A Nucleic Acid Sequence–Based Amplification Method To Detect Salmonella enterica Serotype Enteritidis Strain PT4 in Liquid Whole Egg

2002 ◽  
Vol 65 (7) ◽  
pp. 1177-1178 ◽  
Author(s):  
N. COOK ◽  
J. ELLISON ◽  
A. S. KURDZIEL ◽  
S. SIMPKINS ◽  
J. P. HAYS
Keyword(s):  
Nucleic Acid ◽  
Salmonella Enterica ◽  
Nucleic Acid Sequence ◽  
Amplification Method ◽  
Peptone Water ◽  
Liquid Whole Egg ◽  
Whole Egg ◽  
Salmonella Enterica Serotype Enteritidis

Nucleic acid sequence–based amplification (NASBA) was applied to the detection of Salmonella enterica cells in liquid whole egg. Samples (25 g) of liquid whole egg inoculated with Salmonella cells were enriched for 16 h in buffered peptone water, and the NASBA procedure was effective in detecting the presence of Salmonella in samples inoculated with 10 to 100 CFU prior to enrichment.

Inactivation of Salmonella enterica Serovar Senftenberg 775W in Liquid Whole Egg by Ultrahigh Pressure Homogenization

2008 ◽  
Vol 71 (11) ◽  
pp. 2283-2288 ◽  
Author(s):  
R. M. VELÁZQUEZ-ESTRADA ◽  
M. M. HERNÁNDEZ-HERRERO ◽  
T. LÓPEZ-PEDEMONTE ◽  
B. GUAMIS-LÓPEZ ◽  
A. X. ROIG-SAGUÉS
Keyword(s):  
Salmonella Enterica ◽  
Storage Period ◽  
Ultrahigh Pressure ◽  
Inlet Temperature ◽  
Initial Load ◽  
Yeast Extract Medium ◽  
Liquid Whole Egg ◽  
Entire Storage Period ◽  
Thermal Pasteurization ◽  
Whole Egg

Two batches of samples of liquid whole egg were inoculated with a load of approximately 3 and 7 log CFU/ml, respectively, of Salmonella enterica serovar Senftenberg 775W and submitted to different ultrahigh pressure homogenization (UHPH) treatments at 150, 200, and 250 MPa. The inlet temperature of the samples was 6°C. Counts of viable and injured Salmonella cells were obtained 2 h after the UHPH treatments and after 5, 10, 15, and 20 days of storage at 4°C. The level of pressure applied influenced the lethality attained significantly (P < 0.05). In the samples with an initial load of approximately 7 log CFU/ml, the highest lethality value of 3.2 log CFU/ml was obtained at 250 MPa, and it is similar to those values reported in other surveys for thermal pasteurization with this same Salmonella strain. When the initial load was approximately 3 log CFU/ml, total inactivation was apparently obtained after the 250-MPa treatment (2.7 log CFU/ml). After 10 days of storage at 4°C, Salmonella counts decreased in UHPH-treated samples, and colonies were not observed in tryptone soy agar and yeast extract medium. Nevertheless, presence of viable Salmonella cells was detected with the VIDAS Salmonella immunoassay method during the entire storage period. These results encourage further investigation of UHPH processing of liquid whole egg, assaying the possibility of using higher pressures and fluid inlet temperatures.

Recovery of Salmonella from Shell Eggs

1991 ◽  
Vol 74 (5) ◽  
pp. 821-826
Author(s):  
Patricia Stephenson ◽  
Felicia B Satchell ◽  
Geraldine Allen ◽  
Wallace H Andrews
Keyword(s):  
Brain Heart Infusion ◽  
Egg Yolk ◽  
Most Probable Number ◽  
Probable Number ◽  
Selective Enrichment ◽  
Shell Eggs ◽  
Peptone Water ◽  
Egg Albumen ◽  
Liquid Whole Egg ◽  
Whole Egg

Abstract A preenrlchment procedure and a direct selective enrichment procedure were compared for recovery of Salmonella artificially inoculated into liquid whole egg, egg yolk, and egg albumen. For liquid whole egg and egg yolk, the 2 procedures were comparable. With egg albumen, however, preenrlchment In lactose broth gave significantly higher recoveries than did direct selective enrichment in either selenite cystine or tetrathionate broths. The lactose preenrlchment procedure was used to determine the survival of S. enterltldla in egg yolk and egg albumen over a period of 7 days. As shown by most probable number determinations, counts of S. enterltldla Inoculated Into egg albumen decreased by 3 log units, whereas those in egg yolk did not change significantly. It is recommended, therefore, that only the egg yolk be examined for this pathogen. In a comparison of 5 different preenrlchment media (lactose broth, brain heart Infusion broth, trypticase soy broth, buffered peptone water, and nutrient broth), lactose broth was somewhat less productive than the other 4 media for the recovery of Salmonella from egg yolks. Trypticase soy broth gave the highest recovery.

Gold-Aptamer-Nanoconstructs Engineered to Detect Conserved Enteroviral Nucleic Acid Sequences

2019 ◽  
Author(s):  
Veeren Chauhan ◽  
Mohamed M Elsutohy ◽  
C Patrick McClure ◽  
Will Irving ◽  
Neil Roddis ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
In Silico ◽  
Point Of Care ◽  
Lateral Flow ◽  
Nucleic Acid Sequence ◽  
In Silico Screening ◽  
Lateral Flow Assays ◽  
Life Threatening ◽  
Software And Hardware ◽  
Nucleic Acid Sequences

<p>Enteroviruses are a ubiquitous mammalian pathogen that can produce mild to life-threatening disease. Bearing this in mind, we have developed a rapid, accurate and economical point-of-care biosensor that can detect a nucleic acid sequences conserved amongst 96% of all known enteroviruses. The biosensor harnesses the physicochemical properties of gold nanoparticles and aptamers to provide colourimetric, spectroscopic and lateral flow-based identification of an exclusive enteroviral RNA sequence (23 bases), which was identified through in silico screening. Aptamers were designed to demonstrate specific complementarity towards the target enteroviral RNA to produce aggregated gold-aptamer nanoconstructs. Conserved target enteroviral nucleic acid sequence (≥ 1x10<sup>-7</sup> M, ≥1.4×10<sup>-14</sup> g/mL), initiates gold-aptamer-nanoconstructs disaggregation and a signal transduction mechanism, producing a colourimetric and spectroscopic blueshift (544 nm (purple) > 524 nm (red)). Furthermore, lateral-flow-assays that utilise gold-aptamer-nanoconstructs were unaffected by contaminating human genomic DNA, demonstrated rapid detection of conserved target enteroviral nucleic acid sequence (< 60 s) and could be interpreted with a bespoke software and hardware electronic interface. We anticipate our methodology will translate in-silico screening of nucleic acid databases to a tangible enteroviral desktop detector, which could be readily translated to related organisms. This will pave-the-way forward in the clinical evaluation of disease and complement existing strategies at overcoming antimicrobial resistance.</p>

Survival of Salmonella senftenberg 775W to current liquid whole egg pasteurization treatments

2003 ◽  
Vol 20 (5) ◽  
pp. 593-600 ◽  
Author(s):  
Pilar Mañas ◽  
Rafael Pagán ◽  
Ignacio Alvarez ◽  
Santiago Condón Usón
Keyword(s):  
Liquid Whole Egg ◽  
Salmonella Senftenberg ◽  
Whole Egg
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