquid Microextraction (DLLME) coupled with high-performance liquid chromatography-ultraviolet spectroscopy was developed, as a fast and precise operation, for extractive recovery and estimation of two pharmaceuticals viz. moxifloxacin and galantamine, from water, urine, and medical formulations. The process was investigated for Extraction (ES) and Dispersive Solvent (DS) as well as pH, temperature, and salt concentration. Extraction was found effective using methanol (CH3OH), as the DS, employing 1,1,2,2-tetrachloroethane (C2H2Cl4) and chloroform (CHCl3), as the ES, for moxifloxacin and galantamine respectively. The optimum pH was found to be 6.9 for moxifloxacin and 10.2 for galantamine. Temperature and salt were found to have some influence on the extraction efficiency of moxifloxacin but insignificant for galantamine. An improvement of the operation in terms of the Extraction efficiency (ER %), Preconcentration Factor (PF), thermodynamic feasibility, and greenness were achieved during surfactant aided DLLME (SDS-DLLME), where anionic surfactant (Sodium Dodecyl Sulphate (SDS)) was employed and no DS was required. Interestingly, the volume requirement for ES was found less, compared to that in the conventional DLLME, without compromising the performance. Moreover, quantitative recovery of both the drugs was achieved using a single ES. Thus, mutual separation and simultaneous determination of moxifloxacin and galantamine may be designed. A two-phase separation with concomitant enrichment of the solute in the sediment phase occurred. The drugs in the sediment phase, on subsequent dilution with methanol, were determined using the High Performance Liquid Chromatography-Ultraviolet (HPLC-UV) system. The negative free energy changes for the operation indicated that the process was thermodynamically feasible. The process was found to be effective for the spiked recovery of the studied drugs from real samples viz, water, human urine, and commercial medical formulations.

scholarly journals EFFECT OF USING L-ASPARAGINASE IN REDUCING ACRYLAMIDE PERCENTAGE IN BISICUT

2016 ◽  
Vol 47 (4) ◽  
Author(s):  
Jebur & et al.
Keyword(s):  
High Performance Liquid Chromatography ◽  
Enzyme Activity ◽  
Liquid Chromatography ◽  
High Performance ◽  
Specific Activity ◽  
Control Sample ◽  
The Other ◽  
Enzyme Efficiency ◽  
Negative Effect ◽  
The Stability

This study was aimed to know the efficiency of partially purified L- asparaginase produced from local isolate from Erwinia spp. to reduce the percentage of acrylamide formed in Biscuit. Four types of biscuit from wheat flour were prepared (T1, T2, T3, T4),and T1 as control. High performance liquid chromatography technique was used to estimate acrylamide ratio in biscuit , Effect of enzyme addition  on flour chemical and rheological properties was studied, also dough behavior ,gluten percentage, water absorption and amylase enzyme activity was estimated. The results revealed  that  the  addition of  experimental asparaginase ( specific activity 20.5 unite mg-1 ) with 1% of flour weight lead to reduce in acrylamide formation in Biscuit  to 89 %  compared  to  control sample ( in absence of enzyme ) . Moreover, the addition of Asparagine to flour at 0.1 % of its weight, where L- asparaginase was available caused a negative effect on enzyme efficiency in reducing the acrylamide in biscuit. So the level of acrylamide was reduced to 57.7 %. In the other hand , the percentage of acryl amide in biscuit was increased to   233 % when the asparagine was added to mixture in absence of L- asparaginase .Addition of  the enzyme to flour have no effect on the percentage value of gluten but improved the  stability of dough .The  enzyme  addition also led to increase amylases activities.  Addition of experimental enzyme had no effect on quality and sensory evaluation of biscuit.

scholarly journals EVALUATION OF INNOVATED FORMULA OF BISACODYL SUPPOSITORY FOLLOWING THE DISSOLUTION PROFILE AND STABILITY DATA USING DEVELOPED HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD

2020 ◽  
Author(s):  
KAHTAN J HASSON ◽  
ESRAA G JABAR ◽  
IHAB I ALKHALIFA
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Alkaline Medium ◽  
High Performance ◽  
Inclusion Complexation ◽  
Stability Study ◽  
Dissolution Profile ◽  
Chromatography Method ◽  
Beta Cyclodextrin ◽  
Liquid Chromatography Method

Objective: Bisacodyl is a laxative drug used in the treatment of constipation, it is soluble in mineral acids, but it is practically insoluble in water. Therefore, it is very hard task to dissolve bisacodyl in alkaline medium so the objective of this study was the development of proper dissolution method for a new formulation of bisacodyl suppositories in a medium simulated to rectal region. Obviously, most of the bisacodyl suppositories preparation products will yield low percentages of dissolution in the alkaline medium of phosphate buffer pH 7.2. Methods: Preparation inclusion complex of bisacodyl with the solubilizing agent beta-cyclodextrin then incorporated in a suppository base. The quantitative analysis of bisacodyl in suppositories was carried by a developed and validated high-performance liquid chromatography method. Results and Discussion: The dissolution rates for the innovated formulation of bisacodyl complexed with beta-cyclodextrin suppositories were in average of 97.5% and the stored suppositories of this formulation maintained their specified physical and chemical properties along the real stability study. Conclusion: The application of the inclusion complexation technique of bisacodyl with beta-cyclodextrin in the production of suppositories enhances the dissolution rate and improves the stability of suppositories performance.

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