scholarly journals Ambient temperature drying of therapeutic protein solution with use of microwave

2018 ◽  
Author(s):  
Takaharu Tsuruta ◽  
Takuma Ogawa ◽  
Ryosuke Abe ◽  
Hirofumi Tanigawa
Keyword(s):  
Ambient Temperature ◽  
Protein Conformation ◽  
Freeze Drying ◽  
Protein Structures ◽  
Egg White ◽  
Therapeutic Protein ◽  
Vacuum Drying ◽  
Protein Solution ◽  
Drying Time ◽  
Microwave Vacuum Drying

High quality drying of therapeutic protein-solution is important in medical and pharmaceutical processing.  Freeze-drying is mostly used, but it takes a long drying-time and causes damages of protein structures.  In order to improve the drying quality, we propose a microwave vacuum drying performed at ambient temperatures under low-pressure conditions.  We are focusing on the Parma-Zyme method for the evaporative drying of protein solutions such as egg white or lysozyme with vitrification. Circular dichroism (CD) spectroscopy is used to detect protein conformation changes due to the drying, and it is found that the ambient temperature drying can preserve the protein conformation. Keywords: Microwave vacuum drying; Freeze-drying; Therapeutic protein; Egg white; Lysozyme 

A Combination of Freeze Drying and Microwave Vacuum Drying of Duck Egg White Protein Powders

2014 ◽  
Vol 32 (15) ◽  
pp. 1840-1847 ◽  
Author(s):  
Bing Zhou ◽  
Min Zhang ◽  
Zhongxiang Fang ◽  
Yaping Liu
Keyword(s):  
Freeze Drying ◽  
Egg White ◽  
Vacuum Drying ◽  
Egg White Protein ◽  
Microwave Vacuum Drying ◽  
Duck Egg

The Influence of Selected Drying Methods on the Physical Properties of Dried Apples cv. Jonagold Grown in Different Locations in Europe

2017 ◽  
Vol 13 (6) ◽  
Author(s):  
Anna Michalska ◽  
Krzysztof Lech ◽  
Adam Figiel ◽  
Grzegorz P. Łysiak
Keyword(s):  
Physical Properties ◽  
Freeze Drying ◽  
Geographical Origin ◽  
Raw Material ◽  
Convective Drying ◽  
Vacuum Drying ◽  
Drying Methods ◽  
Drying Time ◽  
Microwave Vacuum Drying ◽  
Apple Slices

Abstract The aim of the study was to determine the influence of four different growing locations of apples cv. ‘Jonagold’ in Europe on the drying kinetics and the physical properties of dried apple slices. The drying methods applied in the study (freeze-drying, convective drying, microwave-vacuum drying and combined drying) significantly affected the drying time, which was the shortest in the case of microwave vacuum drying. The geographical origin of the apples affected the chemical and physical properties of the raw material used for drying, and, consequently, the drying time. Water activity of dried samples was connected with the final dry matter, regardless of the geographical origin of the apples. Freeze-drying resulted in the lightest in colour products (L*), whereas microwave-vacuum dried products had the highest levels of yellow pigments (b*). The highest chroma and browning index values were noted for microwave-vacuum dried samples and were strongly influenced by the drying temperature. The mechanical properties of the apple slices were more dependent on the drying method and temperature of the process than on the geographical origin when the temperature exceeded 83 °C.

scholarly journals Evaluation of Microwave Vacuum Drying as an Alternative to Freeze-Drying of Biologics and Vaccines: the Power of Simple Modeling to Identify a Mechanism for Faster Drying Times Achieved with Microwave

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Akhilesh Bhambhani ◽  
Justin Stanbro ◽  
Daniel Roth ◽  
Elizabeth Sullivan ◽  
Morrisa Jones ◽  
...  
Keyword(s):  
Freeze Drying ◽  
Drug Product ◽  
Scale Up ◽  
Heat Transfer Process ◽  
Vacuum Drying ◽  
Drying Process ◽  
Drying Time ◽  
Capital Costs ◽  
Microwave Vacuum Drying ◽  
Fill Volume

