scholarly journals Effect of UVA-activated Riboflavin on Dentin Bonding

2011 ◽  
Vol 90 (12) ◽  
pp. 1439-1445 ◽  
Author(s):  
A. Cova ◽  
L. Breschi ◽  
F. Nato ◽  
A. Ruggeri ◽  
M. Carrilho ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Phosphoric Acid ◽  
Bond Strength ◽  
Cross Linking ◽  
Adhesive Interface ◽  
Etch And Rinse ◽  
Human Dentin ◽  
Pre Treatment ◽  
Metalloproteinase Activity ◽  
Collagen Cross Linking

Recent studies have reported collagen cross-linking after exposure to riboflavin followed by ultraviolet-A (UVA) exposure. This study is the first to investigate the effect of a riboflavin-containing primer on adhesive interface stability and dentinal matrix metalloproteinase activity. Human dentin was etched with 35% phosphoric acid, treated with 0.1% riboflavin, exposed to UVA for 2 min, and bonded with a two-step etch-and-rinse adhesive. Adhesive was applied to control specimens without riboflavin/UVA. Specimens were subjected to microtensile bond strength tests and pulled to failure after storage for 24 hrs, 6 mos, or 1 yr. Interfacial nanoleakage was evaluated by light and transmission electron microscopy. To investigate dentinal matrix metalloproteinase activity, we performed correlative zymographic assays on protein extracts obtained from phosphoric-acid-etched dentin powder with or without riboflavin/UVA treatment and XP Bond. Ultraviolet-activated riboflavin treatment increased the immediate bond strength to dentin at all aging intervals (p < 0.05 vs. control) and decreased interfacial nanoleakage in aged specimens (1 yr; p < 0.05). Zymograms revealed that riboflavin/UVA pre-treatment inhibited dentinal matrix metalloproteinase activity (especially MMP-9). In conclusion, dentinal collagen cross-linking induced by riboflavin/UVA increased immediate bond strength, stabilized the adhesive interface, and inhibited dentin matrix metalloproteinases, thereby increasing the durability of resin-dentin bonds.

scholarly journals Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems

Marine Drugs ◽  
2020 ◽  
Vol 18 (5) ◽  
pp. 263 ◽  
Author(s):  
Eugenia Baena ◽  
Sandra R Cunha ◽  
Tatjana Maravić ◽  
Allegra Comba ◽  
Federica Paganelli ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Bond Strength ◽  
Adhesive Systems ◽  
Microtensile Bond Strength ◽  
Bond Stability ◽  
Etch And Rinse ◽  
Mechanical Durability ◽  
Human Dentine ◽  
Self Etch ◽  
Metalloproteinase Activity

The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive. Microtensile bond strength and interfacial nanoleakage expression of the bonded interfaces for all adhesives (with or without pretreatment with 0.1% Ch solution for 1 min and air-dried for 5 s) were analyzed immediately and after 10,000 thermocycles. Zymograms of protein extracts from human dentine powder incubated with Optibond FL and Scotchbond Universal on untreated or Ch-treated dentine were obtained to examine dentine matrix metalloproteinase (MMP) activities. The use of 0.1% Ch solution as an additional primer in conjunction with the E&R or SE adhesive did not appear to have influenced the immediate bond strength (T0) or bond strength after thermocycling (T1). Zymography showed a reduction in MMP activities only for mineralized and demineralized dentine powder after the application of Ch. Application of 0.1% Ch solution does not increase the longevity of resin–dentine bonds. Nonetheless, the procedure appears to be proficient in reducing dentine MMP activities within groups without adhesive treatments. Further studies are required to comprehend the cross-linking of Ch with dentine collagen.

Collagen cross-linking agents + dimethyl sulfoxide improving the adhesive properties of erosive lesion dentin

2021 ◽  
Vol 32 (6) ◽  
pp. 93-106
Author(s):  
Fabiana Suelen Figueredo de Siqueira ◽  
Anna Luiza Szesz ◽  
Pedro Henrique de Aguiar Moreira ◽  
Lucila Cristina Rodrigues Araujo ◽  
Alessandra Reis ◽  
...  
Keyword(s):  
Bond Strength ◽  
Silver Nitrate ◽  
Dimethyl Sulfoxide ◽  
Soft Drink ◽  
Degree Of Conversion ◽  
Cross Linking ◽  
Microtensile Bond Strength ◽  
Etch And Rinse ◽  
Collagen Cross Linking

