Additional cytogenetic abnormalities in chronic myeloid leukemia; an experience from Pakistan

Abstract Objectives: To evaluate the presence and characteristics of additional karyotype abnormalities in chronic myeloid leukaemia cases. Method: The cross-sectional study was conducted at the Department of Cytogenetics and Molecular Pathology, National Institute of Blood Diseases and Bone Marrow Transplant, Karachi, from May 2010 to September 2016 and comprised diagnosed chronic myeloid leukaemiapatients regardless of age and gender.Baseline cytogenetic evaluation was done on overnight, 24-hrs un-stimulated and 72-hrs stimulated bone marrow cultures, and karyotypes were defined according to the International System for Human Cytogenetic Nomenclature2013. Data was analysed using SPSS 23. Results: There were 222 cases with a median age of 38 years (range: 12-84 years). The male-to-female ratio was 1.8:1. Chronic myeloid leukaemiawas detected in 18(8.1%) patients havingadditional cytogenetic abnormalities. Among the patients found positive, cytogenetic type was minor in 10(55.55%), major 3(16.66%), complex 3(16.66%), and variant 2(11.11%). . Conclusion: Additional cytogenetic abnormalitieswere found in 8% of the sample. Key Words: Additional cytogenetic abnormalities, Chronic myelogenous leukaemia, Bone marrow, Cytogenetics.

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The utility of strain imaging in the cardiac surveillance of bone marrow transplant patients

Heart ◽

10.1136/heartjnl-2021-319359 ◽

2021 ◽

pp. heartjnl-2021-319359

Author(s):

Tejas Deshmukh ◽

Peter Emerson ◽

Paul Geenty ◽

Shehane Mahendran ◽

Luke Stefani ◽

...

Keyword(s):

Bone Marrow ◽

Global Longitudinal Strain ◽

Group Versus ◽

Marrow Transplant ◽

Cross Sectional Study ◽

Left Ventricular ◽

Right Ventricular Free Wall ◽

Cross Sectional ◽

Allogenic Bone ◽

Transplant Patients

ObjectiveTo evaluate the utility of two-dimensional multiplanar speckle tracking strain to assess for cardiotoxicity post allogenic bone marrow transplantation (BMT) for haematological conditions.MethodsCross-sectional study of 120 consecutive patients post-BMT (80 pretreated with anthracyclines (BMT+AC), 40 BMT alone) recruited from a late effects haematology clinic, compared with 80 healthy controls, as part of a long-term cardiotoxicity surveillance study (mean duration from BMT to transthoracic echocardiogram 6±6 years). Left ventricular global longitudinal strain (LV GLS), global circumferential strain (LV GCS) and right ventricular free wall strain (RV FWS) were compared with traditionl parameters of function including LV ejection fraction (LVEF) and RV fractional area change.ResultsLV GLS (−17.7±3.0% vs −20.2±1.9%), LV GCS (−14.7±3.5% vs −20.4±2.1%) and RV FWS (−22.6±4.7% vs −28.0±3.8%) were all significantly (p=0.001) reduced in BMT+AC versus controls, while only LV GCS (−15.9±3.5% vs −20.4±2.1%) and RV FWS (−23.9±3.5% vs −28.0±3.8%) were significantly (p=0.001) reduced in BMT group versus controls. Even in patients with LVEF >53%, ~75% of patients in both BMT groups demonstrated a reduction in GCS.ConclusionMultiplanar strain identifies a greater number of BMT patients with subclinical LV dysfunction rather than by GLS alone, and should be evaluated as part of post-BMT patient surveillence. Reduction in GCS is possibly due to effects of preconditioning, and is not fully explained by AC exposure.

