scholarly journals Experimental research Assessment of apoptosis, MMP-1, MMP-3, TIMP-2 expression and mechanical and biochemical properties of the fresh rabbit’s medial meniscus stored two weeks under tissue culture condition

2014 ◽  
Vol 1 ◽  
pp. 167-173 ◽  
Author(s):  
Tomasz J. Zwierzchowski ◽  
Olga Stasikowska-Kanicka ◽  
Jolanta Janus ◽  
Włodzimierz Konecki ◽  
Marian Danilewicz ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Experimental Research ◽  
Culture Condition ◽  
Medial Meniscus ◽  
Biochemical Properties ◽  
Research Assessment ◽  
Tissue Culture Condition

Enhancing Biosynthesis of Flavonol Protective Biomaterials Using FLS Transgenic Plants

2017 ◽  
Vol 866 ◽  
pp. 29-32
Author(s):  
Darin Dangrit ◽  
Kanokporn Sompornpailin
Keyword(s):  
Tissue Culture ◽  
Transgenic Plants ◽  
Photosynthetic Pigment ◽  
Flavonoid Biosynthesis ◽  
Carotenoid Pigments ◽  
Chlorophyll B ◽  
Wild Type ◽  
Flavonoid Synthesis ◽  
Transgenic Lines ◽  
Tissue Culture Condition

Flavonol synthase (FLS) gene encodes an enzyme that is involved in conversion substrates into flavonols, quercetin and kaempferol. These substances are a subgroup of flavonoids which have an important role in both plant and human health. Many environmental factors such as temperature, pH and UV-A radiation have been studied and presented relationship with flavonoid synthesis. In this experiment, the combination of visible and UV-A lights was used as factors for elevating flavonoid biosynthesis of wild type (WT) plant and two lines of FLS transgenic plant under tissue culture condition. Both transgenic lines significantly enhanced the accumulation of quercetin and kaempferol substances nearly one fold higher than WT plant did. The photosynthetic pigment levels of chlorophyll A, chlorophyll B and carotenoid in transgenic lines are in the range 45.20-46.88, 16.34-17.04 and 13.63-13.46, while those of WT plants are 35.93, 13.18 and 10.55 (µg/g FW), respectively. Therefore, FLS transgenic plants containing high flavonol content showed a better in the protection photosynthetic pigments by less reductions of chlorophyll and carotenoid pigments.

Influence of canine recombinant somatotropin hormone on biomechanical and biochemical properties of the medial meniscus in stifles with altered stability

2002 ◽  
Vol 63 (3) ◽  
pp. 419-426 ◽  
Author(s):  
Thomas J. Noone ◽  
Darryl L. Millis ◽  
Donna L. Korvick ◽  
Kyriacos Athanasiou ◽  
James L. Cook ◽  
...  
Keyword(s):  
Medial Meniscus ◽  
Biochemical Properties ◽  
Recombinant Somatotropin

scholarly journals STUDY ON GROWTH OF RICKETTSIAE

1959 ◽  
Vol 109 (3) ◽  
pp. 271-292 ◽  
Author(s):  
Zanvil A. Cohn ◽  
F. Marilyn Bozeman ◽  
Janis M. Campbell ◽  
James W. Humphries ◽  
Thomas K. Sawyer
Keyword(s):  
Tissue Culture ◽  
Divalent Cations ◽  
Biochemical Properties ◽  
Metabolic Inhibitors ◽  
Penetration Process ◽  
Factors Affecting ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Fluid Environment

A system has been described in which the penetration of Rickettsia tsutsugamushi (Karp strain) into tissue culture cells can be quantitated, and the factors affecting this process studied. The results indicated that rickettsial penetration in vitro depended largely on the viability of the organisms. Certain components of the fluid environment such as the divalent cations and protein were found to be of importance. The temperature dependence of the penetration process was found to vary with the nature of the suspending medium. A number of compounds related to L-glutamic acid enhanced penetration, whereas metabolic inhibitors depressed this process. Aureomycin at concentrations between 50 and 250 µg./ml. inhibited the penetration of rickettsiae while chloramphenicol at similar concentrations was ineffective. The results are discussed in terms of the biological and biochemical properties of this group of agents.

