MOLECULAR MARKING OF POPULUS SPECIES AND HYBRIDS FOR THE NEEDS OF BREEDING AND EXPERIMENTAL BOTANICS

Abstract Populus deltoides, P. nigra, and P. trichocarpa are the most important species for poplar breeding programs worldwide. In addition, Populus has become a model for fundamental research on trees. Linkage maps were constructed for these three species by analyzing progeny of two controlled crosses sharing the same female parent, Populus deltoides cv. S9-2 × P. nigra cv. Ghoy and P. deltoides cv. S9-2 × P. trichocarpa cv. V24. The two-way pseudotestcross mapping strategy was used to construct the maps. Amplified fragment length polymorphism (AFLP) markers that segregated 1:1 were used to form the four parental maps. Microsatellites and sequence-tagged sites were used to align homoeologous groups between the maps and to merge linkage groups within the individual maps. Linkage analysis and alignment of the homoeologous groups resulted in 566 markers distributed over 19 groups for P. deltoides covering 86% of the genome, 339 markers distributed over 19 groups for P. trichocarpa covering 73%, and 369 markers distributed over 28 groups for P. nigra covering 61%. Several tests for randomness showed that the AFLP markers were randomly distributed over the genome.

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Development of mitochondrial SNP markers in different Populus species

Trees ◽

10.1007/s00468-014-1136-5 ◽

2014 ◽

Vol 29 (2) ◽

pp. 575-582 ◽

Cited By ~ 4

Author(s):

Birgit Kersten ◽

Mina Merle Voss ◽

Matthias Fladung

Keyword(s):

Snp Markers ◽

Populus Species

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Response of three Populus species to drought.

10.2737/nc-rp-302 ◽

1991 ◽

Cited By ~ 3

Author(s):

Terry Strong ◽

Edward A. Hansen

Keyword(s):

Populus Species

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Peer Review #2 of "Characterization of the complete chloroplast genomes of five Populus species from the western Sichuan plateau, southwest China: comparative and phylogenetic analyses (v0.1)"

10.7287/peerj.6386v0.1/reviews/2 ◽

2019 ◽

Keyword(s):

Peer Review ◽

Southwest China ◽

Phylogenetic Analyses ◽

Western Sichuan ◽

Chloroplast Genomes ◽

Populus Species

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Inheritance and linkage of isozymes in Populus tremuloides (Michx.)

Genome ◽

10.1139/g87-066 ◽

1987 ◽

Vol 29 (2) ◽

pp. 384-388 ◽

Cited By ~ 6

Author(s):

Jung O. Hyun ◽

Om P. Rajora ◽

Louis Zsuffa

Keyword(s):

Populus Tremuloides ◽

Goodness Of Fit ◽

Single Gene ◽

Gene Control ◽

Glutamate Oxaloacetate Transaminase ◽

Chi Square ◽

Banding Patterns ◽

Phosphogluconate Dehydrogenase ◽

Goodness Of Fit Tests ◽

Populus Species

Progenies of four controlled crosses were assayed electrophoretically to determine the inheritance of isozymes of 10 loci coding for six enzymes, aconitase (ACO), glutamate oxaloacetate transaminase (GOT), isocitrate dehydrogenase (IDH), phosphoglucomutase (PGM), 6-phosphogluconate dehydrogenase (6-PGD), and phosphoglucose isomerase (PGI), in roots of Populus tremuloides. Chi-square goodness of fit tests verified a single-gene Mendelian control of the segregating allozyme variants at each of five loci: Aco-1, Got, Pgm-2, 6-Pgd-2, and Pgi-2. Evidence was also obtained for a single-gene control of each of the remaining five loci (Aco-2, Idh, Pgm-1, 6-Pgd-1, and Pgi-1). ACO and PGM showed monomeric, while GOT, IDH, 6-PGD, and PGI had dimeric, banding patterns. The results of joint two-locus segregation tests indicated no linkage between 6-Pgd-2 and Pgi-2. Key words: Populus species, electrophoresis, allozymes, inheritance, linkage.

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