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Frequency of High MELD Score in Cirrhotic Patients undergoing Liver Resection due to Hepatocellular Carcinoma

10.53350/pjmhs211592841 ◽

2021 ◽

Vol 15 (9) ◽

pp. 2841-2843

Author(s):

Muhammad Omer Farooq ◽

Niaz Ahmed ◽

Hassan Nadeem ◽

Kashif Rafi ◽

Sadia Jabbar ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Liver Resection ◽

Cross Sectional Study ◽

Meld Score ◽

Cross Sectional ◽

Female Ratio ◽

Score Study ◽

Insignificant Difference ◽

Cirrhotic Patients ◽

Male To Female

Objective: To determine the frequency of high MELD score in cirrhotic patients undergoing liver resection due to hepatocellular carcinoma also compare the frequency of mortality in patients with high or low MELD score. Study Design: Cross sectional study Place and Duration: Department of Gastroenterology, Shaikh Zayed Hospital, Lahore. Duration: 6months i.e. 23 12-2017 to 22-06-2018. Methodology: 75 patients were enrolled. Then blood sample was obtained. Reports assessed and MELD score calculated. Scores were labeled as high or low. Patients underwent liver resection according to BCLC. The mortality was noted. All the collected data was entered and analyzed on SPSS version 22. Results: In this study out of total 75 cases 60 were males and 15 females. The mean age of patients was 39.44±9.76 years, male to female ratio was 4:1. Low MELD class was noted in 45 (60%) cases and high MELD class noted in 30(40%) cases. Mortality occurred in 27(36%) cases. Insignificant difference found between the MELD class with mortality. Conclusion: High MELD score was seen in 40% cirrhotic patients undergoing liver resection due to HCC. Post HCC resection, mortality occurred in 36% patients within three months of surgery. No significant association was found between the mortality and MELD score. Keywords: MELD, Hepatocellular Carcinoma, Mortality, Cirrhosis

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HIV Prevalence and Frequency of Risk Behavior in Injecting Drug Users Entering Treatment and Out of Treatment: A Cross-Sectional Study in Five Italian Cities

Journal of Drug Issues ◽

10.1177/002204269402400312 ◽

1994 ◽

Vol 24 (3) ◽

pp. 527-535 ◽

Cited By ~ 6

Author(s):

Giovanni Rezza ◽

Stefania Salmaso ◽

Damiano Abeni ◽

Giovanna Brancato ◽

Alessandra Anemona ◽

...

Keyword(s):

Risk Behavior ◽

Drug Users ◽

Injecting Drug Users ◽

Cross Sectional Study ◽

Hiv Prevalence ◽

Sectional Study ◽

Cross Sectional ◽

Female Ratio ◽

Outreach Programs ◽

Male To Female

We conducted a study to describe current patterns of drug-related behaviors, and to identify characteristics that may distinguish injecting drug users entering treatment from those out of treatment, in five Italian cities. Overall, 1,180 subjects were recruited — 568 entering treatment and 612 out of treatment. Male to female ratio was 6.6:1. The median age was similar in the two groups. A high proportion of injecting drug users recruited out of treatment had been in treatment at least once. HIV prevalence among injecting drug users entering treatment was not higher than that of those who were out of treatment. Furthermore, a large proportion of injecting drug users who still were out of treatment reported having adopted safe behaviors. The results of the study emphasize the need to implement outreach programs aimed at harm reduction.

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Does Static Intra Access Pressure Monitoring of Arteriovenous Fistulas Predicts Intra Access Stenosis?

Pakistan Journal of Kidney Diseases ◽

10.53778/pjkd40349 ◽

2021 ◽

Vol 4 (03) ◽

Author(s):

Abaid ur Rehman ◽

Muhammad Imran Khan ◽

Omer Sabir ◽

Muhammad Mohsin Riaz ◽

Mubashar Dilawar ◽

...

Keyword(s):