AbstractVial-based lyophilization for biopharmaceuticals has been an indispensable cornerstone process for over 50 years. However, the process is not without significant challenges. Capital costs to realize a lyophilized drug product facility, for example, are very high. Similarly, heat and mass transfer limitations inherent in lyophilization result in drying cycle on the order of several days while putting practical constraints on available formulation space, such as solute mass percentage or fill volume in a vial. Through collaboration with an external partner, we are exploring microwave vacuum drying (MVD) as a faster drying process to vial lyophilization wherein the heat transfer process occurs by microwave radiation instead of pure conduction from the vial. Drying using this radiative process demonstrates greater than 80% reduction in drying time over traditional freeze-drying times while maintaining product activity and stability. Such reduction in freeze-drying process times from days to several hours is a welcome change as it enables flexible manufacturing by being able to better react to changes either in terms of product volume for on-demand manufacturing scenarios or facilities for production (e.g., scale-out over scale-up). Additionally, by utilizing first-principle modeling coupled with experimental verification, a mechanism for faster drying times associated with MVD is proposed in this article. This research, to the best of our knowledge, forms the very first report of utilizing microwave vacuum drying for vaccines while utilizing the power of simplified models to understand drying principles associated with MVD.

scholarly journals Application of sonication and freezing as initial treatments before microwave-vacuum drying of cranberries

2019 ◽  
Vol 2 (22) ◽  
pp. 151-167 ◽  
Author(s):  
Izabela Staniszewska ◽  
Szymon Staszyński ◽  
Magdalena Zielińska
Keyword(s):  
Thermal Conductivity ◽  
Thermal Properties ◽  
Thermal Diffusivity ◽  
Physical Properties ◽  
Color Change ◽  
Vacuum Drying ◽  
Drying Time ◽  
Dried Fruits ◽  
Microwave Vacuum Drying ◽  
Drying Kinetic

The aim of study was to determine the influence of sonication and freezing on the kinetic of the microwave-vacuum drying, energy consumption and physical properties of whole cranberries as well as evaluate the applicability of sonication instead of freezing in order to change their physical properties and the drying kinetic of whole cranberries. Microwave-vacuum drying of whole cranberries with/without initial treatments took from 12 ± 1 to 14.5 ± 0.5 minutes. All of treatments did not significantly shorten the drying time of cranberries. However, they increased SMER values even by 31%. Despite of cryogenic freezing, all of treatments significantly increased the values of Dew. Sonication combined with drying allowed to obtain dried berries characterized by the lowest cohesiveness (0.19±0.02), springiness (0.62±0.02) and chewiness (3.4±0.8 N), while cryogenic freezing combined with drying allowed to obtain dried fruits characterized by highest springiness (0.75±0.03) and low chewiness (3.3±0.5 N). The highest lightness (32.2±0.7), redness (32.6±0.8), and yellowness (11.1±0.7) were found for fruits subjected to initial convective freezing before drying. The efficiency of sonication in color change was comparable to cryogenic freezing and much lower than convective freezing. All of initial treatments increased such thermal properties of dried cranberries as thermal conductivity and thermal diffusivity.

scholarly journals Optimization of Microwave Vacuum Drying and Pretreatment Methods for Polygonum cuspidatum

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Wanxiu Xu ◽  
Guanyu Zhu ◽  
Chunfang Song ◽  
Shaogang Hu ◽  
Zhenfeng Li
Keyword(s):  
Mathematical Models ◽  
Scale Up ◽  
Drying Kinetics ◽  
Vacuum Drying ◽  
Orthogonal Experimental Design ◽  
Polygonum Cuspidatum ◽  
Drying Time ◽  
Microwave Vacuum Drying ◽  
Thin Layer Drying ◽  
Drying System

This study was conducted to optimize the drying process of Polygonum cuspidatum slices using an orthogonal experimental design. The combined effects of pretreatment methods, vacuum pressure and temperature of inner material, drying kinetics, color value, and retention of the indicator compounds were investigated. Seven mathematical models on thin-layer drying were used to study and analyze the drying kinetics. Pretreatment method with blanching for 30 s at 100°C increased the intensity of the red color of P. cuspidatum slices compared with other pretreatment methods and fresh P. cuspidatum slices. P. cuspidatum slices dried at 60°C retained more indicator compounds. Furthermore, microwave pretreatment methods, followed by microwave vacuum for 200 mbar at 50°C, resulted in high concentration of indicator compounds, with short drying time and less energy. This optimized condition for microwave vacuum drying and pretreatment methods would be useful for processing P. cuspidatum. The Newton, Page, and Wang and Singh models slightly fitted the microwave vacuum drying system. The logarithmic, Henderson and Pabis, two-term, and Midilli et al. models can be used to scale up the microwave vacuum drying system to a commercial scale. The two-term and Midilli et al. models were the best fitting mathematical models for the no-pretreatment case at 600 mbar and 60°C.

scholarly journals Freeze-drying techniques for preserving aphids (Homoptera, Aphidodea)

