scholarly journals Screening Wild Yeast Strains for Alcohol Fermentation from Various Fruits

Mycobiology ◽  
2011 ◽  
Vol 39 (1) ◽  
pp. 33 ◽  
Author(s):  
Yeon-Ju Lee ◽  
Yu-Ri Choi ◽  
So-Young Lee ◽  
Jong-Tae Park ◽  
Jae-Hoon Shim ◽  
...  
Author(s):  
Hiroaki Negoro ◽  
Atsushi Kotaka ◽  
Hiroki Ishida

ABSTRACT Saccharomyces cerevisiae produces organic acids including malate during alcohol fermentation. Since malate contributes to the pleasant flavor of sake, high-malate-producing yeast strain No. 28 and No. 77 have been developed by the Brewing Society of Japan. In this study, the genes responsible for the high malate phenotype in these strains were investigated. We had found previously that the deletion of components of the glucose induced degradation-deficient (GID) complex led to high malate production in yeast. Upon examining GID protein-coding genes in yeast strain No. 28 and No. 77, a nonsense homozygous mutation of GID4 in strain No. 28, and of GID2 in strain No. 77, were identified as the cause of high malate production. Furthermore, complementary tests of these mutations indicated that the heterozygous nonsense mutation in GID2 was recessive. In contrast, the heterozygous nonsense mutation in GID4 was considered semi-dominant.


2018 ◽  
Author(s):  
Jennie J. Kuzdzal-Fick ◽  
Lin Chen ◽  
Gábor Balázsi

ABSTRACTMulticellular organisms appeared on Earth through several independent major evolutionary transitions. Are such transitions reversible? Addressing this fundamental question entails understanding the benefits and costs of multicellularity versus unicellularity. For example, some wild yeast strains form multicellular clumps, which might be beneficial in stressful conditions, but this has been untested. Here we show that unicellular yeast evolves from clump-forming ancestors by propagating samples from suspension after larger clumps have settled. Unicellular yeast strains differed from their clumping ancestors mainly by mutations in the AMN1 (Antagonist of Mitotic exit Network) gene. Ancestral yeast clumps were more resistant to freeze/thaw, hydrogen peroxide, and ethanol stressors than their unicellular counterparts, while unicellularity was advantageous without stress. These findings inform mathematical models, jointly suggesting a trade-off between the benefits and downsides of multicellularity, causing bet-hedging by regulated phenotype switching as a survival strategy in unexpected stress.


2008 ◽  
Vol 66 (3) ◽  
pp. 184-187 ◽  
Author(s):  
Luis C. Damas-Buenrostro ◽  
Gerardo Gracia-González ◽  
Carlos E. Hernández-Luna ◽  
Luis J. Galán-Wong ◽  
Benito Pereyra-Alférez ◽  
...  
Keyword(s):  

Biomolecules ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 890
Author(s):  
Tomasz Tarko ◽  
Aleksandra Duda-Chodak ◽  
Paweł Sroka ◽  
Magdalena Januszek

The micro-oxygenation of musts may affect the quality of a finished alcoholic beverage. The aim of this study was to determine the effect of micro-oxygenation at various stages of fermentation on oenological parameters, antioxidant activity, total polyphenol content, and profile of volatile cider compounds fermented with various yeast strains. Rubin cultivar must was inoculated with wine yeast, cider yeast, distillery yeast, and wild yeast strains. Some of the inoculated samples were oxygenated immediately after yeast inoculation, and some on the second and third fermentation days. The control sample was non-oxygenated must fermented in bottles. Higher extract concentration and acidity as well as lower potency were observed in cider treated with micro-oxygenation. Must oxygenation in most cases contributed to the reduction of polyphenol content and to the antioxidant activity of ciders, especially when fermented using wild yeast. The oxygenation of musts before fermentation caused an increase in the content of esters and alcohols in ciders. However, the oxygenation of musts during fermentation reduced the concentration of these volatile components. The oxygenation of musts during fermentation produced a differentiated effect on terpenoid concentration in ciders.


2000 ◽  
Vol 23 (1) ◽  
pp. 156-164 ◽  
Author(s):  
Spiros Paramithiotis ◽  
Martin R.A. Müller ◽  
Matthias A. Ehrmann ◽  
Effie Tsakalidou ◽  
Herbert Seiler ◽  
...  
Keyword(s):  

2018 ◽  
Vol 32 (S1) ◽  
Author(s):  
Molly R. Loughrin ◽  
Stephen Nold ◽  
Rana Delshadi

2018 ◽  
Author(s):  
Allison W. McClure ◽  
Katherine C. Jacobs ◽  
Trevin R. Zyla ◽  
Daniel J. Lew

SummaryStudies of lab strains of Saccharomyces cerevisiae have uncovered signaling pathways involved in mating, including information processing strategies to optimize decisions to mate or to bud. However, lab strains are heterothallic (unable to self-mate) while wild yeast are homothallic. And while mating of lab strains is studied using cycling haploid cells, mating of wild yeast is thought to involve germinating spores. Thus, it was unclear whether lab strategies would be appropriate in the wild. Here, we have investigated the behaviors of several yeast strains derived from wild isolates. Following germination, these strains displayed large differences in their propensity to mate or to enter the cell cycle. The variable interest in sex following germination was correlated with differences in pheromone production, which were due to both cis- and trans-acting factors. Our findings suggest that yeast spores germinating in the wild may often enter the cell cycle and form microcolonies prior to engaging in mating.


2006 ◽  
Vol 12 (3) ◽  
pp. 194-198 ◽  
Author(s):  
I. PAPATHANASIOU ◽  
R. SELVAGINI ◽  
M. SERVILI ◽  
A. VAUGHAN-MARTINI ◽  
I.G. ROUSSIS

Foods ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 287 ◽  
Author(s):  
Alice Agarbati ◽  
Laura Canonico ◽  
Enrica Marini ◽  
Emanuele Zannini ◽  
Maurizio Ciani ◽  
...  

In the last decades, there has been a growing interest from consumers in their food choices. Organic, natural, less processed, functional, and pre-probiotic products were preferred. Although, Saccharomyces cerevisiae var. boulardii is the most well-characterized probiotic yeast available on the market, improvement in probiotic function using other yeast species is an attractive future direction. In the present study, un-anthropized natural environments and spontaneous processed foods were exploited for wild yeast isolation with the goal of amplifying the knowledge of probiotic aptitudes of different yeast species. For this purpose, 179 yeast species were isolated, identified as belonging to twelve different genera, and characterized for the most important probiotic features. Findings showed interesting probiotic characteristics for some yeast strains belonging to Lachancea thermotolerans, Metschnikowia ziziphicola, Saccharomyces cerevisiae, and Torulaspora delbrueckii species, although these probiotic aptitudes were strictly strain-dependent. These yeast strains could be proposed for different probiotic applications, such as a valid alternative to, or in combination with, the probiotic yeast S. cerevisiae var. boulardii.


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