Diagnostic Accuracy ◽

Vascular Access ◽

Doppler Ultrasonography ◽

Cross Sectional Study ◽

Cross Sectional ◽

Female Ratio ◽

Arteriovenous Fistulas ◽

Doppler Us ◽

Sensitivity Specificity ◽

Male To Female

The initiation of hemodialysis in patients with chronic kidney disease (CKD) requires vascular access formation. The choice of vascular access for individual patient depends on various factors however arteriovenous fistula (AVF) is conventionally considered to be the vascular access of choice. Once hemodialysis is initiated through a mature AVF, there is an ongoing need for surveillance of the AVF to ensure adequate function and prevent vascular access issues among which flow obstruction (both inflow and outflow) remains the most important. AVF stenosis can potentially lead to inadequate dialysis delivery and thrombosis thus leading to access loss. Physical examination and AVF Doppler ultrasonography (DUS) are useful for evaluation of stenosis in the arteriovenous connection and the outflow tract. Periodic assessment of the AVF with Static Intra access Pressure (SIAPR) determination may be a reliable means of predicting vascular access stenosis. Material & Methods A cross sectional study carried out at Department of Nephrology, Fatima Memorial Hospital, Lahore from July 2018 to December 2018. In total 113 patients were included, and all patients underwent SIAPR assessment and Doppler Ultrasound of AVF. Results Mean age of the patients was 56.81±9.38 years, male to female ratio of the patients was 1.8:1. In this study the SIAPR was suggestive of stenosis in 87(76.99%) patients. The sensitivity, specificity and diagnostic accuracy of SIAPR against Doppler US of the AVF for detection of stenosis was 75.86%, 22.62% & 36.28% respectively Conclusion SIAPR has low specificity and diagnostic accuracy compared to Doppler US for detection of AVF stenosis.

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Clinico-Hematological and cytogenetic spectrum of adult myelodysplastic syndrome The first retrospective cross-sectional study in Iranian patients

10.21203/rs.3.rs-249637/v1 ◽

2021 ◽

Author(s):

Mostafa Paridar ◽

Kazem Zibara ◽

Seyed Esmaeil Ahmadi ◽

Abbas Khosravi ◽

Maral Soleymani ◽

...

Keyword(s):

Myelodysplastic Syndrome ◽

Cross Sectional Study ◽

Cytogenetic Abnormality ◽

Sectional Study ◽

Trisomy 8 ◽

Cross Sectional ◽

Iron Storage ◽

Monosomy 7 ◽

Female Ratio ◽

Cytogenetic Abnormalities

Abstract Background Myelodysplastic syndrome (MDS), a heterogeneous group of hematopoietic malignancy, has been shown to present different cytogenetic abnormalities, risk factors, and clinico-hematological features in different populations and geographic areas. Herein, we determined the cytogenetic spectrum and clinico-hematological features of Iranian MDS patients for the first time. Methods This prospective cross-sectional study was conducted on 103 patients with MDS in Ahvaz, southwest of Iran, from 2014 to 2018. Clinical presentations, complete blood counts (CBC), and bone marrow (BM) biopsy samples were assessed. Perls' staining was used to evaluate BM iron storage. The cytogenetic evaluation was performed using the conventional G banding method on the BM. Results Patients’ median age was 62.3 (ranged from 50–76), and the majority were male (72.8%). The most common clinical symptom at the time of admission was fatigue (n = 33) followed by pallor (n = 27). The most common subgroup was MDS-Multi Lineage Dysplasia (MDS-MLD) (n = 38, 36.8%), followed by MDS-Single Lineage Dysplasia (MDS-SLD) (n = 28, 18.4%). A normal karyotype was observed in 59 patients (57.3%), while 44 patients (42.7%) had cytogenetic abnormalities. Trisomy 8 (+ 8) was the most common cytogenetic abnormality (n = 14) followed by dell 17p (n = 9) and monosomy 7 (-7) (n = 7). Twelve patients (11.65 %) were transformed to AML. Conclusion Our data betokened that among our MDS patients, Trisomy 8 is the predominant cytogenetic abnormality, and MDS-MLD and MDS-SLD are the most common of subtypes. Noteworthy, the male: female ratio was slightly higher in Iran than in previous reports from other parts of the world. Our study is the first report of the clinical, hematological, and cytogenetic spectrum of MDS patients in Iran

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Comparison of Led Fluorescent Microscopy and the Gene Xpert MTB/RIF Assay in Diagnosis of Pulmonary and Extrapulmonary Tuberculosis

Tribhuvan University Journal of Microbiology ◽

10.3126/tujm.v6i0.26596 ◽

2019 ◽

Vol 6 ◽

pp. 127-132

Author(s):

