scholarly journals Single-nucleotide polymorphisms in FABP4 gene associated with growth traits in Egyptian sheep

2020 ◽  
Vol 13 (6) ◽  
pp. 1126-1132
Author(s):  
Heba Ibrahim Shafey ◽  
Karima Fathy Mahrous ◽  
Amal Ahmed Mohamed Hassan ◽  
Hossam Eldin Rushdi ◽  
Mohamed Abd El-Aziz Mohamed Ibrahim
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Growth Traits ◽  
Average Daily Gain ◽  
Correlation Coefficients ◽  
Nucleotide Polymorphisms ◽  
Negative Effects ◽  
Single Nucleotide ◽  
Fatty Acid Binding ◽  
Sheep Breeds ◽  
Intron 1

Aim: The present study was performed to assess the association of single-nucleotide polymorphisms (SNPs) in the fatty acid-binding protein 4 (FABP4) gene with birth weight (BW), final weight (FW), and average daily gain (ADG) in three Egyptian sheep breeds. Materials and Methods: Genomic DNA was extracted from the blood samples of 50 male and female individuals representing Ossimi, Rahmani, and Barki sheep breeds. A 407 bp nucleotide (nt) segment from the first intron of FABP4 was amplified by polymerase chain reaction, sequenced, and analyzed in the different samples. Results: Sequence analysis of the determined segment (407 bp) revealed four SNPs (all transition types) at nt position 372 (CP011894.1:g.57605471) A>G, nt position 211 (CP011894.1:g.57605632) A>G, nt position 143 (CP011894.1:g.57605700) T>C, and nt position 111 (CP011894.1:g.57605732) T>C. The allelic and genotypic frequencies for the identified SNPs in the sheep breeds were calculated. At nt positions 372 and 211, two alleles were identified (A and G). Only two genotypes were present at nt position 372 (AA and AG), while three genotypes were present at nt position 211 (AA, AG, and GG). Two alleles (T and C) and three identified genotypes (TT, TC, and CC) were detected at nt positions 143 and 111. Analysis of the results revealed that AA genotype at nt position 372 is associated with higher estimates for BW, FW, and ADG when compared to all the other genotypes. Very high correlation coefficients were found between the genotypes 143-TT and 111-TT and also between 143-TC and 111-TC. The genotypes 372-AG, 211-GG, 211-AA, 143-TT, 143-CC, 111-TT, 111-TC, and 111-CC were associated with negative effects on BW, FW, and ADG. Conclusion: The detection of four SNPs in a partial sequence of the Egyptian ovine FABP4 gene intron 1 reflected that this gene harbors substantial diversity. In addition, a novel SNP at nt position 372 (CP011894.1:g.57605471) A>G was associated with higher estimates for BW, FW, and ADG.

Association of single nucleotide polymorphisms in the insulin and growth hormone gene with growth traits of Mia Chicken

2019 ◽  
Author(s):  
Nguyen Hoang Thinh ◽  
Hoang Anh Tuan ◽  
Nguyen Thi Vinh ◽  
Bui Huu Doan ◽  
Nguyen Thi Phuong Giang ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Single Nucleotide Polymorphisms ◽  
Growth Traits ◽  
Marker Gene ◽  
Average Daily Gain ◽  
Growth Hormone Gene ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Chicken Breed ◽  
Gh Gene

This study was conducted in Mia chicken breed to evaluate the association between four single nucleotide polymorphisms (SNPs), in insulin (INS) and growth hormone (GH) genes, with growth traits. Three genotypes for the SNP A3971G of INS gene and the SNP G662A and C423T of GH gene were present in the population while only two genotypes were found in the Mia chicken breed for SNP T3737C of INS gene (TT and TC). The SNP T3737C INS gene and G662A GH gene had significant association with growth traits (P less than 0.05). A significant association of T3737C INS gene with body weight (BW) was observed at 10 to 12 weeks of age and average daily gain (ADG) at 6-8 weeks of age. The SNP G662A of the GH gene was significantly associated (P less than 0.05) with BW of Mia chicken at ages from 7 to 14 weeks and with ADG (4-6; 6-8; 8-10; 10-12 and 2-16 weeks). Chicken with the GG genotype had greater BW and ADG compared to the other genotypes. The results demonstrated that this SNP G662A GH gene may be used as a candidate marker gene for genetic improvement of growth traits in Mia chicken breed.

