Evaluation of an Enrichment-Plating Procedure for the Recovery of Campylobacter jejuni from Turkey Eggs and Meat

1982 ◽  
Vol 45 (14) ◽  
pp. 1276-1278 ◽  
Author(s):  
G. R. ACUFF ◽  
C. VANDERZANT ◽  
F. A. GARDNER ◽  
F. A. GOLAN
Keyword(s):  
Campylobacter Jejuni ◽  
Ferrous Sulfate ◽  
Antimicrobial Agents ◽  
Sodium Metabisulfite ◽  
Sodium Pyruvate ◽  
Enrichment Broth ◽  
Initial Cell ◽  
Selective Enrichment ◽  
Cell Numbers ◽  
Brucella Broth

A selective enrichment-plating procedure was tested for the recovery and enumeration of Campylobacter jejuni from turkey eggs and meat. Enrichment was in brucella broth with ferrous sulfate, sodium metabisulfite, sodium pyruvate and five antimicrobial agents. Plating was on brucella agar supplemented with equine blood and antimicrobial agents. Incubation of tubes and plates was at 42°C in an atmosphere of 5% O2:10% CO2:85% N2. C. jejuni could be recovered from the enrichment broth when calculated initial cell numbers per ml of broth were as low as 0.3 to 3.3

Selective enrichment broth medium for isolation of Campylobacter jejuni.

1983 ◽  
Vol 17 (5) ◽  
pp. 853-855 ◽  
Author(s):  
W T Martin ◽  
C M Patton ◽  
G K Morris ◽  
M E Potter ◽  
N D Puhr
Keyword(s):  
Campylobacter Jejuni ◽  
Enrichment Broth ◽  
Selective Enrichment ◽  
Selective Enrichment Broth

Campylobacter jejuni in Foods: Its Occurrence, Isolation from Foods, and Injury

1986 ◽  
Vol 49 (2) ◽  
pp. 161-166 ◽  
Author(s):  
SAMUEL A. PALUMBO
Keyword(s):  
Campylobacter Jejuni ◽  
Brilliant Green ◽  
Potassium Phosphate ◽  
Polymyxin B ◽  
Differential Count ◽  
Sodium Metabisulfite ◽  
Potassium Phosphate Buffer ◽  
Sodium Pyruvate ◽  
Heat Injury ◽  
Freeze Thaw

The following aspects of Campylobacter jejuni has been reviewed: characteristics of C. jejuni, its occurrence in foods, methods to quantitatively recover the organism from food, and heat injury and freeze-thaw stress of C. jejuni. C. jejuni can be heat injured in 0.1 M potassium phosphate buffer at 46°C. Heat injury can be demonstrated as the differential count between brucella agar plus ferrous sulfate, sodium metabisulfite and sodium pyruvate (FBP) and brilliant green 2% bile broth agar plus FBP. Heat-injured C. jejuni will grow on brucella agar containing either of the three antibiotic mixtures typically used to isolate C. jejuni. Heat-injured C. jejuni will repair (regain dye and bile tolerance) in brucella broth plus FBP. C. jejuni can be freeze-thaw stressed. This stress is demonstrated as a sensitivity to the antibiotic polymyxin B or incubation at 42°C. Addition of succinate and cysteine increased recovery of freeze-thaw stressed C. jejuni. Although the presence of injured/stressed C. jejuni in foods has not yet been detected, methods are now available to begin this search. The injury/stress process may explain the often encountered difficulty in isolating C. jejuni, especially low numbers, from foods.

Media for the Aerobic Resuscitation of Campylobacter jejuni

2007 ◽  
Vol 70 (5) ◽  
pp. 1099-1109 ◽  
Author(s):  
P. TANGWATCHARIN ◽  
S. CHANTHACHUM ◽  
P. KHOPAIBOOL ◽  
J. R. CHAMBERS ◽  
M. W. GRIFFITHS
Keyword(s):  
Campylobacter Jejuni ◽  
Lag Time ◽  
Exponential Phase ◽  
Sodium Metabisulfite ◽  
Aerobic Growth ◽  
Late Exponential Phase ◽  
Sodium Pyruvate ◽  
Time Saving ◽  
Initial Inoculum ◽  
Horse Blood

The microaerophilic nature of Campylobacter jejuni has complicated its recovery from human and animal sources. In this study, enhancement of the growth and aerotolerance of C. jejuni ATCC 35921 in nutrient broth no. 2 (NB2) was investigated. The efficiency of recovery of C. jejuni in NB2 containing FBP (0.025% [each] ferrous sulfate, sodium metabisulfite, and sodium pyruvate), 5% laked horse blood, hemin, Oxyrase, or activated charcoal in an aerobic atmosphere was compared with that obtained under microaerophilic incubation. The shortest lag time (λ) for cells grown aerobically was observed with NB2 supplemented with FBP, 5% laked horse blood, 0.01 g/liter of hemin, or 0.15 U/ml of Oxyrase. The efficacy of these media to resuscitate C. jejuni cells in late exponential phase, as well as cells subjected to stress induced by cold, heat, starvation, or acid, was determined in aerobic or microaerobic atmospheres. The λ of cells grown aerobically in NB2 containing both FBP and blood was similar to that obtained in the same medium incubated in a microaerobic environment (P > 0.05). However, the λ was longer during aerobic growth when low numbers of cells (approximately 1 log CFU/ml) in late exponential phase were used as the initial inoculum. The best recovery of stressed C. jejuni was observed in NB2 supplemented with FBP and blood and incubated aerobically. Enrichment in media incorporating FBP and 5% laked horse blood is a simple, convenient, and time-saving method to replace microaerophilic incubation methods for the resuscitation of C. jejuni.