In vitro Tissue Culture of Brassica oleracea var Italica Curd

2013 ◽  
Vol 6 (1) ◽  
Author(s):  
ALMA B. SEGISMUNDO ◽  
MAY EVELIA V. RUADAP
Keyword(s):  
Tissue Culture ◽  
Experimental Research ◽  
Plant Tissue Culture ◽  
Culture Medium ◽  
Fungal Infections ◽  
Ms Medium ◽  
High Survivorship ◽  
Culture Technology ◽  
Observation Period

This experimental research determined the feasibility of micropropagating in vitro,broccoli from its curd through plant tissue culture technology to be able to produceplants that are free from any disease. The growth of broccoli (Brassica oleraceavar Italica curd in vitro was measured in terms of organ formation and the cultures’average height (cm) after the observation period. It also determined the percentsurvivorship of the plant cultures after the observation period. The method usedwas adopted from Balcita (2011) utilizing the modified Murashige and Skoog (MS)medium. Result of the study show that broccoli plantlets grew from the curd after asix-week observation period. Addition of thiamine in the culture medium protectedthe cultures from bacterial or fungal infections, thus coming up with very healthyplantlets and a high survivorship of the cultures was also observed. In conclusion,propagation of broccoli curd in vitro is feasible using the modified Murashige andSkoog (MS) medium; plantlets with roots, stem, leaves, and curd with florets can grow directly from the broccoli curd; and there is a high percent survivorship in thecultures with the addition of thiamine in the culture medium.

Quantitation of HIV-1 activity in tissue culture supernatants: effects of culture condition on syncytial assays and virus production

1989 ◽  
Vol 24 (1-2) ◽  
pp. 67-76 ◽  
Author(s):  
G. Spickett ◽  
R.E. Beattie ◽  
L. Bountiff ◽  
A.G. Dalgleish ◽  
A.D.B. Webster
Keyword(s):  
Tissue Culture ◽  
Culture Condition ◽  
Virus Production ◽  
Culture Supernatants ◽  
Hiv 1

Tissue section lifting for electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 86-87
Author(s):  
Adrian F. van Dellen
Keyword(s):  
Infectious Disease ◽  
Electron Microscopy ◽  
Tissue Culture ◽  
Organ System ◽  
Surrounding Tissue ◽  
Paraffin Sections ◽  
Surface Areas ◽  
Specific Determination ◽  
High Degree ◽  
Accuracy And Repeatability

The morphologic pathologist may require information on the ultrastructure of a non-specific lesion seen under the light microscope before he can make a specific determination. Such lesions, when caused by infectious disease agents, may be sparsely distributed in any organ system. Tissue culture systems, too, may only have widely dispersed foci suitable for ultrastructural study. In these situations, when only a few, small foci in large tissue areas are useful for electron microscopy, it is advantageous to employ a methodology which rapidly selects a single tissue focus that is expected to yield beneficial ultrastructural data from amongst the surrounding tissue. This is in essence what "LIFTING" accomplishes. We have developed LIFTING to a high degree of accuracy and repeatability utilizing the Microlift (Fig 1), and have successfully applied it to tissue culture monolayers, histologic paraffin sections, and tissue blocks with large surface areas that had been initially fixed for either light or electron microscopy.

Recent Studies on a Murine Leukemia Virus Grown in Tissue Culture

1967 ◽  
Vol 25 ◽  
pp. 106-107
Author(s):  
L. Z. de Tkaczevski ◽  
E. de Harven ◽  
C. Friend
Keyword(s):  
Tissue Culture ◽  
Solid Tumors ◽  
Murine Leukemia Virus ◽  
Leukemia Virus ◽  
Supernatant Fluid ◽  
Virus Particles ◽  
Friend Leukemia ◽  
Type C ◽  
Leukemia Viruses ◽  
Murine Leukemia

Despite extensive studies, the correlation between the morphology and pathogenicity of murine leukemia viruses (MLV) has not yet been clarified. The virus particles found in the plasma of leukemic mice belong to 2 distinct groups, 1 or 2% of them being enveloped A particles and the vast majority being of type C. It is generally believed that these 2 types of particles represent different phases in the development of the same virus. Particles of type A have been thought to be an earlier form of type C particles. One of the tissue culture lines established from Friend leukemia solid tumors has provided the material for the present study. The supernatant fluid of the line designated C-1A contains an almost pure population of A particles as illustrated in Figure 1. The ratio is, therefore, the reverse of what is unvariably observed in the plasma of leukemic mice where C particles predominate.

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