Forensic DNA technology

2022 ◽  
pp. 94-110
Author(s):  
Christopher Lawless
Keyword(s):  
2020 ◽  
pp. 363-372

The first applications of DNA technology in criminal cases took place in the United States and United Kingdom more than 30 years ago. What have we learned over the past three decades from the use of forensic DNA analysis in criminal and human rights investigations and humanitarian disasters? And what challenges, opportunities, and potential pitfalls lie ahead?...


1991 ◽  
Vol 29 (01) ◽  
pp. 29-0295-29-0295
Keyword(s):  

Author(s):  
Scott Turow

Forensic DNA evidence has helped convict the guilty, exonerate the wrongfully convicted, identify victims of genocide, and reunite families torn apart by war and repressive regimes. Yet many of the scientific, legal, and ethical concepts that underpin forensic DNA evidence remain unclear to the general public; judges; prosecutors; defense attorneys; and students of law, forensic sciences, ethics, and genetics. This book examines the history and development of DNA forensics; its applications in the courtroom and humanitarian settings; and the relevant scientific, legal, and psychosocial issues. It describes the DNA technology used to compare the genetic profile of a crime scene sample to that of a suspect, as well as the statistical interpretation of a match. It also reviews how databases can be searched to identify suspects and how DNA evidence can be used to exonerate the wrongfully convicted. Recent developments in DNA technology are reviewed, as are strategies for analyzing samples with multiple contributors. The book recounts how the Grandmothers of the Plaza de Mayo searched for children kidnapped during military rule in Argentina, as well as more recent efforts to locate missing children in El Salvador. Other chapters examine the role that DNA forensics played in the identification of victims of genocide in Bosnia and of terrorism in the post-9/11 era. Social anthropologists, legal scholars, and scientists explore current applications of DNA analysis in human trafficking and mass catastrophes; border policies affecting immigration; and the ethical issues associated with privacy, informed consent, and the potential misuse of genetic data.


Author(s):  
M.L. Tate

Traceability requirements for market access, food safety and disease control are a key issue in the meat industry. Traceability also offers new opportunities for market-lead product improvement and genetic selection. DNA technology has recently provided a solution to the problem of tracing meat cuts from overseas markets back to the New Zealand processor's carcass record and the farm of origin. The DNA system involves storing a sample of every carcass processed, and using forensic DNA matching to identify the ensuing meat cuts. While the system has solved one aspect of traceability, significant challenges remain to provide cost effective meat traceability for market assurance and product improvement. Keywords: animal welfare, consumers, DNA, market access, traceability


2009 ◽  
Vol 40 (11) ◽  
pp. 24-25
Author(s):  
DOUG BRUNK
Keyword(s):  

2020 ◽  
Vol 10 (1) ◽  
pp. 56-61
Author(s):  
Vishal Somnay ◽  
Thomas Duong ◽  
Ray-Young Tsao ◽  
Joseph A. Prahlow

Forensic DNA testing can play a critical role in homicide investigations. Selecting the appropriate evidence on which to perform DNA testing requires foresight and reasoning based on experience and science. Although successful DNA testing can occur using many substrates, including blood, hair, and sweat/epithelial cells, positive results can also result from testing various unorthodox samples. The authors report on a triple-murder investigation where DNA testing of dog feces at the crime scene matched DNA testing of feces found on the shoe of a suspect resulting in successful prosecution of the case.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hashom Mohd Hakim ◽  
Hussein Omar Khan ◽  
Siti Afifah Ismail ◽  
Nurul Hazirah Mat Lazim ◽  
Japareng Lalung ◽  
...  

Abstract Background DNA profiling has proven to be a valuable technique for identification of individuals in crime. Currently, the technique targets several short tandem repeat (STR) regions in human genome. However, increasing number of samples submitted for STR analysis may lead to delays due to the limited number of experienced analysts who might be available at any given moment and the time taken to complete lengthy DNA profiling procedures. This study was conducted to test the specificity, repeatability, reproducibility and robustness of Investigator® 24plex GO! kit for genotyping of reference samples submitted to the Royal Malaysian Police Forensic DNA Laboratory for DNA database. Material and methods In this study, Investigator® 24plex GO! kit was used to directly amplify STR loci from buccal swab cell of reference samples that had previously been STR typed using GlobalFiler™ Express kit. Capillary electrophoresis was carried out on a 3500xL Genetic Analyser using POP-4® Polymer. Amplified products were assigned to particular STR alleles using the GeneMapper ID-X version 1.4 software. Results Our study shows that STR profiles generated using Investigator® 24plex GO! gave concordance results with those previously obtained using the GlobalFiler™ Express kit. In addition, quality sensors included in the kit are of particular importance for determining the effectiveness of the PCR reaction and help to indicate the nature and quantity of DNA template for PCR amplification. Conclusion The Investigator® 24plex GO! kit is reliable for STR typing of reference samples.


Genetics ◽  
2000 ◽  
Vol 155 (4) ◽  
pp. 1973-1980
Author(s):  
Jinko Graham ◽  
James Curran ◽  
B S Weir

Abstract Modern forensic DNA profiles are constructed using microsatellites, short tandem repeats of 2–5 bases. In the absence of genetic data on a crime-specific subpopulation, one tool for evaluating profile evidence is the match probability. The match probability is the conditional probability that a random person would have the profile of interest given that the suspect has it and that these people are different members of the same subpopulation. One issue in evaluating the match probability is population differentiation, which can induce coancestry among subpopulation members. Forensic assessments that ignore coancestry typically overstate the strength of evidence against the suspect. Theory has been developed to account for coancestry; assumptions include a steady-state population and a mutation model in which the allelic state after a mutation event is independent of the prior state. Under these assumptions, the joint allelic probabilities within a subpopulation may be approximated by the moments of a Dirichlet distribution. We investigate the adequacy of this approximation for profiled loci that mutate according to a generalized stepwise model. Simulations suggest that the Dirichlet theory can still overstate the evidence against a suspect with a common microsatellite genotype. However, Dirichlet-based estimators were less biased than the product-rule estimator, which ignores coancestry.


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