scholarly journals Distribution of Shewanella putrefaciens and Desulfovibrio vulgaris in sulphidogenic biofilms of industrial cooling water systems determined by fluorescent in situ hybridisation

Water SA ◽  
2002 ◽  
Vol 28 (2) ◽  
Author(s):  
Elise S McLeod ◽  
Raynard MacDonald ◽  
Volker S. Brozel
Keyword(s):  
In Situ Hybridisation ◽  
Cooling Water ◽  
Shewanella Putrefaciens ◽  
Water Systems ◽  
Desulfovibrio Vulgaris ◽  
Fluorescent In Situ Hybridisation

A detection method for Legionella spp in (cooling) water: fluorescent in situ hybridisation (FISH) on whole bacteria

2003 ◽  
Vol 47 (3) ◽  
pp. 143-146 ◽  
Author(s):  
P. Declerck ◽  
L. Verelst ◽  
L. Duvivier ◽  
A. Van Damme ◽  
F. Ollevier
Keyword(s):  
16S Rrna ◽  
In Situ Hybridisation ◽  
Cooling Water ◽  
Culture Methods ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Fluorescent In Situ Hybridisation ◽  
Background Staining ◽  
Legionella Species

Although traditional culture methods are appropriate for detection of Legionella species, such culture takes several days. Rapid detection (<24 h) of individual Legionella is possible using fluorescent in situ hybridisation (FISH) on whole bacteria. Water samples were filtered and the concentrated bacteria were immediately detected (without culture) with a fluorescence microscope following appropriate labelling. The detection level was very high and quantification was possible. For the detection of all Legionella spp. the probe LEG705 was used, complementary to a 16S rRNA sequence conserved in all Legionella spp. For specific detection of L. pneumophila the probe LEGPNE1 was used. This probe is designed against a variable domain of the 16S rRNA sequence from L. pneumophila. CY3 and FLUOS labels were tested and CY3 showed clearly detectable bacteria with minimum background staining. This FISH technique is very sensitive, fast, reliable and individual bacteria are easily detected.

Development of polymerase chain reaction and fluorescent in situ hybridisation techniques for the detection of a bacterial strain that degrades the cyanobacterial toxin microcystin LR

2005 ◽  
Vol 56 (8) ◽  
pp. 1127 ◽  
Author(s):  
D. G. Bourne ◽  
R. L. Blakeley ◽  
P. Riddles ◽  
G. J. Jones
Keyword(s):  
Polymerase Chain Reaction ◽  
Environmental Samples ◽  
In Situ Hybridisation ◽  
Cyanobacterial Blooms ◽  
Chain Reaction ◽  
Fluorescent In Situ Hybridisation ◽  
Cyanobacterial Toxin ◽  
Specific Amplification ◽  
Polymerase Chain

Polymerase chain reaction (PCR) and fluorescent in situ hybridisation (FISH) techniques were developed for the detection of a Sphingomonas bacterium (strain MJ-PV), previously demonstrated to degrade the cyanobacterial toxin microcystin LR. A PCR amplification protocol using the primer set Sph-f1008/Sph-r1243 demonstrated specific amplification of the target 16S ribosomal DNA (rDNA) of strain MJ-PV. A 16S ribosomal RNA (rRNA) targeted probe, Sph-r1264, labelled with a rhodamine fluorescent dye was successfully used in whole-cell FISH for the detection of MJ-PV in seeded controls. DNA primers and a PCR protocol were developed for the specific amplification of a gene, mlrA, which codes for the enzyme MlrA, responsible for hydrolysis of the cyanobacterial toxin microcystin LR. A survey using 16S rDNA and mlrA primers on extracted DNA from environmental samples of a lake that suffers regular toxic cyanobacterial blooms demonstrated no amplified products indicative of the presence of MJ-PV or mlrA. Although not detecting the MJ-PV strain in the tested environmental samples, these developed methods are useful to study the distribution of strain MJ-PV demonstrated to degrade mycrocystin LR in seeded bioremediation trails, as well as the distribution and the regulation of mlrA shown to be involved in mycrocystin LR degradation.

scholarly journals Unusual finding in the karyotype of a neonate with glandular hypospadias with chordee

2019 ◽  
Vol 12 (3) ◽  
pp. e228429
Author(s):  
Viraraghavan Vadakkencherry Ramaswamy ◽  
Gajanan Venkat Rao ◽  
Nori Suryanarayana ◽  
Sanghamitra Gummadapu
Keyword(s):  
Respiratory Distress ◽  
Y Chromosome ◽  
Clinical Finding ◽  
In Situ Hybridisation ◽  
Good Prognosis ◽  
Fluorescent In Situ Hybridisation ◽  
Parental Concerns ◽  
Unusual Finding

A 37-week, 2700 g vaginally delivered baby was admitted for respiratory distress which was attributed to transient tachypnoea of newborn. A clinical finding of glandular hypospadias with ventral chordee was detected. The penis was normal in size, and gonads were palpable bilaterally in the scrotal sac. The parents were informed of the good prognosis associated with this milder variety of hypospadias. In view of parental concerns, a fluorescent in situ hybridisation (FISH) for X and Y chromosome was performed. Surprisingly, FISH revealed the presence of 46, XY in 90% of cells and 46, XX in 10% of the remainder cells suggesting a diagnosis of chimerism.

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