Use of a Rapid Microbial ATP Bioluminescence Assay to Detect Contamination on Beef and Pork Carcasses†

1995 ◽  
Vol 58 (7) ◽  
pp. 770-775 ◽  
Author(s):  
GREGORY R. SIRAGUSA ◽  
CATHERINE N. CUTTER ◽  
WARREN J. DORSA ◽  
MOHAMMAD KOOHMARAIE
Keyword(s):  
Plate Count ◽  
Assay Sensitivity ◽  
Standard Plate Count ◽  
Critical Control Points ◽  
Processing Plants ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Commercial Processing ◽  
Atp Bioluminescence Assay

A new microbial ATP bioluminescence assay was shown to be an accurate and rapid method to determine the levels of generic bacterial contamination on beef (n = 400 and pork (n = 320) carcasses sampled in commercial processing plants. Based on in vitro fecal dilution studies, the rapid microbial ATP (R-mATP) assay is as accurate as the standard plate count method for estimating bacteria in bovine or porcine fecal samples. The correlations (r) between the R-mATP assay and the standard aerobic plate count for beef and pork carcasses sampled in commercial processing were 0.91 and 0.93, respectively. A segmented-model statistical approach to determine the lower limits of assay sensitivity was developed. By using this model to analyze the in-plant data, the R-mATP test responded in a linear fashion to levels of microbial contamination of > log10 2.0 aerobic CFU/cm2 on beef carcasses and of > log10 3.2 aerobic CFU/cm2 for pork carcasses. The R-mATP assay requires approximately 5 min to complete, including sampling. Given the rapidity and accuracy of the assay, processors interested in monitoring critical control points in the slaughter process could potentially use the R-mATP assay to monitor microbiological prevention and intervention procedures for minimizing carcass contamination.

Comparison of a Rapid ATP Bioluminescence Assay and Standard Plate Count Methods for Assessing Microbial Contamination of Consumers' Refrigerators

2006 ◽  
Vol 69 (10) ◽  
pp. 2534-2538 ◽  
Author(s):  
FUR-CHI CHEN ◽  
SANDRIA L. GODWIN
Keyword(s):  
Microbial Contamination ◽  
Field Studies ◽  
Plate Count ◽  
Culture Methods ◽  
Psychrotrophic Bacteria ◽  
Standard Plate Count ◽  
Plate Count Agar ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

The feasibility of using an ATP bioluminescence assay for assessing microbial contamination of home refrigerators was evaluated and compared with the standard culture methods. Samples of refrigerator surfaces were collected from 123 households by swabbing an area of 100 cm2 on three locations in the refrigerator with premoisturized sterile swabs. Microbial contaminations were determined by aerobic plate count (APC; incubated at 35°C for 48 h) and psychrotrophic plate count (PPC; incubated at 7°C for 10 days) on plate count agar. The results were compared to the readings from the microbial ATP (mATP) bioluminescence assay. The correlation coefficient (r) between mATP and PPC (r = 0.851) was slightly higher than that between mATP and APC (r = 0.823). Our results indicated a potential discrepancy in the population of mesophilic and psychrotrophic bacteria in the refrigerator samples. Nevertheless, mATP appeared to be a reliable indication of the average of APC and PPC (r = 0.895). The mATP bioluminescence assay would provide a rapid and convenient test for researchers in field studies to assess microbial contamination in refrigerators.

Adenosine Triphosphate Bioluminescence for Hygiene Monitoring in Health Care Institutions

1994 ◽  
Vol 57 (6) ◽  
pp. 509-513 ◽  
Author(s):  
KLAUS SEEGER ◽  
MANSEL W. GRIFFITHS
Keyword(s):  
Health Care ◽  
Adenosine Triphosphate ◽  
Meat Product ◽  
Plate Count ◽  
Standard Plate Count ◽  
Atp Assay ◽  
Health Care Institutions ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

An investigation was conducted to assess the practical use of an adenosine triphosphate (ATP) bioluminescence assay to evaluate the effectiveness of cleaning and sanitizing meat slicers in eight health care institutions. The ATP bioluminescence assay was compared to conventional swabbing techniques using standard plate count to enumerate microbial load. Assays were performed on meat slicers before use, after slicing a meat product and after sanitizing. There was a general overall agreement in results obtained by both methods but the ATP assay gave a better indication of the cleanliness of the meat slicer as it was able to detect the presence of meat residues left on the blade after improper sanitation. Results were available within 5 min using the ATP bioluminescence method, thus providing an opportunity for immediate remedial action.

