Tampon Derişiminin Biyosensör Cevabına Etkisi

2019 ◽  
pp. 8-11

Objectives: All enzymes have a specific optimum pH value. Buffer solutions are used for optimal pH value in enzyme studies. Buffer concentrations play a key role in enzymatic systems. Material and Methods: In order to examine the effects of working cell buffer concentration used in enzyme-based biosensors on biosensor response, changes in the system's response to the same standard solution were studied using buffers in different concentrations in the biosensor system that determined ferric iron. Results: In electrochemical studies, it has been observed that changing the buffer concentration can change the results of other applications. Conclusions: In electrochemical studies, optimum buffer concentration is essential to obtain healthy results. Keywords: Buffer concentration, Biosensor, Iron

Author(s):  
EMINE KARAKUS ◽  
CISEM TURAN

Objective: The objective of this study was to develop a new biosensor system based on nanoparticle to determine penicillin in pharmaceuticals. Methods: The characterization and optimization of the potentiometric penicillin biosensor (PB) were prepared by using synthesized surface-dependent and surface-independent ZnO nanoparticles named ZnO nanorods and chitosan were carried out. It was preferred ZnO nanorod because of its electrical, optical, physical and photocatalyst properties, biocompatibility and non-toxicity in the construction of the penicillin biosensor. Results: The operating range was obtained as 10-1-10-3M, the optimum buffer concentration was 10 mmol, optimum pH was 7.4 and the optimum temperature was 25 °C for the PB. The PB has advantages in terms of short response time, long enough shelf life, cheap, and easy elaborate. Conclusion: Whether the biosensor can be used to determine penicillin and accurately measure penicillin, the amount of penicillin in a commercial pharmaceutical preparation named Alfoxil was successfully made by using our prepared penicillin biosensor.


2018 ◽  
Vol 6 (2) ◽  
pp. 137-141
Author(s):  
Suman Lal Shrestha

The adsorption capacity of ferrous ions onto bio-sorbents prepared from two different types of bio-waste of banana peel (CABP) and sawdust (CASD) treated with sulfuric acid was studied using Langmuir and Freundlich isotherms. Result shows that the optimum pH value for about 99 % Fe (II) adsorption onto the CABP and CASD was found to be 3 and 4, respectively, after 5 hours or more contact time period. Both the Langmuir and Freundlich adsorption models are fitted to remove the Fe (II) ions from aqueous solution by the bio-adsorbent of CABP and CASD. The maximum adsorption capacities for the CABP and CASD were found to be about 34 and 116 mg/g, respectively. These results showed that the CASD seems to be more effective bio-adsorbent than the CABP to remove the Fe (II) ions from drinking or/and wastewaters.Int. J. Appl. Sci. Biotechnol. Vol 6(2): 137-141 


2016 ◽  
Vol 81 (12) ◽  
pp. 1407-1414 ◽  
Author(s):  
Dragan Veselinovic ◽  
Zoran Velikic

Measurements of pH values of buffer solutions (pH 4.0, 7.0 and 10.0) and distilled water have been performed with a glass electrode in electromagnetic field at the frequency interval of 10 MHz to 200 MHz and the output power of dispersed and reflected electromagnetic radiation of 0.01 W to 3 W. In all the cases, there occurred a reduction of pH values, i.e. a "recorded pH value" was obtained. The reduction appears within the applied frequency interval reaching extreme values at specific frequencies. The reduction of the pH values increases with the radiation power and depend of the solution buffer capacity. The effect of electromagnetic field on pH value change is exerted dominantly through the influence on glass electrodes.


2021 ◽  
Vol 900 (1) ◽  
pp. 012003
Author(s):  
M Balintova ◽  
Z Kovacova ◽  
S Demcak ◽  
Y Chernysh ◽  
N Junakova

Abstract Removal of heavy metals from the environment is important for living beings. The present work investigates the applicability of the natural and MnO2 - coated zeolite as sorbent for the removal of copper from synthetic solutions. Batch experiments were carried out to identify the influence of initial pH and concentration in the process of adsorption. A maximum removal efficiency of Cu(II) was observed in 10 mg/L for natural (95.6%) and modified (96.4%) zeolite, where the values was almost identical, but at concentration of 500 mg/L was the removal efficiency of modified zeolite three times higher. Based on the correlation factors R2, the Langmuir isotherms better describe the decontamination process than Freundlich. The optimum pH value was set at 5.0.


2012 ◽  
Vol 454 ◽  
pp. 305-309 ◽  
Author(s):  
Ping Chen ◽  
Xian Jun Lu ◽  
Jun Qiu

Using Octadecyltrimethylammonium cations with long carbon chain to modify calcium montmorillonite samples, the adsorption properties of different dyes on montmorillonite before and after modification were investigated. Results showed that the basic dye of methylene blue was easily adsorbed by natual calcium montmorillonite, while the adsorption ability for acid blue and neutral blue was greatly improved when it was organically modified, and the adsorption amount increased with the increasing of modifier dosage. The adsorption ratio was influnced by reaction time, the temperature and the solution’s pH value. It suggested that the adsorption balance time was about 60min; higher temperature was good for acid blue adsorbed on organic montmorillonite, but neutral blue’s adsorption amount was greater at the room temperature; the optimum pH was 9 for methylene blue adsorption, while low pH was better for acid blue and neutral blue adsorption.


2013 ◽  
Vol 807-809 ◽  
pp. 1343-1346
Author(s):  
Yi Fan Li ◽  
Ying Liu ◽  
Hou Qi Liu ◽  
Li Li

The research used activated carbon fiber (ACF) as adsorbent to remove atrazine, a kind of herbicide. It set a series of static adsorption experiments under different solution temperature, pH value and initial concentration to get a optimum adsorption condition. The experiment shows that the optimum pH for the removal of atrazine is 7. The adsorption rate is highest at 20°C. The adsorption rate decreases while the initial concentration increases.


2015 ◽  
Vol 1109 ◽  
pp. 253-256 ◽  
Author(s):  
M.A. Farehanim ◽  
U. Hashim ◽  
Norhayati Soin ◽  
A.H. Azman ◽  
S. Norhafiezah ◽  
...  

The electrical performances of silicon dioxide-based Interdigitated electrodes (IDEs) as biosensor were developed. The IDEs was made up by two individually addressable Interdigitated comb-like finger structure have frequently been suggested as a biosensor which promises higher sensitivity compared to conventional parallel electrodes. The purpose of this paper was to investigate the capacitance test and impedance test to taken with various pH solution to observe the response of the sensor with different pH values. Purchased pH buffer solutions which varied from pH2 to pH10 are dropped on the microelectrode and the effect on it is investigated for the application in pH measurement. This research has proven that increase in pH value from acidic to alkaline is proportional with capacitance. The measured values of capacitance with respect to each pH concentrations applied during the measurements were repeatable and reproducible.


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