A comparison of the effect of chlorhexidine and collagen cross-linking
agent (oak extract) used for different times on composite-dentine
micro tensile bond strength

Recent studies have reported collagen cross-linking after exposure to riboflavin followed by ultraviolet-A (UVA) exposure. This study is the first to investigate the effect of a riboflavin-containing primer on adhesive interface stability and dentinal matrix metalloproteinase activity. Human dentin was etched with 35% phosphoric acid, treated with 0.1% riboflavin, exposed to UVA for 2 min, and bonded with a two-step etch-and-rinse adhesive. Adhesive was applied to control specimens without riboflavin/UVA. Specimens were subjected to microtensile bond strength tests and pulled to failure after storage for 24 hrs, 6 mos, or 1 yr. Interfacial nanoleakage was evaluated by light and transmission electron microscopy. To investigate dentinal matrix metalloproteinase activity, we performed correlative zymographic assays on protein extracts obtained from phosphoric-acid-etched dentin powder with or without riboflavin/UVA treatment and XP Bond. Ultraviolet-activated riboflavin treatment increased the immediate bond strength to dentin at all aging intervals (p < 0.05 vs. control) and decreased interfacial nanoleakage in aged specimens (1 yr; p < 0.05). Zymograms revealed that riboflavin/UVA pre-treatment inhibited dentinal matrix metalloproteinase activity (especially MMP-9). In conclusion, dentinal collagen cross-linking induced by riboflavin/UVA increased immediate bond strength, stabilized the adhesive interface, and inhibited dentin matrix metalloproteinases, thereby increasing the durability of resin-dentin bonds.

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The Efficacy of Riboflavin for Collagen Cross-Linking and Optimizing the Bond Strength of an Etch and Rinse Adhesive System to Dentin

Avicenna Journal of Dental Research ◽

10.5812/ajdr.13254 ◽

2017 ◽

Vol 9 (3) ◽

pp. e13254-e13254

Author(s):

Shahin Kasraei ◽

Mona Malek ◽

Zahra Khamverdi ◽

Maryam Mojtahedi

Keyword(s):

Bond Strength ◽

Adhesive System ◽

Cross Linking ◽

Etch And Rinse ◽

Collagen Cross Linking

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Shear Bond Strength of Composite to Deep Dentin After Treatment With Two Different Collagen Cross-linking Agents at Varying Time Intervals

Operative Dentistry ◽

10.2341/11-232-l ◽

2012 ◽

Vol 37 (5) ◽

pp. 485-491 ◽

Cited By ~ 9

Author(s):

S Srinivasulu ◽

S Vidhya ◽

M Sujatha ◽

S Mahalaxmi

Keyword(s):

Bond Strength ◽

Surface Treatment ◽

Shear Bond Strength ◽

Sodium Ascorbate ◽

Cross Linking ◽

Time Intervals ◽

Group Iii ◽

Dentin Surface ◽

Group I ◽

Collagen Cross Linking

SUMMARY Objective This in vitro study evaluated the shear bond strength of composite resin to deep dentin using a total etch adhesive after treatment with two collagen cross-linking agents at varying time intervals. Materials and Methods Thirty freshly extracted human maxillary central incisors were sectioned longitudinally into equal mesial and distal halves (n=60). The proximal deep dentin was exposed, maintaining a remaining dentin thickness (RDT) of approximately 1 mm. The specimens were randomly divided into three groups based on the surface treatment of dentin prior to bonding as follows: group I (n=12, control): no prior dentin surface treatment; group II (n=24): dentin surface pretreated with 10% sodium ascorbate; and group III (n=24): dentin surface pretreated with 6.5% proanthocyanidin. Groups II and III were further subdivided into two subgroups of 12 specimens each, based on the pretreatment time of five minutes (subgroup A) and 10 minutes (subgroup B). Shear bond strength of the specimens was tested with a universal testing machine, and the data were statistically analyzed. Results Significantly higher shear bond strength to deep dentin was observed in teeth treated with 10% sodium ascorbate (group II) and 6.5% proanthocyanidin (group III) compared to the control group (group I). Among the collagen cross-linkers used, specimens treated with proanthocyanidin showed significantly higher shear bond strength values than those treated with sodium ascorbate. No significant difference was observed between the five-minute and 10-minute pretreatment times in groups II and III. Conclusion It can be concluded that dentin surface pretreatment with both 10% sodium ascorbate and 6.5% proanthocyanidin resulted in significant improvement in bond strength of resin composite to deep dentin.