scholarly journals Rapid Antibiotic Combination Testing for Carbapenem-Resistant Gram-Negative Bacteria within Six Hours Using ATP Bioluminescence

2018 ◽  
Vol 62 (9) ◽  
Author(s):  
Yiying Cai ◽  
Celene L. Seah ◽  
Hui Leck ◽  
Tze-Peng Lim ◽  
Jocelyn Q. Teo ◽  
...  
Keyword(s):  
Predictive Accuracy ◽  
Roc Curves ◽  
Gram Negative Bacteria ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay ◽  
Selection Of

ABSTRACTTo guide the timely selection of antibiotic combinations against carbapenem-resistant Gram-negative bacteria (CR-GNB), anin vitrotest with a short turnaround time is essential. We developed anin vitroATP bioluminescence assay to determine effective antibiotic combinations against CR-GNB within 6 h. We tested 42 clinical CR-GNB strains (14Acinetobacter baumannii, 14Pseudomonas aeruginosa, and 14Klebsiella pneumoniaestrains) against 74 single antibiotics and two-antibiotic combinations. Bacteria (approximately 5 log10CFU/ml) were incubated with an antibiotic(s) at 35°C; ATP bioluminescence was measured at 6 h and 24 h; and the measurements were compared to viable counts at 24 h. Receiver operating characteristic (ROC) curves were used to determine the optimal luminescence thresholds (TRLU) for distinguishing between inhibitory and noninhibitory combinations. The areas under the 6-h and 24-h ROC curves were compared using the DeLong method. Prospective validation of the established thresholds was conducted using 18 additional CR-GNB. The predictive accuracy ofTRLUfor the 6-h ATP bioluminescence assay was 77.5% when all species were analyzed collectively. Predictive accuracies ranged from 73.7% to 82.7% when each species was analyzed individually. Upon comparison of the areas under the 6-h and 24-h ROC curves, the 6-h assay performed significantly better than the 24-h assay (P< 0.01). Predictive accuracy remained high upon prospective validation of the 6-h ATP assay (predictive accuracy, 79.8%; 95% confidence interval [CI], 77.6 to 81.9%), confirming the external validity of the assay. Our findings indicate that our 6-h ATP bioluminescence assay can provide guidance for prospective selection of antibiotic combinations against CR-GNB in a timely manner and may be useful in the management of CR-GNB infections.

Study on the Detection of Total Colony in Medical and Health Environment Based on ATP Bioluminescence

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
Zhe Li
Keyword(s):  
Culture Method ◽  
Pass Rate ◽  
Plate Count ◽  
Count Method ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Plate Count Method ◽  
The Difference ◽  
Atp Bioluminescence Assay ◽  
Bioluminescence Method

In this paper, the application of ATP fluorescence in the detection of colonies in the health environment of hospitals was studied. Firstly, the principle of ATP bioluminescence method was described. Then, ATP bioluminescence and plate count method were used to test the density of the surface of the objects in selected area, taking the time points 2 hours after disinfection as the time nodes. The results showed that the difference between the qualified rate of ATP bioluminescence assay and the plate count method was statistically significant {P<0.01}. Therefore, ATP bioluminescence method was highly correlated with bacterial culture method. The correlation coefficient of pass rate of the two methods was 0.782, which indicated that there was a positive correlation between the two test results. Besides, the detection results showed that ATP bioluminescence method had higher sensitivity than plate counting method. Therefore, ATP bioluminescence method was more suitable for the rapid detection of the colony of hospital health environment, and helps the hospital to better manage its environmental hygiene conditions. 

scholarly journals Oleate prevents palmitate-induced mitochondrial dysfunction in chondrocytes

2020 ◽  
Author(s):  
Maria Vaquez-Mosquera ◽  
Mercedes Fernandez-Moreno ◽  
Estefania Cortes-Pereira ◽  
Sara Relaño ◽  
Andrea Dalmao-Fernandez ◽  
...  
Keyword(s):  
Mitochondrial Dysfunction ◽  
Lipid Droplets ◽  
Coupling Efficiency ◽  
Human Chondrocytes ◽  
Metabolic Phenotype ◽  
Systemic Factors ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay

