scholarly journals Effect of polymorphisms in the µ-calpain and calpastatin genes on economically important traitsof beef cattle in Puerto Rico

1969 ◽  
Vol 99 (2) ◽  
pp. 87-104
Author(s):  
Jonael Bosques ◽  
Melvin Pagán-Morales ◽  
Américo Casas ◽  
Aixa Rivera ◽  
Danilo Cianzio
Keyword(s):  
Puerto Rico ◽  
Growth Traits ◽  
Somatic Growth ◽  
Biceps Femoris ◽  
Daily Weight Gain ◽  
Meat Tenderness ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Weaning Age ◽  
Dietary Treatments

The associations between single nucleotide polymorphisms (SNP) in the µ-calpain (CAPN1-316 and CANP1-4751) and calpastatin (CAST) genes and growth traits were evaluated in a population of Senepol, Charoláis and Senepol x Charolais bulls (n=99). In another study, associations were evaluated between these SNP and carcass traits of a subgroup of 42 animals submitted to three dietary treatments. The SNP CAPN1-316 was associated with daily weight gain at 205 and 240 d, and with weaning age, wherein animals with CG genotype were heavier, gained weight faster, and were younger at weaning than animals with GG genotype. The latter presented heavier Biceps femoris, Semitendinosus, and Gluteus spp. muscles, but CG animals had a superior Longissimus dorsiarea. The CAPN1-4751 gene was related to birth weight, Longissimus dorsi weight, and with muscle to bone ratio, and for these traits the TT genotype was superior to the CT. The CAPN1-316 and CAPN1-4751 C alleles were found to be correlated with differences in meat tenderness measured at 0 d postmortem. The CAST SNP was not associated with any of the traits evaluated in this experiment. These results indicate that nucleotide substitution in the CAPN1 gene can produce differences in somatic growth in young bulls selected for beef production in Puerto Rico.

scholarly journals Genetic variations in the Myostatin gene affecting growth traits in sheep

2021 ◽  
Vol 14 (2) ◽  
pp. 475-482
Author(s):  
Noha M. Osman ◽  
Heba I. Shafey ◽  
Mohamed A. Abdelhafez ◽  
Ahmed M. Sallam ◽  
Karima F. Mahrous
Keyword(s):  
Growth Traits ◽  
Direct Sequencing ◽  
Muscle Growth ◽  
Daily Weight Gain ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Breeding Programs ◽  
Myostatin Gene ◽  
Mstn Gene ◽  
First Intron

Background and Aim: Sheep productivity in developing countries is crucial, as this animal is an essential source of meat and wool. Myostatin (MSTN) plays an important role in the regulation of muscle mass through the regulation of muscle growth, differentiation, and regeneration. The present study sought to investigate genetic variation in the first intron of the MSTN gene and the association of variants with growth traits in major sheep breeds in Egypt (Barki, Ossimi, and Rahmani) and Saudi Arabia (Najdi) using polymerase chain reaction (PCR) and sequencing. Materials and Methods: Blood samples were collected, and DNA was extracted from 75 animals. A 386 bp fragment in the first intron of the MSTN gene was amplified using PCR. Polymorphic sites were detected using direct sequencing and then correlated with growth traits using a general linear model. Results: Sequence analysis of the first intron of MSTN gene identified six single-nucleotide polymorphisms (SNPs) in the studied breeds. Four mutual SNPs were determined: c.18 G>T, c.241 T>C, c.243 G>A, and c.259 G>T. In addition, two SNPs c.159 A>T and c.173 T>G were monomorphic (AA and TT, respectively) in the Ossimi, Rahmani, and Najdi breeds and polymorphic in the Barki breed. The association analysis revealed that the c.18 G>T and c.241 C>T significantly associated (p<0.05) with birth weight and average daily weight gain, respectively. Conclusion: Our results strongly support MSTN as a candidate gene for marker-assisted selection in sheep breeding programs. Furthermore, the identified variants may be considered as putative markers to improve growth traits in sheep.

scholarly journals Melanocortin-4 Receptor (MC4R) gene polymorphism and its effect on growth traits in Madura cattle

2019 ◽  
Vol 44 (1) ◽  
pp. 38
Author(s):  
P. W. Prihandini ◽  
S. Sumadi ◽  
G. Suparta ◽  
D. Maharani
Keyword(s):  
Growth Traits ◽  
Balance Control ◽  
Direct Sequencing ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Mc4r Gene ◽  
Melanocortin 4 Receptor ◽  
Pcr Rflp ◽  
Reverse Primer ◽  
Selection For

Melanocortin-4 receptor (MC4R) gene has an important role in the regulation of feed intake and energy balance control. The objective of this study was to identify the single nucleotide polymorphisms (SNPs) of MC4R gene and their association with growth traits in Madura cattle. A total of 198 calves were used in this study.Forward primer: 5’-GTCGGGCGTCTTGTTCATC-3’and reverse primer: 5’-GCTTGTGTTTAGCATCGCGT-3’ were used to amplify approximately 493 bp of MC4R gene. The results showed that two SNPs, g.1133C>G and g.1108C>T were identified by direct sequencing. The PCR-RFLP method was performed to genotype all individuals studied based on SNP g.1133C>G, and its SNP was significantly associated with shoulder height (SH) at yearling age (P<0.05). Animals with GG genotype had a higher SH (110.35±6.40cm) than those with CC (102.00±8.00 cm) and CG genotype (105.96±6.23 cm). The SNP g.1133 C>G changed amino acid from valine to leucine. In conclusion, the SNP g.1133C>G of the MC4R gene may be used as a marker-assisted selection for SH trait in Madura cattle.

