Comparative evaluation of the effect of decontamination protocol on the shear bond strength of eighth generation bonding agent to contaminated dentin: an in vitro study

Introduction. This in vitro study aims to evaluate and compare the effect of decontamination protocol - based on sodium hypochlorite - NaOCl, as a decontaminating agent, followed by the application of a collagen cross-linking agent like sodium ascorbate [Na Ascorbate] or Proanthocyanidin [PA]) on the shear bond strength of eighth generation bonding agent to contaminated dentin (saliva, blood and blood followed by hemostatic agent). Methodology. The buccal surfaces of extracted premolars were flattened to expose the dentine. The teeth were then randomly divided into 3 groups based on the contaminant used (saliva, blood and blood followed by hemostatic agent). Decontamination protocol was carried out with sodium hypochlorite followed by application of either of the two collagen cross-linking agents (sodium ascorbate/proanthocyanidin) in each group. FuturaBond DC (eighth generation) bonding agent was used to bond composite to dentin surfaces which was subjected to shear bond strength testing. Data was analyzed by one-way ANOVA and Tukey test. Results All the groups (contaminated with saliva, blood, or hemostatic agent) showed a decrease in shear bond strength of composite resin to dentin. The shear bond strength of composite resin to dentin was least affected by contamination with saliva followed by contamination with blood and was most affected by contamination with a hemostatic agent. The decontamination protocol carried out showed that the combination of NaOCl and Proanthocyanidin improved the shear bond strength of composite to dentin significantly more than the combination of NaOCl and sodium ascorbate or ofsodium hypochlorite used alone.

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Impact of Various Cross Linking Agents on Dentin Adhesive Systems - an In-vitro Study

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i55a33810 ◽

2021 ◽

pp. 86-92

Author(s):

Ganapathi Vasavi Prasanna ◽

Vemareddy Rajasekhar ◽

Someshwar Battu ◽

Korrai Balaraju ◽

Seera Sudhakar Naidu ◽

...

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Composite Resin ◽

Group Iv ◽

Bonding Agent ◽

Sodium Ascorbate ◽

Cross Linking ◽

Group Iii ◽

Group I ◽

Self Etch

Aims and Objectives: The main aim of this study is to compare and evaluate the shear bond strength of composite resin using a total etch adhesive and self-etch adhesive after treatment with different collagen cross-linking agents. Materials and Methods: Forty freshly extracted human maxillary central incisors were taken. The proximal dentin was exposed, cavities were prepared on mesial and distal of each tooth. The specimens were randomly divided into two groups based on bonding agent applied-Group A and Group B i.e universal bonding agent and all in one bonding agent respectively and are subdivided into four groups based on the surface treatment of dentin. Group I A (n=10) Control-total etch(CTE); Group II A (n=10) Sodium Ascorbate-total etch (STE); Group III A (n=10)- Proanthocyanidin total etch(PTE); Group IV A (n=10)- Chitosan total etch (CHTE); Group I B (n=10) Control-self etch (CSE); Group II B (n=10) Sodium Ascorbate-self etch(SSE); Group III B (n=10)- Proanthocyanidin self-etch (PSE); and Group IV B (n=10) - Chitosan self-etch (CHSE). Shear bond strength of the specimens are tested with universal testing machine, and the data was statistically analysed with one way ANOVA. Results: Significantly higher shear bond strength to dentin was observed in teeth treated with 6.5% Proanthocyanidin Total etch, 10% Sodium Ascorbate Total etch and 1% Chitosan acetate compared to the control group. No significant difference was seen with self-etch treated groups. Conclusion: Dentin surface pretreatment with 6.5% Proanthocyanidin, 10% Sodium Ascorbate and 1% Chitosan acetate results in significant enhancement in bond strength of composite resin to deep dentin.

