Effect of Drug Sensitivity Test in the Treatment of Drug-resistant Tuberculosis

2021 ◽  
Keyword(s):  
Drug Sensitivity ◽  
Sensitivity Test ◽  
Drug Resistant ◽  
Drug Resistant Tuberculosis ◽  
Resistant Tuberculosis ◽  
Drug Sensitivity Test

PD64 Diagnostic Accuracy Of The Nitrate Reductase Assay Technique

2018 ◽  
Vol 34 (S1) ◽  
pp. 153-153
Author(s):  
Dalila Gomes ◽  
Betânia Leite ◽  
Fabiana Araujo Figueiredo da Mata
Keyword(s):  
Nitrate Reductase ◽  
Drug Sensitivity ◽  
Sensitivity Test ◽  
Drug Resistant ◽  
Drug Resistant Tuberculosis ◽  
Resistant Tuberculosis ◽  
Drug Sensitivity Test ◽  
Conventional Drug ◽  
Time To Diagnosis ◽  
Nitrate Reductase Assay

Introduction:The conventional drug sensitivity test is traditionally used in Brazil to diagnose drug-resistant tuberculosis. However, the test can take up to 60 days to return a diagnosis, which is considered too long for certain vulnerable populations. Therefore, this study analyzed the available scientific evidence on the accuracy and time to diagnosis of the nitrate reductase assay for diagnosing resistant tuberculosis, compared with the conventional drug sensitivity test.Methods:We searched MEDLINE, Embase, and The Cochrane Library for systematic reviews with meta-analyses. The articles were screened by title and abstract. The full-texts of potentially relevant articles were then screened according to the inclusion criteria.Results:Three systematic reviews with meta-analyses were selected that compared the nitrate reductase assay with the conventional drug sensitivity test. The accuracy of the nitrate reductase assay was satisfactory in most of the results when compared with the sensitivity test, except for one study that showed low sensitivity for the detection of streptomycin resistance. In addition, the nitrate reductase assay had a shorter time to diagnosis than the drug sensitivity test.Conclusions:The results of this study reinforce the idea that the nitrate reductase assay may diagnose drug-resistant tuberculosis earlier than the conventional drug sensitivity test and be a helpful strategy for controlling the disease, especially in vulnerable populations that are more likely to be affected by tuberculosis. For a broader analysis of the benefit of the assay, it is suggested that studies investigate the impact of the shorter time to diagnosis on morbidity and mortality in patients with drug-resistant tuberculosis. In addition, economic analyses comparing the nitrate reductase assay with the sensitivity test are recommended to evaluate the cost-benefit ratio.

A Preliminary Study on the Synergistic Effect of Nano Ag and Anti-Tuberculosis Drugs on Drug-Resistant Mycobacterium tuberculosis

2020 ◽  
Vol 20 (10) ◽  
pp. 6155-6160
Author(s):  
Xiaofei Li ◽  
Lina Sheng ◽  
Juncai Tu ◽  
Lianqing Lou
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Drug Sensitivity ◽  
Bactericidal Effect ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Tubercle Bacillus ◽  
Drug Resistant Tuberculosis ◽  
Nano Silver ◽  
Resistant Tuberculosis ◽  
Drug Sensitivity Test

To determine the bactericidal effect of nano silver and anti-tuberculosis drugs on drug-resistant Mycobacterium tuberculosis. Solid-state drug sensitivity tests with streptomycin (SM), isoniazid (INH), RIF, EMB, kanamycin (km), and ofloxacin (ofx) were carried out on H37Rv and different clinical isolates. Briefly, the effects of SM, INH, RIF, EMB (1.0 μg/mL, 0.1 μg/mL, 1.0 μg/mL, 5.0 μg/mL), and anti-tuberculosis drugs, and their combined effect with 0.15 μg/mL and 0.30 μg/mL nano silver on drug-resistant tuberculosis bacteria were evaluated by liquid drug sensitivity test. At the low concentration (0.15 μg/mL), nano silver could not effectively kill the tuberculosis strains; however, at concentrations ≥0.30 μg/mL, it could effectively kill H37Rv and the clinical isolates of the sensitive, single-resistant, multi-resistant, multi-resistant, and extensively resistant strains. Combining 0.15 μg/mL nano silver with the four first-line anti-tuberculosis drugs could not effectively kill the tuberculosis strains; however, combining 0.30 μg/mL nano silver and the anti-tuberculosis drugs could effectively kill the drug-resistant tuberculosis strains. Nano silver exhibits a concentrationdependent killing effect on tubercle bacillus. Further, a nano silver concentration higher than 0.30 μg/mL could kill sensitive and resistant tubercle bacillus.