Associated analysis of single nucleotide polymorphisms of IGF2 gene’s exon 8 with growth traits in Wuzhishan pig

2009 ◽  
Vol 37 (1) ◽  
pp. 497-500 ◽  
Author(s):  
Guanyu Hou ◽  
Dongjin Wang ◽  
Song Guan ◽  
Hongpu Zeng ◽  
Xianzhou Huang ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Growth Traits ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Wuzhishan Pig

scholarly journals Segregation of polymorphisms in μ-calpain and calpastatin in beef cattle in Puerto Rico

1969 ◽  
Vol 99 (2) ◽  
pp. 105-116
Author(s):  
Jonael Bosques ◽  
Melvin Pagán-Morales ◽  
Américo Casas ◽  
Aixa Rivera ◽  
Danilo Cianzio
Keyword(s):  
Puerto Rico ◽  
Single Nucleotide Polymorphisms ◽  
Beef Cattle ◽  
Puerto Rican ◽  
Nucleotide Polymorphisms ◽  
Phenotypic Data ◽  
Single Nucleotide ◽  
Equal Distribution ◽  
Allelic Frequencies

The distribution of single nucleotide polymorphisms (SNP) in two regions of Calpain (CAPN1-316 and CAPN1-4751) and a SNP in Calpastatin (CAST) was determined in beef cattle raised in Puerto Rico (n=271). Genotypic and allelic frequencies were determined for each SNP in Senepol (n=53), Charolais (n=49), Angus (n=27), Charbray (n=38), Brahman (n=16), Zebu (n=16) and crossbred bulls (n=72). For CAPN1-ZAB, the global genotypic frequencies (n=219) were 0.07/CC (n=15), 0.38/CG (n=83) and 0.55/GG (n=121) with allelic frequencies of 0.21/C and 0.74/G. The CC genotype was absent in Charbray, Charoláis, Angus, Zebu and Brahman bulls. In Charolais, Zebu, Brahman and Charbray the GG genotype was in greater proportion than CG, while the inverse was observed in the Senepol breed. In Angus and crossbred animals, the CG and GG genotype were found in equal distribution. The global genotypic frequencies for CAPN1-4751 (n=256) were 0.17/CC (n=44), 0.45/CT (n=114) and 0.38/TT (n=98). The allelic frequencies were 0.39/C and 0.61/T. Animals inheriting the CT genotype were more frequent in Charolais, Senepol, Angus and crossbred bulls, while the TT was more common in Charbray and Brahman. For CAST, the global genotypic frequencies (n=261) were 0.04/CC (n=10), 0.26/CT (n=68), and 0.70/TT (n=183), respectively. The global allelic frequencies were 0.17/C and 0.83/T. The CC genotype was not found in Charoláis, Angus and Brahman breeds. The TT animals were more frequent in all breeds, as well as in the crossbred population. The segregation of polymorphisms in CAPN1 and CAST could potentially be associated with differences in economically important traits for Puerto Rican beef cattle, but this possibility should be evaluated by genotyping a broad range of animals with detailed phenotypic data before incorporating these SNP into markerassisted selection programs.

scholarly journals Single-nucleotide polymorphisms in FABP4 gene associated with growth traits in Egyptian sheep

2020 ◽  
Vol 13 (6) ◽  
pp. 1126-1132
Author(s):  
Heba Ibrahim Shafey ◽  
Karima Fathy Mahrous ◽  
Amal Ahmed Mohamed Hassan ◽  
Hossam Eldin Rushdi ◽  
Mohamed Abd El-Aziz Mohamed Ibrahim
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Growth Traits ◽  
Average Daily Gain ◽  
Correlation Coefficients ◽  
Nucleotide Polymorphisms ◽  
Negative Effects ◽  
Single Nucleotide ◽  
Fatty Acid Binding ◽  
Sheep Breeds ◽  
Intron 1