scholarly journals The FABP4 gene polymorphism is associated with meat tenderness in three Chinese native sheep breeds

2011 ◽  
Vol 56 (No. 1) ◽  
pp. 1-6 ◽  
Author(s):  
Q.L. Xu ◽  
G.W. Tang ◽  
.. Zhang QL ◽  
Y.K. Huang ◽  
Y.X. Liu ◽  
...  
Keyword(s):  
Meat Tenderness ◽  
Nucleotide Polymorphisms ◽  
Marbling Score ◽  
Single Nucleotide ◽  
Fatty Acid Binding ◽  
Reading Frame ◽  
Sheep Breeds ◽  
Intron 1 ◽  
Pcr Method ◽  
Native Sheep

The aim of this study was to assess the association of single nucleotide polymorphisms (SNP) of sheep fatty acid binding protein 4 (FABP4) gene with longissimus thoracis muscle (LT) meat quality traits in sheep. The FABP4 cDNA was cloned by RT-PCR method, and the sequence analysis showed that the open reading frame of sheep FABP4 is 399 bp and codes 132 amino acids. A mutation (A/G) detected in intron 1 of FABP4 gene was studied in 286 lambs of three Chinese native sheep breeds by PCR-SSCP procedure. Significant statistical association results revealed that AA genotype conferred higher tenderness (P < 0.05), muscle marbling score (P < 0.05) and intramuscular fat content (IMF; P < 0.05). Thus we suggested that the genotype AA could be regarded as a molecular marker for LT meat tenderness and IMF content in sheep.

Associated analysis of single nucleotide polymorphisms of IGF2 gene’s exon 8 with growth traits in Wuzhishan pig

2009 ◽  
Vol 37 (1) ◽  
pp. 497-500 ◽  
Author(s):  
Guanyu Hou ◽  
Dongjin Wang ◽  
Song Guan ◽  
Hongpu Zeng ◽  
Xianzhou Huang ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Growth Traits ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Wuzhishan Pig

scholarly journals Genetic diversity and genetic origin of Lanping black-boned sheep investigated by genome-wide single-nucleotide polymorphisms (SNPs)

2020 ◽  
Vol 63 (1) ◽  
pp. 193-201
Author(s):  
Heli Xiong ◽  
Xiaoming He ◽  
Jing Li ◽  
Xingneng Liu ◽  
Chaochao Peng ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Single Nucleotide Polymorphisms ◽  
Structure Analysis ◽  
Genetic Background ◽  
Nucleotide Polymorphisms ◽  
Genetic Origin ◽  
Single Nucleotide ◽  
Sheep Breeds ◽  
Genome Wide ◽  
Nj Tree

Abstract. Lanping black-boned sheep was first discovered in the 1950s in Lanping county of China and characterized by black pigmentation on skin and internal organs. Due to the novel and unique trait, the genetic background of Lanping black-boned sheep is of great interest. Here, we genotyped genome-wide SNPs (single nucleotide polymorphisms) of Lanping black-boned sheep and Lanping normal sheep using Illumina OvineSNP50 BeadChip to investigate the genetic diversity and genetic origin of Lanping black-boned sheep. We also downloaded a subset SNP dataset of two Tibet-lineage sheep breeds and four other sheep breeds from the International Sheep Genomics Consortium (ISGC) as a reference for interpreting. Lanping black-boned sheep had a lower genetic diversity level when compared to seven other sheep breeds. Principal component analysis (PCA) showed that Lanping black-boned sheep and Lanping normal sheep were clustered into the Asian group, but there was no clear separation between the two breeds. Structure analysis demonstrated a high ancestry coefficient in Lanping black-boned sheep and Lanping normal sheep. However, the two populations were separated into two distinct branches in a neighbor-joining (NJ) tree. We further evaluated the genetic divergence using population FST, which showed that the genetic differentiation that existed between Lanping black-boned sheep and Lanping normal sheep was higher than that between Tibet sheep and Changthangi sheep, which revealed that Lanping black-boned sheep is a different breed from Lanping normal sheep on the genetic level. In addition, structure analysis and NJ tree showed that Lanping black-boned sheep had a relatively close relation with Tibet sheep. The results reported herein are a first step toward understanding the genetic background of Lanping black-boned sheep, and it will provide informative knowledge on the unique genetic resource conservation and mechanism of novel breed formation.

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