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Collagen cross-linking agents + dimethyl sulfoxide improving the adhesive properties of erosive lesion dentin

Brazilian Dental Journal ◽

10.1590/0103-6440202104595 ◽

2021 ◽

Vol 32 (6) ◽

pp. 93-106

Author(s):

Fabiana Suelen Figueredo de Siqueira ◽

Anna Luiza Szesz ◽

Pedro Henrique de Aguiar Moreira ◽

Lucila Cristina Rodrigues Araujo ◽

Alessandra Reis ◽

...

Keyword(s):

Bond Strength ◽

Silver Nitrate ◽

Dimethyl Sulfoxide ◽

Soft Drink ◽

Degree Of Conversion ◽

Cross Linking ◽

Microtensile Bond Strength ◽

Etch And Rinse ◽

Collagen Cross Linking

Abstract To investigate the effect of the dimethyl sulfoxide combined with cross-linking agents on microtensile bond strength, silver nitrate penetration and in situ degree of conversion analysis of adhesives to the erosive dentin treatment with Cola-based soft drink. One hundred and sixty-six molars were assigned to 20 groups: (1) Treatment: Sound dentin; Erosive dentin; Erosive dentin treated with primer of dimethyl sulfoxide; Erosive dentin treated with DMSO primer containing proanthocyanidin and rivoflavin; (2) Adhesive systems: iBond Universal and Scotchbond Universal; and (3) adhesive strategy: etch-and-rinse or self-etch strategy. After restoration, specimens were sectioned into sticks to be tested. The data from microtensile bond strength (MPa), silver nitrate penetration (%) and in situ degree of conversion (%) were analyzed by (three- and two-factor ANOVA; Tukey's test α=5%). The application of dimethyl sulfoxide combined of not with cross-linkers improved all properties evaluated when compared to only erosive dentin treatment with Cola-based soft drink. However, only when dimethyl sulfoxide was combined to cross-linkers, the values of the microtensile bond strength, silver nitrate penetration and in situ degree of conversion in erosive dentin treatment with Cola-based soft drink was similar to sound dentin, for both adhesives and adhesive strategies. The application of dimethyl sulfoxide combined with the collagen cross-linking agent contributed to increasing the bond strength and degree of conversion in erosive lesion dentin, at the same time that significantly reduction of nanoleakage in this substrate.

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Comparative evaluation of the effect of decontamination protocol on the shear bond strength of eighth generation bonding agent to contaminated dentin: an in vitro study

Medicine and Pharmacy Reports ◽

10.15386/mpr-1488 ◽

2020 ◽

Author(s):

Manasi Kewlani ◽

Suparna Ganguly Saha ◽

Anuj Bhardwaj ◽

Mainak Kanti Saha ◽

Prashansa Vijaywargiya ◽

...

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Composite Resin ◽

In Vitro Study ◽

Bonding Agent ◽

Hemostatic Agent ◽

Sodium Ascorbate ◽

Cross Linking ◽

Collagen Cross Linking

Introduction. This in vitro study aims to evaluate and compare the effect of decontamination protocol - based on sodium hypochlorite - NaOCl, as a decontaminating agent, followed by the application of a collagen cross-linking agent like sodium ascorbate [Na Ascorbate] or Proanthocyanidin [PA]) on the shear bond strength of eighth generation bonding agent to contaminated dentin (saliva, blood and blood followed by hemostatic agent). Methodology. The buccal surfaces of extracted premolars were flattened to expose the dentine. The teeth were then randomly divided into 3 groups based on the contaminant used (saliva, blood and blood followed by hemostatic agent). Decontamination protocol was carried out with sodium hypochlorite followed by application of either of the two collagen cross-linking agents (sodium ascorbate/proanthocyanidin) in each group. FuturaBond DC (eighth generation) bonding agent was used to bond composite to dentin surfaces which was subjected to shear bond strength testing. Data was analyzed by one-way ANOVA and Tukey test. Results All the groups (contaminated with saliva, blood, or hemostatic agent) showed a decrease in shear bond strength of composite resin to dentin. The shear bond strength of composite resin to dentin was least affected by contamination with saliva followed by contamination with blood and was most affected by contamination with a hemostatic agent. The decontamination protocol carried out showed that the combination of NaOCl and Proanthocyanidin improved the shear bond strength of composite to dentin significantly more than the combination of NaOCl and sodium ascorbate or ofsodium hypochlorite used alone.

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