Aim: The present study was performed to assess the association of single-nucleotide polymorphisms (SNPs) in the fatty acid-binding protein 4 (FABP4) gene with birth weight (BW), final weight (FW), and average daily gain (ADG) in three Egyptian sheep breeds. Materials and Methods: Genomic DNA was extracted from the blood samples of 50 male and female individuals representing Ossimi, Rahmani, and Barki sheep breeds. A 407 bp nucleotide (nt) segment from the first intron of FABP4 was amplified by polymerase chain reaction, sequenced, and analyzed in the different samples. Results: Sequence analysis of the determined segment (407 bp) revealed four SNPs (all transition types) at nt position 372 (CP011894.1:g.57605471) A>G, nt position 211 (CP011894.1:g.57605632) A>G, nt position 143 (CP011894.1:g.57605700) T>C, and nt position 111 (CP011894.1:g.57605732) T>C. The allelic and genotypic frequencies for the identified SNPs in the sheep breeds were calculated. At nt positions 372 and 211, two alleles were identified (A and G). Only two genotypes were present at nt position 372 (AA and AG), while three genotypes were present at nt position 211 (AA, AG, and GG). Two alleles (T and C) and three identified genotypes (TT, TC, and CC) were detected at nt positions 143 and 111. Analysis of the results revealed that AA genotype at nt position 372 is associated with higher estimates for BW, FW, and ADG when compared to all the other genotypes. Very high correlation coefficients were found between the genotypes 143-TT and 111-TT and also between 143-TC and 111-TC. The genotypes 372-AG, 211-GG, 211-AA, 143-TT, 143-CC, 111-TT, 111-TC, and 111-CC were associated with negative effects on BW, FW, and ADG. Conclusion: The detection of four SNPs in a partial sequence of the Egyptian ovine FABP4 gene intron 1 reflected that this gene harbors substantial diversity. In addition, a novel SNP at nt position 372 (CP011894.1:g.57605471) A>G was associated with higher estimates for BW, FW, and ADG.

scholarly journals Association between Single Nucleotide Polymorphisms in SIRT1 and SIRT2 Loci and Growth in Tibetan Sheep

Animals ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1362
Author(s):  
Lin-sheng Gui ◽  
Sayed Haidar Abbas Raza ◽  
Li Zhou ◽  
Matthew Garcia ◽  
Ayman Hassan Abd El-Aziz ◽  
...  
Keyword(s):  
Adult Stage ◽  
Growth Traits ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Association Analyses ◽  
Qpcr Analysis ◽  
Tibetan Sheep ◽  
Silent Information Regulator 1 ◽  
Polymerase Chain ◽  
Tissues Expression

Silent information regulator 1 and 2 (SIRT1, 2) were NAD+-dependent histone or non-histone deacetylase, which emerged as key metabolic sensors in several tissues of mammals. In the present study, the search for polymorphisms within the ovine SIRT1 and SIRT2 loci as well as association analyses between SNPs and growth-related traits were performed in Tibetan sheep. To determine the expression pattern of SIRT1 and SIRT2 genes in Tibetan sheep, the quantitative real-time polymerase chain reaction (qPCR) analysis revealed that those two genes were widely expressed in diverse tissues. Expression of SIRT1 was less in abomasum of lamb, whereas it was greater in duodenum within adult stage. In the case of SIRT2, the greatest expression was observed in reticulum (lamb) and in muscle (adult), whereas the least expression was in liver for lamb and in kidney for adult animals. The association analysis demonstrated that g.3148 C > T polymorphism of SIRT1 affected heart girth (p = 0.002). The g.8074 T > A SNP of SIRT2 had a significant correlation with body weight (p = 0.011) and body length (p = 0.008). These findings suggested that the SIRT1 and SIRT2 polymorphism was involved in growth-related traits in Tibetan sheep, which may be considered to be genetic markers for improving the growth traits of Tibetan sheep.

Association of single nucleotide polymorphisms in the insulin and growth hormone gene with growth traits of Mia Chicken

2019 ◽  
Author(s):  
Nguyen Hoang Thinh ◽  
Hoang Anh Tuan ◽  
Nguyen Thi Vinh ◽  
Bui Huu Doan ◽  
Nguyen Thi Phuong Giang ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Single Nucleotide Polymorphisms ◽  
Growth Traits ◽  
Marker Gene ◽  
Average Daily Gain ◽  
Growth Hormone Gene ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Chicken Breed ◽  
Gh Gene

This study was conducted in Mia chicken breed to evaluate the association between four single nucleotide polymorphisms (SNPs), in insulin (INS) and growth hormone (GH) genes, with growth traits. Three genotypes for the SNP A3971G of INS gene and the SNP G662A and C423T of GH gene were present in the population while only two genotypes were found in the Mia chicken breed for SNP T3737C of INS gene (TT and TC). The SNP T3737C INS gene and G662A GH gene had significant association with growth traits (P less than 0.05). A significant association of T3737C INS gene with body weight (BW) was observed at 10 to 12 weeks of age and average daily gain (ADG) at 6-8 weeks of age. The SNP G662A of the GH gene was significantly associated (P less than 0.05) with BW of Mia chicken at ages from 7 to 14 weeks and with ADG (4-6; 6-8; 8-10; 10-12 and 2-16 weeks). Chicken with the GG genotype had greater BW and ADG compared to the other genotypes. The results demonstrated that this SNP G662A GH gene may be used as a candidate marker gene for genetic improvement of growth traits in Mia chicken breed.

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