scholarly journals The Potential Impact of Up-Front Drug Sensitivity Testing on India’s Epidemic of Multi-Drug Resistant Tuberculosis

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0131438 ◽  
Author(s):  
Kuldeep Singh Sachdeva ◽  
Neeraj Raizada ◽  
Radhey Shyam Gupta ◽  
Sreenivas Achuthan Nair ◽  
Claudia Denkinger ◽  
...  
Keyword(s):  
Drug Sensitivity ◽  
Drug Resistant ◽  
Sensitivity Testing ◽  
Drug Resistant Tuberculosis ◽  
Drug Sensitivity Testing ◽  
Resistant Tuberculosis ◽  
Multi Drug Resistant Tuberculosis ◽  
Potential Impact

scholarly journals Socio-demographic Profile and Drug Sensitivity Pattern of Suspected Drug Resistant Tuberculosis among Patients in Regional Tuberculosis Reference Laboratory (R.T.R.L) of a Tertiary Hospital

2017 ◽  
Vol 18 (2) ◽  
pp. 62-67 ◽  
Author(s):  
Ummay Fatema Khatun ◽  
Robed Amin ◽  
Muna Islam ◽  
Abdur Rob ◽  
Abdur Rahim
Keyword(s):  
Drug Resistance ◽  
Drug Sensitivity ◽  
Reference Laboratory ◽  
Drug Resistant ◽  
Suspected Drug ◽  
Drug Resistant Tuberculosis ◽  
Demographic Profile ◽  
Resistant Tuberculosis ◽  
Sensitivity Pattern ◽  
Mdr Tb

Background: Drug resistant tuberculosis has been reported in all regions of the world. In this study we address the socio-demographic profile and drug sensitivity pattern as well as prevalence of drug resistance tuberculosis in a tertiary center (regional TB reference laboratory) in Bangladesh.Method: This Study was carried out in R.T.R.L. (Regional TB Reference Laboratory) in 250 bedded Chittagong General Hospital. Patients who were referred to R.T.R.L during the period July 2012 to July 2013 were included in the study. Total 100 patients with suspected drug resistant tuberculosis (TB) who had any one of 9 criteria of NTP (National Tuberculosis Control Programme) were selected consecutively. Gene xpert MTB/RIF (Rifampicin resistance) test for all sputum positive cases were performed. Sputum sample of Patients with positive microscopy for AFB or positive Xpert/MTB was sent for culture. The samples with positive sputum culture were sent for drug sensitivity test for 1st line anti- tubercular drug.Result: Among 100 patients 78 were male and 22 were female, majority of the patients (64) were between 15-45 years with poor socio economic condition (73%) and primarily educated. Analysis of our Study result showed that 18% of patients were mono-drug resistant. Among them 13% showed resistance to isoniazid (INH), 4% to streptomycin and only 2% to rifampicin. No patient was found resistant to pyrazinamide (PNZ) and 38% of patient with suspected drug – resistant TB was found to have no drug resistance. 18% of patient had multidrug resistant tuberculosis (MDR-TB) among which 56% were relapse cases (48% after cat -I, 8% after cat II), 24% were non – converter of cat –I, 12% belonged to failure of cat –I, 3% failure of cat –II, 2% return after default and others. 1% of patient had history of contact with MDR –TB patient.Conclusion: Drug-resistance tuberculosis especially MDR-TB, was higher in patients with previously incomplete anti-tuberculosis treatment. A high level of drug resistance among the re-treatment TB patients poses a threat of transmission of resistant strains to susceptible persons in the community. For this reason proper counseling of patients and attention towards the completion of the anti-TB treatment are needed.J MEDICINE July 2017; 18 (2) : 62-67

scholarly journals Status of antituberculosis drug resistance in Saudi Arabia 1979-98

2021 ◽  
Vol 8 (4-5) ◽  
pp. 664-670
Author(s):  
K. K. Abu Amero
Keyword(s):  
Saudi Arabia ◽  
Drug Sensitivity ◽  
National Policy ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
First Line ◽  
Sensitivity Testing ◽  
Drug Resistant Tuberculosis ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis