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Composite resin bond strength to primary dentin prepared with ER, CR: YSSG laser

Journal of Clinical Pediatric Dentistry ◽

10.17796/jcpd.30.1.el385u211tnu2574 ◽

2006 ◽

Vol 30 (1) ◽

pp. 45-50 ◽

Cited By ~ 16

Author(s):

Eric Sung ◽

Torin Chenard ◽

Angelo Caputo ◽

Michael Amodeo ◽

Evelyn Chung ◽

...

Keyword(s):

Bond Strength ◽

Hybrid Composite ◽

Shear Bond Strength ◽

Composite Resin ◽

In Vitro Study ◽

Vitro Study ◽

Bonding Agent ◽

Bond Strengths

This in vitro study evaluated the shear bond strength of a hybrid composite resin bonded to primary dentin prepared with an Er,Cr:YSGG hydrokinetic laser compared to conventional bur prepared primary dentin. The results suggest that primary dentin surfaces treated with the Er,Cr:YSGG laser, with or without etching, may provide comparable or increased composite resin bond strengths depending upon bonding agent used.

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Shear Bond Strength of Composite Resin and Conventional Flowable Composite in Primary Molars Prepared by Bond prep mode and Cutting mode Er, Cr:YSGG Laser (an in vitro study)

Egyptian Dental Journal ◽

10.21608/edj.2019.74000 ◽

2019 ◽

Vol 65 (4) ◽

pp. 3241-3248

Author(s):

Ayman Sabah

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Composite Resin ◽

In Vitro Study ◽

Vitro Study ◽

Primary Molars ◽

Flowable Composite

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Effect of nonthermal atmospheric plasma on the shear bond strength of composite resin after using different tooth-whitening systems: An in vitro study

Journal of Conservative Dentistry ◽

10.4103/jcd.jcd_612_20 ◽

2021 ◽

Vol 24 (2) ◽

pp. 143

Author(s):

KommineniHarika Chowdary ◽

Bolla Nagesh ◽

PraveenKumar Gali ◽

Tammineedi Sravanthi ◽

LakshmiBhavani Potru ◽

...

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Composite Resin ◽

In Vitro Study ◽

Vitro Study ◽

Atmospheric Plasma ◽

Tooth Whitening

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Effect of Antioxidant and Buffering Agent Mixture on Dentin Shear Bond Strengths of Laminate Veneer and pH Change of External Medium After Intracoronal Bleaching

Journal of Advanced Oral Research ◽

10.1177/23202068211039711 ◽

2021 ◽

pp. 232020682110397

Author(s):

Zeliha Gonca Bek Kurklu

Keyword(s):

Hydrogen Peroxide ◽

Bond Strength ◽

Calcium Hydroxide ◽

Shear Bond Strength ◽

External Medium ◽

In Vitro Study ◽

Sodium Ascorbate ◽

Ph Change ◽

Buffering Agent

Aim: To investigate the effect of sodium ascorbate (SA), an antioxidant and calcium hydroxide (Ca(OH)2), a buffering agent mixture, on the shear bond strength of a luting resin to dentin, and pH changes of the extraradicular medium solution after intracoronal bleaching with hydrogen peroxide. Materials and Methods: In this in vitro study, 60 human maxillary central incisors were randomly assigned into six groups ( n: 10): Group 1: restored, no bleach; group 2: bleached, bonded immediately; group 3: bleached, treated with 10% SA applications before bonding; group 4: bleached, treated with calcium hydroxide applications before bonding; group 5: bleached, treated with CaOH2 and SA mixture applications before bonding; group 6: delay bonding by seven days. For bleach treatment in groups 2 to 6, intracoronal bleaching was performed with 35% hydrogen peroxide for four days. SA or CaOH2 or mixture of SA and CaOH2 were left in the access cavity for four days. Hybrid blocks, 4 mm × 4 mm wide × 2–mm thick, were luted with resin cements on dentin surface and then the specimens were subjected to shear bond strength testing. Analysis of variance and Tukey post hoc multiple comparison tests were applied ( P < .05). Results: G2 and G4 showed significantly low bond strength values and they were lower than the other groups ( P < .05). There were no significant differences between G1, G3, and G6 ( P > .05). The pH values had risen markedly in the groups containing calcium hydroxide (G4 and G5) after four days ( P < .05). Conclusion: The application of 10% SA or 10% SA and CaOH2 mixture were effective at reversing bleaching effects on bond strength. CaOH2 or CaOH2 and 10% SA mixture increased the external medium pH and were effective for alkalinization after intracoronal bleaching.