Abstract To investigate the effect of the dimethyl sulfoxide combined with cross-linking agents on microtensile bond strength, silver nitrate penetration and in situ degree of conversion analysis of adhesives to the erosive dentin treatment with Cola-based soft drink. One hundred and sixty-six molars were assigned to 20 groups: (1) Treatment: Sound dentin; Erosive dentin; Erosive dentin treated with primer of dimethyl sulfoxide; Erosive dentin treated with DMSO primer containing proanthocyanidin and rivoflavin; (2) Adhesive systems: iBond Universal and Scotchbond Universal; and (3) adhesive strategy: etch-and-rinse or self-etch strategy. After restoration, specimens were sectioned into sticks to be tested. The data from microtensile bond strength (MPa), silver nitrate penetration (%) and in situ degree of conversion (%) were analyzed by (three- and two-factor ANOVA; Tukey's test α=5%). The application of dimethyl sulfoxide combined of not with cross-linkers improved all properties evaluated when compared to only erosive dentin treatment with Cola-based soft drink. However, only when dimethyl sulfoxide was combined to cross-linkers, the values of the microtensile bond strength, silver nitrate penetration and in situ degree of conversion in erosive dentin treatment with Cola-based soft drink was similar to sound dentin, for both adhesives and adhesive strategies. The application of dimethyl sulfoxide combined with the collagen cross-linking agent contributed to increasing the bond strength and degree of conversion in erosive lesion dentin, at the same time that significantly reduction of nanoleakage in this substrate.

scholarly journals The use of collagen cross-linking agents to enhance dentin bond strength

2009 ◽  
Vol 91B (1) ◽  
pp. 419-424 ◽  
Author(s):  
Aiman Al-Ammar ◽  
James L. Drummond ◽  
Ana Karina Bedran-Russo
Keyword(s):  
Bond Strength ◽  
Cross Linking ◽  
Collagen Cross Linking

Comparison of Shear Bonding Strength for Two Different Etching Systems in Canine and Human Dentin

2011 ◽  
Vol 28 (4) ◽  
pp. 236-241
Author(s):  
Larry Kimberlin ◽  
Phil Brown
Keyword(s):  
Bond Strength ◽  
Bonding Strength ◽  
Shear Bond Strength ◽  
Etch And Rinse ◽  
Human Dentin ◽  
Acid Etch ◽  
Molar Teeth ◽  
Bonding Shear Strength ◽  
Composite Cylinders ◽  
Shear Bonding Strength

Canine dentin has increased size and number of dentinal tubules compared to human dentin. With less intertubular surface area for bonding to composite material in dog teeth, bonding shear strength may be affected negatively. This study was designed to compare the shear bonding strength of canine and human dentin. It also compared bond strength of two different acid etch systems when used with the same composite resin. The shear bond strength of composite cylinders to dentin was measured in 30 extracted canine cuspid teeth and a similar number of human molar teeth. Shear bond strength was 40 % greater for human compared with canine dentin. This significantly different (P < 0.05) result validates the hypothesis that dentin bond strength is positively correlated with the area of intertubular dentin. Results also indicated that shear bond strength was greater in the self-etching bond system compared with the etch and rinse system, although this difference was not significant.

The Influence of Specimen Grips on the Measurement of Micro-Tensile Bond Strength to Human Dentin

2011 ◽  
Vol 492 ◽  
pp. 18-23
Author(s):  
Xin Yi Zhao ◽  
Shi Bao Li ◽  
Xu Gong
Keyword(s):  
Bond Strength ◽  
Testing Machine ◽  
Tensile Bond Strength ◽  
Cohesive Failure ◽  
Universal Testing Machine ◽  
Crosshead Speed ◽  
Adhesive Interface ◽  
Universal Testing ◽  
Multiple Beams ◽  
Human Dentin

To evaluate the effects of specimen grips on the measurement of the micro-tensile bond strength (mTBS) to dentin. Methods: Twelve extracted human molars were sectioned to expose mid-coronal dentin. Each surface was ground with 600-grit SiC paper. Four adhesives: Prime & Bond NT (Dentsply, USA), Contex (DMG, German), Adper Prompt (3M/ESPE, USA) and Clearfil S3Bond (Kuraray, Japan) were applied to the polished surfaces followed by creation of composite buildups. After 24 hr storage in 37°C water, the teeth were sectioned perpendicular to the adhesive interface to produce multiple beams of composite-bonded dentin, approximately 0.8 mm2in cross-sectioned area. Half of the specimens were attached to testing grips A which did not contain positioning pins and another half were attached to the testing grips B which contained positioning pins. All specimens were tested using a universal testing machine at a crosshead speed of 1.0 mm/min. Results: Specimens tested using the grips A presented lower mTBS than using the grips B (P<0.01). Many specimens tested using the grips A showed mix failure or cohesive failure within composite, and most of the failures were adhesive for specimens tested using the grips A. Conclusion: Specimen grips without positioning pins cannot accurately present mTBS and the grips with positioning pins can more accurately present mTBS.

