Antimicrobial Drug Resistance of Salmonella and Escherichia coli Isolates from Cattle Feces, Hides, and Carcasses

2007 ◽  
Vol 70 (3) ◽  
pp. 551-556 ◽  
Author(s):  
W. M. FLUCKEY ◽  
G. H. LONERAGAN ◽  
R. WARNER ◽  
M. M. BRASHEARS
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Antibiotic Susceptibility ◽  
Drug Susceptibility ◽  
Antimicrobial Drug ◽  
Cross Contamination ◽  
Bacterial Isolation ◽  
Antimicrobial Drugs ◽  
Antimicrobial Drug Resistance ◽  
E Coli

To determine patterns of cross-contamination and antibiotic susceptibility of microorganisms commonly associated with cattle, 60 cattle shipped to a commercial abattoir (20 in each of three separate trial periods) were followed through processing. Samples for bacterial isolation were collected from the feces and hides immediately before shipping, from the hides at the abattoir after exsanguination, and from the carcasses before evisceration and in the cooler. Samples were cultured for Salmonella and non–type-specific Escherichia coli. Salmonella was identified in 33.9% (n = 20) of the fecal samples and on 37.3% (n = 22) of the hides before shipment. At the abattoir, the proportion of hides from which Salmonella was isolated increased (P < 0.001) to 84.2% (48 hides). Nonspecific E. coli and Salmonella were recovered from 40.4 and 8.3% of preevisceration carcass samples, respectively. No Salmonella or nonspecific E. coli were recovered from hotbox carcass samples. Isolates were tested for antimicrobial drug susceptibility. For nonspecific E. coli, 80.3% (n = 270) of the isolates were resistant to at least one antimicrobial drug. For Salmonella, 97% (n = 101) of the isolates were resistant to at least one antimicrobial drug; however, only 4.0% were resistant to two or more. The most common resistance was to sulfamethoxazole. These results indicate that the presence of microorganisms resistant to antimicrobial drugs is common in cattle and beef. Further studies are needed to identify the sources and causes of this drug resistance.

scholarly journals Antimicrobial Drug Resistance in Escherichia coli from Humans, and Identification of Carbapenemase-Producing E. coli in the City of Zabol, Iran

2018 ◽  
Vol 5 (3) ◽  
Author(s):  
Khadije Rezaie Keikhaie ◽  
Fatemeh Moshtaghi ◽  
Maryam Sheykhzade Asadi ◽  
Samira Seyed Nejad ◽  
Gholamreza Bagheri
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Antimicrobial Drug ◽  
Antimicrobial Drug Resistance ◽  
E Coli ◽  
The City

scholarly journals Antimicrobial Drug Resistance of Escherichia coli Isolated from Piglets in North East India

2020 ◽  
pp. 195-205
Author(s):  
Hosterson Kylla ◽  
Tapan Kumar Dutta ◽  
Parimal Roychoudhury ◽  
Prasant Kumar Subudhi ◽  
T. C. Tolenkhomba ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Farming System ◽  
Antimicrobial Drug ◽  
Weaned Piglets ◽  
Fecal Samples ◽  
Antimicrobial Drug Resistance ◽  
E Coli ◽  
North East ◽  
North Eastern

Aim: The research is aimed to study the antimicrobial drug resistance (AMR) of Escherichia coli from fecal samples of pre-weaned piglets in North Eastern states (NE) of India. Materials and Methods: A total of 457 fresh fecal samples were collected from pre-weaned piglets of organized (n=225) and unorganized (n=232) farms of 4 North Eastern states of India, namely, Manipur, Meghalaya, Mizoram and Nagaland state. Samples were collected from diarrheic (n=339) and non-diarrheic (n=118) piglets in different seasons during the study period. The samples were processed for isolation of E. coli and detection of their putative virulence genes by polymerase chain reaction (PCR). Results: A total of 1286 E. coli were isolated. Forty-two isolates (3.26%) were found to be atypical enteropathogenic E. coli (aEPEC) and thirty isolates (2.33%) belongs to shigatoxigenic E. coli (STEC) in which 28 isolates were positive for stx2 gene and two isolates possessed hlyA gene. All the 1286 isolates showed wide variation (0.15-78.69%) in resistance pattern against the 15 antimicrobial agents; of which there is higher resistance against cefalexin (78.69%), amoxycillin (77.13%), ampicillin (72.31%) and enrofloxacin (60.73%). The isolates showed low resistance to imipenem (0.15%), ceftriaxone (8.32%), ciprofloxacin (8.39%) and streptomycin (8.94%). Higher prevalence of AMR to numerous antimicrobials in this study was observed in isolates of organized farm compared to unorganized farming system. Conclusion: The present study exhibited variation in AMR in different NE states of India as well as in different farming system which indicate that drug consumption and resistance are closely related.

scholarly journals Antimicrobial drug resistance of Escherichia coli isolated from poultry abattoir workers at risk and broilers on antimicrobials