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Effect of Bonding Agent on Retention of Different Sealants: An in Vitro Study

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4625-45.3.6 ◽

2021 ◽

Vol 45 (3) ◽

pp. 177-185

Author(s):

Moaz H Attar ◽

Medhat A Abdallah ◽

Hussein A Alharthy ◽

Omar A El Meligy

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

In Vitro Study ◽

Bonding Agent ◽

Micro Ct ◽

Group Iii ◽

Group I ◽

Significant Difference ◽

Sealant Retention

Objectives: To investigate the effect of Co-curing versus Staged-curing and No-bonding on retention of different resin-based sealants (RBS). Study design: For shear bond strength (SBS) and microleakage tests, 90 extracted premolars were divided equally into 3 groups (I, II, III). Each group was further subdivided equally into 3 subgroups (a, b, c). No-bonding subgroups did not receive a bonding agent, Staged-curing subgroups received a bonding agent that was cured before sealant application, while Co-curing subgroups received a bonding agent that was cured after sealant application. Seal-it was applied for group I, Helioseal-F for group II and Clinpro for group III. SBS buttons were tested using Instron machine, while microleakage specimens were examined using micro-CT. Results: Clinpro showed the highest SBS values in Staged-curing and No-bonding groups (8.72±2.39, 12.51±3.16) respectively. Staged-curing was significantly greater in SBS values than those for other groups (P<0.05). There was a significant difference in microleakage values of Staged-curing among different RBS (P = 0.003), while there was no significant difference in values of No-bonding and Co-curing among different RBS (P = 0.541, P = 0.521). Conclusions: The use of a bonding agent as Staged-curing was more effective in improving sealant retention than No-bonding and Co-curing.

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Effect of Contamination with Hemostatic Agent on Shear Bond Strength of Composite to Dentin Using G-Premio and Single Bond Universal Adhesives

Frontiers in Dentistry ◽

10.18502/fid.v18i27.6937 ◽

2021 ◽

Author(s):

Zahra Khamverdi ◽

Naemeh Karimian ◽

Maryam Farhadian ◽

Hamed Gheitouli

Keyword(s):

Bond Strength ◽

Shear Bond Strength ◽

Testing Machine ◽

In Vitro Study ◽

Hemostatic Agent ◽

Control Group ◽

Single Bond ◽

Failure Data ◽

Universal Adhesives

Objectives: Bond strength of composite restorations plays an important role in their success. This in vitro study evaluated the effect of a hemostatic agent on shear bond strength of universal adhesives. Materials and Methods: Thirty-six extracted human molars were used in this study. Buccal and lingual surfaces were reduced to obtain flat dentin surfaces and were ground with a silicone paper. The samples were randomly divided into three groups (n=12) based on the application of hemostatic agent: group 1: no contamination (control), group 2: aluminum chloride application, and group 3: ferric sulfate application. Each group was then divided into two subgroups (n=6) for using G-Premio and Single Bond Universal. Resin cylinders (Filtek Z550) were bonded to dentin surfaces according to the manufacturer’s instructions. After 1000 thermal cycles, shear load was applied to the specimens using a universal testing machine at a crosshead speed of 1 mm/min until failure. Data were statistically analyzed using two-way ANOVA and Tukey’s post-hoc test (α=0.05). Results: There were statistically significant differences in shear bond strength of the three main groups for both G-Premio and Single Bond Universal (P<0.05). When the adhesive systems were compared with each other, G-Premio showed higher shear bond strength than Single Bond Universal (P<0.05). Conclusion: Contamination with hemostatic agents had an adverse effect on the shear bond strength of universal adhesives. Moreover, G-Premio yielded a higher bond strength than Single Bond Universal.

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