Sanam Thapa Magar ◽

Gokarna Ghimire ◽

Pradeep Kumar Shah

Keyword(s):

Pulmonary Tuberculosis ◽

Extrapulmonary Tuberculosis ◽

Fluorescent Microscopy ◽

Cross Sectional Study ◽

Smear Microscopy ◽

Cross Sectional ◽

Female Ratio ◽

Standard Guideline ◽

National Tuberculosis ◽

Male To Female

Objectives: The objective of this study was to evaluate Gene Xpert MTB/RIF Assay and anid fast staining (AFB) for rapid detection of Mycobacterium tuberculosis in specimen of patients suspected of pulmonary tuberculosis (PTB) and extra pulmonary tuberculosis (EPTB). Methods: A comparative cross-sectional study of 400 samples (PTB-365 and EPTB-35) of patients visiting National Tuberculosis Centre (NTC) was conducted from July 2018 to December 2018. Gene Xpert MTB/ RIF Assay, smear microscopy were performed under standard guideline inside biosafety cabinet class II. The result obtained from both the tests were analyzed using SPSS 20.0 software and Excel 2019. Results: Of the total samples, 18% (72/400) and 39% (156/400) were positive by AFB smear microscopy and Xpert MTB/RIF assay respectively. Prevalence of MTB positive was highest in the age group 35-44 years, 33 cases (17.74%) were detected in total, with a male to female ratio of 2.3:1. Pleural fluid, pus, and CSF fluid also yielded positive results with the Gene Xpert MTB/RIF assay accounting 1.28%, 0.64% and 1.28% of MTB positive case respectively. Rifampicin resistance was observed in 1.28% of the cases. Conclusion: The key findings of this study suggest that Gene Xpert test should be implemented as primary diagnostic test for PTB and EPTB.

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Pattern of Skin Diseases: Experience from a Rural Community of Bangladesh

Bangladesh Medical Journal ◽

10.3329/bmj.v41i1.18784 ◽

2014 ◽

Vol 41 (1) ◽

pp. 50-52

Author(s):

N Ahmed ◽

MZ Islam ◽

S Farjana

Keyword(s):

Rural Community ◽

Skin Diseases ◽

Ecological Factors ◽

Cross Sectional Study ◽

Cross Sectional ◽

Female Ratio ◽

Cutaneous Infections ◽

The People ◽

Disease Pattern ◽

Male To Female

Disease pattern in a given population is generally determined by different ecological factors. Thus the objective of this cross-sectional study was to determine the pattern of skin diseases in a selected rural community of Dhamrai Upazila under Dhaka district. Out of 2645 patients attending a medical camp showed 410(15.5%) patients with dermatological problems. Among all, 260(63.4%) patients were males and 150(36.6%) were females with a male to female ratio of 1.7:1. Of these patients, 178(43.4%) had cutaneous infections and 234(56.6%) had non-infectious dermatoses. Few patients (2.7%) had more than one dermatoses. Fungal infection was the commonest infection seen (22.9%) and eczemas took an upper hand in non-infectious group (32.2%). Improvement in the standard of living, health education, improvement in the environmental sanitation and good nutritious food may help the people to bring down the skin disease in the rural part of country. DOI: http://dx.doi.org/10.3329/bmj.v41i1.18784 Bangladesh Medical Journal 2012 Vol. 41 No. 1; 50-52

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NORMAL PLANTAR FASCIA THICKNESS IN ADULT

MNJ (Malang Neurology Journal) ◽

10.21776/ub.mnj.2022.008.01.4 ◽

2022 ◽

Vol 8 (1) ◽

pp. 16-20

Author(s):

Yusak Mangara Tua Siahaan ◽

Pricilla Yani Gunawan ◽

Jeffry Foraldy Haryanto ◽

Veli Sungono

Keyword(s):