Effect of Enamel Etching Time on Roughness and Bond Strength

10.2341/08-72 ◽  
2009 ◽  
Vol 34 (2) ◽  
pp. 217-222 ◽  
Author(s):  
W. W. Barkmeier ◽  
R. L. Erickson ◽  
N. S. Kimmes ◽  
M. A. Latta ◽  
T. M. Wilwerding
Keyword(s):  
Phosphoric Acid ◽  
Bond Strength ◽  
Clinical Relevance ◽  
Etching Time ◽  
Application Time ◽  
Adhesive Systems ◽  
Etch And Rinse

Clinical Relevance Traditionally, etch-and-rinse adhesive systems have used phosphoric acid to condition enamel surfaces and successfully create a strong, durable bond to resin-based materials. Newer adhesive systems now use acid monomers to bond resin materials to both enamel and dentin. These newer adhesives do not provide the same degree of bonding to enamel as etch-and-rinse systems, and extending the application time does not improve their performance.

scholarly journals Resin bond strength to water versus ethanol-saturated human dentin pretreated with three different cross-linking agents

2016 ◽  
Vol 19 (6) ◽  
pp. 555 ◽  
Author(s):  
BhuvanShome Venigalla ◽  
Pinnamreddy Jyothi ◽  
Shekhar Kamishetty ◽  
Smitha Reddy ◽  
RaviChandra Cherukupalli ◽  
...  
Keyword(s):  
Bond Strength ◽  
Cross Linking ◽  
Human Dentin

scholarly journals Influence of chlorhexidine on longitudinal bond strength to dentin: in vitro study

2017 ◽  
Vol 20 (1) ◽  
pp. 17 ◽  
Author(s):  
Beatriz Maria Fonseca ◽  
Daphne Camara Barcellos ◽  
César Rogério Pucci ◽  
Eduardo Bresciani ◽  
Maria Amélia Máximo de Araújo
Keyword(s):  
Phosphoric Acid ◽  
Bond Strength ◽  
Water Storage ◽  
In Vitro Study ◽  
Chlorhexidine Gluconate ◽  
Acid Etching ◽  
Single Bond ◽  
Etch And Rinse

<p><strong>Objective</strong>: This study evaluated the effect of 0.2% chlorhexidine gluconate solution used as an therapeutic primer on the long-term bond strength of etch-and-rinse adhesive to dentin. <strong>Material</strong> <strong>and</strong> <strong>Methods</strong>: Bovine incisors were worn to expose an area of dentin and were divided into 2 groups: Group C (Control) - acid etching with 37% phosphoric acid + Single Bond; Group CHX (0.2% CHX) - acid etching with 37% phosphoric acid + 0.2% CHX for 30 s + Single Bond. Blocks of composite were fabricated and stored for 24 h or 6 months, sectioned into beams and submitted to microtensile tests. Results were analyzed by two-way ANOVA and Tukey tests. <strong>Results</strong>: Mean (±SD) values (in MPa) were as follow: Group CHX/24h - 41.8(±2.62)A; Group C/24h - 40.8(±3.35)AB; Group CHX/6 months – 36.4(±3.52)B; Group CHX/6 months - 26.1(±1.54)C. <strong>Conclusion</strong>: CHX improve the imediatte bond strength of resin-dentin and significantly lowered the loss of bond strength after 6 months water storage as seen in the control bonds.</p><p><strong>Keywords</strong></p><p>Tensile bond strength; Dentin; Total-etch adhesives; Chlorhexidine gluconate.</p>

scholarly journals Influence of previous acid etching on interface morphology and bond strength of self-etching adhesive to cavosurface enamel

2012 ◽  
Vol 06 (01) ◽  
pp. 056-062 ◽  
Author(s):  
Adriano Fonseca Lima ◽  
Vinícius Brito da Silva ◽  
Giulliana Panfiglio Soares ◽  
Giselle Maria Marchi ◽  
Flávio Henrique Baggio Aguiar ◽  
...  
Keyword(s):  
Phosphoric Acid ◽  
Bond Strength ◽  
Adhesive System ◽  
The Self ◽  
The Other ◽  
Acid Etching ◽  
Control Group ◽  
System Control ◽  
Etch And Rinse ◽  
Group 3

ABSTRACTObjectives: The aim of this study was to evaluate the (1) bond strength of a etch-and-rinse and self-etching adhesive systems to cavosurface enamel, (2) influence of the previous acid etching with phosphoric acid 35% to the self-etching adhesive application on bond strength values, and (3) analysis of the cavosurface enamel morphology submitted to different types of conditioning, with the use of a scanning electronic microscope (SEM).Methods: Twenty four human third molars were sectioned on mesio-distal direction, resulting in two slices. The specimens were ground flat with 600-grit aluminum oxide papers, and were randomly divided into three groups: Group 1 (etch-and-rinse adhesive system (control group)), Group 2 (selfetching adhesive), and Group 3 (self-etching adhesive with previous 35% phosphoric acid-etching for 15 s). Four cylinders (0.75 mm of diameter, 1 mm height) were confectioned prior to the microshear test. Four samples for each group were prepared according the cavosurface enamel treatment and were analyzed in an SEM.Results: Group 3 had the highest values on bond strength to cavosurface enamel compared to the other two groups, which presented statistically similar values. The performance of acid etching before the application of the self-etching adhesive results in an etching pattern that is different than the other groups, favoring the adhesion to the cavosurface enamel.Conclusions: Acid etching increases the bond strength values of the self-etching adhesive to cavosurface enamel, promoting a conditioning pattern that favors the adhesion to this substrate. (Eur J Dent 2012;6:56-62)

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