2008 ◽  
Vol 79 (4) ◽  
Author(s):  
J.W. Oguttu ◽  
C.M. Veary ◽  
J.A. Picard
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Inhibitory Concentration ◽  
Enteric Bacteria ◽  
Antimicrobial Drug ◽  
Control Group ◽  
Antimicrobial Drug Resistance ◽  
E Coli ◽  
The People ◽  
Abattoir Workers

Antimicrobial usage in food animals increases the prevalence of antimicrobial drug resistance among their enteric bacteria. It has been suggested that this resistance can in turn be transferred to people working with such animals, e.g. abattoir workers. Antimicrobial drug resistance was investigated for Escherichia coli from broilers raised on feed supplemented with antimicrobials, and the people who carry out evisceration, washing and packing of intestines in a high-throughput poultry abattoir in Gauteng, South Africa. Broiler carcasses were sampled from 6 farms, on each of which broilers are produced in a separate 'grow-out cycle'. Per farm, 100 caeca were randomly collected 5 minutes after slaughter and the contents of each were selectively cultured for E. coli. The minimum inhibitory concentration (MIC) of each isolate was determined for the following antimicrobials : doxycycline, trimethoprim, sulphamethoxazole, ampicillin, enrofloxacin, fosfomycin, ceftriaxone and nalidixic acid. The same was determined for the faeces of 29 abattoir workers and 28 persons used as controls. The majority of isolates from broilers were resistant, especially to antimicrobials that were used on the farms in the study. Overall median MICs and the number of resistant isolates from abattoir workers (packers plus eviscerators) tended to be higher than for the control group. However, no statistically significant differences were observed when the median MICs of antimicrobials used regularly in poultry and percentage resistance were compared, nor could an association between resistance among the enteric E. coli from packers and those from broilers be demonstrated.

Genotypic and phenotypic situation of antimicrobial drug resistance of Escherichia coli in water and manure between biogas and non-biogas swine farms in central Thailand

2021 ◽  
Vol 279 ◽  
pp. 111659
Author(s):  
Atchara Dawangpa ◽  
Preeda Lertwatcharasarakul ◽  
Pongrama Ramasoota ◽  
Alongkot Boonsoongnern ◽  
Nattavut Ratanavanichrojn ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Antimicrobial Drug ◽  
Antimicrobial Drug Resistance ◽  
Swine Farms ◽  
Central Thailand

Antimicrobial Drug Resistance in Escherichia coli Including an O157:H7 Isolate from Feces of Healthy Goats in Grenada

2015 ◽  
Vol 7 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Victor Amadi ◽  
Estefania Avendano ◽  
Ozioma Onyegbule ◽  
Zachary Pearl ◽  
Stratton Graeme ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Antimicrobial Drug ◽  
Antimicrobial Drug Resistance

Surveillance of the Antimicrobial Resistance Rates of Helicobacter pylori Ten Years Later in the Western Central Region, Colombia

2019 ◽  
Vol 38 (3) ◽  
pp. 196-203
Author(s):  
Ingrid Johanna Bedoya-Gómez ◽  
Adalucy Alvarez-Aldana ◽  
José Ignacio Moncayo-Ortiz ◽  
Yina Marcela Guaca-González ◽  
Jorge Javier Santacruz-Ibarra ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Helicobacter Pylori ◽  
Antimicrobial Drug ◽  
Test Method ◽  
Clinical Isolates ◽  
Moderate Increase ◽  
Antimicrobial Drugs ◽  
Antimicrobial Drug Resistance ◽  
H Pylori ◽  
Resistance Rates

Background: Helicobacter pylori is a bacterium associated with gastroduodenal disease and gastric cancer. Empirical therapy in the treatment of H. pylori infection increases the risk of apparition of antimicrobial drug resistance. In a previous report, in H. pylori clinical isolates, resistance rates to commonly used antimicrobial drugs were as follows: metronidazole 82%, clarithromycin 3.8%, and amoxicillin 1.9%. The aim was to establish the variation of resistance rates and the detection of H. pylori genetic mutations isolated from dyspeptic patients. Methods: Antimicrobial susceptibility profiles were performed by the E-test method for metronidazole, clarithromycin, amoxicillin, and tetracycline in 61 clinical isolates. Sequencing was performed to detect mutations associated with resistance to clarithromycin. Results: According to our results, resistance rates found in the 61 isolates were 78.60% for metronidazole and 8.20% for clarithromycin. None of the studied isolates had resistance to tetracycline and amoxicillin. Secondary resistance rates displayed an increase when compared to primary rates for metronidazole (87.50 vs. 77.35%) and for clarithromycin (25.66 vs. 5.66%). Of 5 isolates resistant to clarithromycin, 3 had the A2143G mutation. By comparing the results in this work with previous reports, antimicrobial drug resistance rates did not show major modifications for metronidazole, amoxicillin, and tetracycline during the last 10 years. For clarithromycin, the resistance rate showed a moderate increase; nevertheless, it remains low (<15%) and this change was not statistically significant. Conclusion: Together, all findings in this work indicate that these antimicrobial drugs can still be used as first line of defense on infected patients living in this region of the country.

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