Validated RP-HPLC Method for Simultaneous Estimation of Domperidone and
Naproxen in Bulk Drug and Formulations
A new , precise , accurate and reproducible RP – HPLC method for simultaneous estimation of bulk and pharmaceutical formulations . Separation of Naproxen and Domperidone was successfully achieved. THERMO HYPERSIL , C 18 , 250mm X 4.6mm , 5μm , or equivalent in an isocratic mode utilizing Acetonitrile : Methanol : Water (20: 60:20 ) at a flow rate of 1.0 mL/ min and elute was monitored at 284nm , with a retention time of 2.341 and 5.225 minutes for Naproxen and Domperidone respectively. The method was validated and the response was found to be linear in the drug concentration range of 1μg /mL to 6μg/mL. The values of correlation coefficient was found to be 0.988 for Naproxen and 0.998 for Domperidone respectively. The LOD and LOQ for Naproxen was found to be 0.56, 1.71 respectively . The LOD and LOQ for Domperidone was found to be 1.49 , 4.5 respectively. This method was found to be good percentage recovery for Naproxen and Domperidone were found to be 98% and 101.6% respectively indicates that the proposed method was highly accurate. The specificity of method shows good correlation between retention times of standard with the sample. The method was extensively validated according to ICH guidelines for Linearity , Accuracy , Precision , Specificity , and Robustness .