Enumeration and Reduction of Campylobacter jejuni in Poultry and Red Meats

1985 ◽  
Vol 48 (7) ◽  
pp. 606-610 ◽  
Author(s):  
NORMAN J. STERN ◽  
PAUL J. ROTHENBERG ◽  
JACQUELINE M. STONE
Keyword(s):  
Campylobacter Jejuni ◽  
Meat Products ◽  
Ground Beef ◽  
Alternative Methods ◽  
Enrichment Broth ◽  
Direct Plating ◽  
Selective Enrichment ◽  
Freeze Thaw ◽  
Red Meats ◽  
Selective Enrichment Broth

This paper compares alternative methods for enumerating Campylobacter jejuni in meat products, the optimal levels of blood supplementation for enrichment recovery of the organism from chicken and ground beef, and means to reduce or eliminate Campylobacters from chicken through organic acid washes or freeze/thaw treatments. Direct plating onto Campy-BAP medium resulted in equivalent or greater recovery of C. jejuni, compared with MPN procedures using two media described in the literature. Subsequent studies employed direct plating, as it was also faster and simpler than the MPN procedures. Enumeration of enrichment cultures from a blood-containing selective enrichment broth indicated that optimal levels of blood supplementation for the recovery of Campylobacters was dependent on the food investigated. Not only did 0% blood supplementation enhance recovery of the organism from inoculated ground beef, but 7% supplementation actually decreased the numbers recovered from this product. The requirement for blood supplementation was reversed when C. jejuni recovery from chickens was assessed. Significant increases were seen when 7% supplementation was employed as compared with either 1 or 0% blood in the enrichment broth. A freeze (−15°C)/thaw treatment of chicken carcasses reduced the numbers of Campylobacter detected by a factor of greater than 100. Finally, 0.5% of either lactic or acetic acid washes of chicken carcasses at 50°C reduced the numbers of C. jejuni present.

Improved media for growth and aerotolerance of Campylobacter fetus

1978 ◽  
Vol 8 (1) ◽  
pp. 36-41
Author(s):  
H A George ◽  
P S Hoffman ◽  
R M Smibert ◽  
N R Krieg
Keyword(s):  
Sodium Metabisulfite ◽  
Growth Responses ◽  
Sodium Pyruvate ◽  
Brucella Broth ◽  
Campylobacter Fetus

The microaerophilic nature of Campylobacter fetus has complicated its recovery from human and animal sources. In this study, modifications of brucella agar and broth were tested for enhancement of growth and aerotolerance of 64 strains of C. fetus, representing each subspecies. Brucella agar supplemented with 0.025% each FeSO4 7H2O, sodium metabisulfite, and sodium pyruvate, supported growth of 98, 77, and 63% of the strains at 6% O2, 17% O2, and 21% O2, respectively. Unsupplemented brucella agar supported growth of 94, 48, and 20% of the strains. Brucella broth supplemented with 0.2% FeSO4.7H2O, 0.025% sodium metabisulfite, and 0.05% sodium pyruvate supported growth of 98% of the strains at 21% O2, compared to 75% with unsupplemented brucella broth. With both the supplemented agar and broth, growth responses occurred 1 to 2 days earlier than usual. Growth and aerotolerance of three strains of Campylobacter sputorum subsp. bubulus were not enhanced by the supplements.

Antimicrobial Resistance in Campylobacter jejuni from Broilers and Broiler House Environments in Norway

2007 ◽  
Vol 70 (3) ◽  
pp. 736-738 ◽  
Author(s):  
M. NORSTRÖM ◽  
G. JOHNSEN ◽  
M. HOFSHAGEN ◽  
H. THARALDSEN ◽  
H. KRUSE
Keyword(s):  
Antimicrobial Resistance ◽  
Campylobacter Jejuni ◽  
Antimicrobial Susceptibility ◽  
Antimicrobial Agents ◽  
Monitoring Program ◽  
Outdoor Environment ◽  
Susceptibility Data ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Broiler House

Antimicrobial susceptibility in Campylobacter jejuni collected from the environment outside four broiler houses (n = 63) and from the environment inside these broiler houses (including broiler droppings) (n = 36) from May to September 2004 was studied and compared with isolates from Norwegian broilers analyzed within the frame of the Norwegian monitoring program of antimicrobial resistance in feed, food, and animals (NORM-VET) in 2004 (n = 75). The MICs of oxytetracycline, ampicillin, erythromycin, gentamicin, enrofloxacin, and nalidixic acid were obtained by the broth microdilution method VetMIC. The present study, which to our knowledge is the first Norwegian study on the occurrence of antimicrobial resistance in Campylobacter spp. from the environment of broiler houses, revealed a very low occurrence of antimicrobial resistance in C. jejuni from the broilers and broiler house environments studied. All isolates originating from the four broiler houses studied were susceptible to all the antimicrobial agents tested, except for one isolate from the outdoor environment (courtyard soil), which was resistant to oxytetracycline (MIC, 8 mg/liter). For the isolates from broilers (NORM-VET), low prevalences of resistance to oxytetracycline (1.3%) and ampicillin (4%) were observed. No quinolone resistance was observed. The results for the broiler isolates are in agreement with the earlier findings of a very low prevalence of resistance in Campylobacter from broilers in Norway, which reflects the low usage of antimicrobials in Norwegian broiler production. Furthermore, the present data are in accordance with antimicrobial susceptibility data for C. jejuni from domestically acquired human cases.

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