Influence of Blood Cervical Exudative Cell Specimens on Human Papillomavirus DNA Test Results

The biological importance of human papillomavirus (HPV) in the field of medicine – related to cervical carcinogenesis – has been extensively reported in the last decades. For the first time, a direct correlation between cause and effect to explain a cancer development was completely achieved in medical research. Consequently, the Nobel Prize was awarded to HZ Hausen in 2008 for his efforts to understand the effects of persistent infection of oncogenic types of HPV and malignancy transformation. The aim of the present review was to summarize the principal elements of HPV characteristics and their importance in oncology.

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Detection of Human Papillomavirus DNA by AffiProbe HPV-DNA Test Kit in Cervical Scrapes or Biopsies-Histopathologic Correlates

Infectious Diseases in Obstetrics and Gynecology ◽

10.1155/s1064744994000517 ◽

1994 ◽

Vol 2 (3) ◽

pp. 126-129

Author(s):

Pekka Nieminen ◽

Tarja Jalava ◽

Arja Kallio ◽

Marjut Ranki ◽

Jorma Paavonen

Keyword(s):

Human Papillomavirus ◽

Dna Test ◽

Hpv Dna ◽

Test Kit ◽

Histopathologic Diagnosis ◽

Hybridization Test ◽

Hpv Types ◽

Working Day ◽

Intraepithelial Lesions ◽

Papillomavirus Dna

Objective: The aim of this study was to evaluate and compare the efficacy of punch biopsies and cervical scrapes in the detection of human papillomavirus (HPV) DNA from the cervix and compare the results with the histopathologic diagnosis.Methods: The specimens were collected simultaneously, and HPV DNA was detected using a liquid hybridization test.Results: Biopsies and scrapes were equally efficient, but each detected only two-thirds of all HPV-DNA-positive patients. Thus, the positivity rate increased when both tests were used. Overall, 13% of patients with normal histopathology, 38% of patients with benign atypia, and 66% of patients with squamous intraepithelial lesions (SIL) were HPV-DNA positive. HPV-DNA 16 was found in 54% of HPV-DNA-positive patients with SIL, in 20% of HPV-DNA-positive patients with atypia, and in none of patients with normal histopathology.Conclusions: The liquid hybridization test used in this study detects HPV DNA equally efficiently from both biopsies and scrapes. The test can be performed in 1 working day. However, the sensitivity of the test is low, and it only detects a limited number of HPV types.

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Effectiveness of a simple rapid human papillomavirus DNA test in rural Nigeria

International Journal of Cancer ◽

10.1002/ijc.27563 ◽

2012 ◽

Vol 131 (12) ◽

pp. 2903-2909 ◽

Cited By ~ 34

Author(s):

Julia C. Gage ◽

Kayode O. Ajenifuja ◽

Nicolas Wentzensen ◽

Akinfolarin C. Adepiti ◽

Mark Stoler ◽

...

Keyword(s):

Human Papillomavirus ◽

Dna Test ◽

Rural Nigeria ◽

Papillomavirus Dna

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Guidelines for human papillomavirus DNA test requirements for primary cervical cancer screening in women 30 years and older

International Journal of Cancer ◽

10.1002/ijc.24010 ◽

2009 ◽

Vol 124 (3) ◽

pp. 516-520 ◽

Cited By ~ 365

Author(s):

Chris J.L.M. Meijer ◽

Johannes Berkhof ◽

Philip E. Castle ◽

Albertus T. Hesselink ◽

Eduardo L. Franco ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Cancer Screening ◽

Cervical Cancer Screening ◽

Dna Test ◽

Test Requirements ◽

Papillomavirus Dna ◽

Primary Cervical Cancer Screening

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Statement on human papillomavirus DNA test utilization

Cancer Cytopathology ◽

10.1002/cncy.20031 ◽

2009 ◽

Vol 117 (3) ◽

pp. 154-156 ◽

Cited By ~ 3

Author(s):

Diane Solomon ◽

Jacalyn L. Papillo ◽

Diane D. Davey ◽

Keyword(s):

Human Papillomavirus ◽

Dna Test ◽

Papillomavirus Dna

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Correlation of Histopathologic Follow-up Findings With Vaginal Human Papillomavirus and Low-Grade Squamous Intraepithelial Lesion Papanicolaou Test Results

Archives of Pathology & Laboratory Medicine ◽

10.5858/arpa.2010-0658-oa ◽

2011 ◽

Vol 135 (12) ◽

pp. 1545-1549 ◽

Cited By ~ 11

Author(s):

Mona Bansal ◽

R Marshall Austin ◽

Chengquan Zhao

Keyword(s):

Human Papillomavirus ◽

Low Grade ◽

Hpv Testing ◽

Test Results ◽

Squamous Intraepithelial Lesion ◽

Papanicolaou Test ◽

Dna Test ◽

Correlation Data ◽

Intraepithelial Lesion

Context.—Data on high-risk human papillomavirus (hrHPV) DNA test results in vaginal, liquid-based cytology (LBC) specimens and corresponding cytologic and histopathologic correlation data are limited. Objective.—To analyze follow-up correlation data associated with vaginal (after hysterectomy) low-grade squamous intraepithelial lesion (LSIL) LBC and hrHPV test results. Design.—Hospital records were searched for vaginal LSIL LBC and hrHPV results between July 1, 2005, and July 30, 2009. Histopathologic and Papanicolaou test follow-up correlation data were analyzed. Results.—During the study period, 2892 patients with test results from both posthysterectomy vaginal LBC and hrHPV were identified: 148 (5.1%) of the patients had vaginal Papanicolaou test results reported as LSIL, with hrHPV detected in 113 of the 148 patients (76.4%). Of 148 patients, 59 of those with vaginal LSIL including 48 (81.4%) with positive HPV testing and 11 (18.6%) with negative HPV testing results had a follow-up vaginal biopsy. Histopathologic vaginal intraepithelial neoplasia (VAIN) 2/3 was diagnosed in 7 of 59 patients (11.9%) with biopsies. In all 7 patients who were diagnosed with VAIN 2/3, hrHPV was detected in the LBC vial. No VAIN 2/3 diagnoses were documented in the biopsy specimens from the 11 patients with hrHPV− LSIL Papanicolaou test results. Histopathologic VAIN 2/3 was diagnosed from vaginal biopsies in 7 of the 48 patients (14.6%) with vaginal hrHPV+ LSIL test results. Conclusions.—Sensitivity and specificity of hrHPV test results associated with histopathologic follow-up diagnoses of VAIN 2/3 in patients with vaginal LSIL results were 100% and 21.2%, respectively. The positive predictive value of a vaginal hrHPV+ LSIL result for a subsequent histopathologic VAIN 2/3 diagnosis was 14.6%. No cases of VAIN 2/3 were diagnosed in the 11 patients with vaginal hrHPV− LSIL results. Correlations of vaginal cytologic, histopathologic, and human papillomavirus findings were quite similar to correlation findings previously reported in older women with cervical LSIL test results.

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