scholarly journals Analysis and Comparison of Zinc Micro Element Concentration Present in Human Seminal Plasma of Asthenospermia and Normospermia Conditions Using Atomic Absorption Spectroscopy for Male Infertility Diagnosis Related to Motility Issues

2021 ◽  
Vol 36 (1) ◽  
pp. 692-697
Author(s):  
Yaddala Thanusha ◽  
A.S. Vickram

Aim: The major aim of this study is to compare Zinc (Zn) concentration present in human seminal plasma of asthenospermia (motility issues) and normospermia semen samples for identification of clinical relevance. Materials and Methods: Semen samples of normospermia (N=75), asthenospermia (N=75) were collected and semen analysis report has been done by standard world health organization protocol. Zn concentration was evaluated by using atomic absorption spectroscopy (AAS) for both the groups by using standard protocol. Results: Independent sample T-test on fertile and infertile men (asthenospermia and normospermia group) reveals that the zinc concentration is statistically insignificant (P=0.367). Zinc concentration was found to be high in normospermia (mean 2.5 mg/ml) over asthenospermia (mean 0.90 mg/ml) samples. Conclusion: Zn being an essential element for normal functioning for fertilization, its absence or less concentration may lead to infertility. We found high concentration of Zn in normospermia which reflects normal conditions for fertilization and less concentration in asthenospermia reflecting infertile conditions.

2015 ◽  
Vol 26 (1) ◽  
pp. 11-15
Author(s):  
Anna Laskowska ◽  
Danuta Wiechuła

Abstract The aim of this research was to assess the concentration of lead and zinc in chosen medicinal plants obtained from recreational areas of Katowice as well as to compare the phytoaccumulation properties of these plants. Lead and zinc concentration in plants and soil was indicated with flame and flameless atomisation atomic absorption spectroscopy AAS method. Phytoaccumulation properties of examined plant species were compared using the coefficients: enrichment factor, phytoaccumulation factor and coefficient of specific relative accumulation.


The study was carried out to assess the levels of heavy metals in ground water sampled from ten selected borehole sites in Ongata Rongai town, Kajiado County. The levels of selected heavy metals analyzed were: Zinc, Lead, Mercury, Manganese, Cadmium and Chromium in dry and wet seasons the analysis was done by Flame Atomic Absorption Spectroscopy. Seasonal variation was considered as an aspect of pollution to the subsurface environment where there was no obvious observable environmental degradation or where unknown contaminants could have been disposed of, privately, without any matrix being charged. The analysis of water samples was done using Flame Atomic Absorption Spectroscopy and the results for the metal levels were in the range of: Zinc Below Detectable Limits- 0.73±0.01 (mgl-1 ); lead 0.21±0.01- 0.33±0.01 (mgl-1 ), mercury 0.0010 ± 0.0001 - 0.0019±0.0001(mgl-1 ); 0.256±0.01 (mgl-1 ); manganese 0.03±0.01- 0.26±0.01 (mgl-1 ). Cadmium and chromium had levels below detectable limits in dry and wet seasons. The levels of zinc and manganese were higher in the dry season than those recommended by World Health Organization of 3.0mgl-1 and 0.01mgl-1 respectively. The presence of the heavy metals in the borehole water is of concern since they could impact negatively on human health even at low levels due their accumulation. Stringent management and public awareness are required in order to safeguard the environment and human health in Ongata Rongai town.


1984 ◽  
Vol 30 (8) ◽  
pp. 1366-1368 ◽  
Author(s):  
L Lampugnani ◽  
M Maccheroni

Abstract This colorimetric method for determination of zinc in seminal liquid is based on the reaction between zinc and 4-(2-pyridylazo)resorcinol in a buffered solution at pH 9.5. It is very simple, fast (approximately equal to 10 min), and economical. It can be used to determine zinc in "whole" semen, seminal plasma, or isolated spermatozoa. We verified its accuracy for sample concentrations up to 0.4 g/L by comparison with a reference method based on atomic absorption spectroscopy of acidic wet-ashed samples. Calcium and magnesium do not interfere, even in concentrations exceeding the physiological values. Between-run precision at a normal concentration of 0.14 g/L is about +/- 7 mg/L; within-run precision at the same concentration is about +/- 2 mg/L.


2018 ◽  
Vol 28 (4) ◽  
pp. 1259-1264
Author(s):  
Kiril Lisichkov ◽  
Katerina Atkovska ◽  
Neven Trajchevski ◽  
Orce Popovski ◽  
Nadica Todorovska

The presence of some chemical compounds at higher levels than maximum permissible concentrations (MPC) in the drinking water, suggests of water resources pollution. In this paper the following elements were analyzed: total arsenic, cadmium, lead, cooper and zinc. Twelve samples of water from the water supply system from the city of Skopje were examined during one year from three different springs. Also, ten samples of bottled water from three producers from the Macedonian market were tested.The determined average mass concentrations of total As, Cd(II), Pb(II), Cu(II) and Zn(II) in the analyzed water samples from the water supply system are 1.35 μg/l, 0.06 μg/l, 0.6 μg/l, 0.9 μg/l and 1,12 μg/l, respectively, and for the tested bottled water, the mean values ranges from 0.56 - 0.83 μg total As / l, 0.053 - 0.056 μg Cd(II)/l, 0.51 - 0.54 μg Pb(II)/l , 0.6 - 0.87 μg Cu(II)/l and 0.68 - 0.8 μg Zn(II)/l water.The following instrumental analytical methods and techniques were used for the analysis of the tested samples of drinking water: flame atomic absorption spectroscopy (AAS), atomic absorption spectroscopy with hydride cеll, electrothermal atomic absorption spectroscopy.The obtained results are shown in tables and graphic form. According to the obtained results a comparative analysis was carried out indicate that it is a water of good quality that can be used in different branches of the process industry.The obtained results in this paper do not exceed the values of the MPC of the Republic of Macedonia prescribed by the legal regulations for the drinking water, which confirm the health safety of the drinking water from the water supply system in the city of Skopje and the packed waters from the Macedonian market in relation to the tested elements.


Sign in / Sign up

Export Citation Format

Share Document