Plantar Fasciitis ◽

Plantar Fascia ◽

Cross Sectional Study ◽

Heel Pain ◽

Sectional Study ◽

Normal Range ◽

Cross Sectional ◽

Female Ratio ◽

History Of ◽

Male To Female

Background: Plantar fasciitis is a common problem caused by thickening of the plantar fascia. The normal plantar fascia thickness ranged between 2-3 mm and it was generally accepted that value more than 4mm was considered pathologic. Objective: to identify normal plantar fascia thickness in adults using ultrasonography. Methods: This is a cross sectional study measuring the thickness of plantar fascia in 145 subjects with no history of heel pain. Plantar fascia thickness was measured in both feet using an ultrasound. Age, height and weight were recorded and analysed. Results: As much as 145 subjects were included in this study. Male to female ratio was 0.7. Mean age was 44 and body mass index (BMI) was mostly within normal range. Plantar fascia thickness in male was 2.71 ± 0.48 mm in right foot, and 2.74 ± 0.47 mm in left foot. Fascia thickness in female was 2.55 ± 0.50 mm in right foot, and 2.57 ± 0.45 mm in left foot. There was a significant plantar fascia thickness difference between male and female (p = 0.035 in right foot, and p=0.04 in left foot). Age, weight and BMI had a significant correlation towards plantar fascia thickness. In multivariate analysis, age and BMI revealed to have a linear correlation to plantar fascia thickness Conclusion: Age and BMI were found to be the best predictive factor of plantar fascia thickness.

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Cardiovascular function in children following bone marrow transplant: a cross-sectional study

Bone Marrow Transplantation ◽

10.1038/sj.bmt.1700600 ◽

1997 ◽

Vol 19 (1) ◽

pp. 61-66 ◽

Cited By ~ 30

Author(s):

G M Eames ◽

J Crosson ◽

J Steinberger ◽

M Steinbuch ◽

K Krabill ◽

...

Keyword(s):

Bone Marrow ◽

Bone Marrow Transplant ◽

Cardiovascular Function ◽

Marrow Transplant ◽

Cross Sectional Study ◽

Sectional Study ◽

Cross Sectional

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Demography and Etiology of Congenital Cataract in a Tertiary Eye Centre of Kathmandu, Nepal

Health Renaissance ◽

10.3126/hren.v12i1.11975 ◽

2015 ◽

Vol 12 (1) ◽

pp. 3-10

Author(s):

Ananda Kumar Sharma ◽

D N Shah ◽

M P Upadhyay ◽

M Thapa ◽

G. S. Shrestha

Keyword(s):

Congenital Cataract ◽

Cross Sectional Study ◽

Maternal Infection ◽

Cross Sectional ◽

Female Ratio ◽

Visual Status ◽

History Of ◽

Eye Examination ◽

Thyroid Profile ◽

Male To Female

Background: Congenital Cataract is the most important cause of treatable childhood blindness. Rubella is one of the major causes of preventable disease in many countries. There are scanty reports on congenital cataract in Nepal. Objective: To find out the demographic and etiological factors of congenital cataract in children. Method: In a hospital based cross sectional study, 46 children with congenital cataract were evaluated to find out morphology of cataract, laterality, associated ocular and systemic abnormality, visual status and etiology of cataract. Assessment included antenatal, birth and neonatal history, a detailed eye examination in slit lamp or the operating microscope under general anaesthesia, serum serology for TORCH infections, random blood sugar, urine reducing substance and thyroid profile. Result: Among 46 children with congenital cataract, 76.1% children presented before 5 years of age and 78.2% had bilateral onset. Male to female ratio was 1.3:1. Most of the children were legally blind (79.3%) in cataractous eye. Family history of congenital cataract was present in 15.2% cases. The most common mode of presentation was leukocoria in 91.3%. Microcornea (28.3%), resolved uveitis (13.0%), and iris atrophy (8.7%) were the most common ocular associations. Delayed developmental milestone (21.7%) and cardiac anomalies (10.9%) were the most common systemic anomalies. Lamellar cataract (51.3%) was the most common morphology of cataracts observed. The maternal infection was the major cause of congenital cataract in 17.4% cases with predominantly rubella infection in 13% cases. Conclusion: Most of the children with cataract are legally blind. Maternal infection in the antenatal period is the major cause of congenital cataract.DOI: http://dx.doi.org/10.3126/hren.v12i1.11975 Health Renaissance 2014;12(1):3-10

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