Abstract BACKGROUND : The clear association between obesity and osteoarthritis (OA) in joints not subjected to mechanical overload, together with the relationship between OA and metabolic syndrome (MS), suggests that there are systemic factors related to metabolic disorders that are involved in the metabolic phenotype of OA. The aim of this work is to study the effects of palmitate (PA) and oleate (OL), as the most abundant fatty acids (FA) present in the diet and serum, on cellular metabolism in an " in vitro " model of human chondrocytes. METHODS :.The Seahorse XF96 Analyzer was used tomeasure the mitochondrial, glycolytic function and the contribution of the mitochondrial oxidative phosphorilation system (OXPHOS) and glycolysis to the production of ATP, in the T/C-28a2 chondrocyte treated with to PA, OL and palmitate/oleate (PA/OL) ratio 1:2. Subsequently ATP bioluminescence assay kit was used for ATP quantification. To detect the presence of lipid droplets, two types of stains were performed and the amount of Triglycerides was quantified spectrophotometrically. RESULTS : PA, but not OL, produces mitochondrial dysfunction observed with a lower rate of OCR intended for the synthesis of ATP, coupling efficiency, maximal respiration and spare respiratory capacity. Glycolytic function showed lower rates for both glycolytic capacity and glycolytic reserve when cells were incubated withFA in relation to basal condition (BC). The production rate of ATP from OXPHOS showed lower values in chondrocytes incubated with any of the FAs. The evaluation of possible formation of Lipid droplets (LD) showed a significant increase of these structures in FA conditions, being significantly higher when the cells were incubated with OL. CONCLUSIONS : PA and OL show antagonistic effects in human chondrocytes; while increased levels of PA induce mitochondrial dysfunction and hinder the response of chondrocytes through the glycolytic pathway, OL shows a cytoprotective effect through which promotes the formation of triglycerides-rich LD, as well as the incorporation of PA.

Rapid Assessment of the Microbiological Quality of Poultry Carcasses Using ATP Bioluminescence

1995 ◽  
Vol 58 (5) ◽  
pp. 551-554 ◽  
Author(s):  
DERRICK A. BAUTISTA ◽  
JEAN PIERRE VAILLANCOURT ◽  
ROBERT A. CLARKE ◽  
SHANE RENWICK ◽  
MANSEL W. GRIFFITHS
Keyword(s):  
Rapid Assessment ◽  
Microbiological Quality ◽  
Meat Industry ◽  
Control Points ◽  
Critical Control Points ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Plate Counts ◽  
Atp Bioluminescence Assay

The meat industry is in need of faster and more reliable methods to determine microbial loads in food products. A rapid method (&lt;15 min) has been developed to assess the microbiological quality of chicken carcasses using the adenosine triphosphate (ATP) bioluminescence assay. The results indicate that, following modifications, the ATP bioluminescence test produced an acceptable correlation with plate counts (r = 0.85, p &lt; 0.001) and demonstrated good repeatability between replicates. It is envisaged that the modified ATP bioluminescence assay would best be used as a platform rejection test. Using threshold levels determined from the regression equation, the ATP bioluminescence assays gave about 90% agreement with plate counts for carcass rinses with counts above 5 × 104 CFU/ml. These findings suggest that the modified ATP bioluminescence assay could be used for monitoring critical control points (CCPs) in programs based on hazard analysis of critical control points (HACCP).

Microbial ATP Bioluminescence as a Means to Detect Contamination on Artificially Contaminated Beef Carcass Tissue†

1995 ◽  
Vol 58 (7) ◽  
pp. 764-769 ◽  
Author(s):  
GREGORY R. SIRAGUSA ◽  
CATHERINE N. CUTTER
Keyword(s):  
Regression Line ◽  
Correlation Coefficients ◽  
Viable Count ◽  
Processing Plant ◽  
Critical Control Points ◽  
Assay Procedure ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay ◽  
Bovine Feces

The use of microbial ATP bioluminescence was evaluated as a means to rapidly detect gross microbial contamination from feces on bovine-carcass surface tissue (BCT). Microbial ATP was selectively distinguished from nonmicrobial ATP by the assay procedure used. Regression analyses of microbial ATP and viable count scatterplots showed lean and adipose BCT artificially contaminated with bovine feces had the same regression line parameters (P &lt; 0.05), and therefore, the microbial ATP responses were similar for both tissue types. Correlation coefficients (r) of these regression lines were &gt;0.90 for both tissue types. Results indicated that swab samples can be held at 5°C for up to 6 h without compromising microbial ATP bioluminescence assay results. The microbial ATP bioluminescence assay shows potential for use as a means to rapidly detect fecal contamination on red meat carcasses and to gauge decontamination effectiveness and hence could monitor critical control points in a processing-plant HACCP plan.

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