1994 ◽  
Vol 5 (2) ◽  
pp. 105-113 ◽  
Author(s):  
Anders Albrecht
Keyword(s):  
Freeze Drying ◽  
Body Shape ◽  
Vacuum Drying ◽  
Drying Methods ◽  
Drying Time ◽  
Freeze Dried ◽  
Drying Techniques ◽  
Wax Coating ◽  
And Storage

Techniques for collection, preparation and storage of freeze-dried aphid samples, including galls, are described. Freeze-drying can be done with the aid of a home freezer, a drying agent, and suitable containers alone, but drying time can be reduced considerably with cheap and simple vacuum drying equipment. Freeze-drying methods have several advantages compared with traditional mounting techniques. Body shape, colours, wax coating and microsculpture are excellently preserved. The labour required per sample, for preparation as well as for identification, is reduced to a minimum, and complete colony samples can be stored as entities. Aspects of practical handling and study of freeze-dried aphid samples are discussed.

scholarly journals Effects of sonication and freezing on the color, mechanical and thermophysical properties of osmo-microwave-vacuum dried cranberries

2020 ◽  
Author(s):  
Izabela Anna Staniszewska ◽  
Szymon Staszyński ◽  
Magdalena Zielińska
Keyword(s):  
Thermal Conductivity ◽  
Heat Capacity ◽  
Thermal Diffusivity ◽  
Moisture Diffusion ◽  
Moisture Diffusivity ◽  
Microwave Drying ◽  
Vacuum Drying ◽  
Volumetric Heat Capacity ◽  
Drying Time ◽  
Microwave Vacuum Drying

The aim of study was to determine the effects of sonication (S), convective freezing (F), convective freezing preceded by sonication (SF) as well as cryogenic freezing (N) on the osmo-microwave-vacuum drying kinetics, energy usage and properties of dried cranberries such as moisture content, moisture diffusion, water activity, density, porosity, thermal conductivity, thermal diffusivity, volumetric heat capacity, lightness, redness, yellowness, total differences in color, saturation and hue, hardness, cohesiveness, springiness, and chewiness. Osmo-microwave-vacuum drying of cranberries took from 13.5 to 16.0 min. All initial treatments increased the moisture diffusivity and thus reduced the drying time. The most energy effective method was osmo-microwave-vacuum drying preceded by sonication (S) of fruits. Osmo-microwave-drying of cranberries subjected to convective freezing preceded by sonication (SF) resulted in the highest lightness (32.5 ± 0.5), redness (33.9 ± 0.7), and yellowness (11.3 ± 0.5) of fruits, as well as the lowest cohesion (the lowest resistant to stress associated with manufacturing, packaging, storage, and delivery). The lowest hardness, i.e. 12.3 ± 0.4 N and the highest cohesiveness and springiness, i.e. 0.38 ± 0.02 and 0.74 ± 0.03 of dried fruits, were noted for berries subjected to initial cryogenic freezing (N). Cryogenic freezing (N) combined with osmo-microwave-vacuum drying resulted in the largest color changes of fruits and the highest thermal conductivity. Sonicated and convectively frozen (SF) fruits were characterized by the highest thermal diffusivity. Sonication (S), convective freezing (F) and their combination (SF) significantly reduced the volumetric heat capacity of cranberry fruits.

Study on the Kinetics and Optimization of Technological Process of Microwave - Vacuum Drying of Bleached Shellac

2011 ◽  
Vol 418-420 ◽  
pp. 94-101
Author(s):  
Hong Zhang ◽  
Lian Song Yu ◽  
Hua Zheng ◽  
Ying Feng ◽  
Xiao Ming Chen ◽  
...  
Keyword(s):  
Technological Process ◽  
Production Efficiency ◽  
Vacuum Drying ◽  
Optimal Conditions ◽  
Drying Time ◽  
Microwave Vacuum Drying ◽  
Drying Model ◽  
Kinetics Of ◽  
Bleached Shellac ◽  
Drying Curve

To enhance the product quality and production efficiency, we studied the kinetics of the microwave - vacuum drying of bleached shellac and optimized its technological process. Through the test, the drying curve, drying rate curve and drying constant of the bleached shellacs under different pressure were obtained and through the drying curve fitting, the drying model was obtained. And through the optimization test, the optimal conditions for the microwave - vacuum drying of bleached shellac were obtained, that is, pressure of 3000Pa, microwave power of 700W and drying time of 25min, then the products of color index of 0.9, water content of 0.0296 kg water / kg dry materials were obtained.

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