All published material on the prevalence of drug-resistant tuberculosis within Saudi Arabia over the period 1979-98 was reviewed. The prevalence of single-drug-resistant tuberculosis ranged from 3.4% to 41% for isoniazid, 0% to 23.4% for rifampicin, 0.7% to 22.7% for streptomycin and 0% to 6.9% for ethambutol. The prevalence of multidrug-resistant tuberculosis [defined by WHO as resist1qance to two or more first-line antituberculosis drugs] ranged from 1.5% to 44% in different regions. No strong conclusions could be drawn owing to variations in the populations studied, geographical origins, site of Mycobacterium tuberculosis isolation [pulmonary or extrapulmonary] and drug sensitivity testing. However, the need to develop a standardized national policy for surveillance of drug-resistant tuberculosis in Saudi Arabia is clear

scholarly journals Drug resistance profile among multi-drug resistant tuberculosis patients at diagnosis and correlation with history of anti-tubercular treatment in a tertiary care center

2019 ◽  
Vol 6 (6) ◽  
pp. 1918
Author(s):  
Rahul Kumar ◽  
Rajiv Garg ◽  
Silpa Kshetrimayum ◽  
Amita Jain
Keyword(s):  
Drug Resistance ◽  
Drug Sensitivity ◽  
Drug Resistant ◽  
Second Line ◽  
Drug Resistant Tuberculosis ◽  
Resistant Tuberculosis ◽  
Extensively Drug Resistant ◽  
Primary Drug Resistance ◽  
Mdr Tb ◽  
History Of

Background: Drug Resistant Tuberculosis (DR-TB) is a major threat to the realization of the goal of a TB free world in the near future. It is important to study the reasons for the increasing number of such cases so that effective action can be taken to control this growing epidemic.Methods: Sputum from 36 patients diagnosed with acquired pulmonary Multidrug Resistant Tuberculosis (MDR-TB) were subjected to first- and second-line Drug Sensitivity Testing (DST) after liquid culture in mycobacterium growth Indicator Tube (MGIT). Primary MDR-TB cases were excluded. The relation of the drug sensitivity profile with the history of prior treatment taken was statistically analysed.Results: Majority of the patients had received appropriate treatment, and most had adhered to prescribed treatment. Among the 36 patients, 24(66.7%) were found to be Pre-Extensively Drug Resistant (Pre-XDR-TB) and 4(11.1%) were extensively drug resistant XDR-TB cases. Inappropriate prescription of fluoroquinolone (FQ) was found to be most common. Prior intake of any drug was not found to significantly affect subsequent resistance to that drug.Conclusions: Fluoroquinolone resistance is quite common in patients with DR-TB (66.7%). This study did not find the prior use of FQ or any other drug to significantly affect subsequent resistance to the drug. Primary drug resistance is thus a major concern. 11.1% patients were found to be XDR-TB cases. Hence DST for first- and second-line drugs should be done at the time of diagnosis to avoid failure of treatment with a predesigned regimen.

scholarly journals A Methylene Blue Assisted Electrochemical Sensor for Determination of Drug Resistance of Escherichia coli

2021 ◽  
Vol 9 ◽  
Author(s):  
Rongshuai Duan ◽  
Xiao Fang ◽  
Dongliang Wang
Keyword(s):  
Escherichia Coli ◽  
Methylene Blue ◽  
Drug Sensitivity ◽  
Bacterial Resistance ◽  
Animal Husbandry ◽  
Sensitivity Test ◽  
Test Method ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Drug Sensitivity Test

Due to the abuse of antibiotics in clinical, animal husbandry, and aquaculture, drug-resistant pathogens are produced, which poses a great threat to human and the public health. At present, a rapid and effective drug sensitivity test method is urgently needed to effectively control the spread of drug-resistant bacteria. Using methylene blue as a redox probe, the electrochemical signals of methylene blue in drug-resistant Escherichia coli strains were analyzed by a CV method. Graphene ink has been used for enhancing the electrochemical signal. Compared with the results of the traditional drug sensitivity test, we proposed a rapid electrochemical drug sensitivity test method which can effectively identify the drug sensitivity of Escherichia coli. The sensitivity of four E. coli isolates to ciprofloxacin, gentamicin, and ampicillin was tested by an electrochemical drug sensitivity test. The respiratory activity value %RA was used as an indicator of bacterial resistance by electrochemical method.

Sign in / Sign up

Export Citation Format

Share Document